LOCUS       NM_001181663            1464 bp    mRNA    linear   PLN 17-DEC-2024
DEFINITION  Saccharomyces cerevisiae S288C DNA-directed DNA polymerase delta
            subunit POL31 (POL31), partial mRNA.
ACCESSION   NM_001181663
VERSION     NM_001181663.1
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   3  (bases 1 to 1464)
  AUTHORS   Galibert,F., Alexandraki,D., Baur,A., Boles,E., Chalwatzis,N.,
            Chuat,J.C., Coster,F., Cziepluch,C., De Haan,M., Domdey,H.,
            Durand,P., Entian,K.D., Gatius,M., Goffeau,A., Grivell,L.A.,
            Hennemann,A., Herbert,C.J., Heumann,K., Hilger,F., Hollenberg,C.P.,
            Huang,M.E., Jacq,C., Jauniaux,J.C., Katsoulou,C.,
            Karpfinger-Hartl,L. et al.
  TITLE     Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
            X
  JOURNAL   EMBO J. 15 (9), 2031-2049 (1996)
   PUBMED   8641269
REFERENCE   4  (bases 1 to 1464)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 1464)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 1464)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   7  (bases 1 to 1464)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001142).
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="X"
     gene            <1..>1464
                     /gene="POL31"
                     /locus_tag="YJR006W"
                     /gene_synonym="HUS2; HYS2; SDP5"
                     /db_xref="GeneID:853462"
     CDS             1..1464
                     /gene="POL31"
                     /locus_tag="YJR006W"
                     /gene_synonym="HUS2; HYS2; SDP5"
                     /EC_number="2.7.7.7"
                     /experiment="EXISTENCE:direct assay:GO:0003887
                     DNA-directed DNA polymerase activity [PMID:18635534]"
                     /experiment="EXISTENCE:direct assay:GO:0005634 nucleus
                     [PMID:22932476]"
                     /experiment="EXISTENCE:direct assay:GO:0005829 cytosol
                     [PMID:22932476]"
                     /experiment="EXISTENCE:direct assay:GO:0006278
                     RNA-templated DNA biosynthetic process [PMID:17429354]"
                     /experiment="EXISTENCE:direct assay:GO:0016035 zeta DNA
                     polymerase complex [PMID:22711820|PMID:23066099]"
                     /experiment="EXISTENCE:direct assay:GO:0043137 DNA
                     replication, removal of RNA primer [PMID:16837458]"
                     /note="Subunit of DNA polymerase delta (polymerase III);
                     essential for cell viability; involved in DNA replication
                     and DNA repair; forms a complex with Rev3p, Rev7p and
                     Pol32p; relocalizes to the cytosol in response to hypoxia"
                     /codon_start=1
                     /product="DNA-directed DNA polymerase delta subunit POL31"
                     /protein_id="NP_012539.1"
                     /db_xref="GeneID:853462"
                     /db_xref="SGD:S000003766"
                     /translation="

MDALLTKFNEDRSLQDENLSQPRTRVRIVDDNLYNKSNPFQLCYKKRDYGSQYYHIYQYRLKTFRERVLKECDKRWDAGFTLNGQLVLKKDKVLDIQGNQPCWCVGSIYCEMKYKPNVLDEVINDTYGAPDLTKSYTDKEGGSDEIMLEDESGRVLLVGDFIRSTPFITGVVVGILGMEAEAGTFQVLDICYPTPLPQNPFPAPIATCPTRGKIALVSGLNLNNTSPDRLLRLEILREFLMGRINNKIDDISLIGRLLICGNSVDFDIKSVNKDELMISLTEFSKFLHNILPSISVDIMPGTNDPSDKSLPQQPFHKSLFDKSLESYFNGSNKEILNLVTNPYEFSYNGVDVLAVSGKNINDICKYVIPSNDNGESENKVEEGESNDFKDDIEHRLDLMECTMKWQNIAPTAPDTLWCYPYTDKDPFVLDKWPHVYIVANQPYFGTRVVEIGGKNIKIISVPEFSSTGMIILLDLETLEAETVKIDI"

