LOCUS       NM_001308265            5190 bp    mRNA    linear   ROD 01-OCT-2024
DEFINITION  Rattus norvegicus zinc finger E-box binding homeobox 1 (Zeb1),
            mRNA.
ACCESSION   NM_001308265
VERSION     NM_001308265.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Sun,S., Yan,T., Yang,N., Wu,J. and Liu,Z.
  TITLE     Regulation of osteoclast differentiation and inflammatory signaling
            by TCF8 in periodontitis
  JOURNAL   Oral Dis 30 (4), 2580-2591 (2024)
   PUBMED   37246926
  REMARK    GeneRIF: Regulation of osteoclast differentiation and inflammatory
            signaling by TCF8 in periodontitis.
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Souza,C.S., Deluque,A.L., Oliveira,B.M., Maciel,A.L.D.,
            Giovanini,C., Boer,P.A., de Paula,F.J.A., Costa,R.S.,
            Franscecato,H.D.C., de Almeida,L.F. and Coimbra,T.M.
  TITLE     Vitamin D deficiency contributes to the diabetic kidney disease
            progression via increase ZEB1/ZEB2 expressions
  JOURNAL   Nutr Diabetes 13 (1), 9 (2023)
   PUBMED   37391399
  REMARK    GeneRIF: Vitamin D deficiency contributes to the diabetic kidney
            disease progression via increase ZEB1/ZEB2 expressions.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5190)
  AUTHORS   Zhu,X., Yan,F., Liu,L. and Huang,Q.
  TITLE     ZEB1 regulates bone metabolism in osteoporotic rats through
            inducing POLDIP2 transcription
  JOURNAL   J Orthop Surg Res 17 (1), 423 (2022)
   PUBMED   36123704
  REMARK    GeneRIF: ZEB1 regulates bone metabolism in osteoporotic rats
            through inducing POLDIP2 transcription.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5190)
  AUTHORS   Chen,Y.Y., Jiang,K.S., Bai,X.H., Liu,M., Lin,S.Y., Xu,T., Wei,J.Y.,
            Li,D., Xiong,Y.C., Xin,W.J. and Li,Z.Y.
  TITLE     ZEB1 Induces Ddr1 Promoter Hypermethylation and Contributes to the
            Chronic Pain in Spinal Cord in Rats Following Oxaliplatin Treatment
  JOURNAL   Neurochem Res 46 (8), 2181-2191 (2021)
   PUBMED   34032956
  REMARK    GeneRIF: ZEB1 Induces Ddr1 Promoter Hypermethylation and
            Contributes to the Chronic Pain in Spinal Cord in Rats Following
            Oxaliplatin Treatment.
REFERENCE   5  (bases 1 to 5190)
  AUTHORS   Sun,D., Liu,X., Zhu,L. and Zhang,B.
  TITLE     Zinc-finger E-box-binding homeobox 1 alleviates acute kidney injury
            by activating autophagy and the AMPK/mTOR pathway
  JOURNAL   Mol Med Rep 23 (6) (2021)
   PUBMED   33846788
  REMARK    GeneRIF: Zincfinger Eboxbinding homeobox 1 alleviates acute kidney
            injury by activating autophagy and the AMPK/mTOR pathway.
REFERENCE   6  (bases 1 to 5190)
  AUTHORS   Gong,S.G. and Eulenberg,R.L.
  TITLE     Palatal development in Twirler mice
  JOURNAL   Cleft Palate Craniofac J 38 (6), 622-628 (2001)
   PUBMED   11681996
REFERENCE   7  (bases 1 to 5190)
  AUTHORS   Cabanillas,A.M., Smith,G.E. and Darling,D.S.
  TITLE     T3-activation of the rat growth hormone gene is inhibited by a zinc
            finger/homeodomain protein
  JOURNAL   Mol Cell Endocrinol 181 (1-2), 131-137 (2001)
   PUBMED   11476947
REFERENCE   8  (bases 1 to 5190)
  AUTHORS   Takagi,T., Moribe,H., Kondoh,H. and Higashi,Y.
