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Previous release (v1)
2024-11-01 08:00:40, GGRNA.v2 : RefSeq release 226 (Sep, 2024)
LOCUS NM_001110810 2732 bp mRNA linear ROD 01-JUN-2024
DEFINITION Rattus norvegicus myosin light chain kinase 3 (Mylk3), mRNA.
ACCESSION NM_001110810 XM_001064628 XM_214645
VERSION NM_001110810.1
KEYWORDS RefSeq; RefSeq Select.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 2732)
AUTHORS Avila-Rodriguez,D., Solano Agama,C., Gonzalez-Pozos,S., Vicente
Mendez-Mendez,J., Ortiz Plata,A., Arreola-Mendoza,L. and
Mendoza-Garrido,M.E.
TITLE The shift in GH3 cell shape and cell motility is dependent on MLCK
and ROCK
JOURNAL Exp Cell Res 354 (1), 1-17 (2017)
PUBMED 28300565
REMARK GeneRIF: We found that a rounded shape with high cortical tension
depends on MLCK and ROCK, whereas in cells with an elongated shape,
MLCK is the primary protein responsible for cell spreading.
Further, in cells with a slow and directionally persistent
motility, MLCK predominates, while rapid and erratic movement is
ROCK-dependent
REFERENCE 2 (bases 1 to 2732)
AUTHORS Eikemo,H., Moltzau,L.R., Hussain,R.I., Nguyen,C.H., Qvigstad,E.,
Levy,F.O., Skomedal,T. and Osnes,J.B.
TITLE CaMKII in addition to MLCK contributes to phosphorylation of
regulatory light chain in cardiomyocytes
JOURNAL Biochem Biophys Res Commun 471 (1), 219-225 (2016)
PUBMED 26809094
REMARK GeneRIF: In conclusion, in addition to MLCK, CaMKII phosphorylates
RLC in cardiomyocytes.
REFERENCE 3 (bases 1 to 2732)
AUTHORS Gao,L., Zheng,Y.J., Gu,S.S., Tan,J.L., Paul,C., Wang,Y.G. and
Yang,H.T.
TITLE Degradation of cardiac myosin light chain kinase by matrix
metalloproteinase-2 contributes to myocardial contractile
dysfunction during ischemia/reperfusion
JOURNAL J Mol Cell Cardiol 77, 102-112 (2014)
PUBMED 25451385
REMARK GeneRIF: Ischemia/reperfusion-activated MMP-2 leads to the
degradation of cardiac MLCK.
REFERENCE 4 (bases 1 to 2732)
AUTHORS Al-Sadi,R., Ye,D., Dokladny,K. and Ma,T.Y.
TITLE Mechanism of IL-1beta-induced increase in intestinal epithelial
tight junction permeability
JOURNAL J Immunol 180 (8), 5653-5661 (2008)
PUBMED 18390750
REFERENCE 5 (bases 1 to 2732)
AUTHORS Chan,J.Y., Takeda,M., Briggs,L.E., Graham,M.L., Lu,J.T.,
Horikoshi,N., Weinberg,E.O., Aoki,H., Sato,N., Chien,K.R. and
Kasahara,H.
TITLE Identification of cardiac-specific myosin light chain kinase
JOURNAL Circ Res 102 (5), 571-580 (2008)
PUBMED 18202317
REFERENCE 6 (bases 1 to 2732)
AUTHORS Seguchi,O., Takashima,S., Yamazaki,S., Asakura,M., Asano,Y.,
Shintani,Y., Wakeno,M., Minamino,T., Kondo,H., Furukawa,H.,
Nakamaru,K., Naito,A., Takahashi,T., Ohtsuka,T., Kawakami,K.,
Isomura,T., Kitamura,S., Tomoike,H., Mochizuki,N. and Kitakaze,M.
TITLE A cardiac myosin light chain kinase regulates sarcomere assembly in
the vertebrate heart
JOURNAL J Clin Invest 117 (10), 2812-2824 (2007)
PUBMED 17885681
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from EU236721.1.
On or before Nov 24, 2007 this sequence version replaced
XM_001064628.1, XM_214645.4.
