LOCUS       NM_006871               1940 bp    mRNA    linear   PRI 08-JUN-2013
DEFINITION  Homo sapiens receptor-interacting serine-threonine kinase 3
            (RIPK3), mRNA.
ACCESSION   NM_006871
VERSION     NM_006871.3  GI:93141035
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Kikuchi,M., Kuroki,S., Kayama,M., Sakaguchi,S., Lee,K.K. and
            Yonehara,S.
  TITLE     Protease activity of procaspase-8 is essential for cell survival by
            inhibiting both apoptotic and nonapoptotic cell death dependent on
            receptor-interacting protein kinase 1 (RIP1) and RIP3
  JOURNAL   J. Biol. Chem. 287 (49), 41165-41173 (2012)
   PUBMED   23071110
  REMARK    GeneRIF: procaspase-8 activity is essential for cell survival by
            inhibiting both apoptotic and nonapoptotic cell death dependent on
            receptor-interacting protein kinase 1 (RIP1) and RIP3
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Li,J., McQuade,T., Siemer,A.B., Napetschnig,J., Moriwaki,K.,
            Hsiao,Y.S., Damko,E., Moquin,D., Walz,T., McDermott,A., Chan,F.K.
            and Wu,H.
  TITLE     The RIP1/RIP3 necrosome forms a functional amyloid signaling
            complex required for programmed necrosis
  JOURNAL   Cell 150 (2), 339-350 (2012)
   PUBMED   22817896
  REMARK    GeneRIF: Study shows that RIP1 and RIP3 form an amyloid structure
            through their RIP homotypic interaction motifs and that this
            heterodimeric amyloid structure is a functional signaling complex
            that mediates programmed necrosis.
REFERENCE   3  (bases 1 to 1940)
  AUTHORS   Zhao,J., Jitkaew,S., Cai,Z., Choksi,S., Li,Q., Luo,J. and Liu,Z.G.
  TITLE     Mixed lineage kinase domain-like is a key receptor interacting
            protein 3 downstream component of TNF-induced necrosis
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 109 (14), 5322-5327 (2012)
   PUBMED   22421439
  REMARK    GeneRIF: study suggests that MLKL is a key RIP3 downstream
            component of TNF-induced necrotic cell death
REFERENCE   4  (bases 1 to 1940)
  AUTHORS   Galluzzi,L., Kepp,O. and Kroemer,G.
  TITLE     FADD: an endogenous inhibitor of RIP3-driven regulated necrosis
  JOURNAL   Cell Res. 21 (10), 1383-1385 (2011)
   PUBMED   21894190
  REMARK    GeneRIF: FADD: an endogenous inhibitor of RIP3-driven regulated
            necrosis
            Review article
REFERENCE   5  (bases 1 to 1940)
  AUTHORS   Cho,Y., Challa,S. and Chan,F.K.
  TITLE     A RNA interference screen identifies RIP3 as an essential inducer
            of TNF-induced programmed necrosis
  JOURNAL   Adv. Exp. Med. Biol. 691, 589-593 (2011)
   PUBMED   21153365
  REMARK    GeneRIF: RIP3 is an essential inducer of TNF-induced programmed
            necrosis
REFERENCE   6  (bases 1 to 1940)
  AUTHORS   Meylan,E., Burns,K., Hofmann,K., Blancheteau,V., Martinon,F.,
            Kelliher,M. and Tschopp,J.
  TITLE     RIP1 is an essential mediator of Toll-like receptor 3-induced
            NF-kappa B activation
  JOURNAL   Nat. Immunol. 5 (5), 503-507 (2004)
   PUBMED   15064760
REFERENCE   7  (bases 1 to 1940)
  AUTHORS   Sun,X., Yin,J., Starovasnik,M.A., Fairbrother,W.J. and Dixit,V.M.
  TITLE     Identification of a novel homotypic interaction motif required for
            the phosphorylation of receptor-interacting protein (RIP) by RIP3
  JOURNAL   J. Biol. Chem. 277 (11), 9505-9511 (2002)
   PUBMED   11734559
REFERENCE   8  (bases 1 to 1940)
  AUTHORS   Kasof,G.M., Prosser,J.C., Liu,D., Lorenzi,M.V. and Gomes,B.C.