  TITLE     DeltaEF1, a zinc finger and homeodomain transcription factor, is
            required for skeleton patterning in multiple lineages
  JOURNAL   Development 125 (1), 21-31 (1998)
   PUBMED   9389660
REFERENCE   9  (bases 1 to 5190)
  AUTHORS   Higashi,Y., Moribe,H., Takagi,T., Sekido,R., Kawakami,K.,
            Kikutani,H. and Kondoh,H.
  TITLE     Impairment of T cell development in deltaEF1 mutant mice
  JOURNAL   J Exp Med 185 (8), 1467-1479 (1997)
   PUBMED   9126927
REFERENCE   10 (bases 1 to 5190)
  AUTHORS   Cabanillas,A.M. and Darling,D.S.
  TITLE     Alternative splicing gives rise to two isoforms of Zfhep, a zinc
            finger/homeodomain protein that binds T3-response elements
  JOURNAL   DNA Cell Biol 15 (8), 643-651 (1996)
   PUBMED   8769566
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            On Apr 3, 2024 this sequence version replaced NM_001308265.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U51583.1, SRR26643296.36810.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760389,
                                           SAMN12840106 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-81                JAXUCZ010000017.1  56810573-56810653   c
            82-282              JAXUCZ010000017.1  56702472-56702672   c
            283-444             JAXUCZ010000017.1  56661355-56661516   c
            445-647             JAXUCZ010000017.1  56658424-56658626   c
            648-753             JAXUCZ010000017.1  56656453-56656558   c
            754-2558            JAXUCZ010000017.1  56650728-56652532   c
            2559-2736           JAXUCZ010000017.1  56648490-56648667   c
            2737-5190           JAXUCZ010000017.1  56644397-56646850   c
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17q12.1"
     gene            1..5190
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="zinc finger E-box binding homeobox 1"
                     /db_xref="GeneID:25705"
                     /db_xref="RGD:3831"
     exon            1..81
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..3353
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="zfhep; zinc finger homeodomain enhancer-binding
                     protein; TCF-8; transcription factor 8 (represses
                     interleukin 2 expression)"
                     /codon_start=1
                     /product="zinc finger E-box-binding homeobox 1"
                     /protein_id="NP_001295194.1"
                     /db_xref="GeneID:25705"
                     /db_xref="RGD:3831"
                     /translation="

MADGPRCKRRKQANPRRNNVTNYNTVVEANSDSDDEDKLHIVEEESVTDAADCEGGVPDDELPTDQTVLPGGSDRAGSAKNCWQDDVKDDECDSDAENEQNHDPNVEEFLQQQDTAVIYPEAPEEDQRQGTPEASGHDDNGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSLCSYTFAYRTQLERHMTSHKSGREQRHVTQSGGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCISLMPVNGRPRSGLKTSQCSSPSLSTSPGSPTRPQIRQKIENKPLQEPLSVNQIKTEPVDYEFKPIVVASGINCSTPLQNGVFSGGGQLQATSSPQGVVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNIIRQVLENNQASLASKEQEAVSASSIQQGGHSVISAISLPLVDQDGTTKIIINYSLEQPGQLQVVPQNLKKENPAPPKSCKSEKSPEDLTVKSEKDKSFDGAADESTCLLCEDCPGDLNALPELKHYDPEHPAQPPPPAPATEKPESSASSAGNGDLSPSQPPLKNLLSLLKAYYALNAQPSTEELTKIADSVNLPLDVVKKWFEKMQAGQIPGQSLEPPSPGPGSGNIPAKTEEQPQPVDGNEPQEDSTRGQSPLKMTSSPVLPVGSAINGSRSCTSSPSPLNLSSARNPQGYSCVSEGTQEEPQVEPLDLSLPKQQGELLERSTVSSVYQNSVYSVQEEPLNLSCAKKEPQKDSCVTDSEPVVNVVPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSATVSPAMQEPPVKVIQPNGNQDERQDTSSEGVSVEDQNDSDCTPPKKKTRKAENGMYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICRKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKRGAEDRDAMEQEDTGPEALPEVLPTELVGARASPSQADSDERESLTREEDEDSEKEEEEEDKEMEELQEDKECENPQEEEEEEEEEEEEEEEEEEEEAEEAEHEAAAAKTGGAVEEEAAQQAGSFQQKASGSESKRLSEEKTNEA"

     misc_feature    24..341
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62947.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    48..>473
