GGRNA Home | Help | Advanced search

2024-03-29 22:19:54, GGRNA : RefSeq release 60 (20130726)

LOCUS       NM_016077                804 bp    mRNA    linear   PRI 07-JUL-2013
DEFINITION  Homo sapiens peptidyl-tRNA hydrolase 2 (PTRH2), mRNA.
ACCESSION   NM_016077
VERSION     NM_016077.3  GI:62865877
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Chen,R., Braun,G.B., Luo,X., Sugahara,K.N., Teesalu,T. and
            Ruoslahti,E.
  TITLE     Application of a proapoptotic peptide to intratumorally spreading
            cancer therapy
  JOURNAL   Cancer Res. 73 (4), 1352-1361 (2013)
   PUBMED   23248118
  REMARK    GeneRIF: Data indicate that the cell death domain (CDD) to the
            N-terminal 62 amino acids of Bit1 was more potent in killing cells
            than the full-length Bit1 protein when equivalent amounts of cDNA
            were transfected.
REFERENCE   2  (bases 1 to 804)
  AUTHORS   Hua,W., Miao,S., Zou,W., Yang,H. and Chen,B.L.
  TITLE     Pathological implication and function of Bcl2-inhibitor of
            transcription in ovarian serous papillary adenocarcinomas
  JOURNAL   Neoplasma 60 (2), 143-150 (2013)
   PUBMED   23259782
  REMARK    GeneRIF: Bit1 may be a useful pathological marker and a prognostic
            marker for serous papillary adenocarcinomas outcome.
REFERENCE   3  (bases 1 to 804)
  AUTHORS   Brunquell,C., Biliran,H., Jennings,S., Ireland,S.K., Chen,R. and
            Ruoslahti,E.
  TITLE     TLE1 is an anoikis regulator and is downregulated by Bit1 in breast
            cancer cells
  JOURNAL   Mol. Cancer Res. 10 (11), 1482-1495 (2012)
   PUBMED   22952044
  REMARK    GeneRIF: TLE1 inhibits the Bit1 anoikis pathway by reducing the
            formation of the proapoptotic Bit1-AES complex in part through
            sequestration of AES in the nucleus.
REFERENCE   4  (bases 1 to 804)
  AUTHORS   Griffiths,G.S., Grundl,M., Leychenko,A., Reiter,S.,
            Young-Robbins,S.S., Sulzmaier,F.J., Caliva,M.J., Ramos,J.W. and
            Matter,M.L.
  TITLE     Bit-1 mediates integrin-dependent cell survival through activation
            of the NFkappaB pathway
  JOURNAL   J. Biol. Chem. 286 (16), 14713-14723 (2011)
   PUBMED   21383007
  REMARK    GeneRIF: Bit-1 mediates integrin-dependent cell survival through
            activation of the NFkappaB pathway
REFERENCE   5  (bases 1 to 804)
  AUTHORS   Karmali,P.P., Brunquell,C., Tram,H., Ireland,S.K., Ruoslahti,E. and
            Biliran,H.
  TITLE     Metastasis of tumor cells is enhanced by downregulation of Bit1
  JOURNAL   PLoS ONE 6 (8), E23840 (2011)
   PUBMED   21886829
  REMARK    GeneRIF: downregulation of Bit1 conferred cancer cells with
            enhanced anoikis resistance, adhesive and migratory properties in
            vitro
REFERENCE   6  (bases 1 to 804)
  AUTHORS   Fan,T., Jiang,G., Suo,Z., Liu,H., Xu,P., Ji,Z., Zhang,L. and
            Yang,G.
  TITLE     Down-regulation of the apoptosis-inducing factor or Bcl-2 inhibitor
            of transcription by RNA interference can alleviate
            TAp63gamma-induced apoptosis in esophageal squamous carcinoma
            EC9706 cells
  JOURNAL   Int. J. Oncol. 35 (2), 359-367 (2009)
   PUBMED   19578750
  REMARK    GeneRIF: TAp63gamma could induce apoptosis in human esophageal
            squamous cancer EC9706 cells, through at least releasing AIF and
            Bit1 from mitochindria into cytosol and nucleus, where apoptotic
            cascade takes place.
REFERENCE   7  (bases 1 to 804)
  AUTHORS   Biliran,H., Jan,Y., Chen,R., Pasquale,E.B. and Ruoslahti,E.
  TITLE     Protein kinase D is a positive regulator of Bit1 apoptotic function
  JOURNAL   J. Biol. Chem. 283 (42), 28029-28037 (2008)
   PUBMED   18703509
  REMARK    GeneRIF: the PKD serine/threonine kinase is one of the signaling
            molecules through which integrin-mediated cell attachment controls
            Bit1 activity and anoikis
            Erratum:[J Biol Chem. 2011 Mar 4;286(9):7714]
