LOCUS       NM_001394371            6622 bp    mRNA    linear   ROD 23-MAR-2023
DEFINITION  Rattus norvegicus argonaute RISC component 4 (Ago4), mRNA.
ACCESSION   NM_001394371 XM_006238862
VERSION     NM_001394371.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Gu S, Jin L, Huang Y, Zhang F and Kay MA.
  TITLE     Slicing-independent RISC activation requires the argonaute PAZ
            domain
  JOURNAL   Curr Biol 22 (16), 1536-1542 (2012)
   PUBMED   22795694
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Modzelewski AJ, Holmes RJ, Hilz S, Grimson A and Cohen PE.
  TITLE     AGO4 regulates entry into meiosis and influences silencing of sex
            chromosomes in the male mouse germline
  JOURNAL   Dev Cell 23 (2), 251-264 (2012)
   PUBMED   22863743
REFERENCE   3  (bases 1 to 6622)
  AUTHORS   Ender C and Meister G.
  TITLE     Argonaute proteins at a glance
  JOURNAL   J Cell Sci 123 (Pt 11), 1819-1823 (2010)
   PUBMED   20484662
  REMARK    Review article
REFERENCE   4  (bases 1 to 6622)
  AUTHORS   Yoda M, Kawamata T, Paroo Z, Ye X, Iwasaki S, Liu Q and Tomari Y.
  TITLE     ATP-dependent human RISC assembly pathways
  JOURNAL   Nat Struct Mol Biol 17 (1), 17-23 (2010)
   PUBMED   19966796
REFERENCE   5  (bases 1 to 6622)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (bases 1 to 6622)
  AUTHORS   Rybak A, Fuchs H, Hadian K, Smirnova L, Wulczyn EA, Michel G,
            Nitsch R, Krappmann D and Wulczyn FG.
  TITLE     The let-7 target gene mouse lin-41 is a stem cell specific E3
            ubiquitin ligase for the miRNA pathway protein Ago2
  JOURNAL   Nat Cell Biol 11 (12), 1411-1420 (2009)
   PUBMED   19898466
REFERENCE   7  (bases 1 to 6622)
  AUTHORS   Chi SW, Zang JB, Mele A and Darnell RB.
  TITLE     Argonaute HITS-CLIP decodes microRNA-mRNA interaction maps
  JOURNAL   Nature 460 (7254), 479-486 (2009)
   PUBMED   19536157
REFERENCE   8  (bases 1 to 6622)
  AUTHORS   Wu L, Fan J and Belasco JG.
  TITLE     Importance of translation and nonnucleolytic ago proteins for
            on-target RNA interference
  JOURNAL   Curr Biol 18 (17), 1327-1332 (2008)
   PUBMED   18771919
REFERENCE   9  (bases 1 to 6622)
  AUTHORS   Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G and
            Tuschl T.
  TITLE     Human Argonaute2 mediates RNA cleavage targeted by miRNAs and
            siRNAs
  JOURNAL   Mol Cell 15 (2), 185-197 (2004)
   PUBMED   15260970
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000162.1.
