LOCUS       NM_001106774            2864 bp    mRNA    linear   ROD 28-DEC-2023
DEFINITION  Rattus norvegicus NUAK family kinase 1 (Nuak1), mRNA.
ACCESSION   NM_001106774 XM_001079579 XM_234998
VERSION     NM_001106774.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Mudunuru J, Ren C, Taft DR and Maniar M.
  TITLE     Effect of Gender on the Pharmacokinetics of ON 123300, A Dual
            Inhibitor of ARK5 and CDK4/6 for the Treatment of Cancer, in Rats
  JOURNAL   Eur J Drug Metab Pharmacokinet 44 (4), 531-538 (2019)
   PUBMED   30701428
  REMARK    GeneRIF: A small molecule dual inhibitor of the c-MYC activated
            kinases ARK5 and CDK4/6, is being developed.
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Esteve-Puig R, Gil R, Gonzalez-Sanchez E, Bech-Serra JJ, Grueso J,
            Hernandez-Losa J, Moline T, Canals F, Ferrer B, Cortes J, Bastian
            B, Ramon Y Cajal S, Martin-Caballero J, Flores JM, Vivancos A,
            Garcia-Patos V and Recio JA.
  TITLE     A mouse model uncovers LKB1 as an UVB-induced DNA damage sensor
            mediating CDKN1A (p21WAF1/CIP1) degradation
  JOURNAL   PLoS Genet 10 (10), e1004721 (2014)
   PUBMED   25329316
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2864)
  AUTHORS   Maekawa R, Sato S, Yamagata Y, Asada H, Tamura I, Lee L, Okada M,
            Tamura H, Takaki E, Nakai A and Sugino N.
  TITLE     Genome-wide DNA methylation analysis reveals a potential mechanism
            for the pathogenesis and development of uterine leiomyomas
  JOURNAL   PLoS One 8 (6), e66632 (2013)
   PUBMED   23818951
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2864)
  AUTHORS   Hou X, Liu JE, Liu W, Liu CY, Liu ZY and Sun ZY.
  TITLE     A new role of NUAK1: directly phosphorylating p53 and regulating
            cell proliferation
  JOURNAL   Oncogene 30 (26), 2933-2942 (2011)
   PUBMED   21317932
REFERENCE   5  (bases 1 to 2864)
  AUTHORS   Zagorska A, Deak M, Campbell DG, Banerjee S, Hirano M, Aizawa S,
            Prescott AR and Alessi DR.
  TITLE     New roles for the LKB1-NUAK pathway in controlling myosin
            phosphatase complexes and cell adhesion
  JOURNAL   Sci Signal 3 (115), ra25 (2010)
   PUBMED   20354225
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2864)
  AUTHORS   Humbert N, Navaratnam N, Augert A, Da Costa M, Martien S, Wang J,
            Martinez D, Abbadie C, Carling D, de Launoit Y, Gil J and Bernard
            D.
  TITLE     Regulation of ploidy and senescence by the AMPK-related kinase
            NUAK1
  JOURNAL   EMBO J 29 (2), 376-386 (2010)
   PUBMED   19927127
REFERENCE   7  (bases 1 to 2864)
  AUTHORS   Al-Hakim AK, Zagorska A, Chapman L, Deak M, Peggie M and Alessi DR.
