LOCUS       XM_039089632            4404 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus piwi-like RNA-mediated gene silencing
            1 (Piwil1), transcript variant X2, mRNA.
ACCESSION   XM_039089632
VERSION     XM_039089632.1
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051347.1) annotated using gene prediction method: Gnomon,
            supported by mRNA and EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /sex="male"
                     /tissue_type="kidney"
                     /country="USA: Wisconsin, Milwaukee, Medical College of
                     Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..4404
                     /gene="Piwil1"
                     /note="piwi-like RNA-mediated gene silencing 1; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 1 mRNA, 1 EST, 3 Proteins, and 100% coverage of the
                     annotated genomic feature by RNAseq alignments, including
                     3 samples with support for all annotated introns"
                     /db_xref="GeneID:363912"
                     /db_xref="RGD:1306980"
     CDS             545..3136
                     /gene="Piwil1"
                     /codon_start=1
                     /product="piwi-like protein 1 isoform X1"
                     /protein_id="XP_038945560.1"
                     /db_xref="GeneID:363912"
                     /db_xref="RGD:1306980"
                     /translation="

MTGRARARARGRARGQETVQHVGAAAQSQQPGYIPPRPQQSPTEGDLVGRGRQRGMVVGATSKSQELQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL"

     misc_feature    590..868
                     /gene="Piwil1"
                     /note="GAGE protein; Region: GAGE; pfam05831"
                     /db_xref="CDD:428642"
     misc_feature    1232..1369
                     /gene="Piwil1"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    1379..1795
                     /gene="Piwil1"
                     /note="This domain is named PAZ after the proteins Piwi
                     Argonaut and Zwille; Region: PAZ; smart00949"
                     /db_xref="CDD:198017"
     misc_feature    order(1511..1513,1550..1552,1574..1576,1586..1588,
                     1640..1642,1688..1690,1694..1696)
                     /gene="Piwil1"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239211"
     misc_feature    1751..3082
                     /gene="Piwil1"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; Region:
                     Piwi_piwi-like_Euk; cd04658"
                     /db_xref="CDD:240016"
     misc_feature    order(2252..2254,2264..2266,2300..2311,2318..2320,
                     2348..2350,2357..2359,2369..2371,2381..2383)
                     /gene="Piwil1"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     misc_feature    order(2444..2446,2450..2452,2654..2656,3056..3058)
                     /gene="Piwil1"
                     /note="active site"
                     /db_xref="CDD:240016"
ORIGIN      

