LOCUS       NM_001109214            2713 bp    mRNA    linear   ROD 21-MAR-2023
DEFINITION  Rattus norvegicus engrailed homeobox 2 (En2), mRNA.
ACCESSION   NM_001109214 XM_001058797 XM_575315
VERSION     NM_001109214.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Sgado P, Alberi L, Gherbassi D, Galasso SL, Ramakers GM, Alavian
            KN, Smidt MP, Dyck RH and Simon HH.
  TITLE     Slow progressive degeneration of nigral dopaminergic neurons in
            postnatal Engrailed mutant mice
  JOURNAL   Proc Natl Acad Sci U S A 103 (41), 15242-15247 (2006)
   PUBMED   17015829
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Simon HH, Scholz C and O'Leary DD.
  TITLE     Engrailed genes control developmental fate of serotonergic and
            noradrenergic neurons in mid- and hindbrain in a gene
            dose-dependent manner
  JOURNAL   Mol Cell Neurosci 28 (1), 96-105 (2005)
   PUBMED   15607945
REFERENCE   3  (bases 1 to 2713)
  AUTHORS   Simon HH, Thuret S and Alberi L.
  TITLE     Midbrain dopaminergic neurons: control of their cell fate by the
            engrailed transcription factors
  JOURNAL   Cell Tissue Res 318 (1), 53-61 (2004)
   PUBMED   15340832
  REMARK    Review article
REFERENCE   4  (bases 1 to 2713)
  AUTHORS   Alberi L, Sgado P and Simon HH.
  TITLE     Engrailed genes are cell-autonomously required to prevent apoptosis
            in mesencephalic dopaminergic neurons
  JOURNAL   Development 131 (13), 3229-3236 (2004)
   PUBMED   15175251
REFERENCE   5  (bases 1 to 2713)
  AUTHORS   Simon HH, Saueressig H, Wurst W, Goulding MD and O'Leary DD.
  TITLE     Fate of midbrain dopaminergic neurons controlled by the engrailed
            genes
  JOURNAL   J Neurosci 21 (9), 3126-3134 (2001)
   PUBMED   11312297
REFERENCE   6  (bases 1 to 2713)
  AUTHORS   Joliot A, Trembleau A, Raposo G, Calvet S, Volovitch M and
            Prochiantz A.
  TITLE     Association of Engrailed homeoproteins with vesicles presenting
            caveolae-like properties
  JOURNAL   Development 124 (10), 1865-1875 (1997)
   PUBMED   9169834
REFERENCE   7  (bases 1 to 2713)
  AUTHORS   Kury P, Schaeren-Wiemers N and Monard D.
  TITLE     Protease nexin-1 is expressed at the mouse met-/mesencephalic
            junction and FGF signaling regulates its promoter activity in
            primary met-/mesencephalic cells
  JOURNAL   Development 124 (6), 1251-1262 (1997)
   PUBMED   9102311
REFERENCE   8  (bases 1 to 2713)
  AUTHORS   Hanks M, Wurst W, Anson-Cartwright L, Auerbach AB and Joyner AL.
  TITLE     Rescue of the En-1 mutant phenotype by replacement of En-1 with
            En-2
  JOURNAL   Science 269 (5224), 679-682 (1995)
   PUBMED   7624797
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH474057.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_575315.2, XM_001058797.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CO400332.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760396
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q11"
     gene            1..2713
                     /gene="En2"
                     /gene_synonym="RGD1561842"
                     /note="engrailed homeobox 2"
                     /db_xref="GeneID:499964"
                     /db_xref="RGD:1561842"
     exon            1..702
                     /gene="En2"
                     /gene_synonym="RGD1561842"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    42..44
                     /gene="En2"
                     /gene_synonym="RGD1561842"
                     /note="upstream in-frame stop codon"
     CDS             48..1019
                     /gene="En2"
                     /gene_synonym="RGD1561842"
                     /note="engrailed 2"
                     /codon_start=1
                     /product="homeobox protein engrailed-2"
                     /protein_id="NP_001102684.1"
                     /db_xref="GeneID:499964"
                     /db_xref="RGD:1561842"
                     /translation="

MEEKDSKSSETAAEAQRQPEPSPGGGSGGGSSPSDSDTGRRRALMLPAVLQAPGNHQHPHRITNFFIDNILRPEFGRRKDAGTCCAGAGGARGGEGGAGSTEGGGGADGAEQILGARESRPNPACAPSAGGPLSGASGDPAADGEGGSKTLSLHGGAKKPGDPGGSLDGALKARGLGGGDLSVSSDSDSSQASATLGAQPMLWPAWVYCTRYSDRPSSGPRSRKPKKKNPNKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQSLAQELSLNESQIKIWFQNKRAKIKKATGNKNTLAVHLMAQGLYNHSTTAKEGKSDSE"