REFERENCE   8  (bases 1 to 804)
  AUTHORS   Bouchentouf,M., Benabdallah,B.F., Rousseau,J., Schwartz,L.M. and
            Tremblay,J.P.
  TITLE     Induction of Anoikis following myoblast transplantation into SCID
            mouse muscles requires the Bit1 and FADD pathways
  JOURNAL   Am. J. Transplant. 7 (6), 1491-1505 (2007)
   PUBMED   17511679
  REMARK    GeneRIF: Reduced expression of the proapoptotic proteins Bit1 or
            overexpression of Bcl-2 improved myoblast transplantation survival.
REFERENCE   9  (bases 1 to 804)
  AUTHORS   Jan,Y., Matter,M., Pai,J.T., Chen,Y.L., Pilch,J., Komatsu,M.,
            Ong,E., Fukuda,M. and Ruoslahti,E.
  TITLE     A mitochondrial protein, Bit1, mediates apoptosis regulated by
            integrins and Groucho/TLE corepressors
  JOURNAL   Cell 116 (5), 751-762 (2004)
   PUBMED   15006356
  REMARK    GeneRIF: Results identify Bit1, a mitochondrial protein released
            into the cytoplasm during apoptosis that forms a complex with AES,
            a small Groucho/transducin-like enhancer of split (TLE) protein
            Erratum:[Cell. 2005 Aug 12;122(3):485-6]
REFERENCE   10 (bases 1 to 804)
  AUTHORS   De Pereda,J.M., Waas,W.F., Jan,Y., Ruoslahti,E., Schimmel,P. and
            Pascual,J.
  TITLE     Crystal structure of a human peptidyl-tRNA hydrolase reveals a new
            fold and suggests basis for a bifunctional activity
  JOURNAL   J. Biol. Chem. 279 (9), 8111-8115 (2004)
   PUBMED   14660562
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF151905.1.
            On Apr 22, 2005 this sequence version replaced gi:56676387.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF151905.1, BU858401.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           ERS025084 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                AF151905.1         1-28
            29-80               AF151905.1         30-81
            81-804              AF151905.1         83-806
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q23.1"
     gene            1..804
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /note="peptidyl-tRNA hydrolase 2"
                     /db_xref="GeneID:51651"
                     /db_xref="HGNC:24265"
                     /db_xref="HPRD:12273"
                     /db_xref="MIM:608625"
     exon            1..125
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    123..125
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /note="upstream in-frame stop codon"
     exon            126..798
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /inference="alignment:Splign:1.39.8"
     CDS             126..665
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /EC_number="3.1.1.29"
                     /note="peptidyl-tRNA hydrolase 2, mitochondrial; PTH 2;
                     bcl-2 inhibitor of transcription 1"
                     /codon_start=1
                     /product="peptidyl-tRNA hydrolase 2, mitochondrial
                     precursor"
                     /protein_id="NP_057161.1"
                     /db_xref="GI:7706351"
                     /db_xref="CCDS:CCDS11618.1"
                     /db_xref="GeneID:51651"
                     /db_xref="HGNC:24265"
                     /db_xref="HPRD:12273"
                     /db_xref="MIM:608625"
                     /translation="
MPSKSLVMEYLAHPSTLGLAVGVACGMCLGWSLRVCFGMLPKSKTSKTHTDTESEASILGDSGEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLGLTVSLIQDAGRTQIAPGSQTVLGIGPGPADLIDKVTGHLKLY
"
     transit_peptide 126..311
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Mitochondrion (Potential); propagated from
                     UniProtKB/Swiss-Prot (Q9Y3E5.1)"
     misc_feature    294..296
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot
                     (Q9Y3E5.1); phosphorylation site"
     mat_peptide     312..662
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /product="Peptidyl-tRNA hydrolase 2, mitochondrial"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y3E5.1)"