tatgcccacctgtgccttgacctcaacagacctgtacccaagcccaggcttggtcccgacctctctctatgctcgaactttatctatacctctttctctgtctggaacttgtcctctcctcgttgttctgggcctatagcccactctaacctgtttcctttctctccttgtgcccaagcctggcccacatccctcctcctgcctgtcctcacctctctattgttcctggacctcttccacacgtatccctgtgtctgagtctggtccccggttcccccagtccccacctctccatgccaaagcacataatagcatctctctaactatatccccagtctgctacagcctccagctctatccgtatcctcactggttttccacctgcctccctatgctttcacatccgaatcccagtcgttatcacagctgtgttacaatgcagagagacctggctcacttacatggcgcgaccacaccggcatccagcctgcgacacacgtaggattggaaggaggccaagctgaacacctgagctgactggaaaatgactggacgagcccgagcccgggcccgtggcagggcccgaggtcaggagacggtgcagcatgttggggctgctgcacagagccagcaacctgggtacatcccgccgagaccccagcagtcgcccacagagggggacttggttggccgaggacgacagaggggaatggtagtcggagccacgtccaagtcacaagaactgcagatctcagccgggtttcaggagctgtcactggcagagagaggagggcgtcgccgagacttccatgaccttggtgtgaacaccagacagaaccttgaccatgtcaaagagtcaaagacaggctcctctggcatcattgtgaagctgagcaccaaccacttccggctgacctcgcgcccacagtgggccctgtaccagtaccacattgactataaccccctgatggaggcccgcaggctgcgctctgccctgctcttccagcacgaagacctcattggaaggtgtcatgctttcgacgggacaatattgtttttacctaagagactacagcacaaggtcacggaagtattcagtcagactcggaatggggaacacgtgcggattaccatcaccctgaccaacgagctgccgcccacctcacccacctgcctgcagttctataacatcatcttcaggaggctcttgaaaatcatgaatttgcaacaaattggacggaattattacaacccaagtgacccgattgatattccaaatcacaggttggtgatctggcccggcttcaccacctccatacttcaatatgagaacaacatcatgctttgcacagacgtcagccacaaggtcctccgcagcgagaccgttctagacttcatgttcaatctgtaccagcagacggaggagcacaagttccaggagcaagtgtccaaggagctcataggcctcatcgtcctcaccaagtacaacaacaagacctacagggtggatgacatcgactgggaccagaatccaaagagcaccttcaagaaggcagatggctcggaggtcagcttcctggagtactacaggaagcaatacaaccaggagatcacagacttgaagcagccagtgctagtgagccagcccaagcggaggagaggccctggcggcaccctgcctggcccagccatgctcatcccagagctctgctacctcacaggcctgactgataaaatgcgcaatgatttcaacgtgatgaaggacctggcggtgcacacacggctgacccctgagcagcggcagcgggaggtgggccgcctcatcgactacatccacaaggatgacaatgtgcagagagagcttcgagactggggcctaagcttcgattcgaacttgctgtccttctcaggaagaatcttacaatccgagaagatccaccagggtggaaagacgtttgattacaacccacaatttgcagactggtccaaagaaacgagaggcgcgccgctgatcagcgtgaagccgttggataactggctgctgatctatacccgcaggaattatgaagcggccaactcgctgatacagaacctgttcaaagtgactccggccatgggcatccagatgaaaaaggccatcatgatcgaggtggacgacagaacagaagcttatctgagagccttgcagcagaaggtgacatcagacactcagatagttgtctgtctcttgtcaagtaatcggaaggacaagtatgatgccatcaagaagtatttgtgcacagactgccccaccccaagtcagtgtgtggtggcccggacgctgggcaagcagcaaacagtcatggccattgccaccaagatcgccctgcagatgaactgcaagatgggaggcgagctctggcgggtggacatgcctctgaagctggcaatgatcgtgggcattgactgttaccatgacaccacagctgggcggaggtccatcgcaggattcgttgccagcatcaatgaagggatgacccgttggttctcccgctgcgtctttcaggaccgtggacaggagctggtagacggtctgaaggtgtgcttgcaagctgccctgagggcttggagtggctgcaatgaatatatgcccagccgagtcattgtgtaccgagacggtgtgggggacgggcagctgaagaccctggtcaattatgaggtcccacagttcctagactgcctcaagtcagtcgggagaggttacaacccaagactgaccgtgatcgtagtgaagaagcgagtcaatgccaggttttttgctcagtctgggggaagacttcagaaccctcttccggggacagtcatcgatgtggaagtcaccagaccagagtggtatgactttttcatcgtgagccaggcagtgagaagtgggagtgtgtccccaacacactacaatgtcatctacgacagcagtggcctgaagcccgaccacatccagcggctgacgtacaagctgtgtcacgtgtactacaattggcctggagtcatccgagtccctgcaccttgccagtatgcacacaagctggccttcctcgtgggccagagcatccacagagagccgaacctctccctgtccaaccgtctctactacctctaaccagcagcgagggcctacatgctggtttcctccagagctctgccaacctttagttctgtttctcatatgatgttgttctagcgggatgctgggggtgggggttgggggccgcggtttcgttttttttttttttttttagttggtttgtagtggttatagagttaactaaggttcactgtttgctttgtttctcatagagcgttgtgacaatttgcttggcagagtggtggcagggggccgtggtaccacagatagactttcctaaagcagccgagtcggcaggttctcggaggtgctcctgggagcacgtcctactgtacgctgcgctctctctctctctctctctctctctctctctctctctctctctctctctctgcatgtttgaatttaaaggaggaaaagacgcaaggcaaaaatacccagagcatcctaggctgatgctatgtgggactctgaccctctgacacctcagatcctaaagataaaaaggccgggggtgcctctctgaatttcttttaagagttaggttatagggaatgaaaagaattgttgagggttggggatttagctcagtggtagagcgcttgcctagcaagcgcaaggccctgggttcggtccccagctccgaaaaaaaagaaaaaagaaaaaaaaataataattgttgaaagttttaaacttttctccatgaaagtgttttaagactgatgaggccggtggtggaacgagtgttgttttctttcgcaattttctcaccgagtgaaattagcgaccctcactttatttaaacagtttttagttttcgaaggcccgtgttttctttgtttttaaactgcagaagaccactctgggataggattccactcccgaaagtaaggtgtcgggcgtggtggcacacacctgtgatctcagcacttgggaggtgaagcccggggaaccccaagttcccggtcagcctaggctacatagtgagaccttgtcttaacaaacaggaaaataaaagaaaaatggagcagttatgagttatgggtgtcagtttctgcaggagactctgggagtaagagctgcccctgtccccagaggcagctggtgggccaattcaggtgccaggcaaggtatggccacatatgtttgtctggctctttggcagtgaaggaggcttggagttatgtcacgagagcagcttgaagtggctacagtgtgatgagttttacacaatggcagtgtgtgcccccggcacagctcaataaagtttctgtctcacttta

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