     misc_feature    750..920
                     /gene="En2"
                     /gene_synonym="RGD1561842"
                     /note="Region: Homeodomain; pfam00046"
                     /db_xref="CDD:459649"
     misc_feature    921..1013
                     /gene="En2"
                     /gene_synonym="RGD1561842"
                     /note="Engrailed homeobox C-terminal signature domain;
                     Region: Engrail_1_C_sig; pfam10525"
                     /db_xref="CDD:431338"
     exon            703..2713
                     /gene="En2"
                     /gene_synonym="RGD1561842"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

tttggaggtgggccggtgaagaggcggtttgggattttctgtaaagcatggaggagaaggattccaagtccagcgagacggcggcggaggcgcagcggcagccggaacccagccccggcggcggctccggaggcggcagcagcccgagcgactcggacaccggccgccggcgtgctctgatgttgcccgcggtcctgcaggcgccaggcaaccaccagcatccgcatcgcatcaccaacttcttcatcgataacatcctacgaccggagttcggccgccgaaaggatgcggggacttgctgtgccggcgcgggcggagccaggggaggcgaaggcggcgctggcagtacggagggaggcggcggcgcagacggagccgagcagatcctgggcgccagggagtcccgaccgaaccctgcgtgcgcacccagtgcaggagggccgctctccggtgcctccggcgaccctgcggccgatggagaaggcggctccaagacgctgtcgctgcacggtggtgctaaaaaacccggagacccggggggttccttggatggagcgctcaaggcccggggcttgggcggaggtgacctgtcggtgagctccgactcggacagctctcaagccagcgccaccctgggcgcgcagcccatgctctggccggcttgggtctactgcacacgttactctgaccggccttcttcaggccccaggtctcgaaaaccaaagaagaagaacccgaacaaagaggacaagaggccgcgcaccgccttcactgctgagcagctgcagaggctcaaagctgagtttcagaccaacaggtacctgacggagcagaggcgccaaagtcttgcgcaggagctcagcctcaacgagtcccagatcaagatctggttccagaacaagcgggccaaaatcaagaaagccacgggcaacaagaacactttggcggtgcacctcatggcgcagggcctgtacaaccattccaccaccgccaaggagggcaagtcggacagcgagtagggcgagcagggcgggcgggccgaccggccaggtcttaggaccgcgcaaaacaatgcaataatttaaaaaaccgtgaacaaagggccagtgtataaagattataccagcattatctgtgaaaaacccgtgtattagctatggttctacaataatctatgtacatataatttacagatggtgtataatccaaaaaaactgactataaaatatttttgaggttttttttgtgcttaagaggttatgctaatttgttattatttttctgcaaagggggctgtttcttttctttttcttttcccccttgtttaatctcctaagctccactttgggacattggacaccctcccccccccccgaaagaaaaaaaattaaaacaacttgctgaagtccaaagatttttattgctgcatttcacaggactgtgaaccaataaatagctcctatttggtctgtgggctctgccgcttgctttgtgctggctcggacagcgctgtaggaagcgccagcgtggtgatggagagctctggtggggagagagggcacagttcttttgctgaggtctgaggaagtccattcaggccatcgatcctcccagcccctctccacttctgcctcctactgcctcacttcccaaccaccgcagaggcccttgctggcagccaccgagtgtctgcgtctgtgtctatgacccctgtccccgccacagtcccgtagcgactgccagcgccaactctcccgcggctgtgggcaggcagcgcttggggcctggggagcagtgcgagtgtaccctgaaggcttgaaggctgctaggacagctcgccctgatccaaaagcccccgccgaggtgggtcttggggcctggcagagcaagttggcatcgtcttccctttccttttcttcctctttttttctttttcacttatttttgaatggtacatacatgtttgtgtacatacttgtgtatgcatgtgcacgcacaccccaggcctgtgtgaagctggggttctggagctcgaagctaccttgactttcaaaggaactcgaatcctcctgaatctaaaggacatgtgtaaataatcatttcttatcacgttttgtcttttttgtttttttaaagaaaatatacattttatttttgaaggtgtggtactgtgtaaattaaatatattcaatatattcctcaccaagtacatatatatataagcaaacacattatatataactccacaatgtcttctgtttagtgtatgggaaaaaggtgtccaaatgtccacctggggccagcacagtggcaagggtgttctcagctgagctgagcgtcaggctgcctgctgaggactgatggtggatttctctctttgccttaaacctctttatttcactgcgccaacactttctctttgtacatattcgtgccaaccttctgaaaaggaagctataaaaacagaagttgtaatttaagagtgtgaataaccatcttctgcttggggatcggtgaaacacatgccttttagcaggaagcaaatctgtccttttttaagtttataaaatgtgcattttacagtatcaaatatttataatcttatttgaaatggatgaatgttccctacttacaactgtggttatcaaaattgtgaccattccaacctgtgcatttttgtgtgtttaaattcctgaaagttacactaaataaa

//