LOCUS       XM_030253389           15824 bp    mRNA    linear   ROD 10-APR-2023
DEFINITION  PREDICTED: Mus musculus argonaute RISC catalytic subunit 3 (Ago3),
            transcript variant X7, mRNA.
ACCESSION   XM_030253389
VERSION     XM_030253389.2
DBLINK      BioProject: PRJNA169
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_000070.7) annotated using gene prediction method: Gnomon,
            supported by mRNA and EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 21, 2020 this sequence version replaced XM_030253389.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_000001635.27-RS_2023_04
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           RefSeqFE; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 04/05/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15824
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /chromosome="4"
     gene            1..15824
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="argonaute RISC catalytic subunit 3; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 8 mRNAs, 21 ESTs, 685 long SRA reads, 5 Proteins, and
                     100% coverage of the annotated genomic feature by RNAseq
                     alignments, including 17 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:214150"
                     /db_xref="MGI:MGI:2446634"
     CDS             1325..3082
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /codon_start=1
                     /product="protein argonaute-3 isoform X4"
                     /protein_id="XP_030109249.1"
                     /db_xref="GeneID:214150"
                     /db_xref="MGI:MGI:2446634"
                     /translation="

MRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNFFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA"

     misc_feature    <1325..1543
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1337..1339,1382..1384,1424..1426,1436..1438,
                     1490..1492,1511..1513,1517..1519)
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1676..2953
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2087..2089,2099..2101,2135..2146,2153..2155,
                     2177..2179,2186..2188,2198..2200,2210..2212)
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2291..2293,2297..2299,2507..2509,2921..2923)
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

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