  TITLE     Control of AMPK-related kinases by USP9X and atypical
            Lys(29)/Lys(33)-linked polyubiquitin chains
  JOURNAL   Biochem J 411 (2), 249-260 (2008)
   PUBMED   18254724
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473960.2.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_001079579.1, XM_234998.4.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132291,
                              SAMD00132294 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q13"
     gene            1..2864
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /note="NUAK family kinase 1"
                     /db_xref="GeneID:299694"
                     /db_xref="RGD:1309956"
     exon            1..396
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /inference="alignment:Splign:2.1.0"
     CDS             154..2139
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /EC_number="2.7.11.1"
                     /note="AMPK-related protein kinase 5; NUAK family,
                     SNF1-like kinase, 1"
                     /codon_start=1
                     /product="NUAK family SNF1-like kinase 1"
                     /protein_id="NP_001100244.1"
                     /db_xref="GeneID:299694"
                     /db_xref="RGD:1309956"
                     /translation="

MEGAAVSAAGDGPAVETGLPGSPREVVAGATAAPVEPRKPHGVKRHHHKHNLKHRYELQETLGKGTYGKVKRATERFSGRVVAIKSIRKDKIKDELDMVHIRREIEIMSSLNHPHIISIYEVFENKDKIVIIMEYASKGELYDYISERRRLSERETRHFFRQIVSAVHYCHKNGVVHRDLKLENILLDDNCNIKIADFGLSNLYQKDKFLQTFCGSPLYASPEIVNGRPYRGPEVDSWALGVLLYTLIYGTMPFDGFDHKNLIRQISSGEYREPTQPSDARGLIRWMLMVNPDRRATIEDIANHWWVNWGYKSSVCDCDALPDSESPLLARIIDWHHRSTGLQAEADAKMKAKPGASEVVLERQRSLKKSKKENDFPQSGQDSLPESPSKLSSKRPKGILKKRSNSEHRSHSTGFIEGIVGPALPSPFKMEQDLCRTAIPLPGSPEAQGSSGKLSLKQSATMPKKGILKKTQQRESGYYSSPERSESSELLDSNNVIISSGLSSPPPDPARGTSHSLSCRRKGILKHSSRYSDGATDPALTGPEIPTLESLSPPGVPSDGISRSYSRPSSIISDDSVLSSDSFDLLDLQENRPARQRIRSCVSAENFLQLQDFETPHNRPRPQYLKRYRNRLADSSFSLLADMDDVTQVYKKALEICSKLN"

     misc_feature    313..1074
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     novel (nua) kinase family NUAK; Region: STKc_NUAK;
                     cd14073"
                     /db_xref="CDD:270975"
     misc_feature    order(337..351,361..363,400..402,406..408,502..504,
                     550..561,571..573,577..582,586..588,685..690,694..696,
                     700..705,709..711,739..744,751..753,790..807,811..813,
                     901..912,916..918)
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /note="active site"
                     /db_xref="CDD:270975"
     misc_feature    order(337..348,361..363,400..402,406..408,502..504,
                     550..561,571..573,700..705,709..711,739..744)
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:270975"
     misc_feature    order(571..573,577..582,586..588,688..690,694..696,
                     700..702,751..753,790..807,811..813,901..912,916..918)
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]; other site"
                     /db_xref="CDD:270975"
     misc_feature    739..807
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /note="activation loop (A-loop); other site"
                     /db_xref="CDD:270975"
     exon            397..517
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /inference="alignment:Splign:2.1.0"
     exon            518..669
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /inference="alignment:Splign:2.1.0"