     misc_feature    315..662
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /note="Peptidyl-tRNA hydrolase PTH2; Region: PTH2;
                     pfam01981"
                     /db_xref="CDD:190182"
     misc_feature    318..662
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /note="Peptidyl-tRNA hydrolase, type 2 (PTH2).
                     Peptidyl-tRNA hydrolase (PTH) activity releases tRNA from
                     the premature translation termination product
                     peptidyl-tRNA, therefore allowing the tRNA and peptide to
                     be reused in protein synthesis. PTH2 is present in...;
                     Region: PTH2; cd02430"
                     /db_xref="CDD:48405"
     misc_feature    order(360..362,381..389,396..398,405..410,417..419,
                     450..452,468..470,660..662)
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /note="dimer interface [polypeptide binding]; other site"
                     /db_xref="CDD:48405"
     misc_feature    order(366..368,378..380,387..389,468..470,594..596)
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /note="putative active site [active]"
                     /db_xref="CDD:48405"
     variation       165
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /replace="a"
                     /replace="c"
                     /db_xref="dbSNP:11547273"
     STS             375..537
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /standard_name="RH93739"
                     /db_xref="UniSTS:89968"
     STS             397..694
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /standard_name="WI-20523"
                     /db_xref="UniSTS:52060"
     STS             566..707
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
                     /standard_name="D17S2149"
                     /db_xref="UniSTS:860"
     polyA_signal    775..780
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
     polyA_site      798
                     /gene="PTRH2"
                     /gene_synonym="BIT1; CGI-147; PTH2"
ORIGIN      
tcgctttgtgattcttgatccggaacttgtcacccaggaaccccggaagaggtagctcacgcgatagaaacgtgttcgctgcccagaagaagggaaggcgcgagtgaggaaaggaggtactgtagatgccctccaaatccttggttatggaatatttggctcatcccagtacactcggcttggctgttggagttgcttgtggcatgtgcctgggctggagccttcgagtatgctttgggatgctccccaaaagcaagacgagcaagacacacacagatactgaaagtgaagcaagcatcttgggagacagcggggagtacaagatgattcttgtggttcgaaatgacttaaagatgggaaaagggaaagtggctgcccagtgctctcatgctgctgtttcagcctacaagcagattcaaagaagaaatcctgaaatgctcaaacaatgggaatactgtggccagcccaaggtggtggtcaaagctcctgatgaagaaaccctgattgcattattggcccatgcaaaaatgctgggactgactgtaagtttaattcaagatgctggacgtactcagattgcaccaggctctcaaactgtcctagggattgggccaggaccagcagacctaattgacaaagtcactggtcacctaaaactttactaggtggactttgatatgacaacaacccctccatcacaagtgtttgaagcctgtcagattctaacaacaaaagctgaatttcttcacccaacttaaatgttcttgagatgaaaataaaacctattcccatgttctaaaaaaa
//

Annotations:

ANNOTATIONS from NCBI Entrez Gene (20130726):
            GeneID:51651 -> Molecular function: GO:0004045 [aminoacyl-tRNA hydrolase activity] evidence: IEA
            GeneID:51651 -> Molecular function: GO:0005515 [protein binding] evidence: IPI
            GeneID:51651 -> Biological process: GO:0006915 [apoptotic process] evidence: IEA
            GeneID:51651 -> Biological process: GO:0010629 [negative regulation of gene expression] evidence: IMP
            GeneID:51651 -> Biological process: GO:2000210 [positive regulation of anoikis] evidence: IMP
            GeneID:51651 -> Biological process: GO:2000811 [negative regulation of anoikis] evidence: IMP
            GeneID:51651 -> Cellular component: GO:0005739 [mitochondrion] evidence: IDA
            GeneID:51651 -> Cellular component: GO:0005739 [mitochondrion] evidence: IMP
            GeneID:51651 -> Cellular component: GO:0005829 [cytosol] evidence: IMP
ANNOTATIONS from NCBI Entrez Gene (20130726):
            NP_057161 -> EC 3.1.1.29

by @meso_cacase at DBCLS
This page is licensed under a Creative Commons Attribution 2.1 Japan License.