##Evidence-Data-START##
Transcript exon combination :: EU236721.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMEA5760434, SAMN06621351
[ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
RefSeq Select criteria :: based on conservation, expression,
longest protein
##RefSeq-Attributes-END##
FEATURES Location/Qualifiers
source 1..2732
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="W"
/db_xref="taxon:10116"
/chromosome="19"
/map="19q11"
gene 1..2732
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="myosin light chain kinase 3"
/db_xref="GeneID:291926"
/db_xref="RGD:1305801"
exon 1..723
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
misc_feature 43..45
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="upstream in-frame stop codon"
CDS 238..2598
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/EC_number="2.7.11.18"
/function="phosphorylates MLC-2v"
/note="cardiac-MLCK; cardiac-MyBP-C-associated Ca/CaM
kinase; putative myosin light chain kinase 3"
/codon_start=1
/product="myosin light chain kinase 3"
/protein_id="NP_001104280.1"
/db_xref="GeneID:291926"
/db_xref="RGD:1305801"
/translation="
MSGVSEEDPEGLGPQGLPALGGACLVTVDKKLNVLTEKVDRLLHFQEDVTEKLQCVCQGMDHLEQGLHRLEASQELGLAGPGSTSPAAAQAAWPEVLELVRAVRQEGAQHGARLEALFKMVVAVDRAITLVGSTIQNSKVDDFILQGTVPWRKGSLADGPEENKEQAEVAGVKPKHVLNTGSVQAATSRALWEESQKQDTPVGTVEGLPLIIDTSLKGADLTQAGASLRQGVEALDPGQEPPPAEAESRLPALASEDTGTTLELSVAIDRISEVLTSLRMSQSAGEGTSSSKPDCSEPGPQPLGPLTTDSDIHSDEGLPRISVRMREMTTPEELFETQGGSPIGSAEAPGPGTVLEDQIPKGARPFPPLPKRSCNNGGASAEEATGPGAEPIRGPSLVTRDWRDEPVGTTDLQQGRDPGAVSPEPGKDHAAQGPGRTEAGRRVSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDTLISTSYTVSQHEVLGGGRFGQVHRCTERSTGLALAAKIIKVKNIKDREDVKNEINIMNQLSHVNLIQLYDAFESKNSFTLIMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQTGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETDAETMNFIVNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLNHLIAKASGSNVRLRSQLLLQKYMAQRKWKKHFHVVTAVNRLRKFPTCP"
misc_feature 700..702
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:22673903; propagated from
UniProtKB/Swiss-Prot (E9PT87.1); phosphorylation site"
misc_feature 934..1011
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="propagated from UniProtKB/Swiss-Prot (E9PT87.1);
Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
misc_feature 1072..1182
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="propagated from UniProtKB/Swiss-Prot (E9PT87.1);
Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
misc_feature 1234..1566
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="propagated from UniProtKB/Swiss-Prot (E9PT87.1);
Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
misc_feature 1258..1260
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:22673903; propagated from
UniProtKB/Swiss-Prot (E9PT87.1); phosphorylation site"
misc_feature 1501..1503
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:22673903; propagated from
UniProtKB/Swiss-Prot (E9PT87.1); phosphorylation site"
misc_feature 1666..2448
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="Catalytic domain of the Serine/Threonine Kinase,
Myosin Light Chain Kinase 3; Region: STKc_MLCK3; cd14192"
/db_xref="CDD:271094"
misc_feature order(1699..1713,1717..1719,1723..1725,1762..1764,
1768..1770,1855..1857,1903..1908,1912..1914,1924..1926,
1930..1932,2044..2046,2050..2052,2056..2061,2065..2067,
2101..2106,2113..2115,2158..2169)
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="active site"
/db_xref="CDD:271094"
misc_feature order(1699..1713,1717..1719,1723..1725,1762..1764,
1768..1770,1855..1857,1903..1908,1912..1914,1924..1926,
2056..2061,2065..2067,2101..2106)
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:271094"
misc_feature order(1711..1713,1924..1926,1930..1932,2044..2046,
2050..2052,2056..2058,2113..2115,2158..2169)
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="polypeptide substrate binding site [polypeptide
binding]; other site"
/db_xref="CDD:271094"
misc_feature 2101..2169
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/note="activation loop (A-loop); other site"
