LOCUS       XM_006519329            4994 bp    mRNA    linear   ROD 10-APR-2023
DEFINITION  PREDICTED: Mus musculus piwi-like RNA-mediated gene silencing 2
            (Piwil2), transcript variant X1, mRNA.
ACCESSION   XM_006519329
VERSION     XM_006519329.4
DBLINK      BioProject: PRJNA169
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_000080.7) annotated using gene prediction method: Gnomon,
            supported by mRNA and EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 21, 2020 this sequence version replaced XM_006519329.3.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_000001635.27-RS_2023_04
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           RefSeqFE; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 04/05/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /chromosome="14"
     gene            1..4994
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /note="piwi-like RNA-mediated gene silencing 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 2 mRNAs, 8 ESTs, 39 long SRA reads, 10 Proteins, and
                     99% coverage of the annotated genomic feature by RNAseq
                     alignments, including 2 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:57746"
                     /db_xref="MGI:MGI:1930036"
     CDS             276..3191
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /codon_start=1
                     /product="piwi-like protein 2 isoform X1"
                     /protein_id="XP_006519392.1"
                     /db_xref="GeneID:57746"
                     /db_xref="MGI:MGI:1930036"
                     /translation="

MDPVRPLFRGPTPVHPSQCVRMPGCWPQAPRPLEPAWGRAGPAGRGLVFRKPEDSSPPLQPVQKDSVGLVSMFRGMGLDTAFRPPSKREVPPLGRGVLGRGLSANMVRKDREEPRSSLPDPSVLAAGDSKLAEASVGWSRMLGRGSSEVSLLPLGRAASSIGRGMDKPPSAFGLTARDPPRLPQPPALSPTSLHSADPPPVLTMERKEKELLVKQGSKGTPQSLGLNLIKIQCHNEAVYQYHVTFSPSVECKSMRFGMLKDHQSVTGNVTAFDGSILYLPVKLQQVVELKSQRKTDDAEISIKIQLTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDSVLDVMHAIYQQNKEHFQDECSKLLVGSIVITRYNNRTYRIDDVDWNKTPKDSFVMSDGKEITFLEYYSKNYGITVKEDDQPLLIHRPSERQNNHGMLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLTQQINLSPKQHHGALECLLQRISQNETASNELTRWGLSLHKDVHKIEGRLLPMERINLRNTSFVTSEDLNWVKEVTRDASILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRISPPAWVELKDDRIETYIRTIQSLLGVEGKIQMVVCIIMGTRDDLYGAIKKLCCVQSPVPSQVINVRTIGQPTRLRSVAQKILLQMNCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKYYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFDNYHPKMVVFVVQKKISTNLYLAAPDHFVTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQILHHEPAIQLCGNLFFL"

     misc_feature    <279..875
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /note="large tegument protein UL36; Provisional; Region:
                     PHA03247"
                     /db_xref="CDD:223021"
     misc_feature    1284..1427
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    1428..1781
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /note="PAZ domain, Piwi_like subfamily. In multi-cellular
                     organisms, the Piwi protein appears to be essential for
