LOCUS       NM_170695               2264 bp    mRNA    linear   PRI 18-JUL-2013
DEFINITION  Homo sapiens TGFB-induced factor homeobox 1 (TGIF1), transcript
            variant 1, mRNA.
ACCESSION   NM_170695
VERSION     NM_170695.3  GI:522838230
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Valton,J., Dupuy,A., Daboussi,F., Thomas,S., Marechal,A.,
            Macmaster,R., Melliand,K., Juillerat,A. and Duchateau,P.
  TITLE     Overcoming transcription activator-like effector (TALE) DNA binding
            domain sensitivity to cytosine methylation
  JOURNAL   J. Biol. Chem. 287 (46), 38427-38432 (2012)
   PUBMED   23019344
  REMARK    GeneRIF: biochemical analysis of how to overcome TALE DNA binding
            domain sensitivity to cytosine methylation
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Hneino,M., Blirando,K., Buard,V., Tarlet,G., Benderitter,M.,
            Hoodless,P., Francois,A. and Milliat,F.
  TITLE     The TG-interacting factor TGIF1 regulates stress-induced
            proinflammatory phenotype of endothelial cells
  JOURNAL   J. Biol. Chem. 287 (46), 38913-38921 (2012)
   PUBMED   22995913
  REMARK    GeneRIF: TGIF1 plays a role in TNF-alpha- and radiation-induced
            inflammation and it could be a target in limiting this event in the
            vascular compartment
REFERENCE   3  (bases 1 to 2264)
  AUTHORS   Yeh,B.W., Wu,W.J., Li,W.M., Li,C.C., Huang,C.N., Kang,W.Y.,
            Liu,Z.M. and Huang,H.S.
  TITLE     Overexpression of TG-interacting factor is associated with worse
            prognosis in upper urinary tract urothelial carcinoma
  JOURNAL   Am. J. Pathol. 181 (3), 1044-1055 (2012)
   PUBMED   22771156
  REMARK    GeneRIF: TGIF contributes to the progression of urothelial
            carcinoma via the phosphatidylinositol 3-kinase-AKT pathway.
REFERENCE   4  (bases 1 to 2264)
  AUTHORS   Huang,H.S., Liu,Z.M., Chen,P.C., Tseng,H.Y. and Yeh,B.W.
  TITLE     TG-interacting factor-induced superoxide production from NADPH
            oxidase contributes to the migration/invasion of urothelial
            carcinoma
  JOURNAL   Free Radic. Biol. Med. 53 (4), 769-778 (2012)
   PUBMED   22728270
  REMARK    GeneRIF: TGIF can promote cellular migration/invasion activity of
            urothelial carcinoma cells.
REFERENCE   5  (bases 1 to 2264)
  AUTHORS   Ribeiro-Bicudo,L.A., Quiezi,R.G., Guion-Almeida,M.L., Legnaro,C.
            and Richieri-Costa,A.
  TITLE     Exclusion of mutations in TGIF, ALX3, and ALX4 genes in patients
            with the syndrome of frontonasal dysgenesis, callosal agenesis,
            basal encephalocele, and eye anomalies
  JOURNAL   Am. J. Med. Genet. A 158A (5), 1233-1235 (2012)
   PUBMED   22496059
  REMARK    GeneRIF: Exclusion of mutations in TGIF gene in patients with the
            syndrome of frontonasal dysgenesis, callosal agenesis, basal
            encephalocele, and eye anomalies
REFERENCE   6  (bases 1 to 2264)
  AUTHORS   Hamid,R., Patterson,J. and Brandt,S.J.
  TITLE     Genomic structure, alternative splicing and expression of
            TG-interacting factor, in human myeloid leukemia blasts and cell
            lines
  JOURNAL   Biochim. Biophys. Acta 1779 (5), 347-355 (2008)
   PUBMED   18455519
  REMARK    GeneRIF: A detailed description of the TGIF locus characterizing 12
            TGIF splice isoforms.