     misc_feature    154..570
                     /gene="POL31"
                     /locus_tag="YJR006W"
                     /gene_synonym="HUS2; HYS2; SDP5"
                     /note="DNA polymerase delta subunit OB-fold domain;
                     Region: DNA_pol_D_N; pfam18018"
                     /db_xref="CDD:436215"
     misc_feature    637..1455
                     /gene="POL31"
                     /locus_tag="YJR006W"
                     /gene_synonym="HUS2; HYS2; SDP5"
                     /note="PolD2 (DNA polymerase delta, subunit 2), C-terminal
                     domain; Region: MPP_PolD2_C; cd07387"
                     /db_xref="CDD:277333"
     misc_feature    order(655..657,661..663,784..786,904..909,1066..1068,
                     1318..1320)
                     /gene="POL31"
                     /locus_tag="YJR006W"
                     /gene_synonym="HUS2; HYS2; SDP5"
                     /note="putative active site [active]"
                     /db_xref="CDD:277333"
     misc_feature    order(655..657,661..663,784..786,904..906,1066..1068,
                     1318..1320)
                     /gene="POL31"
                     /locus_tag="YJR006W"
                     /gene_synonym="HUS2; HYS2; SDP5"
                     /note="putative metal binding site [ion binding]; other
                     site"
                     /db_xref="CDD:277333"
     misc_feature    order(688..693,700..702,712..714,718..735,841..843,
                     853..855,862..867,874..879,1441..1455)
                     /gene="POL31"
                     /locus_tag="YJR006W"
                     /gene_synonym="HUS2; HYS2; SDP5"
                     /note="PolD3 binding site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:277333"
ORIGIN      

atggacgcattgttaacaaagttcaacgaggatagaagtctccaagatgaaaacttgtcccaaccacgaacgagagtaagaattgttgacgacaatctttacaacaaatcgaatccattccaactatgctataaaaaacgtgactacggttctcagtactaccacatttatcaataccgtctaaagactttcagagaacgcgtcttaaaagaatgtgacaagagatgggatgcgggctttaccttgaatggccaattagttctaaaaaaggacaaagtgttagatattcagggaaaccaaccatgttggtgcgtaggatccatatattgcgagatgaaatataaaccaaacgtactggacgaagtgatcaatgatacatatggtgcaccagatttaactaaaagttatactgataaagagggtggttctgatgaaattatgctcgaagatgaaagtgggagagtgcttctagtgggagattttattcggtctacacccttcatcacgggtgttgttgtaggtatactgggtatggaagctgaagccgggacatttcaagttttagacatatgctaccccactcctttaccacaaaatcctttccctgcaccaattgctacctgccccaccaggggaaaaattgctcttgtttctgggttaaacctcaataatacatcacctgataggctattgagattggagattttgagagaatttttgatggggagaatcaataacaaaattgacgacatttctctaattggtagattactgatatgcggcaactctgttgacttcgacattaaaagcgtgaacaaagacgaattgatgatttctctaactgagttcagtaaatttctacacaacattttgccatctatatctgttgatattatgcccggaaccaatgatccaagtgacaaatctctaccgcaacagcctttccacaaatcattgtttgataagtccctagaatcatacttcaacggttctaacaaagaaattttgaatttagtgaccaacccatatgagtttagctataatggcgtagacgttctagccgtttcaggcaaaaatatcaatgatatttgtaaatacgtaataccatcaaatgataacggagaaagtgaaaacaaggtcgaggagggcgagagtaatgatttcaaagacgatatagagcatcgcctggaccttatggaatgtacaatgaaatggcaaaacatcgcgcccaccgcacctgataccttatggtgttatccgtacactgataaggatccatttgtattggataagtggccccacgtttacattgtggcaaaccagccatattttggaacaagagtagtggaaataggtggtaagaacataaagataatatctgtccctgaattcagttctactgggatgataatattactggatttggaaacgttagaagcagagacggtcaagattgacatttaa

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