LOCUS       NM_001401875            1817 bp    mRNA    linear   PLN 22-NOV-2023
DEFINITION  Oryza sativa Japonica Group probable L-ascorbate peroxidase 8,
            chloroplastic (LOC4329643), transcript variant 2, mRNA; nuclear
            gene for chloroplast product.
ACCESSION   NM_001401875 XM_015767621
VERSION     NM_001401875.1
KEYWORDS    RefSeq.
SOURCE      Oryza sativa Japonica Group (Japanese rice)
  ORGANISM  Oryza sativa Japonica Group
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Jiang G, Yin D, Zhao J, Chen H, Guo L, Zhu L and Zhai W.
  TITLE     The rice thylakoid membrane-bound ascorbate peroxidase OsAPX8
            functions in tolerance to bacterial blight
  JOURNAL   Sci Rep 6, 26104 (2016)
   PUBMED   27185545
  REMARK    GeneRIF: The overexpressing lines exhibited increased tolerance to
            bacterial blight. The RNA interference (RNAi) lines were
            considerably more sensitive than the control plant.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Kikuchi S, Satoh K, Nagata T, Kawagashira N, Doi K, Kishimoto N,
            Yazaki J, Ishikawa M, Yamada H, Ooka H, Hotta I, Kojima K, Namiki
            T, Ohneda E, Yahagi W, Suzuki K, Li CJ, Ohtsuki K, Shishiki T,
            Otomo Y, Murakami K, Iida Y, Sugano S, Fujimura T, Suzuki Y,
            Tsunoda Y, Kurosaki T, Kodama T, Masuda H, Kobayashi M, Xie Q, Lu
            M, Narikawa R, Sugiyama A, Mizuno K, Yokomizo S, Niikura J, Ikeda
            R, Ishibiki J, Kawamata M, Yoshimura A, Miura J, Kusumegi T, Oka M,
            Ryu R, Ueda M, Matsubara K, Kawai J, Carninci P, Adachi J, Aizawa
            K, Arakawa T, Fukuda S, Hara A, Hashizume W, Hayatsu N, Imotani K,
            Ishii Y, Itoh M, Kagawa I, Kondo S, Konno H, Miyazaki A, Osato N,
            Ota Y, Saito R, Sasaki D, Sato K, Shibata K, Shinagawa A, Shiraki
            T, Yoshino M, Hayashizaki Y and Yasunishi A.
  CONSRTM   Rice Full-Length cDNA Consortium; National Institute of
            Agrobiological Sciences Rice Full-Length cDNA Project Team;
            Foundation of Advancement of International Science Genome
            Sequencing & Analysis Group; RIKEN
  TITLE     Collection, mapping, and annotation of over 28,000 cDNA clones from
            japonica rice
  JOURNAL   Science 301 (5631), 376-379 (2003)
   PUBMED   12869764
  REMARK    Erratum:[Science. 2003 Sep;301(5641):1849]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB114856.1, CI581719.1 and CI408052.1.
            
            On Mar 1, 2022 this sequence version replaced XM_015767621.2.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN03003175,
                              SAMN03003383 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               AB114856.1         1-201
            202-655             AB114856.1         205-658
            656-658             CI581719.1         167-169
            659-1648            AB114856.1         662-1651
            1649-1817           CI408052.1         258-426
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Oryza sativa Japonica Group"
                     /mol_type="mRNA"
                     /db_xref="taxon:39947"
                     /chromosome="2"
                     /map="2"
     gene            1..1817
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="probable L-ascorbate peroxidase 8, chloroplastic"
                     /db_xref="GeneID:4329643"
     exon            1..201
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..1464
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /EC_number="1.11.1.11"
                     /note="isoform 2 is encoded by transcript variant 2;
                     putative thylakoid-bound ascorbate peroxidase;
                     LOC_Os02g34810"
                     /codon_start=1
                     /product="probable L-ascorbate peroxidase 8, chloroplastic
                     isoform 2"
                     /protein_id="NP_001388804.1"
                     /db_xref="GeneID:4329643"
                     /translation="

MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAGGLRLRSRPSRFPQAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREILKTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLIQPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGPRVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQSWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTPVPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSPDKPLQSNYFLNIMLLIGGLAFLTSLLGS"

     misc_feature    31..123
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q69SV0.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     mat_peptide     256..1461
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /product="Probable L-ascorbate peroxidase 8,
                     chloroplastic. /id=PRO_0000042658"
                     /note="propagated from UniProtKB/Swiss-Prot (Q69SV0.2)"
     misc_feature    295..1074
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="Ascorbate peroxidases and cytochrome C peroxidases;
                     Region: ascorbate_peroxidase; cd00691"
                     /db_xref="CDD:173825"
     misc_feature    order(352..357,364..366,373..375,667..675,712..714,
                     751..756,760..765,769..783,790..795,859..861,937..939,
                     943..945,1027..1029,1039..1041)
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="heme binding site [chemical binding]; other site"
                     /db_xref="CDD:173825"
     misc_feature    364..366
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="Transition state stabilizer.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; propagated
                     from UniProtKB/Swiss-Prot (Q69SV0.2); other site"
     misc_feature    order(595..597,763..765,769..774,778..780,901..903,
                     928..933)
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="substrate binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:173825"
     misc_feature    760..855
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q69SV0.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    order(766..768,862..864,868..870,877..879,883..885,
                     889..891)
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="K+ binding site [ion binding]; other site"
                     /db_xref="CDD:173825"
     misc_feature    1063..1278
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q69SV0.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1399..1461
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q69SV0.2);
                     transmembrane region"
     exon            202..360
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            361..481
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            482..582
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            583..670
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            671..738
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            739..825
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            826..900
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            901..978
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1083
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1311
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1817
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgactacattctctatactgagggaaattatgacattctctaaactaaggagggaaataatgaggacaccccatatatcactctttcttatggcatgttcagtataaaaggaaagtgcattttcctc

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