            
            On Aug 31, 2021 this sequence version replaced XM_006238862.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed/partial sample support SAMD00132261,
                              SAMD00132262 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-402               JACYVU010000162.1  53374796-53375197   c
            403-568             JACYVU010000162.1  53365359-53365524   c
            569-689             JACYVU010000162.1  53362141-53362261   c
            690-871             JACYVU010000162.1  53358642-53358823   c
            872-1008            JACYVU010000162.1  53358341-53358477   c
            1009-1143           JACYVU010000162.1  53358109-53358243   c
            1144-1231           JACYVU010000162.1  53357135-53357222   c
            1232-1379           JACYVU010000162.1  53352859-53353006   c
            1380-1499           JACYVU010000162.1  53352245-53352364   c
            1500-1628           JACYVU010000162.1  53351976-53352104   c
            1629-1762           JACYVU010000162.1  53351653-53351786   c
            1763-1947           JACYVU010000162.1  53350270-53350454   c
            1948-2107           JACYVU010000162.1  53348631-53348790   c
            2108-2423           JACYVU010000162.1  53344761-53345076   c
            2424-2558           JACYVU010000162.1  53344491-53344625   c
            2559-2660           JACYVU010000162.1  53335965-53336066   c
            2661-2860           JACYVU010000162.1  53335507-53335706   c
            2861-6622           JACYVU010000162.1  53329745-53333506   c
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..6622
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="argonaute RISC component 4"
                     /db_xref="GeneID:298533"
                     /db_xref="RGD:1304583"
     exon            1..402
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    207..209
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="upstream in-frame stop codon"
     CDS             384..2969
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="eukaryotic translation initiation factor 2C, 4;
                     argonaute 4, RISC catalytic component; argonaute RISC
                     catalytic component 4; protein argonaute-4-like"
                     /codon_start=1
                     /product="protein argonaute-4"
                     /protein_id="NP_001381300.1"
                     /db_xref="GeneID:298533"
                     /db_xref="RGD:1304583"
                     /translation="

MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLSEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYNLQLKYSHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSVLGGPDPYLKEFGIVVHNEMTELMGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEIAQELLFSQEVVQDLTSMARELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA"

     misc_feature    459..848
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    879..1031
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    1032..1394
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1188..1190,1233..1235,1275..1277,1287..1289,
                     1341..1343,1362..1364,1368..1370)
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1533..2840
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1944..1946,1956..1958,1992..2003,2010..2012,
                     2034..2036,2043..2045,2055..2057,2067..2069)
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2148..2150,2154..2156,2394..2396,2808..2810)
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="active site"
                     /db_xref="CDD:240015"
     exon            403..568
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            569..689
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            690..871
