LOCUS       NM_001401874            1820 bp    mRNA    linear   PLN 22-NOV-2023
DEFINITION  Oryza sativa Japonica Group probable L-ascorbate peroxidase 8,
            chloroplastic (LOC4329643), transcript variant 1, mRNA; nuclear
            gene for chloroplast product.
ACCESSION   NM_001401874 XM_015767619
VERSION     NM_001401874.1
KEYWORDS    RefSeq.
SOURCE      Oryza sativa Japonica Group (Japanese rice)
  ORGANISM  Oryza sativa Japonica Group
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Jiang G, Yin D, Zhao J, Chen H, Guo L, Zhu L and Zhai W.
  TITLE     The rice thylakoid membrane-bound ascorbate peroxidase OsAPX8
            functions in tolerance to bacterial blight
  JOURNAL   Sci Rep 6, 26104 (2016)
   PUBMED   27185545
  REMARK    GeneRIF: The overexpressing lines exhibited increased tolerance to
            bacterial blight. The RNA interference (RNAi) lines were
            considerably more sensitive than the control plant.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Kikuchi S, Satoh K, Nagata T, Kawagashira N, Doi K, Kishimoto N,
            Yazaki J, Ishikawa M, Yamada H, Ooka H, Hotta I, Kojima K, Namiki
            T, Ohneda E, Yahagi W, Suzuki K, Li CJ, Ohtsuki K, Shishiki T,
            Otomo Y, Murakami K, Iida Y, Sugano S, Fujimura T, Suzuki Y,
            Tsunoda Y, Kurosaki T, Kodama T, Masuda H, Kobayashi M, Xie Q, Lu
            M, Narikawa R, Sugiyama A, Mizuno K, Yokomizo S, Niikura J, Ikeda
            R, Ishibiki J, Kawamata M, Yoshimura A, Miura J, Kusumegi T, Oka M,
            Ryu R, Ueda M, Matsubara K, Kawai J, Carninci P, Adachi J, Aizawa
            K, Arakawa T, Fukuda S, Hara A, Hashizume W, Hayatsu N, Imotani K,
            Ishii Y, Itoh M, Kagawa I, Kondo S, Konno H, Miyazaki A, Osato N,
            Ota Y, Saito R, Sasaki D, Sato K, Shibata K, Shinagawa A, Shiraki
            T, Yoshino M, Hayashizaki Y and Yasunishi A.
  CONSRTM   Rice Full-Length cDNA Consortium; National Institute of
            Agrobiological Sciences Rice Full-Length cDNA Project Team;
            Foundation of Advancement of International Science Genome
            Sequencing & Analysis Group; RIKEN
  TITLE     Collection, mapping, and annotation of over 28,000 cDNA clones from
            japonica rice
  JOURNAL   Science 301 (5631), 376-379 (2003)
   PUBMED   12869764
  REMARK    Erratum:[Science. 2003 Sep;301(5641):1849]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB114856.1, CI581719.1 and CI408052.1.
            
            On Mar 3, 2022 this sequence version replaced XM_015767619.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB114856.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03003175, SAMN03003383
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-658               AB114856.1         1-658
            659-661             CI581719.1         167-169
            662-1651            AB114856.1         662-1651
            1652-1820           CI408052.1         258-426
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Oryza sativa Japonica Group"
                     /mol_type="mRNA"
                     /db_xref="taxon:39947"
                     /chromosome="2"
                     /map="2"
     gene            1..1820
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="probable L-ascorbate peroxidase 8, chloroplastic"
                     /db_xref="GeneID:4329643"
     exon            1..201
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..1467
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /EC_number="1.11.1.11"
                     /note="isoform 1 is encoded by transcript variant 1;
                     putative thylakoid-bound ascorbate peroxidase;
                     LOC_Os02g34810"
                     /codon_start=1
                     /product="probable L-ascorbate peroxidase 8, chloroplastic
                     isoform 1"
                     /protein_id="NP_001388803.1"
                     /db_xref="GeneID:4329643"
                     /translation="

MAERIAASLLPAASPSPAPSPPPPRPRVSAAAAASFPCCSTSAGGLRLRSRPSRFPQKAATTRSGRAGAGARAVVRCMAAAAVAASDAAQLKSAREDIREILKTTYCHPIMVRLGWHDSGTYDKNIEEWPQRGGADGSLRFDAELSHGANAGLINALKLIQPIKDKYPGITYADLFQLASATAIEEAGGPKIPMKYGRVDVTAAEQCPPEGRLPDAGPRVPADHLREVFYRMGLDDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGEPGGQSWTVEWLKFDNSYFKDIKEQRDQDLLVLPTDAALFEDPSFKVYAEKYAEDQEAFFKDYAEAHAKLSDLGAKFDPPEGFSLDDEPAVEEKDPEPAPAPAAAPPPPPVEEKKEAEPTPVPVTVGAAVASSPADDNNGAAPQPEPFVAAKYSYGKKELSDSMKQKIRAEYEGFGGSPDKPLQSNYFLNIMLLIGGLAFLTSLLGS"

