LOCUS       XM_039109632            6254 bp    mRNA    linear   ROD 22-FEB-2024
DEFINITION  PREDICTED: Rattus norvegicus argonaute RISC component 4 (Ago4),
            transcript variant X1, mRNA.
ACCESSION   XM_039109632
VERSION     XM_039109632.2
DBLINK      BioProject: PRJNA1074393
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_086023) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Feb 22, 2024 this sequence version replaced XM_039109632.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_036323735.1-RS_2024_02
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.2
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 02/09/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /bio_material="RGD 61498"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /sex="male"
                     /tissue_type="kidney, spleen, liver"
                     /geo_loc_name="USA: Wisconsin, Milwaukee"
                     /lat_lon="43.05 N 88.04 W"
                     /collected_by="Rebecca Schilling, Melinda Dwinell"
     gene            1..6254
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 1 EST,
                     86 long SRA reads, 2 Proteins"
                     /db_xref="GeneID:298533"
                     /db_xref="RGD:1304583"
     CDS             137..2602
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X1"
                     /protein_id="XP_038965560.1"
                     /db_xref="GeneID:298533"
                     /db_xref="RGD:1304583"
                     /translation="

MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLSEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYNLQLKYSHLPCLQVGQEQKHTYLPLEVKSNSVLGGPDPYLKEFGIVVHNEMTELMGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEIAQELLFSQEVVQDLTSMARELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA"

     misc_feature    212..601
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    632..784
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    785..1135
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(941..943,986..988,1028..1030,1040..1042,1094..1096,
                     1115..1117,1121..1123)
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1166..2473
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1577..1579,1589..1591,1625..1636,1643..1645,
                     1667..1669,1676..1678,1688..1690,1700..1702)
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1781..1783,1787..1789,2027..2029,2441..2443)
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      6254
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