REFERENCE   7  (bases 1 to 2264)
  AUTHORS   Yang,Y., Hwang,C.K., D'Souza,U.M., Lee,S.H., Junn,E. and
            Mouradian,M.M.
  TITLE     Three-amino acid extension loop homeodomain proteins Meis2 and TGIF
            differentially regulate transcription
  JOURNAL   J. Biol. Chem. 275 (27), 20734-20741 (2000)
   PUBMED   10764806
REFERENCE   8  (bases 1 to 2264)
  AUTHORS   Wotton,D., Lo,R.S., Lee,S. and Massague,J.
  TITLE     A Smad transcriptional corepressor
  JOURNAL   Cell 97 (1), 29-39 (1999)
   PUBMED   10199400
REFERENCE   9  (bases 1 to 2264)
  AUTHORS   Bertolino,E., Reimund,B., Wildt-Perinic,D. and Clerc,R.G.
  TITLE     A novel homeobox protein which recognizes a TGT core and
            functionally interferes with a retinoid-responsive motif
  JOURNAL   J. Biol. Chem. 270 (52), 31178-31188 (1995)
   PUBMED   8537382
REFERENCE   10 (bases 1 to 2264)
  AUTHORS   Overhauser,J., Mitchell,H.F., Zackai,E.H., Tick,D.B., Rojas,K. and
            Muenke,M.
  TITLE     Physical mapping of the holoprosencephaly critical region in
            18p11.3
  JOURNAL   Am. J. Hum. Genet. 57 (5), 1080-1085 (1995)
   PUBMED   7485158
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP001025.6 and BC031268.1.
            This sequence is a reference standard in the RefSeqGene project.
            On Jul 10, 2013 this sequence version replaced gi:28178842.
            
            Summary: The protein encoded by this gene is a member of the
            three-amino acid loop extension (TALE) superclass of atypical
            homeodomains. TALE homeobox proteins are highly conserved
            transcription regulators. This particular homeodomain binds to a
            previously characterized retinoid X receptor responsive element
            from the cellular retinol-binding protein II promoter. In addition
            to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha
            transcription activation of the retinoic acid responsive element,
            the protein is an active transcriptional co-repressor of SMAD2 and
            may participate in the transmission of nuclear signals during
            development and in the adult. Mutations in this gene are associated
            with holoprosencephaly type 4, which is a structural anomaly of the
            brain. Alternative splicing has been observed at this locus and
            multiple splice variants encoding distinct isoforms are described.
            [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) is the longest transcript and
            encodes the longest isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031268.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           ERS025084, ERS025088 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-862               AP001025.6         187303-188164
            863-2264            BC031268.1         591-1992
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.3"
     gene            1..2264
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /note="TGFB-induced factor homeobox 1"
                     /db_xref="GeneID:7050"
                     /db_xref="HGNC:11776"
                     /db_xref="MIM:602630"
     exon            1..978
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    510..512
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /note="upstream in-frame stop codon"
     CDS             576..1781
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /note="isoform a is encoded by transcript variant 1;
                     homeobox protein TGIF1; TALE homeobox TG-interacting
                     factor; transforming growth factor-beta-induced factor;
                     5'-TG-3'-interacting factor 1"
                     /codon_start=1
