LOCUS       NM_153403               7255 bp    mRNA    linear   ROD 05-AUG-2023
DEFINITION  Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript
            variant 1, mRNA.
ACCESSION   NM_153403 XM_994455 XM_994480
VERSION     NM_153403.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Muller M, Fah T, Schaefer M, Hermes V, Luitz J, Stalder P, Arora R,
            Ngondo RP and Ciaudo C.
  TITLE     AGO1 regulates pericentromeric regions in mouse embryonic stem
            cells
  JOURNAL   Life Sci Alliance 5 (6), e202101277 (2022)
   PUBMED   35236760
  REMARK    GeneRIF: AGO1 regulates pericentromeric regions in mouse embryonic
            stem cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Zhang Y, Tan YY, Chen PP, Xu H, Xie SJ, Xu SJ, Li B, Li JH, Liu S,
            Yang JH, Zhou H and Qu LH.
  TITLE     Genome-wide identification of microRNA targets reveals positive
            regulation of the Hippo pathway by miR-122 during liver development
  JOURNAL   Cell Death Dis 12 (12), 1161 (2021)
   PUBMED   34907157
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7255)
  AUTHORS   Bedogni F and Hevner RF.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7255)
  AUTHORS   Tang X, Miao Y, Luo Y, Sriram K, Qi Z, Lin FM, Gu Y, Lai CH, Hsu
            CY, Peterson KL, Van Keuren-Jensen K, Fueger PT, Yeo GW, Natarajan
            R, Zhong S and Chen ZB.
  TITLE     Suppression of Endothelial AGO1 Promotes Adipose Tissue Browning
            and Improves Metabolic Dysfunction
  JOURNAL   Circulation 142 (4), 365-379 (2020)
   PUBMED   32393053
  REMARK    GeneRIF: Suppression of Endothelial AGO1 Promotes Adipose Tissue
            Browning and Improves Metabolic Dysfunction.
            Erratum:[Circulation. 2021 Apr 20;143(16):e871. PMID: 33872082]
REFERENCE   5  (bases 1 to 7255)
  AUTHORS   Eswarappa SM, Potdar AA, Koch WJ, Fan Y, Vasu K, Lindner D, Willard
            B, Graham LM, DiCorleto PE and Fox PL.
  TITLE     Programmed translational readthrough generates antiangiogenic
            VEGF-Ax
  JOURNAL   Cell 157 (7), 1605-1618 (2014)
   PUBMED   24949972
REFERENCE   6  (bases 1 to 7255)
  AUTHORS   Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng
            EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC,
            Babbitt PC and Ferrin TE.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 7255)
  AUTHORS   Carmell MA, Xuan Z, Zhang MQ and Hannon GJ.
  TITLE     The Argonaute family: tentacles that reach into RNAi, developmental
            control, stem cell maintenance, and tumorigenesis
  JOURNAL   Genes Dev 16 (21), 2733-2742 (2002)
   PUBMED   12414724
  REMARK    Review article
REFERENCE   8  (bases 1 to 7255)
  AUTHORS   Beier H and Grimm M.
  TITLE     Misreading of termination codons in eukaryotes by natural nonsense
            suppressor tRNAs
  JOURNAL   Nucleic Acids Res 29 (23), 4767-4782 (2001)
   PUBMED   11726686
  REMARK    Review article
REFERENCE   9  (bases 1 to 7255)
  AUTHORS   Cikaluk DE, Tahbaz N, Hendricks LC, DiMattia GE, Hansen D, Pilgrim
            D and Hobman TC.
  TITLE     GERp95, a membrane-associated protein that belongs to a family of
            proteins involved in stem cell differentiation
  JOURNAL   Mol Biol Cell 10 (10), 3357-3372 (1999)
   PUBMED   10512872
  REMARK    GeneRIF: describes cloning rat sequence and used RNA interference
            to show that the GERp95 orthologue in C. elegans is important for
            maturation of germ-line stem cells in the gonad.
REFERENCE   10 (bases 1 to 7255)
  AUTHORS   Araki K, Imaizumi T, Sekimoto T, Yoshinobu K, Yoshimuta J, Akizuki
            M, Miura K, Araki M and Yamamura K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ130124.1, BC129914.1,
            AK080954.1, CV559803.1, AL606976.25 and AI451331.1.
