LOCUS       NM_001110810            2732 bp    mRNA    linear   ROD 23-MAR-2023
DEFINITION  Rattus norvegicus myosin light chain kinase 3 (Mylk3), mRNA.
ACCESSION   NM_001110810 XM_001064628 XM_214645
VERSION     NM_001110810.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Avila-Rodriguez D, Solano Agama C, Gonzalez-Pozos S, Vicente
            Mendez-Mendez J, Ortiz Plata A, Arreola-Mendoza L and
            Mendoza-Garrido ME.
  TITLE     The shift in GH3 cell shape and cell motility is dependent on MLCK
            and ROCK
  JOURNAL   Exp Cell Res 354 (1), 1-17 (2017)
   PUBMED   28300565
  REMARK    GeneRIF: We found that a rounded shape with high cortical tension
            depends on MLCK and ROCK, whereas in cells with an elongated shape,
            MLCK is the primary protein responsible for cell spreading.
            Further, in cells with a slow and directionally persistent
            motility, MLCK predominates, while rapid and erratic movement is
            ROCK-dependent
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Eikemo H, Moltzau LR, Hussain RI, Nguyen CH, Qvigstad E, Levy FO,
            Skomedal T and Osnes JB.
  TITLE     CaMKII in addition to MLCK contributes to phosphorylation of
            regulatory light chain in cardiomyocytes
  JOURNAL   Biochem Biophys Res Commun 471 (1), 219-225 (2016)
   PUBMED   26809094
  REMARK    GeneRIF: In conclusion, in addition to MLCK, CaMKII phosphorylates
            RLC in cardiomyocytes.
REFERENCE   3  (bases 1 to 2732)
  AUTHORS   Gao L, Zheng YJ, Gu SS, Tan JL, Paul C, Wang YG and Yang HT.
  TITLE     Degradation of cardiac myosin light chain kinase by matrix
            metalloproteinase-2 contributes to myocardial contractile
            dysfunction during ischemia/reperfusion
  JOURNAL   J Mol Cell Cardiol 77, 102-112 (2014)
   PUBMED   25451385
  REMARK    GeneRIF: Ischemia/reperfusion-activated MMP-2 leads to the
            degradation of cardiac MLCK.
REFERENCE   4  (bases 1 to 2732)
  AUTHORS   Al-Sadi R, Ye D, Dokladny K and Ma TY.
  TITLE     Mechanism of IL-1beta-induced increase in intestinal epithelial
            tight junction permeability
  JOURNAL   J Immunol 180 (8), 5653-5661 (2008)
   PUBMED   18390750
REFERENCE   5  (bases 1 to 2732)
  AUTHORS   Chan JY, Takeda M, Briggs LE, Graham ML, Lu JT, Horikoshi N,
            Weinberg EO, Aoki H, Sato N, Chien KR and Kasahara H.
  TITLE     Identification of cardiac-specific myosin light chain kinase
  JOURNAL   Circ Res 102 (5), 571-580 (2008)
   PUBMED   18202317
REFERENCE   6  (bases 1 to 2732)
  AUTHORS   Seguchi O, Takashima S, Yamazaki S, Asakura M, Asano Y, Shintani Y,
            Wakeno M, Minamino T, Kondo H, Furukawa H, Nakamaru K, Naito A,
            Takahashi T, Ohtsuka T, Kawakami K, Isomura T, Kitamura S, Tomoike
            H, Mochizuki N and Kitakaze M.
  TITLE     A cardiac myosin light chain kinase regulates sarcomere assembly in
            the vertebrate heart
  JOURNAL   J Clin Invest 117 (10), 2812-2824 (2007)
   PUBMED   17885681
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from EU236721.1.
            