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="GET complex subunit GET2; Region: GET2; pfam08690"
                     /db_xref="CDD:400847"
     misc_feature    114..116
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q62947.2); phosphorylation site"
     misc_feature    120..122
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q62947.2); phosphorylation site"
     misc_feature    390..452
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62947.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    477..542
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="C2H2 Zn finger [structural motif]; Region: C2H2 Zn
                     finger"
                     /db_xref="CDD:275368"
     misc_feature    561..629
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Zinc finger, C2H2 type; Region: zf-C2H2; pfam00096"
                     /db_xref="CDD:395048"
     misc_feature    567..629
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="C2H2 Zn finger [structural motif]; Region: C2H2 Zn
                     finger"
                     /db_xref="CDD:275368"
     misc_feature    681..749
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Zinc finger, C2H2 type; Region: zf-C2H2; pfam00096"
                     /db_xref="CDD:395048"
     misc_feature    687..749
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="C2H2 Zn finger [structural motif]; Region: C2H2 Zn
                     finger"
                     /db_xref="CDD:275368"
     misc_feature    723..794
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Zinc-finger double domain; Region: zf-H2C2_2;
                     pfam13465"
                     /db_xref="CDD:463886"
     misc_feature    855..944
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62947.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    900..902
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q64318; propagated from
                     UniProtKB/Swiss-Prot (Q62947.2); phosphorylation site"
     misc_feature    927..929
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P37275; propagated from
                     UniProtKB/Swiss-Prot (Q62947.2); phosphorylation site"
     misc_feature    1425..1526
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62947.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1596..1721
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62947.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1701..1844
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Region: Homeodomain; pfam00046"
                     /db_xref="CDD:459649"
     misc_feature    1863..2156
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62947.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1992..1994
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P37275; propagated from
                     UniProtKB/Swiss-Prot (Q62947.2); phosphorylation site"
     misc_feature    2013..2015
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q64318; propagated from
                     UniProtKB/Swiss-Prot (Q62947.2); phosphorylation site"
     misc_feature    2034..2036
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q64318; propagated from
                     UniProtKB/Swiss-Prot (Q62947.2); phosphorylation site"
     misc_feature    2055..2057
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q62947.2); phosphorylation site"
     misc_feature    2061..2063
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:P37275; propagated from
                     UniProtKB/Swiss-Prot (Q62947.2); phosphorylation site"
     misc_feature    2067..2069
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q64318; propagated from
                     UniProtKB/Swiss-Prot (Q62947.2); phosphorylation site"
     misc_feature    2523..2642