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1008
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1143
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1231
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1379
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1499
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1628
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1762
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1763..1947
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2107
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2108..2423
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2424..2558
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2559..2660
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2661..2860
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2861..6622
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

accccgccccctgcctcggacctgctcgctggaccctgcgcgtgccgggctgcgcacgcccccttcgccagccgggaccccggaccgcacgcgcctggcgccccctccctgcccggcgggcgcgcgccgcctcccgctcccgctccagctggcggcggcggcggcggcccggattgtttttgttgttgccgaggccggaagagccgtgagagagccgggtccctgtccccggaccgggcactgccgccgccccttgcccagcgctcgcgtcttcgcggcgccgcttcagcgccaatattccggagagcaagcgttacgcggcggcggcggggcccggagcgggaggcgccggggaccggggcgaggcggcccccgccgccgccatggaggcgctgggacccggacctccagccagcctcttccagccaccccggcgtcctggccttggcactgttgggaaaccaatccgactgttagccaatcattttcaggttcagattcctaaaatagatgtgtatcactatgatgtggatattaaaccggaaaaacggcctcgtagagtcaacagggaggtagtagacactatggtgcgacacttcaagatgcagatatttggagatcggcagcctggctatgatggcaaaagaaacatgtacacagcacatccactgccaatcggacgggatagggttgatatggaggtgaccctcccaggtgagggcaaggaccaaacgttcaaagtgtcagtgcaatgggtgtcagttgtgagccttcagctgcttttagaagctttagctggacacctgagtgaagtcccagatgactcagtacaagcccttgacgtgattacaaggcatctcccctctatgaggtacactccagtaggccggtcttttttctcacctcctgaaggttactaccaccctctgggagggggcagagaggtctggttcggctttcaccagtctgtgcgccctgccatgtggaagatgatgctcaacattgatgtatctgcaactgctttctaccgggctcagcccatcattgagttcatgtgtgaggttttagacatccagaacatcaacgagcagacgaaacctctaacagactcccagcgtgtcaagtttaccaaagaaatcaggggtcttaaagttgaggtgactcactgtggacagatgaaacgaaaatatcgagtttgtaatgtgacaagacgaccagccagtcatcaaaccttccctttgcagctagaaaatggtcaagctatggaatgtacagtagctcagtattttaagcaaaaatataatctgcagctgaaatactcccatcttccctgccttcaagtgggacaagagcaaaagcatacatacctgccacttgaggtctgtaatatagtagcagggcagagatgtataaagaagctcacagacaaccagacatccaccatgatcaaagccacagcgagatctgctcctgacagacaggaggaaatcagtagactggtgaagagcaacagcgtgctgggtggacctgacccatacctgaaggaatttgggatcgttgtgcacaatgagatgacggaactcatgggcagggtgcttccggcgccaatgctgcagtatggaggccggaataaaacagtagccacacccaaccagggtgtctgggacatgcgaggaaagcagttttatgctggcattgaaattaaagtatgggcagtggcttgttttgcgcctcagaaacaatgtagggaagatttgctaaagagcttcactgaccaacttcgtaaaatctccaaggatgcagggatgcccatccagggtcaaccatgtttctgcaaatatgctcaaggtgcagacagtgtggagcccatgtttaaacatctgaaaatgacatatgtgggcctgcagctaatagtggtgattttgcctgggaagacaccagtatatgcggaggtgaagcgtgttggagataccctcctaggtatggccacgcagtgtgtccaggtcaaaaatgttgtgaagacctcgccacaaaccctttccaacctttgtctgaagataaacgcaaagcttggaggaattaacaacgtacttgtgcctcatcaaaggccctcagtgttccagcagcctgtcatcttcctgggagcagatgtcactcatccacctgctggggatgggaagaagccttctattgcagcggtggtgggcagcatggatggccatcccagtcggtactgtgccactgtccgggtgcagacatctcgccaggagattgcccaggagctcctgttcagtcaggaggtcgtgcaggacctgacgagcatggctcgggagctgctgattcagttctataagtccacgcgcttcaagcccacgcgtatcatctactaccgcggaggggtatccgagggacagatgaaacaggtagcttggccagaactaatagcaattcgaaaggcatgtataagtctggaggaagactaccggccaggaataacctacattgtggtacaaaagcgacatcacacacgactcttctgtgcagataaaacagaaagggtggggaaaagtggcaatgtcccagcaggcactacggtggatagcaccatcacacatccatcggagtttgacttttacctctgcagtcatgcaggaattcagggaaccagtcgtccttcacattaccaggtcctgtgggatgacaactgctttaccgcggatgaactgcagctgctgacctaccagctctgccacacctacgtgaggtgcacacgctcagtctccattcctgcccctgcatattatgcccggcttgtagcattccgggcaaggtatcatttggtggacaaagatcatgacagtgcggaaggcagtcacgtgtcaggacagagcaacggccgggatcctcaagccttggctaaggcggtgcagatccaccatgatacccagcacactatgtattttgcctgagagtcttggaaaagaactcaaccaatttggcatcccacgcatcctcaaaatgtttcagatgcctactgcctctcttagtgcacagtgatttcaggtggtccctaccagcagctcagaaaagttgcactgaatctgtactctgtagccctgtctgatgcatcaacaaaatcgagcctttttttttttttaagaaatagatactcatagattatgttttctgatgcactgggcagaatttttacctcaacgtgcgaaggctgattagccttgactttctaaaggactttccaagaagggtttctgtggaatattttgctagctgtggtattcaccgcatctagtcttataagaggagtatttccttttttctgtgttattgactggagtgtgtacataagaacatttggaagagagaaaccaaacgacctggatcatttccatgtaactttttaattgagtgggtccgtagacttcttaatggagatttcttactttgccactgcaaatgcctttgaggagcattaagttttgaaagttttccagaactctgttaccttcactgcgttatctgactgacctctccgggcctgcgctgtagagtacaggctgcctggtgattgcactaatacttggctgagatttgaacaggcagctcccttccttggctggcttttgtgaatctgtgacataagcagatgtgatgtgtgtcacagggacagataacactgccaatggtaaaaagtacccatgttctccatatttgggaaaaaaaagaattagtttttagtatttttccacattttcaaaagtgcccattttatgctgctgtgtggtgtgttagatctaaacgatctttaaacttctctcatagaaaattaactttggcaataagcatttttaaggtctctgctctttttcctcctgacatagttttctagataagatttcagtatgattttatcaactatttcttgtatctcataactgggatattctgaaaccagtttataaagacatggatttccatgtcctttaaataattgtttttttaaaaaacaaaacaaacctattttatagtcagtgtcctgtcactttaaaaaaaaaaaaaagctggaaccaggatgctttctgattggtcaggctttgctgacgtataaggctcattgagcagtactgcacttgaaaagaaagtaccagccagtaaaaaaatagtttccctgggaatcaaggctgcacacacagctgcttacaagacagcctgaagtgtgcttggctttgcccatctctctctcaactaaaactctgtagaagttgatttttttttctctctctctgtaatagattctggattccctcacaggaagacaggacttcttttcttgtcgtaacaaagggagttgatgtaaagcaagtatcagttagaatgaagggaaaatgtatcctgtatttagtgtagatagtacttggtaaaggacaagcgttacatagtcaaggtagtcctacctgcggtttccagcgcacagttactccaacctggaagggtaaggagtgaaaggcaagaggctctatgtagagccacactcctcatggctgaggagggtgctgggaagagacagacgcaatatgctagagtcaattttgcagtctctctgtagaaaccgttggtgggaaacactgggtcagatgataaagggtgtttgcttgaaccctttagttggtccaaggtccaaatctgttaaacgggccctctcaatcccagcaatataaaacactgtttcccttcagtcttttaaacaagtgtgtagatacattaaatgtttacatttccgtgtgctgtcgtcgctcagcggtgccaccagtgtttgctgagactggtctccaagagagtttccaatgtccatctaatcctatagcacgtgagggtgggtgcagacccccttaatctgcagaccgtgggaaaaagcaagtcctgaggctgaagcacaacatagcagtgaagagacagcagacgtctgttctctgagattttccttttctgcaaatacctcacatggattatacaaatcaggacatgttgatgaatggctgactgccacttttattcacacctgttcagggaaagcacagaaaatactctgcagcccagccactctggacccaagaagagggaaaaaacaaaaggaagctttttcagaaaaaggaaaacttgcctggagtctgtgaggcgaagaaccaagagggggctggcagctagccagagggcaccgtacctgcactggagtgcctagggctcgcttgttaacaatacacagaagaaactcttgggatcttccgacataatggaacagtgagcaagccgtgtgcccagacgctccatccaccttcccgcagttggcttcgaggtttgcccatgaacaatgtatctgaatataccgtcagccctcgtatgcaaggccctgggttcggtccccagctccggaaaaaaagaaccaaaaaaaaaaaaaaaaaaaaaaaaaaaacaacagaattgcagtcataccatcgcaagccaatctgaatgcatagttttaattgaacaagagttggcaattcaaatagataccccccccccaaaactgactgaattgttatgcatgtgtcaatcaatgggaggtgtgcattctctagaagtagaattaggttgtttggttttttaaaacacagcctatttaggtcctgtcatatagcgaggaagatttcagtgtagcgactctaaggagtgactatttggggctaaggagcacaggaccttacggatgggaaaggcccagatgtcgaaccaattatgttttgttgttgttgatgtgttgtcgttagttgttgaaacccgagctgtttataaagggaagcagccggcttggtgtagccagtgttctgggcaggtgaaccacattcattctcgctgtaaattctacgtgctgcttccaaaggcgatggttcacaagcaggtgtggcctaagtggtctgtaccttaggatctggtgtctggcccatatcctacatgaaaacctgcccacctggctcagctacccactccttcccatggacaaagataaagcactgttttaaccaggctcaccccgtctcccccgccatggttaccctccctccgaggacgctctgagactcccttgcttagtacaaggtggctgctctgctttcattttgagaaagcagaggttagggcgggcgccatattaatactgcttcaacatcaaccattttcttaccccttcctgccgacactcacattctttctgcatccaccagccaaaagtgcttgtgttcatctgaccgatggtgtggtttctgtttctgtatgcttagacttgtaatgctttaaaatgcattttactaatttggactggatgtctgtctccagcgatgcgaggtactttctaagctatgaaggaagggtttgtatccttatgtgagatgagtaacggttgtttaaattttgcagaggttcttgttactgttactggtttggggttttttggtttttggggggggttttgtttgtttgtttgtttgtttgttttggcttgcagggatattttgtgttcatgtgtctgaaaagtggggaggggaataaatggcattcttgtgctaaa

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