     transit_peptide 31..258
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="Chloroplast. /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q69SV0.2)"
     misc_feature    31..123
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q69SV0.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    160..228
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q69SV0.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     mat_peptide     259..1464
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /product="Probable L-ascorbate peroxidase 8,
                     chloroplastic. /id=PRO_0000042658"
                     /note="propagated from UniProtKB/Swiss-Prot (Q69SV0.2)"
     misc_feature    298..1077
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="Ascorbate peroxidases and cytochrome C peroxidases;
                     Region: ascorbate_peroxidase; cd00691"
                     /db_xref="CDD:173825"
     misc_feature    order(355..360,367..369,376..378,670..678,715..717,
                     754..759,763..768,772..786,793..798,862..864,940..942,
                     946..948,1030..1032,1042..1044)
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="heme binding site [chemical binding]; other site"
                     /db_xref="CDD:173825"
     misc_feature    367..369
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="Transition state stabilizer.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; propagated
                     from UniProtKB/Swiss-Prot (Q69SV0.2); other site"
     misc_feature    order(598..600,766..768,772..777,781..783,904..906,
                     931..936)
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="substrate binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:173825"
     misc_feature    763..858
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q69SV0.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    order(769..771,865..867,871..873,880..882,886..888,
                     892..894)
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="K+ binding site [ion binding]; other site"
                     /db_xref="CDD:173825"
     misc_feature    1066..1281
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q69SV0.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1402..1464
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q69SV0.2);
                     transmembrane region"
     exon            202..363
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            364..484
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            485..585
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            586..673
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            674..741
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            742..828
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            829..903
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            904..981
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1086
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1314
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1820
                     /gene="LOC4329643"
                     /gene_synonym="APX8; OsAPx08; OsAPx8; P0470G10.5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

aaaaactcactcgactcgagcgcgcgcgccatggcggagcgcatcgccgcctccctcctcccggctgcctcgccctcgcctgctccgtcccctccccccccgcgcccccgcgtctccgccgcggccgccgcctccttcccatgctgctccaccagcgccggcggcctccgcctccgctcccgcccgtctcgcttcccgcagaaggctgcgacgacgaggagcgggcgcgccggcgcgggggcgcgggcggtggtccggtgcatggcggcggcggcggtggcggcgtccgacgcggcgcagctcaagagcgcccgggaggacatcagggagatcctcaagaccacctactgccaccccatcatggtccgtcttgggtggcacgattctggcacgtacgacaagaacatcgaggagtggccgcagaggggcggagccgacgggagcttgagatttgacgccgaattgagccacggagccaatgctggtctgattaatgctttgaagcttatccaaccaatcaaggacaaatacccgggtataacttatgctgatttgttccagttggcaagtgctacagcaattgaggaagctggtgggccgaaaattccaatgaaatatggacgagttgatgtcacagcagctgagcagtgcccaccagaggggaggcttcctgatgccggtccacgtgtgcccgctgatcatcttagggaggtattctacaggatgggccttgatgacaaggaaattgttgcattatctggagcacacacacttggaagatcaagacctgacaggagtggctggggaaagccagaaacaaaatatactaaggatgggcctggtgaacctggagggcaatcatggacagttgaatggttgaagtttgataacagttacttcaaggacataaaagagcaaagggaccaggatcttctagtgctacccacagatgctgcattatttgaggatccgtccttcaaggtatatgccgaaaaatatgcagaggatcaggaggcattctttaaagactacgctgaagctcatgctaaactgagcgaccttggtgcaaagttcgatccacctgagggattttcactggacgatgaaccagccgtcgaagagaaggatcctgaaccagcaccagcgccagcagcagcaccaccacctccaccagtcgaggagaagaaggaagctgaaccaactccagtaccagtaacggtaggagcagcagtggcatcatcgccagcggatgacaacaacggtgcagcaccgcaaccagagcccttcgtcgctgcgaaatactcctacggaaagaaggagctgtcggactcgatgaagcagaagatcagggcggagtacgagggattcggaggcagcccggacaagcctctgcagtccaactacttcctcaacatcatgctcttgatcggagggctggccttcttgacgtctctgctcgggagctgagagcgatggtctgatgacctcctctgacgagtgttttgagttgttctgcctgtgctaagatttgcgtgtttctctttccatgttttgagtcgttattccgtaaataaattgaggtaaaaggatgggcatgtgaatggattccagtactttctgaacttcttgagtaattcctctagagaaatatttgtcggagagaaatgtcacattatctagttgtcatggctcatgactacattctctatactgagggaaattatgacattctctaaactaaggagggaaataatgaggacaccccatatatcactctttcttatggcatgttcagtataaaaggaaagtgcattttcctc

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