/db_xref="CDD:271094"
exon 724..817
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
exon 818..1196
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
exon 1197..1600
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
exon 1601..1706
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
exon 1707..1800
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
exon 1801..1910
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
exon 1911..2052
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
exon 2053..2123
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
exon 2124..2252
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
exon 2253..2405
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
exon 2406..2538
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
exon 2539..2732
/gene="Mylk3"
/gene_synonym="MLCK; RGD1305801"
/inference="alignment:Splign:2.1.0"
ORIGIN
caagggcctgggccaaactcagcctgtcgctctctcagtggttgagtgggagatagacacagagaacttggccctctccgtcccccacacagttcagtcaggaggacaggttggttccctgcagcaaggctcttagcagcggaggacaatggccttccagacgttaccaccaaagtgagaagcagacccttcgtgctccagtttccttgtctttgcccttaccaaaccagaattgggatgtcaggagtttcagaggaggatccagagggtctgggcccccagggtctgccagcgttgggcggagcctgcttagtcaccgtggacaaaaaacttaatgtgctgactgagaaggtcgacagactcttgcatttccaagaagatgtcacagagaagctacagtgtgtgtgccaaggcatggatcacctggaacaaggtctgcatcggctggaggcctcccaggagttgggtctggcagggcccggcagcacttccccagccgctgctcaggccgcatggcctgaggtcctggagctggtgagggccgtgcggcaggagggtgcccagcacggtgccaggctcgaagccctcttcaagatggtggtggctgtggacagggctattactttggtagggtccacaatccagaactccaaagtggatgatttcatcctgcaagggaccgtgccctggaggaaaggcagtctggctgatggccctgaggagaacaaggagcaagcagaagtggctggagtgaagccaaagcatgtgctgaatacaggaagtgtgcaagctgccacttctagggcgctgtgggaagagagccagaagcaggacacacccgtggggacagtggaggggctgcctctcatcatcgatacgtcactgaagggagctgacctcacccaggctggagcctcactgaggcagggagttgaagctcttgacccaggccaagaacccccacccgcagaggcagaatccaggcttcctgcactagccagcgaggacactgggaccaccctggaattgtctgtagcaattgacagaatcagtgaggtcctcactagcctcaggatgtcccaaagtgctggcgaaggaacctcatccagcaagcctgactgttcagagcctggccctcagcccctagggccactaactacagacagtgacattcacagtgatgaaggacttcccaggatctctgtccgtatgcgagagatgactactcctgaggagctgtttgagacccaaggtggcagccccattggctcggcagaagctccaggccctggaactgtgttagaagaccagatccctaaaggagccagaccatttccacccctgccaaagaggagctgcaacaatggtggcgcgagtgcagaggaggcaacagggcctggggctgagcccatcagaggaccaagcttggtcacaagggactggagagatgaacctgttgggaccacagacctgcagcaaggcagagacccaggagcggtgagccctgaacctgggaaggaccatgcagcccagggcccagggagaaccgaagctggaaggagggtgtcttctgctgcagaggctgccatcgtagttctagatgacagcgcagcacccccagccccttttgaacaccgggtagtgagcatcaaagacaccctcatctcgacaagttacacagtgtcccaacacgaagtcttgggagggggccggtttggccaggtgcacaggtgtacagagcggtccacaggccttgcactggcagccaagatcatcaaagtgaagaacataaaggaccgggaggatgtgaagaatgagatcaacatcatgaaccagctcagccacgtaaacttgatccaactttatgatgcttttgagagcaagaacagcttcaccctgatcatggagtatgtggatggaggtgaactcttcgaccggatcacggatgagaagtaccacctgaccgagctggatgtggtcttgttcacaaggcagatctgtgagggtgtgcattacctgcaccagcactacattctgcacctggacctcaagccagagaacatactgtgtgtcagccagactgggcatcaaattaagattattgactttgggctggctagaagatataaacctcgggagaagctaaaggttaactttggtactccagagttcctggctccagaagttgttaactatgagtttgtctcattcccaacagacatgtggagtgtgggagttatcacctacatgctactcagtggtttgtccccatttctaggggaaacagatgcagagaccatgaattttattgtgaactgcagctgggatttcgatgctgataccttcaaagggctgtcagaggaagccaaggactttgtttcacggttgctggtcaaagagaagagttgtaggatgagcgccacacagtgcctgaaacatgagtggttaaatcacctgattgccaaagcctcaggctccaacgttcgcctcagatcccaactactgctgcagaaatatatggctcagcgtaaatggaagaaacatttccatgtggtgactgcagtcaacaggctaagaaaatttccaacgtgtccctaatctacaactgggcctgggagttcctgaggcgacacgcagtggtaatgtgaagagatgactcaggattttatggagtcaggagcttggctgttattgatcttattttgcaaagaatggtggaaggaagaaagaga
//
by
@meso_cacase at
DBCLS
This page is licensed under a
Creative Commons Attribution 4.0 International License (CC BY 4.0).
If you use GGRNA in your work, please cite:
Naito Y, Bono H. (2012)
GGRNA: an ultrafast, transcript-oriented search engine for genes and transcripts.
Nucleic Acids Res., 40, W592-W596.
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