LOCUS       NM_001317215            7044 bp    mRNA    linear   VRT 07-JAN-2020
DEFINITION  Xenopus tropicalis argonaute 1, RISC catalytic component (ago1),
            mRNA.
ACCESSION   NM_001317215 XM_002936214
VERSION     NM_001317215.1
KEYWORDS    RefSeq.
SOURCE      Xenopus tropicalis (tropical clawed frog)
  ORGANISM  Xenopus tropicalis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae;
            Xenopus; Silurana.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW and
            Richardson P.
  TITLE     Genetic and genomic tools for Xenopus research: The NIH Xenopus
            initiative
  JOURNAL   Dev. Dyn. 225 (4), 384-391 (2002)
   PUBMED   12454917
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CX894354.1, BC154703.1, CX935992.2 and AAMC04000002.1.
            
            On Nov 20, 2015 this sequence version replaced XM_002936214.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed/partial sample support SAMD00028290,
                              SAMD00028291 [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                CX894354.1         1-35
            36-1132             BC154703.1         1-1097
            1133-3090           BC154703.1         1112-3069
            3091-3819           CX935992.2         2-730               c
            3820-7044           AAMC04000002.1     81772788-81776012   c
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Xenopus tropicalis"
                     /mol_type="mRNA"
                     /db_xref="taxon:8364"
                     /chromosome="2"
                     /map="2"
     gene            1..7044
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /note="argonaute 1, RISC catalytic component"
                     /db_xref="GeneID:100302589"
                     /db_xref="Xenbase:XB-GENE-5933499"
     exon            1..137
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    83..85
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /note="upstream in-frame stop codon"
     CDS             113..2686
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /note="eukaryotic translation initiation factor 2C, 1;
                     argonaute RISC catalytic component 1"
                     /codon_start=1
                     /product="protein argonaute-1"
                     /protein_id="NP_001304144.1"
                     /db_xref="GeneID:100302589"
                     /db_xref="Xenbase:XB-GENE-5933499"
                     /translation="

MEAGPSSAAPSGYVPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGHERVDFEVTIPGEGKDRIFKVSIKWVAVVSWRMLHEALGSGRIQLPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADRSERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA"

     misc_feature    212..604
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    632..784
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    785..1147
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(941..943,986..988,1028..1030,1040..1042,1094..1096,
                     1115..1117,1121..1123)
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1280..2557
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1691..1693,1703..1705,1739..1750,1757..1759,
                     1781..1783,1790..1792,1802..1804,1814..1816)
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1895..1897,1901..1903,2111..2113,2525..2527)
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /note="active site"
                     /db_xref="CDD:240015"
     exon            138..321
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            322..442
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            443..624
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            625..761
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            762..896
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            897..984
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1132
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1252
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1375
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            1376..1509
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1694
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1854
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..1945
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            1946..2140
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2275
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            2276..2377
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            2378..2577
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
     exon            2578..7044
                     /gene="ago1"
                     /gene_synonym="eif2c; eif2c1; gerp95"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