            
            On Nov 19, 2015 this sequence version replaced NM_153403.2.
            
            Summary: This gene encodes a member of the argonaute family of
            proteins, which associate with small RNAs and have important roles
            in RNA interference (RNAi) and RNA silencing. This protein binds to
            microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses
            translation of mRNAs that are complementary to them. It is also
            involved in transcriptional gene silencing (TGS) of promoter
            regions that are complementary to bound short antigene RNAs
            (agRNAs), as well as in the degradation of miRNA-bound mRNA
            targets. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene. A recent study
            showed this gene to be an authentic stop codon readthrough target,
            and that its mRNA could give rise to an additional C-terminally
            extended isoform by use of an alternative in-frame translation
            termination codon. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes two isoforms, which result from the use of
            alternative in-frame translation termination codons. The shorter
            isoform (1) results from translation termination at the upstream
            UGA stop codon, while the longer isoform (1x) results from UGA stop
            codon readthrough to the downstream UAG termination codon. This
            RefSeq represents the shorter isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC129914.1, AB081471.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-162               CJ130124.1         2-163
            163-2736            BC129914.1         1-2574
            2737-3224           AK080954.1         2425-2912
            3225-3556           CV559803.1         331-662
            3557-7095           AL606976.25        152062-155600       c
            7096-7255           AI451331.1         2-161
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 60.76 cM"
     gene            1..7255
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="argonaute RISC catalytic subunit 1"
                     /db_xref="GeneID:236511"
                     /db_xref="MGI:MGI:2446630"
     exon            1..187
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     CDS             163..2736
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Piwi/Argonaute family protein meIF2C1; argonaute 1;
                     protein argonaute-1; mAgo1; eIF2C 1; eIF-2C 1; argonaute1;
                     eukaryotic translation initiation factor 2C, 1; argonaute
                     RISC catalytic component 1"
                     /codon_start=1
                     /product="protein argonaute-1 isoform 1"
                     /protein_id="NP_700452.2"
                     /db_xref="CCDS:CCDS18653.1"
                     /db_xref="GeneID:236511"
                     /db_xref="MGI:MGI:2446630"
                     /translation="

MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA"

     misc_feature    262..654
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:406799"
     misc_feature    682..834
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:400854"
     misc_feature    835..1197
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(991..993,1036..1038,1078..1080,1090..1092,
                     1144..1146,1165..1167,1171..1173)
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1087..1104
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG1.2);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    1330..2607
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    1726..1854
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG1.2);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    order(1741..1743,1753..1755,1789..1800,1807..1809,
                     1831..1833,1840..1842,1852..1854,1864..1866)
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1945..1947,1951..1953,2161..2163,2575..2577)
