LOCUS       NM_001179307            1299 bp    mRNA    linear   PLN 17-DEC-2024
DEFINITION  Saccharomyces cerevisiae S288C N,N-dimethylaniline monooxygenase
            (FMO1), partial mRNA.
ACCESSION   NM_001179307
VERSION     NM_001179307.3
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   3  (bases 1 to 1299)
  AUTHORS   Johnston,M., Andrews,S., Brinkman,R., Cooper,J., Ding,H., Dover,J.,
            Du,Z., Favello,A., Fulton,L., Gattung,S. et al.
  TITLE     Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
            VIII
  JOURNAL   Science 265 (5181), 2077-2082 (1994)
   PUBMED   8091229
REFERENCE   4  (bases 1 to 1299)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 1299)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 1299)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   7  (bases 1 to 1299)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001140).
            
            On Jul 30, 2012 this sequence version replaced NM_001179307.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="VIII"
     gene            <1..>1299
                     /gene="FMO1"
                     /locus_tag="YHR176W"
                     /db_xref="GeneID:856581"
     CDS             1..1299
                     /gene="FMO1"
                     /locus_tag="YHR176W"
                     /experiment="EXISTENCE:direct assay:GO:0004499
                     N,N-dimethylaniline monooxygenase activity
                     [PMID:10600176]"
                     /experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
                     reticulum membrane [PMID:10077572]"
                     /experiment="EXISTENCE:direct assay:GO:0005829 cytosol
                     [PMID:26928762]"
                     /experiment="EXISTENCE:direct assay:GO:0006457 protein
                     folding [PMID:10077572]"
                     /note="Flavin-containing monooxygenase; localized to the
                     cytoplasmic face of the ER membrane; catalyzes oxidation
                     of biological thiols to maintain the ER redox buffer ratio
                     for correct folding of disulfide-bonded proteins"
                     /codon_start=1
                     /product="N,N-dimethylaniline monooxygenase"
                     /protein_id="NP_012046.4"
                     /db_xref="GeneID:856581"
                     /db_xref="SGD:S000001219"
                     /translation="

MTVNDKKRLAIIGGGPGGLAAARVFSQSLPNFEIEIFVKDYDIGGVWHYPEQKSDGRVMYDHLETNISKKLMQFSGFPFEENVPLYPSRRNIWEYLKAYYKTFIANKDAISIHFSTEVTYLKKKNSQWEITSKDELRTTKSDFDFVIVASGHYSVPKLPTNIAGLDLWFDNKGAFHSKDFKNCEFAREKVVIVVGNGSSGQDIANQLTTVAKKVYNSIKEPASNQLKAKLIETVQTIDSADWKNRSVTLSDGRVLQNIDYIIFATGYYYSFPFIEPSVRLEVLGEGVTGDKHSSVNLHNLWEHMIYVKDPTLSFILTPQLVIPFPLSELQAAIMVEVFCKSLPITTTFDSNACGTHNFPKGKDLEYYAELQELLNSIPRRVGHFEPVVWDDRLIDLRNSSYTDKEERNVLLAEHAQALKKKKAPYFLPAPHT"

     misc_feature    109..>1029
                     /gene="FMO1"
                     /locus_tag="YHR176W"
                     /note="Flavin-binding monooxygenase-like; Region:
                     FMO-like; pfam00743"
                     /db_xref="CDD:395602"
ORIGIN      

atgacagtgaatgacaaaaaaagattggcaatcataggtggggggccaggagggttggcagctgcaagagttttctcgcaaagtcttccaaattttgaaatagaaatatttgtaaaggattacgatattggcggtgtctggcattatccagaacaaaaaagtgatgggagggtcatgtatgaccatttggaaaccaatatatcaaagaagttaatgcagtttagcggctttcccttcgaggaaaacgtccctttatatccgtctcgaagaaacatttgggaatacttgaaagcctattacaaaacgttcattgccaacaaagatgctatcagcatccatttcagcaccgaagttacctacttaaagaagaagaactctcaatgggagataacatctaaagacgaactaaggactactaaatcggattttgacttcgttattgttgcgtctggtcactacagtgtccctaaactaccaactaatatcgcaggtctcgatctatggttcgacaataagggcgcctttcattctaaggatttcaaaaactgcgagtttgcccgtgaaaaagtcgttattgtggtgggaaacggcagttccggccaagatattgccaaccaactaactaccgttgcaaagaaagtatataatagtataaaagagcctgcgagcaatcaactgaaggccaagttaatcgagactgtccaaacaatagacagtgccgattggaaaaatcgttcagttactttatctgatggaagagtacttcagaatatagactacattattttcgccacaggctattattacagctttccatttatagagccctccgttagactggaagtgctaggtgagggtgttactggcgacaagcactcgtctgtcaatctgcacaatttgtgggagcacatgatctatgtaaaagatcctacgctgtcctttatcttgaccccgcagttggtcatcccgttcccgttgtcggaactacaggcggctattatggtcgaagtattctgtaaaagcttaccgataactacaactttcgactctaatgcctgcggaacgcacaacttcccaaagggaaaggacctggaatactatgcagaactacaggaactactgaatagcattccacgtagggtcggtcatttcgaaccagttgtatgggatgatagactgatcgatctaagaaacagtagttatacagacaaagaagaaagaaatgtgcttctagcggaacacgcacaagccctaaagaaaaaaaaagcaccatacttccttccggcgccacatacttaa

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