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62947.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    2670..2732
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="C2H2 Zn finger [structural motif]; Region: C2H2 Zn
                     finger"
                     /db_xref="CDD:275368"
     misc_feature    order(2685..2687,2691..2693,2697..2699,2703..2708,
                     2715..2720,2727..2729,2769..2771,2775..2777,2787..2792,
                     2799..2804,2811..2813,2853..2855,2859..2861,2865..2867,
                     2871..2876,2883..2888)
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]; other site"
                     /db_xref="CDD:275368"
     misc_feature    2706..2783
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Zinc-finger double domain; Region: zf-H2C2_2;
                     pfam13465"
                     /db_xref="CDD:463886"
     misc_feature    2754..2816
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="C2H2 Zn finger [structural motif]; Region: C2H2 Zn
                     finger"
                     /db_xref="CDD:275368"
     misc_feature    2790..2867
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="Zinc-finger double domain; Region: zf-H2C2_2;
                     pfam13465"
                     /db_xref="CDD:463886"
     misc_feature    2838..2891
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="C2H2 Zn finger [structural motif]; Region: C2H2 Zn
                     finger"
                     /db_xref="CDD:275368"
     misc_feature    2925..3350
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62947.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     exon            82..282
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /inference="alignment:Splign:2.1.0"
     exon            283..444
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /inference="alignment:Splign:2.1.0"
     exon            445..647
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /inference="alignment:Splign:2.1.0"
     exon            648..753
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /inference="alignment:Splign:2.1.0"
     exon            754..2558
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /inference="alignment:Splign:2.1.0"
     exon            2559..2736
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /inference="alignment:Splign:2.1.0"
     exon            2737..5190
                     /gene="Zeb1"
                     /gene_synonym="deltaEF1; Tcf8; Zfhx1a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

atttagacacaagcgagaggatcatggcggatggccccaggtgtaagcgcagaaagcaggcgaacccgcggcgcaataacgttacaaattataatactgtggtagaagcaaattcagattcggatgatgaagacaaactccatattgtggaagaagaaagtgtcacagatgcagctgactgtgaaggtggtgtgccagatgatgagctgccaacagaccagacagtgttaccaggaggcagtgacagggcgggcagtgccaagaactgctggcaagacgacgtaaaagatgacgaatgcgactcagatgcagaaaacgagcaaaaccatgatcccaacgtggaagagttcctgcagcaacaggacaccgcggtcatctaccctgaggcacccgaggaggaccagcggcagggcacgccagaagccagcggtcatgatgacaatggaacaccagatgcattttcccagttgctcacctgcccgtattgtgatagaggctacaagcgctttacctctttgaaagaacacattaagtaccgccatgagaagaatgaagacaacttcagctgctccctgtgcagttacacctttgcatacagaacccagcttgaacgtcatatgacatcacataagtcaggaagagagcaaagacatgtgacacagtctggaggtaaccgtaagttcaagtgcaccgagtgtgggaaagcgttcaagtacaaacaccatctgaaggagcacttacggatccacagtggagagaagccatatgaatgcccaaactgcaagaaacgtttttcccattctggctcttatagctcacacataagcagtaagaagtgtattagcttgatgcctgtgaatggtaggcccagatcaggactcaagacatctcagtgttcctcgccatctctttcaacatcaccaggcagtcccacacgcccacagatacggcagaagatagagaataaaccccttcaagaaccgctttctgtaaaccaaatcaaaactgaacctgtggattatgagttcaaacccatagtggttgcttcaggaatcaactgttcaacccctttacaaaatggggtttttagcggtggtggccaattgcaggcaaccagttctcctcagggtgtggtgcaagctgttgttctgccaacagttggtttggtatctcccataagtatcaacttaagtgacattcagaatgtacttaaagtggctgtagatggtaacataatacgacaggttttggagaataatcaagccagtcttgcatccaaagagcaagaagccgtgagtgcttcatccatccagcagggcggccattctgtcatttctgccatcagtcttcctttagttgatcaggatggcacaaccaaaatcatcatcaactacagtcttgagcagcccggccaacttcaggttgttccccagaatctaaagaaagaaaacccagcccctccaaagagctgcaaaagtgagaagtcaccagaagatcttactgtcaagtcagagaaggacaaaagctttgatggggctgcggatgagagcacttgccttttgtgtgaggactgcccaggagacctcaatgcacttccagaactaaagcactatgacccagagcaccctgctcaacctccaccccctgccccagccaccgagaagccagagtcctctgcttcatcagccggcaatggagatttgtctcccagtcagccacctttaaagaaccttctgtcgctcttaaaagcctactatgctctgaatgcacagccaagcacagaagagctcaccaagattgccgattctgtgaacctgccgctggatgtagttaaaaagtggtttgaaaagatgcaagctggacagattcccgggcagtctcttgaaccaccttctccaggaccggggtcaggaaacatccctgcaaaaactgaggagcagcctcaacctgtggatggaaatgagccccaggaagacagcacacgtggacagagtcctctcaagatgacaagctctccagttttaccagtaggatcagccattaacggttccagaagttgcacatcatccccatcccctctaaacctttcctcagccaggaacccgcagggttactcttgtgtgtcagagggtacccaggaggagccccaagtagaacctcttgatctctcactaccaaagcaacagggagagttactggaaaggtcgacagtcagtagcgtttaccagaacagtgtttattctgtccaggaagaacccttgaacttgtcttgtgcaaaaaaggagccacaaaaggacagttgtgttacagactcagaaccagttgtaaatgtagtcccaccaagtgccaaccccataaacattgctattcctacagtcactgcccagttacccacaatcgtggccatcgctgaccagaacagtgttccgtgcttacgggcactcgctgccaacaagcagactattctgattccccaagtggcatatacatactccgctacagtcagcccagccatgcaggagccaccagtgaaggtgatccagccaaatggaaaccaggatgaaagacaagacactagctcagaaggagtctccgtggaggaccagaatgactctgactgcacgccacccaaaaagaaaactcggaaggcagagaatgggatgtacgcatgtgacctgtgtgacaagatatttcagaagagcagctccctgctgagacacaaatatgagcatacaggtaagaggccccacgagtgcggaatctgtagaaaggcatttaaacacaagcatcatttgattgagcacatgcgactgcactctggggaaaagccctaccaatgtgacaagtgtggcaagcgcttctcacactcggggtcttactctcaacacatgaatcaccgctactcctactgcaagagaggagctgaagacagagatgccatggagcaggaagacactgggccggaagccctgccggaagtcctgcccactgagcttgttggtgcccgggcatctccctcacaggctgactcggacgagagagaaagtttgacaagggaagaggatgaagacagtgaaaaggaggaggaggaggaggataaagagatggaagaattgcaggaagacaaggaatgtgagaacccacaggaggaggaggaggaggaagaggaggaagaagaagaggaggaggaggaagaggaagaggaggcagaggaagctgagcatgaggcggcagcagccaagactgggggtgcagtggaggaggaagcggctcagcaggcaggcagcttccagcagaaggccagcgggagcgagagcaagcgactgtctgaagagaagacaaatgaagcctaggagctcttccaaaaggaagtcctgcttggtaatgaaatttgctctatattacccacgcttttcatggaaacatggctcctgtgctatggttcctgctcactactgtgtaatgtcagaactgaaaaacaaaatactaaaaaaaaaaaaaaaaaaaaaaaaaaaaatccgggtgtgcctgaacctcaaacctagtaatttttcatgcagttttcaaagttaggaacaagtttgtaacatgaagcagattagaaaacaaaggtttctcagcaggttacaggaagctcgatgggcatctggcttggctggcagtattatcactcttacgttgttcattctaagctgtacaatgggagaaattttataattttttattggtaaacatatgctaaatccacttcagtattttattatgttttttttaaatgtgagaacttctgcactacagaattcccttcaccgagcagtagaaagcagttcagaccgtgctggctacctgctgccaccccaccagtcaggaggtaggacctctgaccttagatgccatgctgccatctaacgtgcatcgctagccttaaggaagcagagagaggagcgggagtttcttactcgtgtgatcttaaatggagttcaaaggttgtccttgagttctgactgcagggacggccagtgttgatgtggcgaggacggcagaggcggcagaatcagtgttcgtgattgtgtttgcgtacgtgacagcaactatgaaggatctataggaagcgctgtgtccctttggccttaagcaagacctatgtgctgtaagtgccattgtcagtgtttcaaggctgtaacagccttcactcagtgtgtggcctacaataactagcatttgttgatttgtttgttgtgtcaaaattcccaaataaaactcaaaccactgactctgtcagagaagctgaagcactgggacatttcacacttctgttcctcagtattcatgttacgttgattgactcagaaatctctacagaaataaaagggaaattctccaactacttagtccatgtacttgcgtgtcagacatggataagccattggttcttggctcacagccatttccaaatgttagttattacggacccaggtgatgaacagcagctgatttttacctaccagtattattttatttcttttagtttgtagatctgtgcaacatttttgtactgtacgtcttcaagcctggcagtattaatacccttcttactgacacgtacttttagttttagaaaacttttatatttatgtgtcttatttttatatttctttatttattacacagtgtagtgtataatactgtagtttgtattaatacaataatatattttagtatgaaattttggaaagttgataagatttaaagtagagatgcaattggttctcgtgcattgagatttgatttaacagtgttctgttaacatttatactcgcctcggactgtagaacagagtttaaatgggaatgtattagttttacaactacaatcaagtcattctccctttacccagtttttaatatgaaactcttacactttgaaactcactgtgtgtgactcatagcatgctgctcttctgttaagagttagcccagccataactcattcccttagcaagccaaattagaattgccttctgtaaacagtgttgggaacaatgtttaacatggtgtgccaatttgttcctgtatccatgtatgtaagctaccaatccgactcttccttttaagttccttgttacaccatggtcattttctagttttttaccagactcccagctcacaataaacgcatcaacaagcctga

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