  TITLE     The RIP-like kinase, RIP3, induces apoptosis and NF-kappaB nuclear
            translocation and localizes to mitochondria
  JOURNAL   FEBS Lett. 473 (3), 285-291 (2000)
   PUBMED   10818227
REFERENCE   9  (bases 1 to 1940)
  AUTHORS   Sun,X., Lee,J., Navas,T., Baldwin,D.T., Stewart,T.A. and Dixit,V.M.
  TITLE     RIP3, a novel apoptosis-inducing kinase
  JOURNAL   J. Biol. Chem. 274 (24), 16871-16875 (1999)
   PUBMED   10358032
REFERENCE   10 (bases 1 to 1940)
  AUTHORS   Yu,P.W., Huang,B.C., Shen,M., Quast,J., Chan,E., Xu,X., Nolan,G.P.,
            Payan,D.G. and Luo,Y.
  TITLE     Identification of RIP3, a RIP-like kinase that activates apoptosis
            and NFkappaB
  JOURNAL   Curr. Biol. 9 (10), 539-542 (1999)
   PUBMED   10339433
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB274842.1, BC062584.1 and
            BE676675.1.
            On Apr 21, 2006 this sequence version replaced gi:40254843.
            
            Summary: The product of this gene is a member of the
            receptor-interacting protein (RIP) family of serine/threonine
            protein kinases, and contains a C-terminal domain unique from other
            RIP family members. The encoded protein is predominantly localized
            to the cytoplasm, and can undergo nucleocytoplasmic shuttling
            dependent on novel nuclear localization and export signals. It is a
            component of the tumor necrosis factor (TNF) receptor-I signaling
            complex, and can induce apoptosis and weakly activate the NF-kappaB
            transcription factor. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062584.1, AK075275.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           ERS025098 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-69                DB274842.1         1-69
            70-1852             BC062584.1         1-1783
            1853-1940           BE676675.1         1-88                c
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q11.2"
     gene            1..1940
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /note="receptor-interacting serine-threonine kinase 3"
                     /db_xref="GeneID:11035"
                     /db_xref="HGNC:10021"
                     /db_xref="HPRD:09315"
                     /db_xref="MIM:605817"
     exon            1..230
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /inference="alignment:Splign:1.39.8"
     variation       52
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /replace="a"
                     /replace="g"
                     /db_xref="dbSNP:3212250"
     variation       58
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /replace="a"
                     /replace="g"
                     /db_xref="dbSNP:3212251"
     misc_feature    97..99
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /note="upstream in-frame stop codon"
     CDS             211..1767
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /EC_number="2.7.11.1"
                     /note="receptor interacting protein 3; RIP-like protein
                     kinase 3; RIP-3; receptor-interacting protein 3"
                     /codon_start=1
                     /product="receptor-interacting serine/threonine-protein
                     kinase 3"
                     /protein_id="NP_006862.2"
                     /db_xref="GI:40254844"
                     /db_xref="CCDS:CCDS9628.1"
                     /db_xref="GeneID:11035"
                     /db_xref="HGNC:10021"
                     /db_xref="HPRD:09315"
                     /db_xref="MIM:605817"
                     /translation="

MSCVKLWPSGAPAPLVSIEELENQELVGKGGFGTVFRAQHRKWGYDVAVKIVNSKAISREVKAMASLDNEFVLRLEGVIEKVNWDQDPKPALVTKFMENGSLSGLLQSQCPRPWPLLCRLLKEVVLGMFYLHDQNPVLLHRDLKPSNVLLDPELHVKLADFGLSTFQGGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTASDVYSFGILMWAVLAGREVELPTEPSLVYEAVCNRQNRPSLAELPQAGPETPGLEGLKELMQLCWSSEPKDRPSFQECLPKTDEVFQMVENNMNAAVSTVKDFLSQLRSSNRRFSIPESGQGGTEMDGFRRTIENQHSRNDVMVSEWLNKLNLEEPPSSVPKKCPSLTKRSRAQEEQVPQAWTAGTSSDSMAQPPQTPETSTFRNQMPSPTSTGTPSPGPRGNQGAERQGMNWSCRTPEPNPVTGRPLVNIYNCSGVQVGDNNYLTMQQTTALPTWGLAPSGKGRGLQHPPPVGSQEGPKDPEAWSRPQGWYNHSGK

"
     misc_feature    274..1050
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /note="Serine/Threonine protein kinases, catalytic domain;
                     Region: S_TKc; smart00220"