            On or before Nov 24, 2007 this sequence version replaced
            XM_001064628.1, XM_214645.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU236721.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760434, SAMN06621351
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q11"
     gene            1..2732
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="myosin light chain kinase 3"
                     /db_xref="GeneID:291926"
                     /db_xref="RGD:1305801"
     exon            1..723
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    43..45
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="upstream in-frame stop codon"
     CDS             238..2598
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /EC_number="2.7.11.18"
                     /function="phosphorylates MLC-2v"
                     /note="cardiac-MLCK; cardiac-MyBP-C-associated Ca/CaM
                     kinase; putative myosin light chain kinase 3"
                     /codon_start=1
                     /product="myosin light chain kinase 3"
                     /protein_id="NP_001104280.1"
                     /db_xref="GeneID:291926"
                     /db_xref="RGD:1305801"
                     /translation="

MSGVSEEDPEGLGPQGLPALGGACLVTVDKKLNVLTEKVDRLLHFQEDVTEKLQCVCQGMDHLEQGLHRLEASQELGLAGPGSTSPAAAQAAWPEVLELVRAVRQEGAQHGARLEALFKMVVAVDRAITLVGSTIQNSKVDDFILQGTVPWRKGSLADGPEENKEQAEVAGVKPKHVLNTGSVQAATSRALWEESQKQDTPVGTVEGLPLIIDTSLKGADLTQAGASLRQGVEALDPGQEPPPAEAESRLPALASEDTGTTLELSVAIDRISEVLTSLRMSQSAGEGTSSSKPDCSEPGPQPLGPLTTDSDIHSDEGLPRISVRMREMTTPEELFETQGGSPIGSAEAPGPGTVLEDQIPKGARPFPPLPKRSCNNGGASAEEATGPGAEPIRGPSLVTRDWRDEPVGTTDLQQGRDPGAVSPEPGKDHAAQGPGRTEAGRRVSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDTLISTSYTVSQHEVLGGGRFGQVHRCTERSTGLALAAKIIKVKNIKDREDVKNEINIMNQLSHVNLIQLYDAFESKNSFTLIMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQTGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETDAETMNFIVNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLNHLIAKASGSNVRLRSQLLLQKYMAQRKWKKHFHVVTAVNRLRKFPTCP"

     misc_feature    700..702
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (E9PT87.1); phosphorylation site"
     misc_feature    934..1011
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="propagated from UniProtKB/Swiss-Prot (E9PT87.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1072..1182
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="propagated from UniProtKB/Swiss-Prot (E9PT87.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1234..1566
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="propagated from UniProtKB/Swiss-Prot (E9PT87.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1258..1260
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (E9PT87.1); phosphorylation site"
     misc_feature    1501..1503
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (E9PT87.1); phosphorylation site"
     misc_feature    1666..2448
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Myosin Light Chain Kinase 3; Region: STKc_MLCK3; cd14192"
                     /db_xref="CDD:271094"
     misc_feature    order(1699..1713,1717..1719,1723..1725,1762..1764,
                     1768..1770,1855..1857,1903..1908,1912..1914,1924..1926,
                     1930..1932,2044..2046,2050..2052,2056..2061,2065..2067,
                     2101..2106,2113..2115,2158..2169)
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="active site"
                     /db_xref="CDD:271094"
     misc_feature    order(1699..1713,1717..1719,1723..1725,1762..1764,
                     1768..1770,1855..1857,1903..1908,1912..1914,1924..1926,
                     2056..2061,2065..2067,2101..2106)
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:271094"
     misc_feature    order(1711..1713,1924..1926,1930..1932,2044..2046,
                     2050..2052,2056..2058,2113..2115,2158..2169)
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]; other site"
                     /db_xref="CDD:271094"
     misc_feature    2101..2169
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="activation loop (A-loop); other site"
                     /db_xref="CDD:271094"
     exon            724..817
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            818..1196
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1600
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1706
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1800
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1910
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2052
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2123
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            2124..2252
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2405
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            2406..2538
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            2539..2732
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

