LOCUS       NM_001179200            1500 bp    mRNA    linear   PLN 17-DEC-2024
DEFINITION  Saccharomyces cerevisiae S288C tRNA (guanine) methyltransferase
            (TRM5), partial mRNA.
ACCESSION   NM_001179200
VERSION     NM_001179200.1
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   3  (bases 1 to 1500)
  AUTHORS   Johnston,M., Andrews,S., Brinkman,R., Cooper,J., Ding,H., Dover,J.,
            Du,Z., Favello,A., Fulton,L., Gattung,S. et al.
  TITLE     Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
            VIII
  JOURNAL   Science 265 (5181), 2077-2082 (1994)
   PUBMED   8091229
REFERENCE   4  (bases 1 to 1500)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 1500)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 1500)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   7  (bases 1 to 1500)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001140).
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="VIII"
     gene            <1..>1500
                     /gene="TRM5"
                     /locus_tag="YHR070W"
                     /db_xref="GeneID:856467"
     CDS             1..1500
                     /gene="TRM5"
                     /locus_tag="YHR070W"
                     /EC_number="2.1.1.228"
                     /experiment="EXISTENCE:direct assay:GO:0002939 tRNA
                     N1-guanine methylation [PMID:17652090]"
                     /experiment="EXISTENCE:direct assay:GO:0005634 nucleus
                     [PMID:14562095]"
                     /experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
                     [PMID:17652090|PMID:11914276|PMID:14562095]"
                     /experiment="EXISTENCE:direct assay:GO:0005759
                     mitochondrial matrix [PMID:17652090]"
                     /experiment="EXISTENCE:direct assay:GO:0052906 tRNA
                     (guanine(37)-N1)-methyltransferase activity
                     [PMID:17652090]"
                     /experiment="EXISTENCE:direct assay:GO:0070901
                     mitochondrial tRNA methylation [PMID:17652090]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0002939 tRNA
                     N1-guanine methylation [PMID:11226173]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0052906 tRNA
                     (guanine(37)-N1)-methyltransferase activity
                     [PMID:11226173]"
                     /note="tRNA(m(1)G37)methyltransferase; methylates a tRNA
                     base adjacent to the anticodon that has a role in
                     prevention of frameshifting; localized to both cytoplasm
                     and mitochondria, and modifies both cytoplasmic and
                     mitochondrial tRNAs; mutations in human ortholog TRMT5 are
                     associated with skeletal muscle respiratory chain
                     deficiencies, and trm5 mutations analogous to disease
                     mutations decrease respiration"
                     /codon_start=1
                     /product="tRNA (guanine) methyltransferase"
                     /protein_id="NP_011937.1"
                     /db_xref="GeneID:856467"
                     /db_xref="SGD:S000001112"
                     /translation="

MKIALPVFQKFNRLISSCKMSGVFPYNPPVNRQMRELDRSFFITKIPMCAVKFPEPKNISVFSKNFKNCILRVPRIPHVVKLNSSKPKDELTSVQNKKLKTADGNNTPVTKGVLLHESIHSVEDAYGKLPEDALAFLKENSAEIVPHEYVLDYDFWKAEEILRAVLPEQFLEEVPTGFTITGHIAHLNLRTEFKPFDSLIGQVILDKNNKIECVVDKVSSIATQFRTFPMKVIAGKSDSLVVEQKESNCTFKFDFSKVYWNSRLHTEHERLVKQYFQPGQVVCDVFAGVGPFAVPAGKKDVIVLANDLNPESYKYLKENIALNKVAKTVKSFNMDGADFIRQSPQLLQQWIQDEEGGKITIPLPLKKRHRSQQHNDQQPPQPRTKELIIPSHISHYVMNLPDSAISFLGNFRGIFAAHTKGATDTIQMPWVHVHCFEKYPPGDQVTEDELHARVHARIIAALKVTADDLPLNAVSLHLVRKVAPTKPMYCASFQLPANV"

     misc_feature    526..1308
                     /gene="TRM5"
                     /locus_tag="YHR070W"
                     /note="Met-10+ like-protein; Region: Met_10; pfam02475"
                     /db_xref="CDD:396850"
ORIGIN      

atgaaaatcgcactgccagtattccaaaaatttaatagattgatcagttcgtgcaagatgtcaggagtgttcccatacaacccacccgtgaatcgccaaatgcgagaattggataggtcatttttcatcaccaagataccaatgtgtgcagtcaagttccccgaacccaaaaatatcagtgtgttttctaaaaacttcaaaaattgcatcttgagggtgccccgtatcccgcacgttgttaagttaaactcatccaagccaaaggacgaactgacaagtgtacagaacaagaaactaaagactgcagatggcaataataccccagtcacgaaaggtgtgctgctacacgaatccatccacagcgtggaggatgcgtatggaaaattgcctgaggacgccttggcgtttcttaaggaaaacagtgcggaaattgtaccacacgaatacgtgctcgattatgatttctggaaagctgaggagattcttcgagccgtgctgcccgagcaattcctggaagaggttccaacgggttttacaatcacaggccacattgcccacttgaacttgcgcaccgagttcaaaccattcgactcgctcatcggacaagtcatccttgacaagaacaacaaaatcgaatgtgtcgtggacaaagtcagctccattgccacacagttcaggacttttcctatgaaagtcattgctgggaaaagcgatagtctagtggtagagcagaaggaatccaactgcactttcaaattcgactttagcaaggtgtactggaactcccgattgcacacagagcatgaaagactcgtcaaacagtacttccagcctggccaagtagtctgtgatgttttcgccggcgtggggccctttgctgtacctgcgggcaagaaggatgtcatcgttttggctaacgacttgaatcctgagagttacaaatacttgaaggagaacatcgcattgaacaaagtggccaagaccgtcaaatccttcaacatggatggcgctgacttcatccgccagtcgccacagttattacaacagtggattcaagatgaagagggcggcaagatcacgatccctctgcctttgaagaaacgacacagatcccagcaacacaatgaccaacaaccgccacaaccacgcaccaaagagctgatcattccgtcacatattagccactatgtcatgaatctaccagacagcgccatttctttcttaggaaacttccgtggcatctttgcagcacacactaaaggcgccactgataccatccaaatgccatgggtgcacgtgcactgttttgagaagtacccgcctggcgaccaagtcactgaggacgagttgcatgcacgcgttcatgcccgtattattgccgcgctcaaggtcactgcagacgatcttccgttgaatgccgtttcgttgcatttggtgcgcaaagttgcacccaccaaaccgatgtattgcgcgagtttccaacttccagctaacgtatga

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