                     /db_xref="CDD:197582"
     misc_feature    289..1050
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /note="Catalytic domain of Protein Kinases; Region: PKc;
                     cd00180"
                     /db_xref="CDD:173623"
     misc_feature    order(289..303,313..315,352..354,358..360,427..429,
                     490..501,511..513,517..519,634..636,640..642,646..651,
                     655..657,688..690,703..705,751..762)
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /note="active site"
                     /db_xref="CDD:173623"
     misc_feature    order(289..303,313..315,352..354,358..360,427..429,
                     490..501,511..513,634..636,640..642,646..651,655..657,
                     688..690)
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:173623"
     misc_feature    order(301..303,511..513,517..519,634..636,640..642,
                     646..648,703..705,751..762)
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /note="substrate binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:173623"
     misc_feature    order(685..690,697..711,751..762)
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /note="activation loop (A-loop); other site"
                     /db_xref="CDD:173623"
     misc_feature    805..807
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine, by autocatalysis; propagated from
                     UniProtKB/Swiss-Prot (Q9Y572.2); phosphorylation site"
     misc_feature    1480..1617
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /note="RIP homotypic interaction motif; Region: RHIM;
                     pfam12721"
                     /db_xref="CDD:205042"
     misc_feature    1558..1608
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y572.2);
                     Region: RIP homotypic interaction motif (RHIM)"
     exon            231..371
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /inference="alignment:Splign:1.39.8"
     exon            372..681
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /inference="alignment:Splign:1.39.8"
     variation       514
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /replace="c"
                     /replace="t"
                     /db_xref="dbSNP:3181247"
     exon            682..827
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /inference="alignment:Splign:1.39.8"
     exon            828..874
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /inference="alignment:Splign:1.39.8"
     exon            875..1042
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /inference="alignment:Splign:1.39.8"
     exon            1043..1110
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /inference="alignment:Splign:1.39.8"
     exon            1111..1485
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /inference="alignment:Splign:1.39.8"
     exon            1486..1546
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /inference="alignment:Splign:1.39.8"
     exon            1547..1921
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /inference="alignment:Splign:1.39.8"
     variation       1656
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /replace="a"
                     /replace="g"
                     /db_xref="dbSNP:2295299"
     variation       1685
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /replace="a"
                     /replace="c"
                     /db_xref="dbSNP:3212254"
     STS             1780..1905
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
                     /standard_name="RH91896"
                     /db_xref="UniSTS:85933"
     polyA_signal    1897..1902
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
     polyA_site      1921
                     /gene="RIPK3"
                     /gene_synonym="RIP3"
ORIGIN      

