LOCUS       NM_001181468            2049 bp    mRNA    linear   PLN 17-DEC-2024
DEFINITION  Saccharomyces cerevisiae S288C Hsp70 family ATPase KAR2 (KAR2),
            partial mRNA.
ACCESSION   NM_001181468
VERSION     NM_001181468.3
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   3  (bases 1 to 2049)
  AUTHORS   Galibert,F., Alexandraki,D., Baur,A., Boles,E., Chalwatzis,N.,
            Chuat,J.C., Coster,F., Cziepluch,C., De Haan,M., Domdey,H.,
            Durand,P., Entian,K.D., Gatius,M., Goffeau,A., Grivell,L.A.,
            Hennemann,A., Herbert,C.J., Heumann,K., Hilger,F., Hollenberg,C.P.,
            Huang,M.E., Jacq,C., Jauniaux,J.C., Katsoulou,C.,
            Karpfinger-Hartl,L. et al.
  TITLE     Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
            X
  JOURNAL   EMBO J. 15 (9), 2031-2049 (1996)
   PUBMED   8641269
REFERENCE   4  (bases 1 to 2049)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 2049)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 2049)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   7  (bases 1 to 2049)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001142).
            
            On Jul 30, 2012 this sequence version replaced NM_001181468.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="X"
     gene            <1..>2049
                     /gene="KAR2"
                     /locus_tag="YJL034W"
                     /gene_synonym="GRP78"
                     /db_xref="GeneID:853418"
     CDS             1..2049
                     /gene="KAR2"
                     /locus_tag="YJL034W"
                     /gene_synonym="GRP78"
                     /EC_number="3.6.4.10"
                     /experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
                     reticulum [PMID:2661018]"
                     /experiment="EXISTENCE:direct assay:GO:0016887 ATP
                     hydrolysis activity [PMID:1325440]"
                     /experiment="EXISTENCE:direct assay:GO:0031204
                     post-translational protein targeting to membrane,
                     translocation [PMID:10367885]"
                     /experiment="EXISTENCE:direct assay:GO:0034099 luminal
                     surveillance complex [PMID:16873065]"
                     /experiment="EXISTENCE:direct assay:GO:0051082 unfolded
                     protein binding [PMID:23704572]"
                     /experiment="EXISTENCE:genetic interaction:GO:0070880
                     fungal-type cell wall beta-glucan biosynthetic process
                     [PMID:9430631]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0000742
                     karyogamy involved in conjugation with cellular fusion
                     [PMID:10069807]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006616
                     SRP-dependent cotranslational protein targeting to
                     membrane, translocation [PMID:7568189]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006986
                     response to unfolded protein [PMID:12808051]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0031204
                     post-translational protein targeting to membrane,
                     translocation [PMID:7568189]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
                     pathway [PMID:11381090]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0051082
                     unfolded protein binding [PMID:12808051]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0070972 protein
                     localization to endoplasmic reticulum [PMID:1996357]"
                     /note="ATPase involved in protein import into the ER; also
                     acts as a chaperone to mediate protein folding in the ER
                     and may play a role in ER export of soluble proteins;
                     regulates the unfolded protein response via interaction
                     with Ire1p"
                     /codon_start=1
                     /product="Hsp70 family ATPase KAR2"
                     /protein_id="NP_012500.3"
                     /db_xref="GeneID:853418"
                     /db_xref="SGD:S000003571"
                     /translation="

MFFNRLSAGKLLVPLSVVLYALFVVILPLQNSFHSSNVLVRGADDVENYGTVIGIDLGTTYSCVAVMKNGKTEILANEQGNRITPSYVAFTDDERLIGDAAKNQVAANPQNTIFDIKRLIGLKYNDRSVQKDIKHLPFNVVNKDGKPAVEVSVKGEKKVFTPEEISGMILGKMKQIAEDYLGTKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVLRIVNEPTAAAIAYGLDKSDKEHQIIVYDLGGGTFDVSLLSIENGVFEVQATSGDTHLGGEDFDYKIVRQLIKAFKKKHGIDVSDNNKALAKLKREAEKAKRALSSQMSTRIEIDSFVDGIDLSETLTRAKFEELNLDLFKKTLKPVEKVLQDSGLEKKDVDDIVLVGGSTRIPKVQQLLESYFDGKKASKGINPDEAVAYGAAVQAGVLSGEEGVEDIVLLDVNALTLGIETTGGVMTPLIKRNTAIPTKKSQIFSTAVDNQPTVMIKVYEGERAMSKDNNLLGKFELTGIPPAPRGVPQIEVTFALDANGILKVSATDKGTGKSESITITNDKGRLTQEEIDRMVEEAEKFASEDASIKAKVESRNKLENYAHSLKNQVNGDLGEKLEEEDKETLLDAANDVLEWLDDNFETAIAEDFDEKFESLSKVAYPITSKLYGGADGSGAADYDDEDEDDDGDYFEHDEL"