cgccccttgcccagcgctcgcgtcttcgcggcgccgcttcagcgccaatattccggagagcaagcgttacgcggcggcggcggggcccggagcgggaggcgccggggaccggggcgaggcggcccccgccgccgccatggaggcgctgggacccggacctccagccagcctcttccagccaccccggcgtcctggccttggcactgttgggaaaccaatccgactgttagccaatcattttcaggttcagattcctaaaatagatgtgtatcactatgatgtggatattaaaccggaaaaacggcctcgtagagtcaacagggaggtagtagacactatggtgcgacacttcaagatgcagatatttggagatcggcagcctggctatgatggcaaaagaaacatgtacacagcacatccactgccaatcggacgggatagggttgatatggaggtgaccctcccaggtgagggcaaggaccaaacgttcaaagtgtcagtgcaatgggtgtcagttgtgagccttcagctgcttttagaagctttagctggacacctgagtgaagtcccagatgactcagtacaagcccttgacgtgattacaaggcatctcccctctatgaggtacactccagtaggccggtcttttttctcacctcctgaaggttactaccaccctctgggagggggcagagaggtctggttcggctttcaccagtctgtgcgccctgccatgtggaagatgatgctcaacattgatgtatctgcaactgctttctaccgggctcagcccatcattgagttcatgtgtgaggttttagacatccagaacatcaacgagcagacgaaacctctaacagactcccagcgtgtcaagtttaccaaagaaatcaggggtcttaaagttgaggtgactcactgtggacagatgaaacgaaaatatcgagtttgtaatgtgacaagacgaccagccagtcatcaaaccttccctttgcagctagaaaatggtcaagctatggaatgtacagtagctcagtattttaagcaaaaatataatctgcagctgaaatactcccatcttccctgccttcaagtgggacaagagcaaaagcatacatacctgccacttgaggtgaagagcaacagcgtgctgggtggacctgacccatacctgaaggaatttgggatcgttgtgcacaatgagatgacggaactcatgggcagggtgcttccggcgccaatgctgcagtatggaggccggaataaaacagtagccacacccaaccagggtgtctgggacatgcgaggaaagcagttttatgctggcattgaaattaaagtatgggcagtggcttgttttgcgcctcagaaacaatgtagggaagatttgctaaagagcttcactgaccaacttcgtaaaatctccaaggatgcagggatgcccatccagggtcaaccatgtttctgcaaatatgctcaaggtgcagacagtgtggagcccatgtttaaacatctgaaaatgacatatgtgggcctgcagctaatagtggtgattttgcctgggaagacaccagtatatgcggaggtgaagcgtgttggagataccctcctaggtatggccacgcagtgtgtccaggtcaaaaatgttgtgaagacctcgccacaaaccctttccaacctttgtctgaagataaacgcaaagcttggaggaattaacaacgtacttgtgcctcatcaaaggccctcagtgttccagcagcctgtcatcttcctgggagcagatgtcactcatccacctgctggggatgggaagaagccttctattgcagcggtggtgggcagcatggatggccatcccagtcggtactgtgccactgtccgggtgcagacatctcgccaggagattgcccaggagctcctgttcagtcaggaggtcgtgcaggacctgacgagcatggctcgggagctgctgattcagttctataagtccacgcgcttcaagcccacgcgtatcatctactaccgcggaggggtatccgagggacagatgaaacaggtagcttggccagaactaatagcaattcgaaaggcatgtataagtctggaggaagactaccggccaggaataacctacattgtggtacaaaagcgacatcacacacgactcttctgtgcagataaaacagaaagggtggggaaaagtggcaatgtcccagcaggcactacggtggatagcaccatcacacatccatcggagtttgacttttacctctgcagtcatgcaggaattcagggaaccagtcgtccttcacattaccaggtcctgtgggatgacaactgctttaccgcggatgaactgcagctgctgacctaccagctctgccacacctacgtgaggtgcacacgctcagtctccattcctgcccctgcatattatgcccggcttgtagcattccgggcaaggtatcatttggtggacaaagatcatgacagtgcggaaggcagtcacgtgtcaggacagagcaacggccgggatcctcaagccttggctaaggcggtgcagatccaccatgatacccagcacactatgtattttgcctgagagtcttggaaaagaactcaaccaatttggcatcccacgcatcctcaaaatgtttcagatgcctactgcctctcttagtgcacagtgatttcaggtggtccctaccagcagctcagaaaagttgcactgaatctgtactctgtagccctgtctgatgcatcaacaaaatcgagcctttttttttttttaagaaatagatactcatagattatgttttctgatgcactgggcagaatttttacctcaacgtgcgaaggctgattagccttgactttctaaaggactttccaagaagggtttctgtggaatattttgctagctgtggtattcaccgcatctagtcttataagaggagtatttccttttttctgtgttattgactggagtgtgtacataagaacatttggaagagagaaaccaaacgacctggatcatttccatgtaactttttaattgagtgggtccgtagacttcttaatggagatttcttactttgccactgcaaatgcctttgaggagcattaagttttgaaagttttccagaactctgttaccttcactgcgttatctgactgacctctccgggcctgcgctgtagagtacaggctgcctggtgattgcactaatacttggctgagatttgaacaggcagctcccttccttggctggcttttgtgaatctgtgacataagcagatgtgatgtgtgtcacagggacagataacactgccaatggtaaaaagtacccatgttctccatatttgggaaaaaaaagaattagtttttagtatttttccacattttcaaaagtgcccattttatgctgctgtgtggtgtgttagatctaaacgatctttaaacttctctcatagaaaattaactttggcaataagcatttttaaggtctctgctctttttcctcctgacatagttttctagataagatttcagtatgattttatcaactatttcttgtatctcataactgggatattctgaaaccagtttataaagacatggatttccatgtcctttaaataattgtttttttaaaaaacaaaacaaacctattttatagtcagtgtcctgtcactttaaaaaaaaaaaaaagctggaaccaggatgctttctgattggtcaggctttgctgacgtataaggctcattgagcagtactgcacttgaaaagaaagtaccagccagtaaaaaaatagtttccctgggaatcaaggctgcacacacagctgcttacaagacagcctgaagtgtgcttggctttgcccatctctctctcaactaaaactctgtagaagttgatttttttttctctctctctgtaatagattctggattccctcacaggaagacaggacttcttttcttgtcgtaacaaagggagttgatgtaaagcaagtatcagttagaatgaagggaaaatgtatcctgtatttagtgtagatagtacttggtaaaggacaagcgttacatagtcaaggtagtcctacctgcggtttccagcgcacagttactccaacctggaagggtaaggagtgaaaggcaagaggctctatgtagagccacactcctcatggctgaggagggtgctgggaagagacagacgcaatatgctagagtcaattttgcagtctctctgtagaaaccgttggtgggaaacactgggtcagatgataaagggtgtttgcttgaaccctttagttggtccaaggtccaaatctgttaaacgggccctctcaatcccagcaatataaaacactgtttcccttcagtcttttaaacaagtgtgtagatacattaaatgtttacatttccgtgtgctgtcgtcgctcagcggtgccaccagtgtttgctgagactggtctccaagagagtttccaatgtccatctaatcctatagcacgtgagggtgggtgcagacccccttaatctgcagaccgtgggaaaaagcaagtcctgaggctgaagcacaacatagcagtgaagagacagcagacgtctgttctctgagattttccttttctgcaaatacctcacatggattatacaaatcaggacatgttgatgaatggctgactgccacttttattcacacctgttcagggaaagcacagaaaatactctgcagcccagccactctggacccaagaagagggaaaaaacaaaaggaagctttttcagaaaaaggaaaacttgcctggagtctgtgaggcgaagaaccaagagggggctggcagctagccagagggcaccgtacctgcactggagtgcctagggctcgcttgttaacaatacacagaagaaactcttgggatcttccgacataatggaacagtgagcaagccgtgtgcccagacgctccatccaccttcccgcagttggcttcgaggtttgcccatgaacaatgtatctgaatataccgtcagccctcgtatgcaaggccctgggttcggtccccagctccggaaaaaaagaaccaaaaaaaaaaaaaaaaaaaaaaaaaaacaacagaattgcagtcataccatcgcaagccaatctgaatgcatagttttaattgaacaagagttggcaattcaaatagataccccccccccaaaactgactgaattgttatgcatgtgtcaatcaatgggaggtgtgcattctctagaagtagaattaggttgtttggttttttaaaacacagcctatttaggtcctgtcatatagcgaggaagatttcagtgtagcgactctaaggagtgactatttggggctaaggagcacaggaccttacggatgggaaaggcccagatgtcgaaccaattatgttttgttgttgttgatgtgttgtcgttagttgttgaaacccgagctgtttataaagggaagcagccggcttggtgtagccagtgttctgggcaggtgaaccacattcattctcgctgtaaattctacgtgctgcttccaaaggcgatggttcacaagcaggtgtggcctaagtggtctgtaccttaggatctggtgtctggcccatatcctacatgaaaacctgcccacctggctcagctacccactccttcccatggacaaagataaagcactgttttaaccaggctcaccccgtctcccccgccatggttaccctccctccgaggacgctctgagactcccttgcttagtacaaggtggctgctctgctttcattttgagaaagcagaggttagggcgggcgccatattaatactgcttcaacatcaaccattttcttaccccttcctgccgacactcacattctttctgcatccaccagccaaaagtgcttgtgttcatctgaccgatggtgtggtttctgtttctgtatgcttagacttgtaatgctttaaaatgcattttactaatttggactggatgtctgtctccagcgatgcgaggtactttctaagctatgaaggaagggtttgtatccttatgtgagatgagtaacggttgtttaaattttgcagaggttcttgttactgttactggtttggggttttttggtttttggggggggttttgtttgtttgtttgtttgtttgttttggcttgcagggatattttgtgttcatgtgtctgaaaagtggggaggggaataaatggcattcttgtgctaaa

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