caagggcctgggccaaactcagcctgtcgctctctcagtggttgagtgggagatagacacagagaacttggccctctccgtcccccacacagttcagtcaggaggacaggttggttccctgcagcaaggctcttagcagcggaggacaatggccttccagacgttaccaccaaagtgagaagcagacccttcgtgctccagtttccttgtctttgcccttaccaaaccagaattgggatgtcaggagtttcagaggaggatccagagggtctgggcccccagggtctgccagcgttgggcggagcctgcttagtcaccgtggacaaaaaacttaatgtgctgactgagaaggtcgacagactcttgcatttccaagaagatgtcacagagaagctacagtgtgtgtgccaaggcatggatcacctggaacaaggtctgcatcggctggaggcctcccaggagttgggtctggcagggcccggcagcacttccccagccgctgctcaggccgcatggcctgaggtcctggagctggtgagggccgtgcggcaggagggtgcccagcacggtgccaggctcgaagccctcttcaagatggtggtggctgtggacagggctattactttggtagggtccacaatccagaactccaaagtggatgatttcatcctgcaagggaccgtgccctggaggaaaggcagtctggctgatggccctgaggagaacaaggagcaagcagaagtggctggagtgaagccaaagcatgtgctgaatacaggaagtgtgcaagctgccacttctagggcgctgtgggaagagagccagaagcaggacacacccgtggggacagtggaggggctgcctctcatcatcgatacgtcactgaagggagctgacctcacccaggctggagcctcactgaggcagggagttgaagctcttgacccaggccaagaacccccacccgcagaggcagaatccaggcttcctgcactagccagcgaggacactgggaccaccctggaattgtctgtagcaattgacagaatcagtgaggtcctcactagcctcaggatgtcccaaagtgctggcgaaggaacctcatccagcaagcctgactgttcagagcctggccctcagcccctagggccactaactacagacagtgacattcacagtgatgaaggacttcccaggatctctgtccgtatgcgagagatgactactcctgaggagctgtttgagacccaaggtggcagccccattggctcggcagaagctccaggccctggaactgtgttagaagaccagatccctaaaggagccagaccatttccacccctgccaaagaggagctgcaacaatggtggcgcgagtgcagaggaggcaacagggcctggggctgagcccatcagaggaccaagcttggtcacaagggactggagagatgaacctgttgggaccacagacctgcagcaaggcagagacccaggagcggtgagccctgaacctgggaaggaccatgcagcccagggcccagggagaaccgaagctggaaggagggtgtcttctgctgcagaggctgccatcgtagttctagatgacagcgcagcacccccagccccttttgaacaccgggtagtgagcatcaaagacaccctcatctcgacaagttacacagtgtcccaacacgaagtcttgggagggggccggtttggccaggtgcacaggtgtacagagcggtccacaggccttgcactggcagccaagatcatcaaagtgaagaacataaaggaccgggaggatgtgaagaatgagatcaacatcatgaaccagctcagccacgtaaacttgatccaactttatgatgcttttgagagcaagaacagcttcaccctgatcatggagtatgtggatggaggtgaactcttcgaccggatcacggatgagaagtaccacctgaccgagctggatgtggtcttgttcacaaggcagatctgtgagggtgtgcattacctgcaccagcactacattctgcacctggacctcaagccagagaacatactgtgtgtcagccagactgggcatcaaattaagattattgactttgggctggctagaagatataaacctcgggagaagctaaaggttaactttggtactccagagttcctggctccagaagttgttaactatgagtttgtctcattcccaacagacatgtggagtgtgggagttatcacctacatgctactcagtggtttgtccccatttctaggggaaacagatgcagagaccatgaattttattgtgaactgcagctgggatttcgatgctgataccttcaaagggctgtcagaggaagccaaggactttgtttcacggttgctggtcaaagagaagagttgtaggatgagcgccacacagtgcctgaaacatgagtggttaaatcacctgattgccaaagcctcaggctccaacgttcgcctcagatcccaactactgctgcagaaatatatggctcagcgtaaatggaagaaacatttccatgtggtgactgcagtcaacaggctaagaaaatttccaacgtgtccctaatctacaactgggcctgggagttcctgaggcgacacgcagtggtaatgtgaagagatgactcaggattttatggagtcaggagcttggctgttattgatcttattttgcaaagaatggtggaaggaagaaagaga

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