LOCUS       XM_036164537            2850 bp    mRNA    linear   ROD 10-APR-2023
DEFINITION  PREDICTED: Mus musculus argonaute RISC catalytic subunit 4 (Ago4),
            transcript variant X1, mRNA.
ACCESSION   XM_036164537
VERSION     XM_036164537.1
DBLINK      BioProject: PRJNA169
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_000070.7) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_000001635.27-RS_2023_04
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           RefSeqFE; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 04/05/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /chromosome="4"
     gene            1..2850
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="argonaute RISC catalytic subunit 4; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 6 ESTs, 40 long SRA reads, 1 Protein, and 100%
                     coverage of the annotated genomic feature by RNAseq
                     alignments, including 19 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:76850"
                     /db_xref="MGI:MGI:1924100"
     CDS             384..2750
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X1"
                     /protein_id="XP_036020430.1"
                     /db_xref="GeneID:76850"
                     /db_xref="MGI:MGI:1924100"
                     /translation="

MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRIDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKHPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPSQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKMNAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEITQELLYSQEVVQDLTSMARELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKMERVGKSGNVPAGTTVDSTVTHPSEFDFYLCSHAGIQEAKSCWKCIHPKTLPPGNQPSFTLPGLVG"

     misc_feature    459..848
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    879..1031
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    1032..1394
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1188..1190,1233..1235,1275..1277,1287..1289,
                     1341..1343,1362..1364,1368..1370)
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1533..2660
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1944..1946,1956..1958,1992..2003,2010..2012,
                     2034..2036,2043..2045,2055..2057,2067..2069)
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
ORIGIN      

cccctgcctcggtcaggctcgctggaccccgcgcgtgcggggccgcgcacgcccccttcgctagccgggacccgggaccgcaggcgcctggcgcccccgccccgcccggcgggcgcgcgccgcctcccgctcccgttccagctggcggcggcggcggcagcggccgggattgtttttgttgtcgctgaggccggaagagccgtgacagagtcgggtccctgtccccggactgggcgccgccgccgccccctgcccgcccccggcccgcgtcttcgcggcgccgcttcagcgccaatattccagagagcaagcgttacgcggctccggcggggcccggagcgggaggcgctggggaccggagcgaggcggcccccgtcgccgccatggaggcgctgggacccggacctccagccagcctcttccagccacctcggcgtcctggccttggaaccgttgggaaaccaattcgactgttagccaatcattttcaggttcagatccctaaaatagatgtgtatcactatgatgtggatattaaaccagaaaaacggcctcgtagagtcaacagggaggtggtagacactatggtgcggcacttcaagatgcagatatttggagatcggcagcctggctacgatggcaaaagaaacatgtacacggcacacccactgccaatcggacgggataggattgatatggaggtgaccctcccaggtgagggtaaagaccaaactttcaaagtgtctgtgcagtgggtgtcagttgtgagccttcagctgcttttagaagctttagcaggacacctgaatgaagtcccagatgactcagtacaagcacttgatgtgattacaaggcatctcccctctatgaggtacactccagtaggccggtcttttttctcacctcctgaaggttattaccaccctctgggagggggcagagaggtttggtttggctttcatcagtctgtgagacctgccatgtggaatatgatgctcaatattgatgtatctgcaactgctttctaccgggctcaacctatcattgagttcatgtgtgaggttttagacattcagaacatcaatgagcagacaaaacctctaacagactcccagcgtgtcaagtttaccaaagaaattaggggtcttaaggttgaggtgactcactgtggacagatgaaacgaaaatatcgagtttgtaatgtgacaagacggccagccagtcatcaaaccttccctttgcagctagagaatggccaagctatggaatgtacagtggctcagtattttaagcagaagtatagtctgcagctgaaacacccccaccttccctgccttcaagtggggcaagagcaaaagcatacctacctaccacttgaggtctgtaatatagtggcaggacagagatgtataaagaagctcacagacaatcagacatccaccatgatcaaagccacggcaagatctgctcctgacagacaggaggaaatcagtagactggtgaagagcaacagcatggtgggtggacctgacccatacctgaaggaatttgggattgttgtgcacaacgagatgacagagctcacaggcagggtgcttccggctccaatgctgcagtatggaggccggaataaaacagtagccacacccagccagggtgtctgggacatgcgaggaaaacagttttatgctggcattgaaattaaagtatgggcagtggcttgttttgcgcctcagaaacaatgtagggaagatttgctaaagagtttcactgaccaacttcgtaaaatctccaaggatgcagggatgcccatccagggtcagccatgtttctgcaagtatgctcaaggtgcagacagcgtggagcccatgtttaaacatctgaaaatgacatatgtgggcctgcagctaatagtggtgattttgcctgggaagacaccagtatatgcggaggtgaagcgtgttggagatacccttctaggtatggccacacagtgtgtccaggtcaaaaacgttgtgaagacctcgccccagaccctttccaacctttgcctgaagatgaacgcaaagctgggagggattaacaatgtgcttgtgcctcaccaaaggccctcagtgttccagcagcctgtcatctttctgggagcagatgtcactcatccgcctgctggggacgggaagaagccttctattgcagccgtggtgggcagcatggacggccatcccagccggtactgtgccacagtccgggtacagacatctcgacaggagatcacccaggagctcctctacagccaggaggtcgtgcaggacctgacgagcatggctcgggagctgctgattcaattctataagtccacgcgcttcaagcccacacgcatcatctactaccgcggaggggtatccgagggacagatgaagcaggtagcttggccggagctaatagcaattcgaaaggcatgtataagcttggaggaagactatcggccgggaataacttacattgtggtgcaaaagaggcaccacacacgactcttctgtgcagataaaatggaaagggtggggaaaagtggcaatgtcccagcaggcaccacagtggatagcaccgtcacacacccgtctgagtttgacttttacctctgtagtcatgcaggaattcaggaagccaagtcctgctggaaatgcattcatcctaaaactctccctccagggaaccagccgtccttcacattaccaggtcttgtgggatgacaactgcttcactgcagatgaactccagctactgacttaccagctctgtcacacctacgtgaggtgcacacgctccgtctccattcctgcccctgcatat

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