agcgcatgcgcctgaagcttagagcttggtgaacaggcagctgcaacagaacagggagacagtagccacattacattgtacataaaaccagatattaagctgtgtggatgggatggaagctggaccttcttctgcagccccgagtggctatgttccacccctgcagcaagtgttccaagccccacgaaggccaggaattggtactgtggggaaacctattaaattacttgccaattactttgaggtagacattcccaagattgacgtctatcactatgaggtggatatcaaaccagataaatgtcctcgccgtgtaaacagggaagttgttgagtacatggtacagcactttaaaccacaaatttttggagacagaaagccagtgtatgatggaaagaagaatatttacactgtcacagcacttcctattggccatgaacgggttgattttgaggtgactatccccggagaagggaaggatagaatttttaaggtatctataaagtgggtggcagtggtgagttggaggatgcttcatgaggcacttgggagtggacggattcagctacccctggagtctgtgcaagctttggatgtagcaatgaggcacttggcatcaatgaggtatacccctgttggacgatctttcttctctccacctgagggctactatcaccctcttgggggtgggcgggaggtttggtttggtttccatcagtctgtgcgtccagccatgtggaacatgatgctcaatattgatgtgtcagcaactgccttctacaaagcccagcctgtcatagagttcatgtgtgaagtactggatatacgaaacattgatgaacagccaaaacctctcacagattctcagagagttcgcttcacaaaggagatcaaaggcttaaaagtagaagttacccactgtgggcagatgaagagaaaatacagggtatgcaatgttacccggaggccggctagtcatcagacgttccccttacagttggagagtggtcagactgttgaatgcacagtagcacagtattttaaacagaaatataacctacagctaaagtatccccaccttccctgtctccaggtgggccaagagcagaaacacacatacctacctctggaggtttgcaacattgtagctggccagcgatgtattaagaagctgacagacaatcagacctccactatgatcaaagctacagctcgatctgctccagacagacaagaggagatcagcaggctgatgaagaatgccagttacaatttggatccatacattcaggaatttgggatcaaagtcaaagatgacatgacagaggtcactgggcgagtcctcccagcacccattcttcagtacggagggagaaacagagcaattgcaactccaaaccaaggtgtctgggacatgcgaggaaagcagttctacaatggcattgagataaaagtctgggcaattgcatgtttcgctccacagaagcaatgcagggaagaagtgctgaaaaattttaccgaccaactccgcaaaatctcaaaagatgcaggaatgccaatccagggtcagccatgcttctgtaaatatgcacagggggcagacagtgtggagcccatgttccggcacttgaaaaacacatattctggccttcagctcatcattgttattctgccagggaaaacacctgtatatgcggaagtgaaacgtgttggggatacattgcttgggatggccactcagtgtgtgcaggtgaaaaatgtagttaagacttctcctcagacgctcagcaacctctgcctgaagatcaatgtcaagctgggtggtatcaacaacattttagtacctcatcaacggtctgcggtcttccagcagccagttatatttcttggagcagatgttacacacccacctgctggtgatggcaagaagccctctattactgcggttgttggtagcatggatgcccacccaagccggtactgtgccactgtgagagttcagcgacctcgacaggagatcattgaggacctgtcttatatggtgagggaattacttatccagttttataagtccacccgcttcaaaccaactcgaataattttctatcgtgatggggttcctgaaggacaactgccccagatcttgcattatgagttgctggcaattcgggatgcctgtattaaattggagaaggactatcagcctggaatcacgtatatagttgtgcagaaacgtcaccacacgcggctgttttgcgctgaccgcagtgagcggattggaaaaagtggaaacattccagcaggaacaactgttgacaccaacatcacacacccttttgaatttgacttttacttgtgcagccatgctggtatccagggaacaagccgcccctctcactactatgtgctttgggatgacaacagattcacagcagatgagctgcagattctcacttaccagctttgccacacctacgtgcgctgtacacgctctgtctcaattcctgcaccagcctattatgctcgattggtggcatttagagcaaggtatcacttggtggataaggaacatgacagtggagaaggcagtcatatctctggacagagcaatgggagggacccacaggccttggcaaaagcagtgcaagttcaccaggacacactgcgcacaatgtattttgcctgatgggcttcacaaagcattttaagtggataccatctattttatgactttttttttgccctaacgtgtacctcggttgctcagaagagttggggtgcctgagcactacagaagaagaggtaactctattagaaaaaacttagacgcaagtgagtccagtgggaccaacaaggagatcagaggacactgctgaagatatatatattttgcatgtttggagagaagactttttaaaaccacatacatggagagtcttcatccaaacaaaaaggaagttttgttcctgatgccacggcaaaattttacatcttgataacttgaaatgaattgtacaagtactgtgtatcaagctaagcccctttctcctcaaaaaaaggaacgatccgatatttggagtgtgaactaaagaaagaaagaagaacatttagagccttaaaatgattagtctggttttctcccctggtaataatgtagttataacgaggtaactcattgcaggctttgaatatcttcctttgtggaagctcactcaccacacatttcacatatgactcttagcacttacaacttacccagcacaaaaatatttcttatgtttctgtggatatggatagaccacctgacttcaggtcatcaagcggttgagtatgttttagaaacacgaatgcactggaaaaagaacatttaggaacttgatcattaattataattaggcaggtatttaaaattacgatatgatttatggggcatggcacagagtctaatgccaatgaagaattaaaggctcgcctattcatgtaccccaggaattgttggtgccacaaggcctttttactgttaggcaaaatggaggtgggctgcttgaagcaataggttggttttatccaatgattaactgactcctgtgtgccttcataactcagagaagatgttgatcagagctgcaaatgaacttgggtgcaaaggctgacgccactggtttaattgggtcaccggggtgggggagagaattgtgcaaagtgccttgatctttttatgctacacggggcataaatttgcatattagtgttctccagagctgcaggatggaaagtcactaaatagagtaccagcccactaggcaaaaaaaaaaaaaacgctcttaatgatgtatcctttagacatttctgcttcagtctggtttacattttattgttgtgacttacactccattgcaggtcattttaacttggagaagagctgtaggtctagcctaccactgttgattgtcaaagcactattgccagaaccaatataatacaaatgtcacagggaaatgttctttccctaaaaacactatggtcacaatttattatttttttttttgcacaaatttttgtggcaaacaccagcagaagaaaccttggtggctggatatatagataccatattatatggtgatttttaacctttttatttaaatttttatatggtaaaggtatgatgtctgcagttatcataatggctcttcttgctagtaatatcacccctcaccccttactattatccacaaaaaccaggcaaagtagctgaaagagagggagctataatattggtgccatttaatgcagtgaccttatctagctatctgtgaatctcggagaggataagaagttcctgtgttgggaatacttcattgatttttgtggtgatatttgtggtaaacagccacaaggacagtggtttttactgccaatacattttcaaaaaaaaccctcaacacaactgtgtagattcattgccatcaggggagtgtgtgccttatgctggttttgggatattaacattttagttatgagtaggattacacttttcatgtttgtctacatttcaagccactgtgaaaatgaaggctatgtaaggtaggcaaattcgccaaggaagaacatcactatgctactgcttagtatcctgatatgtacactaacaaatatgcaaaagcatggcgatttacagtctgtggatttattaacatggaaccacttggtattataatgtgttacggatctgcagcagcaaagcggttctcatttaatgggaagtcctaatttacactttttacatcttcaccagcaagcaatactttggctttttcaacagtagtgaaggggcctgttaacaacttaacccttgaactcttagccatgcattactatgggtggtgtagaaccctagtttcagtgttaaaacctgtccgtgatggctgctggctatatagtcaataaatcatagaattgttagtgataactaaagtgccaaactataaaacttttactgaggggcaggctgaagagaaaattcctgtatttttccctaaaataatggttattatggtacatgaatctgattttttgttacagaaacagaaaacctttggttcttcagatatcttgtctagagtactgaaggaagtctgcccctgctctgttgtttcttcttctgtggcaataaccaccacctacttggtagccaggctcatcataccttattttgttattatgtttttacattgtcgtgtttatattgtattttatttatttcagtaaatgcttttaatctcgatgaatttgttgccaacacagcatgtggccaagtgctataataccacaacatcaaatatatcctccgtctaattttttttaaaatctagatttatattttggtgcttttaagaccaaccaaacgttaattggccttgtattggcagaaatctgtgatgctaatttaatacattagaggtgacactacatcctaaagcctgataatgaaataaaaatgttttaaagtgctgtagtgtaaagagtatccatgtggctgtattgcttttcagtcaggatctactcagtaaagcgaggtgttgatctaggtgcctagtttagtgctatgaattctaattctggatttgtaggggaattttaaagtttattcttctgaactgttttctagtattgcctttgatgtgatatgatgaattccagcaaattacaaaagcagaaagaaagttctgtcagcaacagtgctgtgtatgatttttgcttttatataatgtgctttaaaatttattttgccatgtacttcatgtgtttcctgactgcactccaaaagcatatagcactatgcagtgttagagaagctgcactctggttcagtaagcacagtgtctagcaatgtattcatcaccagccccacatgcccagattgggaaccacaatgcaaaacctcaatcactgttacatctgtatgaacacaagtgactcggaaaagaaaaaaaaaaataatggcagccatttcagataagggcaaagatttttctgttccccgattgtccctttaacatggaaaattgtattatggggatgaaaagaaccccttcttaaagtagaatcttcccatatttttactcagaaataaaaaagctaacagtctattttatacataaaatgcttttattattcatatttataaatatgaatgataatatgatgcatgcctctgaagtatattcattaatccccaaaaatccgtggtctttatttaacaaattgtactgttctgttgatgaataggtcagttgacttggtatggcagctaccacatttacatagcaatggacttactacaacctacctgtatttaatgtataaagtctaactgctgtgttacaatccaacagaaatgtttacaaaagggtagggtacaaatatatcagaaattaatttctttctatgttctgtttacaaccaatacagatacttggtaaaagctgtaggtatctggaactagtatgtgtgcagctttgtggagtgagttatgggtaaaaaaatgttagtagcgctcagccttaacatctgtaggaaagatctttggggtacattccgctgtactggcgtttcccctgctgatacatatttttgctgcatttcttttttaatcattagccttaaaagtacttgtattaattatttttccatattatctttaaaaaaaaagagagaaatttgttcaaattattcttgtaaatgatcgatttgagacttggcttcttgtcattgttttgtcattgtaatagagtggtcacaatatatacagactaaggtataccatgcaggacaccctagtgaagaaccagattcccgtagctggtaatcatgtcttacaattatttaaccaagaatgcattgtacacatttttatatatatattgtctttacgaatgcactgaatctaggattcagtatttggccaggattcagattcggccgaatccaaaaaatcacatgactgtttgtcatgttaatacggaagtggaaaaattttcgctg

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