     exon            670..735
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /inference="alignment:Splign:2.1.0"
     exon            736..855
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /inference="alignment:Splign:2.1.0"
     exon            856..988
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /inference="alignment:Splign:2.1.0"
     exon            989..2864
                     /gene="Nuak1"
                     /gene_synonym="RGD1309956"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

tgccccggcggcagccagagccaggtcgggtcgtctcagcaatgctgctgcgggggcgaccttgaacgcaccccggtttccctcggcagggaccaccccgagcccccgtgccgggtccagccctctccagctcggcttgcctggcgcttggtcatggaaggggcggcagtgtccgcggcaggggatggccctgctgtggagacgggtttgcccggctctccgcgggaggtagtggccggggcgactgctgctccagtggaacccaggaagccgcacggggtgaagcgacatcaccacaaacacaacttgaagcatcgctatgagctgcaagagaccctgggcaaaggtacctacggcaaagtcaagagagccacggagaggttttcaggccgagtggttgctataaaatccatccgtaaggacaaaatcaaggatgagctagacatggttcacatcagacgagagattgagattatgtcatccctcaaccatcctcatatcatcagtatttatgaagtgtttgagaacaaagataagattgtgatcatcatggaatatgccagcaaaggagagctgtatgactacatcagtgagcggagacgcctcagcgagagggagaccaggcacttcttccggcagatcgtgtctgccgtgcattactgtcacaagaatggcgtggtccatcgggacttgaagctggagaacatactgcttgatgacaactgtaatattaagatcgctgactttgggctttccaacctgtaccagaaggacaagttcttgcaaacattttgtgggagcccactctatgcatcacccgagattgtcaatggcaggccttaccgggggccagaggtggacagctgggccctgggcgtcttgctttacactctcatttatggaacaatgcccttcgatggctttgatcacaaaaacctcatccgacagatcagcagtggagagtaccgggagcccacacaaccctcagatgctcggggactcatccggtggatgctgatggtgaatcctgaccgccgggccaccatcgaggacatcgccaaccactggtgggtgaactggggctacaagagcagcgtctgtgactgcgatgcccttcccgactccgagtctccgctcttggctcggatcattgattggcatcaccgctccaccgggctgcaggctgaggctgacgccaagatgaaggccaagcccggggcctcggaggttgtgttggagcggcaacggtcactgaagaagtccaagaaagagaacgactttccccagtctggccaggactcattgcctgaaagcccatccaagctgagttccaagaggcccaagggcatcctgaagaagcggagcaacagtgaacatcgctcccacagcactggcttcatcgaaggcatcgtgggccccgccttaccatcccctttcaaaatggagcaagacttgtgccggactgccatccccctccccggctcccccgaggcgcagggaagctcagggaagctcagcctcaaacagtctgccaccatgcccaagaaaggcatcttgaaaaagacccagcagagagaatctggttactactcgtccccagagcgcagcgagtcctcagagctgctggacagtaacaatgtgatcatcagcagtggcctctcctcccctcccccagacccagccaggggaacgtcccacagcctgtcctgccggaggaagggcatcttgaaacacagcagcaggtactcggatggtgccacagaccctgccctcaccggccccgaaatacccacactggaatccttgtcgcctcccggtgtcccctccgatggcatctctcggagctacagtcgcccgtccagtatcatcagtgacgacagcgtcctgtccagcgactctttcgacttgttagatctccaggagaatcggccggcccgccagcgcatccgcagctgcgtctctgcggaaaacttcctccagcttcaggactttgagactccccacaaccggccccggccccagtacctgaagcggtaccggaaccggctggcagacagtagcttctcccttcttgctgatatggacgacgtgactcaggtgtataagaaggctctggagatctgcagcaagctcaactagccctccgaggctgggctgggtgggtgggagagcccaagggacgaggggaacagaactcagcctgcaggctggttacctctttgctggtcatggcaaccgactgggaaaagactagtaacgtctggcccccggccagctttcgcagccttgagtcagcacctaaaggggacggaaactgttctttaccagttctgccagccgccagtcagaacatgggtcctaactggcattcccatatggcatctcacagggaaggtggttgttggccagcctgaaacgggaaggggcattatgggtaaggggggcgggtttcctagaagtcatctaggtgtttctggaggagggcaagaaagactgagaccatctgccttgggtgaataggttagcgagcctggtctggctatgtaggggctagcaagatgccagcgacctgagtgtgaattggggatcaactggtaatgtccgcactgcggtctgaatttagtggaccaggcacaccagggagcagtgatcgagtaccagtaactgccaggggatcaggaatgggggcaacagcatgacctgccattgctaggacctgggacttaggtgaaagcaccctgtttaaaagaaaaaaggctaactctttcctggcacagcatgacatgttaatttgctcctggactttgagtagaaagcaaatgcattgaactctgataggggaatagtctggtggcggttaactt

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