                     /product="homeobox protein TGIF1 isoform a"
                     /protein_id="NP_733796.2"
                     /db_xref="GI:28178843"
                     /db_xref="CCDS:CCDS11834.1"
                     /db_xref="GeneID:7050"
                     /db_xref="HGNC:11776"
                     /db_xref="MIM:602630"
                     /translation="

MVLAQSRVSAGVGSPHCSGSGGGGSDSFPWPASHPGNPQCSFSTAFLASPRLSRGTLAYLPPAPWSSLATPSALLGSSCAPPPPPARCPQPRALSPELGTKAGPRRPHRWELPRSPSQGAQGPAPRRRLLETMKGIVAASGSETEDEDSMDIPLDLSSSAGSGKRRRRGNLPKESVQILRDWLYEHRYNAYPSEQEKALLSQQTHLSTLQVCNWFINARRRLLPDMLRKDGKDPNQFTISRRGAKISETSSVESVMGIKNFMPALEETPFHSCTAGPNPTLGRPLSPKPSSPGSVLARPSVICHTTVTALKDVPFSLCQSVGVGQNTDIQQIAAKNFTDTSLMYPEDTCKSGPSTNTQSGLFNTPPPTPPDLNQDFSGFQLLVDVALKRAAEMELQAKLTA

"
     misc_feature    1032..1046
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (Q15583.3);
                     Region: CTBP-binding motif"
     misc_feature    1083..1241
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /note="Homeodomain;  DNA binding domains involved in the
                     transcriptional regulation of key eukaryotic developmental
                     processes; may bind to DNA as monomers or as homo- and/or
                     heterodimers, in a sequence-specific manner; Region:
                     homeodomain; cd00086"
                     /db_xref="CDD:28970"
     misc_feature    order(1083..1085,1212..1214,1221..1226,1233..1235)
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /note="specific DNA base contacts [nucleotide binding];
                     other site"
                     /db_xref="CDD:28970"
     misc_feature    order(1086..1088,1146..1148,1164..1166,1203..1205,
                     1209..1214,1221..1226,1230..1238)
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:28970"
     exon            979..1205
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /inference="alignment:Splign:1.39.8"
     exon            1206..2252
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /inference="alignment:Splign:1.39.8"
     STS             1803..2050
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /standard_name="STS-X89750"
                     /db_xref="UniSTS:67859"
     STS             1879..2139
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /standard_name="RH39275"
                     /db_xref="UniSTS:88834"
     STS             1904..2136
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
                     /standard_name="RH76383"
                     /db_xref="UniSTS:90886"
     polyA_signal    2231..2236
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
     polyA_site      2252
                     /gene="TGIF1"
                     /gene_synonym="HPE4; TGIF"
ORIGIN      

cttttgaagttaacaaaaattgagtccctggctgggaggtcccccgagtgttccgctgtgggctggaggtggcgtttctgtcgtgatttatgtggagtggttcaaaacagaagttaatcactcgggaagcggacgggaggggcggcgctactgcgcatgcccgggagccgcctggagtttggaactccacattctttcagacccggcccgctgcggggcgttcctggggggtagcctcaaggccagcggggttccttcggctgcgtttctgtgggaggccctgaaacgcgcggagcttccctctgcctccaggctttcccagcgagagtgaaattaaacttgaaactcggatcaactggcagtcgttgttggtagaacgccctaaggacccctccccgcgggacggagggaggactcgggacagggaattggccctgggagaaaacgcgcggggggcgtccgagacgccccgtgaaagccgtgccgacccttgggaggactgacaggtctagagacacgcgctgtctgttgtggtgggcctcccgggaataagtgaggggctctgtgtttcgaggatggttctagcgcagagccgggtgtctgccggggtgggctccccgcattgttcgggctccggcgggggcggctctgattcctttccatggcccgcctcccaccccgggaatccccagtgctccttttccacggcttttctggcgtccccccgactctcccgcggcactttggcctaccttcccccagcgccgtggtcctccctggcgaccccctctgcgctcctggggtcctcctgcgccccccctcctccaccggcgcgctgcccacagccgcgtgccctctccccggagctggggaccaaggctgggccccgccggccgcatcggtgggaacttccgcggtccccatcccagggcgcacagggtccagctcctcggcgccgactcctggaaacaatgaaaggtattgttgcagcatctggcagtgagactgaggatgaggacagcatggacattcccttggacctttcttcatccgctggctcaggcaagagaaggagaaggggcaacctacccaaggagtctgtgcagattcttcgggattggctgtatgagcaccgttacaatgcctatccttcagagcaagaaaaagcgttgctgtcccagcaaacacacctgtctacgctacaggtctgtaactggttcatcaacgcccgccgcaggctcctccctgacatgctgagaaaggatggcaaagatccaaatcagttcacaatttcccgccgtggggccaagatttctgaaacgagctctgtggagtccgtgatgggcatcaaaaacttcatgccagctctagaggagaccccatttcattcctgtacagctgggccaaacccaaccctagggaggccactgtctcctaagccgtcatccccgggatcagttttggctcgtccatcagtgatctgccataccactgtgactgcattgaaagatgtccctttctctctctgccagtcggtcggtgtgggacaaaacacagatatacagcagatagcggccaaaaacttcacagacacctctctcatgtacccagaggacacttgtaaatctggaccaagtacgaatacacagagtggtcttttcaacactcctccccctactccaccggacctcaaccaggacttcagtggatttcagcttctagtggatgttgcactcaaacgggctgcagagatggagcttcaggcaaaacttacagcttaacccattttcaagcaaaacagttctcagaaatgtcatgattgccggggtgaaggcaagagatgaattgcattattttatatattttttattaatatttgcacatgggattgctaaaacagcttcctgttactgagatgtcttcaatggaatacagtcattccaagaactataaacttaaagctactgtagaaacaaagggttttcttttttaaatgtttcttggtagattattcataatgtgagatggttcccaatatcatgtgattttttttttcctccccttccctttttttgttattttttcagactgtgcaatacttagagaacctatagcatcttctcattcccatgtggaacaggatgcccacatactgtctaattaataaattttccattttttttcaaacaagtatgaatctagttggttgatgccttttttttcatgacataataaagtattttctttaaaaaaaaaaaaaaaaa

//

ANNOTATIONS from NCBI Entrez Gene (20130726):
            GeneID:7050 -> Molecular function: GO:0003682 [chromatin binding] evidence: IEA
            GeneID:7050 -> Molecular function: GO:0003700 [sequence-specific DNA binding transcription factor activity] evidence: IEA
            GeneID:7050 -> Molecular function: GO:0003714 [transcription corepressor activity] evidence: TAS
            GeneID:7050 -> Molecular function: GO:0043565 [sequence-specific DNA binding] evidence: IEA
            GeneID:7050 -> Molecular function: GO:0070410 [co-SMAD binding] evidence: IEA
            GeneID:7050 -> Biological process: GO:0000122 [negative regulation of transcription from RNA polymerase II promoter] evidence: TAS
            GeneID:7050 -> Biological process: GO:0001843 [neural tube closure] evidence: IEA
            GeneID:7050 -> Biological process: GO:0006351 [transcription, DNA-dependent] evidence: TAS
            GeneID:7050 -> Biological process: GO:0006367 [transcription initiation from RNA polymerase II promoter] evidence: TAS
            GeneID:7050 -> Biological process: GO:0007179 [transforming growth factor beta receptor signaling pathway] evidence: TAS
            GeneID:7050 -> Biological process: GO:0007275 [multicellular organismal development] evidence: TAS
            GeneID:7050 -> Biological process: GO:0007368 [determination of left/right symmetry] evidence: IEA
            GeneID:7050 -> Biological process: GO:0008285 [negative regulation of cell proliferation] evidence: IEA
            GeneID:7050 -> Biological process: GO:0009953 [dorsal/ventral pattern formation] evidence: IEA
            GeneID:7050 -> Biological process: GO:0010467 [gene expression] evidence: TAS
            GeneID:7050 -> Biological process: GO:0010470 [regulation of gastrulation] evidence: IEA
            GeneID:7050 -> Biological process: GO:0038092 [nodal signaling pathway] evidence: IEA
            GeneID:7050 -> Biological process: GO:0042493 [response to drug] evidence: IEA
            GeneID:7050 -> Biological process: GO:0045666 [positive regulation of neuron differentiation] evidence: IEA
            GeneID:7050 -> Biological process: GO:0048146 [positive regulation of fibroblast proliferation] evidence: IEA
            GeneID:7050 -> Biological process: GO:0048387 [negative regulation of retinoic acid receptor signaling pathway] evidence: IEA
            GeneID:7050 -> Biological process: GO:0060041 [retina development in camera-type eye] evidence: IEA
            GeneID:7050 -> Cellular component: GO:0005654 [nucleoplasm] evidence: TAS