LOCUS       NM_022937               1447 bp    mRNA    linear   ROD 14-JUL-2023
DEFINITION  Rattus norvegicus double C2 domain alpha (Doc2a), mRNA.
ACCESSION   NM_022937
VERSION     NM_022937.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Yao J, Gaffaney JD, Kwon SE and Chapman ER.
  TITLE     Doc2 is a Ca2+ sensor required for asynchronous neurotransmitter
            release
  JOURNAL   Cell 147 (3), 666-677 (2011)
   PUBMED   22036572
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Pang ZP, Bacaj T, Yang X, Zhou P, Xu W and Sudhof TC.
  TITLE     Doc2 supports spontaneous synaptic transmission by a
            Ca(2+)-independent mechanism
  JOURNAL   Neuron 70 (2), 244-251 (2011)
   PUBMED   21521611
REFERENCE   3  (bases 1 to 1447)
  AUTHORS   Higashio H, Nishimura N, Ishizaki H, Miyoshi J, Orita S, Sakane A
            and Sasaki T.
  TITLE     Doc2 alpha and Munc13-4 regulate Ca(2+) -dependent secretory
            lysosome exocytosis in mast cells
  JOURNAL   J Immunol 180 (7), 4774-4784 (2008)
   PUBMED   18354201
REFERENCE   4  (bases 1 to 1447)
  AUTHORS   Korteweg N, Denekamp FA, Verhage M and Burbach JP.
  TITLE     Different spatiotemporal expression of DOC2 genes in the developing
            rat brain argues for an additional, nonsynaptic role of DOC2B in
            early development
  JOURNAL   Eur J Neurosci 12 (1), 165-171 (2000)
   PUBMED   10651871
REFERENCE   5  (bases 1 to 1447)
  AUTHORS   Nagano F, Orita S, Sasaki T, Naito A, Sakaguchi G, Maeda M,
            Watanabe T, Kominami E, Uchiyama Y and Takai Y.
  TITLE     Interaction of Doc2 with tctex-1, a light chain of cytoplasmic
            dynein. Implication in dynein-dependent vesicle transport
  JOURNAL   J Biol Chem 273 (46), 30065-30068 (1998)
   PUBMED   9804756
REFERENCE   6  (bases 1 to 1447)
  AUTHORS   Verhage M, de Vries KJ, Roshol H, Burbach JP, Gispen WH and Sudhof
            TC.
  TITLE     DOC2 proteins in rat brain: complementary distribution and proposed
            function as vesicular adapter proteins in early stages of secretion
  JOURNAL   Neuron 18 (3), 453-461 (1997)
   PUBMED   9115738
REFERENCE   7  (bases 1 to 1447)
  AUTHORS   Naito A, Orita S, Wanaka A, Sasaki T, Sakaguchi G, Maeda M,
            Igarashi H, Tohyama M and Takai Y.
  TITLE     Molecular cloning of mouse Doc2alpha and distribution of its mRNA
            in adult mouse brain
  JOURNAL   Brain Res Mol Brain Res 44 (2), 198-204 (1997)
   PUBMED   9073161
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JACYVU010000044.1.
            
            On Nov 27, 2020 this sequence version replaced NM_022937.2.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132291,
                              SAMD00132293 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-330               JACYVU010000044.1  8387647-8387976
            331-410             JACYVU010000044.1  8388332-8388411
            411-485             JACYVU010000044.1  8388589-8388663
            486-595             JACYVU010000044.1  8388774-8388883
            596-722             JACYVU010000044.1  8390017-8390143
            723-782             JACYVU010000044.1  8390223-8390282
            783-946             JACYVU010000044.1  8390365-8390528
            947-1028            JACYVU010000044.1  8390616-8390697
            1029-1125           JACYVU010000044.1  8390787-8390883
            1126-1447           JACYVU010000044.1  8390966-8391287
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q36"
     gene            1..1447
                     /gene="Doc2a"
                     /note="double C2 domain alpha"
                     /db_xref="GeneID:65031"
                     /db_xref="RGD:620518"
     exon            1..330
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..1271
                     /gene="Doc2a"
                     /note="doc2-alpha; double C2, alpha; double C2-like
                     domains, alpha"
                     /codon_start=1
                     /product="double C2-like domain-containing protein alpha"
                     /protein_id="NP_075226.1"
                     /db_xref="GeneID:65031"
                     /db_xref="RGD:620518"
                     /translation="