                     the maintenance of germline stem cells. In the Drosophila
                     male germline, Piwi was shown to be involved in the
                     silencing of retrotransposons in the...; Region:
                     PAZ_piwi_like; cd02845"
                     /db_xref="CDD:239211"
     misc_feature    order(1560..1562,1599..1601,1623..1625,1635..1637,
                     1689..1691,1740..1742,1746..1748)
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239211"
     misc_feature    1803..3137
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; Region:
                     Piwi_piwi-like_Euk; cd04658"
                     /db_xref="CDD:240016"
     misc_feature    order(2310..2312,2322..2324,2358..2369,2376..2378,
                     2406..2408,2415..2417,2427..2429,2439..2441)
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     misc_feature    order(2502..2504,2508..2510,2712..2714,3111..3113)
                     /gene="Piwil2"
                     /gene_synonym="mili; Piwil1l"
                     /note="active site"
                     /db_xref="CDD:240016"
ORIGIN      

aaggcgtgccacctgtttgagagcaacctccacatagctagactgcgtgaaagggtgagccaatgaggtgagggactcttttgagtggtaggttgtttggccaatgagctgagcccttgggctagggatccggaaatcaggacctcgggcacgaggctcattccaagaggcagatgaggctgtgttctgcctagacagggggcttcaggaccctgacgaccctaggccagataaggaattggaacagaaccaacagtcgtttctacagcctctcaatggatcctgtcaggccgttgttcagggggcccaccccagtccacccatctcagtgtgtgcggatgccaggctgttggcctcaagctcctagacctttggaaccagcttggggtagggcaggacctgcaggcagaggccttgtgtttagaaaaccagaagactccagcccaccactccagccagtacaaaaggattctgtaggtttggtgtccatgttccgtggcatgggtcttgacacagcattccggcctccttcaaaacgagaagtgcctcctttaggcagaggagttctaggtcgaggcttgtctgctaacatggtccgcaaggacagagaagaaccccgttcctctttgcctgatccttcggtgctggcagctggggacagcaaactggcagaggcttctgttggttggagtagaatgctgggaagaggtagttctgaggtctctctgttaccactgggacgagcagccagtagtataggcagaggaatggacaaacctcccagtgcctttggcctcacagctcgggatcccccacggctgccacagcctccagctctgtctccaacttcactgcactctgccgatccccctccggtcctgactatggaacgaaaggaaaaagagcttttggtcaagcaaggatcaaaaggaactcctcagtctttgggactgaacctcatcaaaatccagtgtcataacgaagcagtttatcagtaccatgtgactttcagccccagtgtggaatgcaaaagcatgaggtttggcatgttgaaggaccaccagtctgtcactggaaacgtcactgcttttgatggctctattctttatcttcctgttaagcttcaacaagttgttgagttaaaaagtcagaggaaaactgacgatgccgagatcagtatcaagattcagctgacaaagatcctggagccgtgttctgacctgtgcatccccttctacaatgttgtcttccggcgggtaatgaaacttctggatatgaagcttgtggggagaaacttctatgaccctacaagtgccatggtactgcagcaacacagattgcagatctggcctggctatgcggctagtatccggaggacagacgggggtctcttcctgctcgctgatgtctctcataaggtcattcggaacgactctgtgctggatgtcatgcatgctatctaccagcagaacaaggagcacttccaggacgagtgcagcaagcttctggttggcagcattgtcatcacgcgctacaacaatcgtacctaccgaatcgatgatgtggactggaacaagacccctaaagacagctttgtcatgtcggacgggaaggaaatcacattcctggaatactacagcaaaaactatgggatcacagtcaaggaagatgaccagccgctgctgatccaccggcccagtgagagacagaataaccatggcatgttgctgaagggcgagatcctgctgctgcccgagctctccttcatgacggggatccctgagaagatgaagaaggacttcagggccatgaaggacttgactcagcagattaacctgagccccaagcagcaccacggtgctttggaatgcctgctgcagagaatttcacaaaacgagacagccagcaatgagctgacccgctgggggctcagtctgcataaagatgtccacaagattgaaggtcggcttctgccaatggagaggatcaacttaaggaacacttcatttgtcacatcggaggacctgaactgggttaaggaagtgaccagagatgcttccattctaactattcccatgcatttctgggcactcttttatccaaagagagcaatggaccaagccagagaactggttaacatgttggaaaagattgccgggcccattggcatgcgcataagccccccagcctgggttgagctgaaggatgaccgaatagagacctatatcaggaccattcagtccttactgggagttgaggggaagatacaaatggtcgtttgcatcatcatgggcacacgtgatgatctctatggagccatcaagaagctgtgctgcgtgcagtccccagtgccctcacaggtcatcaatgtccgaaccattggtcagcccaccaggcttcggagcgtggctcagaaaattttacttcagatgaactgtaaactgggtggtgagctctggggagtggatattccgctgaaacaactaatggtgattggaatggatgtgtaccatgaccccagcagaggcatgcgctctgtggtcggcttcgtggccagcataaatctcacactcaccaaatggtactcgagggtggtgttccagatgccacatcaggagattgtggacagcctgaagctctgcctggtgggttccttgaaaaagtattatgaggtgaaccattgtctcccagagaaaattgtggtgtaccgagatggagtgtctgatggccagctaaagacagttgccaactacgagatccctcagctgcagaagtgttttgaagcctttgataactaccaccccaagatggtggtgtttgtagttcagaagaaaatcagcaccaatctgtaccttgctgctcctgatcacttcgtaaccccctcccccgggactgtggttgatcataccataaccagctgtgagtgggtggatttctaccttcttgcccatcatgtgcgacagggctgtggcatacctacacactacatctgtgttctgaacactgcaaatctgagccctgatcacatgcagaggttgactttcaaactatgccacatgtactggaattggcctggtaccatccgagttccagctccttgcaagtatgcccacaagctagctttcctgtccggacagattttgcatcatgagccagccatccagctgtgtgggaacctgttcttcctgtaactgggaacttggacaccggctgcaaggagcaactggactcagctcagctcctccttacagaatcaacagaaatggcagtggaattttatgtttgcattttctctttctccatccttgtagaattagatttctgttcttcttttaaccctgatatcatagtagggtgttgtggtgcatgccttttatcccagcacttgggagactgaagcaggagtatctttagttcaaggccagcctagactacatggtgagttctaggctagccaagattacagagtgagaccctgtctcaaaaaacaaaaacaaaccactgtcccctcaaagcccacaacaaaaccaaagcctggggtcaaggaagcaagtttgtaggtagccgctggctgcccgctgccttcatggagtgtgtgccgtcagcgctgcttctcctcagccgagcgtggagcttcggacagggcagtgatgacatgttcttagcatgtcaaatccccctaccaaataagtcaagcaaggaaaaaatagcccccaaggcagcctgagcatcagttcctagaggttatgcctacagaaccatcctatttctggtggcagaagtgacatgaagtcattgcagacatcttaaaggagagttactgtgcagctgtctacatgtgtgaaagacatgtagaaaaaccagcgtagggtacagtcgggcgtatgtgcccacctgacccagggctgtgagtctgacttcccgagagtctggctagagctgcttttctggtccttttggttatttgcaaccatcatcagattgctttcctgcagcccgactgatacacgcatgtgcgcacgcacacacttttgttctttgtgactaatcttgaataaattcaattagaacacatggaaaggattcagcagacctaggaacatttggggtggagtgtagtttttctgcaaaagtctgtaaatgagattacgcaagagtctcttccagctgtgggctggtgttgcttggaaaatttcaaaatcccaaagtttcaggcttcccaaagttggcttggaaaaatgtgatagtctcacctgagtctagacatgtaggaaattttcctagggcctctgggcttcagtatttgggggaagcactggttttctgtgttatttttttccttctgtttcagaatcttcaagtttcttcaggcttcagtgggccatccctttactgggctctaaaagctaattttacttaaccttttcaaatgtgtatgtatctatttatgttttggtgtggtggatggatggtaggggactgagcagaaatagtcattttaaataacagtgtgctaggagagcctcagtgtgaagtcctgaggagcagcgggggctgtgggagtcagtgtcagccctcactcagacaggccaagcctgggctcgaagacaacattgtccagggaagcctttagtttgctatagcacccagaggttggcgaggaataagggttagggtcctcaaatacacccatggcttttggagtctatgaccaaggccaggctgagacctgaaatgtaaaagccatagaattaaacagaacagactgaaaaacgagtcttaagttccactttctggatttctctggaagtcttttgaatttgtctgtagaagttgctgttcctagcactccttttctctttaggtagaacagatacttgaccataatgccagaatgtactttctctgccttggtttttctatgccttgtttttcagtttcagggccaaacaattgggccctgtggtgtaaaataaaaacaatgtatgtgtata

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