gcgggactgtagaggcgcctataagggaagttgttcagtcaactcggaaaaagggtaacaacccggaaagtagactcaccgtcttggtctagagactgacccctgcacagacagaccccttcccctctctgcgaaaggaccaagccccagaagtcactccatctcctacggctcgcaatttccagaggccccctggcaccttccagcctgatgtcgtgcgtcaagttatggcccagcggtgcccccgcccccttggtgtccatcgaggaactggagaaccaggagctcgtcggcaaaggcgggttcggcacagtgttccgggcgcaacataggaagtggggctacgatgtggcggtcaagatcgtaaactcgaaggcgatatccagggaggtcaaggccatggcaagtctggataacgaattcgtgctgcgcctagaaggggttatcgagaaggtgaactgggaccaagatcccaagccggctctggtgactaaattcatggagaacggctccttgtcggggctgctgcagtcccagtgccctcggccctggccgctcctttgccgcctgctgaaagaagtggtgcttgggatgttttacctgcacgaccagaacccggtgctcctgcaccgggacctcaagccatccaacgtcctgctggacccagagctgcacgtcaagctggcagattttggcctgtccacatttcagggaggctcacagtcagggacagggtccggggagccagggggcaccctgggctacttggccccagaactgtttgttaacgtaaaccggaaggcctccacagccagtgacgtctacagcttcgggatcctaatgtgggcagtgcttgctggaagagaagttgagttgccaaccgaaccatcactcgtgtacgaagcagtgtgcaacaggcagaaccggccttcattggctgagctgccccaagccgggcctgagactcccggcttagaaggactgaaggagctaatgcagctctgctggagcagtgagcccaaggacagaccctccttccaggaatgcctaccaaaaactgatgaagtcttccagatggtggagaacaatatgaatgctgctgtctccacggtaaaggatttcctgtctcagctcaggagcagcaataggagattttctatcccagagtcaggccaaggagggacagaaatggatggctttaggagaaccatagaaaaccagcactctcgtaatgatgtcatggtttctgagtggctaaacaaactgaatctagaggagcctcccagctctgttcctaaaaaatgcccgagccttaccaagaggagcagggcacaagaggagcaggttccacaagcctggacagcaggcacatcttcagattcgatggcccaacctccccagactccagagacctcaactttcagaaaccagatgcccagccctacctcaactggaacaccaagtcctggaccccgagggaatcagggggctgagagacaaggcatgaactggtcctgcaggaccccggagccaaatccagtaacagggcgaccgctcgttaacatatacaactgctctggggtgcaagttggagacaacaactacttgactatgcaacagacaactgccttgcccacatggggcttggcaccttcgggcaaggggaggggcttgcagcaccccccaccagtaggttcgcaagaaggccctaaagatcctgaagcctggagcaggccacagggttggtataatcatagcgggaaataaagcaccttccaagcttgcctccaagagttacgagttaaggaagagtgccaccccttgaggcccctgacttccttctagggcagtctggcctgcccacaaactgactttgtgacctgtcccccaggagtcaataaacatgatggaatgctagtcaaaaaaaaaaaaaaaaaaaa

//

ANNOTATIONS from NCBI Entrez Gene (20130726):
            GeneID:11035 -> Molecular function: GO:0003713 [transcription coactivator activity] evidence: TAS
            GeneID:11035 -> Molecular function: GO:0004672 [protein kinase activity] evidence: TAS
            GeneID:11035 -> Molecular function: GO:0004674 [protein serine/threonine kinase activity] evidence: TAS
            GeneID:11035 -> Molecular function: GO:0004704 [NF-kappaB-inducing kinase activity] evidence: IEA
            GeneID:11035 -> Molecular function: GO:0005515 [protein binding] evidence: IPI
            GeneID:11035 -> Molecular function: GO:0005524 [ATP binding] evidence: IEA
            GeneID:11035 -> Molecular function: GO:0042802 [identical protein binding] evidence: IPI
            GeneID:11035 -> Biological process: GO:0002224 [toll-like receptor signaling pathway] evidence: TAS
            GeneID:11035 -> Biological process: GO:0002756 [MyD88-independent toll-like receptor signaling pathway] evidence: TAS
            GeneID:11035 -> Biological process: GO:0006464 [cellular protein modification process] evidence: TAS
            GeneID:11035 -> Biological process: GO:0006917 [induction of apoptosis] evidence: IEA
            GeneID:11035 -> Biological process: GO:0007165 [signal transduction] evidence: TAS
            GeneID:11035 -> Biological process: GO:0007249 [I-kappaB kinase/NF-kappaB cascade] evidence: IEA
            GeneID:11035 -> Biological process: GO:0032147 [activation of protein kinase activity] evidence: IMP
            GeneID:11035 -> Biological process: GO:0032481 [positive regulation of type I interferon production] evidence: TAS
            GeneID:11035 -> Biological process: GO:0034138 [toll-like receptor 3 signaling pathway] evidence: TAS
            GeneID:11035 -> Biological process: GO:0034142 [toll-like receptor 4 signaling pathway] evidence: TAS
            GeneID:11035 -> Biological process: GO:0035666 [TRIF-dependent toll-like receptor signaling pathway] evidence: TAS
            GeneID:11035 -> Biological process: GO:0045087 [innate immune response] evidence: TAS
            GeneID:11035 -> Biological process: GO:0051092 [positive regulation of NF-kappaB transcription factor activity] evidence: IDA
            GeneID:11035 -> Biological process: GO:0051260 [protein homooligomerization] evidence: IDA
            GeneID:11035 -> Biological process: GO:0051291 [protein heterooligomerization] evidence: IDA
            GeneID:11035 -> Biological process: GO:0060545 [positive regulation of necroptosis] evidence: IMP
            GeneID:11035 -> Biological process: GO:0097190 [apoptotic signaling pathway] evidence: TAS
            GeneID:11035 -> Cellular component: GO:0005739 [mitochondrion] evidence: IEA
            GeneID:11035 -> Cellular component: GO:0005829 [cytosol] evidence: TAS
            GeneID:11035 -> Cellular component: GO:0005886 [plasma membrane] evidence: IEA
ANNOTATIONS from NCBI Entrez Gene (20130726):
            NP_006862 -> EC 2.7.11.1