MRGRRGDRMTINIQEHMAINVCPGPIRPIRQISDYFPRRGPGPEGGGGGRGFGEAPAHLAPLALAPPAALLGATTPDDGAEVDSYDSDDTTALGTLEFDLLYDQASCMLHCRILRAKGLKPMDFNGLADPYVKLHLLPGACKANKLKTKTQRNTLNPVWNEELTYSGITDDDITHKVLRISVCDEDKLSHNEFIGEIRVPLRRLKPSQKKHFNICLERQVPLPSPSSMSAALRGISCYLKELEQAEQGPGLLEERGRILLSLSYSSRRHGLLVGIVRCAHLAAMDVNGYSDPYVKTYLRPDVDKKSKHKTCVKKKTLNPEFNEEFFYEMELSTLATKTLEVTVWDYDIGKSNDFIGGVSLGPGARGEAQKHWRDCLHQPDTAVERWHTLTSELPPAAGALPLA"

     misc_feature    60..335
                     /gene="Doc2a"
                     /note="propagated from UniProtKB/Swiss-Prot (P70611.1);
                     Region: Interaction with UNC13D and DYNLT1.
                     /evidence=ECO:0000250"
     misc_feature    336..707
                     /gene="Doc2a"
                     /note="C2 domain first repeat present in Rabphilin and
                     Double C2 domain; Region: C2A_Rabphilin_Doc2; cd04035"
                     /db_xref="CDD:176000"
     misc_feature    order(426..428,444..446,609..611,615..617,633..635)
                     /gene="Doc2a"
                     /note="Ca2+ binding site [ion binding]; other site"
                     /db_xref="CDD:176000"
     misc_feature    711..1268
                     /gene="Doc2a"
                     /note="propagated from UniProtKB/Swiss-Prot (P70611.1);
                     Region: Interaction with UNC13D. /evidence=ECO:0000250"
     misc_feature    828..1226
                     /gene="Doc2a"
                     /note="C2 domain second repeat present in Rabphilin and
                     Double C2 domain; Region: C2B_Rabphilin_Doc2; cd08384"
                     /db_xref="CDD:176030"
     misc_feature    order(912..914,930..932,1092..1094,1098..1100,1116..1118)
                     /gene="Doc2a"
                     /note="Ca2+ binding site [ion binding]; other site"
                     /db_xref="CDD:176030"
     exon            331..410
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            411..485
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            486..595
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            596..722
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            723..782
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            783..946
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1028
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1125
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1447
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

cctacagaccccagccggccactccagctctacacccgtctcccagccagaggtgctgcatgaggggccgcaggggcgatcgcatgaccatcaacatccaggagcacatggccatcaacgtgtgccctggacccatcaggcccatccgccagatctccgactacttccctcgcagggggccaggaccagagggtggcggcggcggcaggggcttcggggaagccccagctcatctggcccctctggctctggccccccctgcggctctccttggggccactacacccgacgatggagctgaggtagacagctacgactcggatgataccactgccctgggcacgctggaatttgaccttctctatgatcaggcttcctgcatgctgcactgtagaatcctcagggccaagggcctcaagcccatggatttcaacggcctggctgacccctatgtaaagctgcacctcctgccaggagcctgcaaggccaataagctaaaaaccaagacacagaggaacacactgaacccagtgtggaacgaggagctgacgtacagtggaatcacagatgacgacatcacccacaaggtgctcaggatctcggtctgtgatgaggacaagctgagtcacaatgaatttattggggagatccgagtgcccctccgccgcctcaagccttcacagaagaagcattttaacatctgccttgagcgtcaggtcccgcttccttcgccctcttcaatgtctgcggcgctgaggggcatatcctgttacctgaaggagctagagcaggcagagcagggacctgggctgttggaagagcgcgggcgcatcctgctgagcctcagctacagctctcggcggcatgggctgctggtgggcattgttcgctgtgctcacctggctgcaatggatgttaacggctactctgacccttatgtgaagacgtacttgagaccagacgtggataagaaatccaagcataagacgtgtgtaaagaagaagacgctgaacccggaatttaatgaggaattcttttatgagatggaactctccactctggctaccaagaccctggaagtcacagtctgggactacgacattggcaaatccaatgacttcataggtggtgtgtctctggggccaggagcccggggagaggcccagaaacactggagagactgtctacatcagccggacacagcagtggagcgctggcataccctgaccagcgagctgcccccagcagcaggggcgttgccgttggcctgaacagactgcagctgcccagcacaggccctctgcggccgggtccagtacccaaccttcgcacgagtgtgttgcacgtttacatgggtgggacgcccctgccccacactacctgtcttatttttgtgagtctctgtgaccgtgggtctatcttcttgtgagggatgtggagagctata

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