     misc_feature    154..1962
                     /gene="KAR2"
                     /locus_tag="YJL034W"
                     /gene_synonym="GRP78"
                     /note="Hsp70 protein; Region: HSP70; pfam00012"
                     /db_xref="CDD:394970"
ORIGIN      

atgtttttcaacagactaagcgctggcaagctgctggtaccactctccgtggtcctgtacgcccttttcgtggtaatattacctttacagaattctttccactcctccaatgttttagttagaggtgccgatgatgtagaaaactacggaactgttatcggtattgacttaggtactacttattcctgtgttgctgtgatgaaaaatggtaagactgaaattcttgctaatgagcaaggtaacagaatcaccccatcttacgtggcattcaccgatgatgaaagattgattggtgatgctgcaaagaaccaagttgctgccaatcctcaaaacaccatcttcgacattaagagattgatcggtttgaaatataacgacagatctgttcagaaggatatcaagcacttgccatttaatgtggttaataaagatgggaagcccgctgtagaagtaagtgtcaaaggagaaaagaaggtttttactccagaagaaatttctggtatgatcttgggtaagatgaaacaaattgccgaagattatttaggcactaaggttacccatgctgtcgttactgttcctgcttatttcaatgacgcgcaaagacaagccaccaaggatgctggtaccatcgctggtttgaacgttttgagaattgttaatgaaccaaccgcagccgccattgcctacggtttggataaatctgataaggaacatcaaattattgtttatgatttgggtggtggtactttcgatgtctctctattgtctattgaaaacggtgttttcgaagtccaagccacttctggtgatactcatttaggtggtgaagattttgactataagatcgttcgtcaattgataaaagctttcaagaagaagcatggtattgatgtgtctgacaacaacaaggccctagctaaattgaagagagaagctgaaaaggctaaacgtgccttgtccagccaaatgtccacccgtattgaaattgactccttcgttgatggtatcgacttaagtgaaaccttgaccagagctaagtttgaggaattaaacctagatctattcaagaagaccttgaagcctgtcgagaaggttttgcaagattctggtttggaaaagaaggatgttgatgatatcgttttggttggtggttctactagaattccaaaggtccaacaattgttagaatcatactttgatggtaagaaggcctccaagggtattaacccagatgaagctgttgcatacggtgcagccgttcaagctggtgtcttatccggtgaagaaggtgtcgaagatattgttttattggatgtcaacgctttgactcttggtattgaaaccactggtggtgtcatgactccattaattaagagaaatactgctattcctacaaagaaatcccaaattttctctactgccgttgacaaccaaccaaccgttatgatcaaggtatacgagggtgaaagagccatgtctaaggacaacaatctattaggtaagtttgaattaaccggcattccaccagcaccaagaggtgtacctcaaattgaagtcacatttgcacttgacgctaatggtattctgaaggtgtctgccacagataagggaactggtaaatccgaatctatcaccatcactaacgataaaggtagattaacccaagaagagattgatagaatggttgaagaggctgaaaaattcgcttctgaagacgcttctatcaaggccaaggttgaatctagaaacaaattagaaaactacgctcactctttgaaaaaccaagttaatggtgacctaggtgaaaaattggaagaagaagacaaggaaaccttattagatgctgctaacgatgttttagaatggttagatgataactttgaaaccgccattgctgaagactttgatgaaaagttcgaatctttgtccaaggtcgcttatccaattacttctaagttgtacggaggtgctgatggttctggtgccgctgattatgacgacgaagatgaagatgacgatggtgattatttcgaacacgacgaattgtag

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