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="active site"
                     /db_xref="CDD:240015"
     misc_feature    2170..2187
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG1.2);
                     Region: Impairs access of bound RNA to the active site.
                     /evidence=ECO:0000250"
     misc_feature    2284..2298
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG1.2);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2413..2439
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG1.2);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2524..2601
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG1.2);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     exon            188..371
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            372..492
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            493..674
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            675..811
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            812..946
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1034
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1182
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1302
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1425
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1559
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1744
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1745..1904
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..1995
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2190
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2191..2325
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2326..2427
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2428..2627
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2628..7227
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    2848..2850
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="alternative translation termination codon, use of
                     which results in the longer, C-terminally extended isoform
                     1x"
ORIGIN      

ggcggcaacggaggctgcgggggcgacagcgcgagcggccgggcttcgggggggagccgagcccggcccgggagcccgagcagtgcaagtgcgaggtacctaggcccctcacgctggactcctcagtctcccggccgcctgtcctccgcacgggtatatgggatggaagcgggaccctcgggagcagcggcaggtgcctacctgcctcccctgcagcaggtgtttcaggcaccccgccggcctggcattggcactgtgggcaaaccaatcaagcttctggccaattactttgaggtggacattcctaagattgacgtttaccattacgaggtggacatcaagccggataagtgtcctcgcagagtcaaccgggaggtggtggaatacatggtccagcatttcaaaccgcagatctttggggatcgcaagcctgtgtatgatggaaagaagaatatttacacggtcacagcactgcccattggcaatgagagggttgactttgaggtgacaatccccggggaggggaaggatagaatttttaaggtctccatcaagtggctagccattgtgagctggcgcatgctgcatgaagccttggtcagtggccagatccctgtgcccctggagtctgtgcaagccctggatgtggccatgaggcacctggcatctatgaggtacacccctgtgggccgctccttcttctcaccgcctgagggctactaccacccgctggggggtgggcgcgaggtctggttcggctttcaccagtctgtgcgccctgccatgtggaagatgatgctcaacattgatgtctcagccactgccttctacaaagcacagccagtgattgagttcatgtgtgaggtcctggacatcaggaacatagacgaacaacccaagcccctcacggattcccagcgtgttcggtttaccaaggagataaaaggcctgaaggtggaagtgacccactgtggacagatgaagaggaaataccgcgtgtgtaatgttacccgccgccctgctagccatcagacgttccccttgcagctggagagtggacagactgtggagtgcactgtggcacagtatttcaagcagaaatataaccttcagctcaagtatcctcacctgccctgcctacaagttgggcaagaacagaagcatacctatttgcccctcgaggtctgtaacattgtggctgggcagcggtgcattaagaagctgactgacaaccagacttcaaccatgataaaggctacagctaggtcggccccagacagacaggaggagatcagccgcctgatgaagaatgctagctacaacctggatccctacatccaggaatttggaatcaaagtgaaggatgacatgacggaggtgacaggacgtgtgctgccggcgcccatcttgcagtatggcggccggaaccgggccattgctacgcccaaccaaggtgtctgggacatgcgcgggaaacagttctacaatgggattgagatcaaagtctgggccatcgcctgcttcgcaccccagaaacagtgtcgagaagaggtgctcaagaacttcacagaccagcttcggaagatttccaaggatgcagggatgcccatccagggtcagccatgcttctgcaaatacgcacagggggcagacagcgtggagcccatgttccggcatctcaagaacacctactcaggactgcagctcattatcgtcatcctgcccgggaagacgccagtgtatgctgaagtgaaacgtgtcggagatacactcttgggaatggcaacacagtgtgtgcaggtgaagaatgtggtcaagacgtcaccgcagactctgtccaacctctgcctcaagatcaatgtcaaactcggtggcattaacaacatcctagtcccacaccagcgctctgctgtttttcaacagccagtgattttcctgggagcagatgttacacatcccccagctggggatgggaagaaaccatctatcacagcagtggtaggcagcatggacgcacaccccagccgatactgtgccaccgtgcgtgtgcagcgcccacggcaggagatcattgaagacttgtcctatatggtgcgcgagctgcttatccagttctacaagtccacccgcttcaagcccacccgcatcatcttctaccgcgacggcgtccccgagggccagctgccccagattcttcactatgagctgcttgccattcgagatgcatgcatcaaactggaaaaagactaccagcctgggatcacatatattgtggtgcagaagcgccatcacacccgcctgttctgtgctgacaagaatgagcggattgggaagagtggtaacatcccagcagggaccactgtggacaccaacatcacccacccatttgagttcgacttctatctgtgcagccatgcaggcatccagggtaccagccgaccatcccattattatgtcctttgggatgacaaccgtttcacagcagatgaactccagatcttgacataccagctgtgccacacttacgtacgatgcacacgttctgtctctatcccagcacctgcctactatgctcgcttggtggctttccgggcacgataccacctggtggacaaggaacatgacagtggagaggggagccacatatcagggcaaagcaatgggcgagacccccaggccctggccaaagctgtgcaggttcaccaggatactctacgcaccatgtacttcgcttgaaggcagaacgctgttacctcactggatagaagaaagctttccaagccctgggagctgtaccacccaaatccagaggaagcaaggcagagggaggtggggtcggaaggagtataggaggccttgtttctatctacagagggggtataagggtgggaagaggaccagcaagacagactaccagccagaaatcactgatagcaacctcattgtcctcaacccctctccccatcttgtcatatccgacccccttggaccaaaaggggcatcactagtacccaccatacacacagttgtctcatgtgactcacagtgctaaagactcatgctcaacagcctgctagggtcagctctgcagccctgtggtcaaatgctggtatgtttgggtttgctattctagatgagaaagtgggtgggaagagggcaggaatttttaggagccttaatcagaaaaggtctagatttgtttaagaaaaaggaaaacagactcagatcaatattttaggatactagatgttttcatgggttcagaatccactttgtaggaagatttttaatgctaatggtttgggttgctcctccccagctgccatcccctcccatcattcctctccacattctctaccccatgcaccttttccctgacagacatccagccctcgtaacacttaaggcactatggcacttagctctgaggtgacatgaccctgtcttccttccgcctgctggtgggtaaccagtgccttcccgcaacggtaatgctgcagaactgcaaccttttgtacctttctctgtggggtggggtgtgggtgggaaaggagggagggagggagggcagaaatagcccaatgccagcgagcctccagccagaatgccagctaattttcatttgaaggaattgacttccataatcattgagcttttaaaaaaaggtcacaacctcaagatggttaaaatccattgacatttgcactttcagacgtgacaagtctccgagctgctgagatgactggcccttggcctttcccctttagcttcctcttttccctggccccaccaccaccctgccagatctagagttactgtcatagtatttctcattcttggcttttttacccctgatggtaaagtctcgtttcaatatttcttaactggggtgtcttacagcagaagatcgtaaggactaaaataagaaaacaaaaaatctaaaggttatttttataccataacttgagtctattgccaaaatctggaaattccctcccatacctgatgagtctgcattccagaaacatggagccgtaagagggaactgtggaactgacttgtgttctggcctagctaagtagggggtggttgtctccccaaggtggggctcagcctctgtccttcctctgtgcagacaaagccttgttctcgagacagcctccctccagggcgctgtcctgccctcttccctgtaagtgcatcttccccttttctggactggactgtcctttgatccctgactaaccgagactctagcacgtcctctggacaggctggggaattttgctgctcccatttgcttctgtttacacaaatgaaattttcctgtttctcactagggcatgtgagtgggtggcatgggcttttctttttctacccctctcttgaggttctggggttcagctagctgtcttgcaggactggaaaagaagtgggttctagctttgtctttgctgaccttgaagcaaggccccctctttccttgaccattcttttttcttcctgcattattgcttgggatgggaaatttgaacctccgtgtacaggttccccttctgaggagcctgagcttaggattgccctactctggcgatgaagccatgatatgttagctctagcccaggcttagaggcccgctggagaaaggaagatctgaaccctggcctatggagtcaaaacctttccccaagagcccatgtatagacccagatttgctatgcaaatagtccagattcagttatggctggctacattattcagtaacttcccaacaggtagcacaaatattccttatggaaaaagcccaggactgttcagtagttcctcctgtacttttctgctggctacagtatggagtgccccatgggcacaggcccagccggagaacagaacggagggctctgggaagaggcagctcactggagagcctacattccttacacaagtgcctaaacagagtgatgccaacactccatctgccctgtccatcgccttcatagacagtctacttcgtgttccatcaccctttggggagggagctctttgcagacagtgtgttgtcaacttgggtacatttgggggcttggattaagggcactactccaggagggtccaatcattcaccagcatttgcagatgcccatagggaacaggtggctgtcacaactcccagcaacaagattttgttttctcctttttctttgcctcactctctccagttgggttttaagctagaatgaaaagcagtttcggtcctttggtatttatgtgccattcatgaaataaaagcaagagaatgctgggcacgatggcccatgtctgtaattctgttatctcagcactaaggctgaagcagaagatttgcaagttcagggtccacctgggctactcagtgaattccaggccagtgtgcactatgtagcaagaccatgtctccataaaaaagaccaagaaaaatgacttgggtgacagatcttgctgtgattttaaataattctttgagacttaactactgaccatgtgggttcttctcatttaaaggcagtatgcacacattacaaacagtgttgccaagcctcagcttgtttggatttggtttaagtggtgcattgtaccgagctctgggaggattccagcctcatcttagacagctctttctcctcccccaactccctcccatgcctccaccccacccccactgttcggctttccctttcctcttctggccttccctggcttccttttccttctccctcttccctcagcttgcttgctggctggcctgcctgcctgtctgcctgtgtgataatgaattctctgcatggctgcaatgatcccactgttagttggcaaggtcaggcttagctcattgactacagaagaccaaaatcctgtttctcatgcccagtggtgttagccctgggttgcactgtcctcaagcttacactaggtcagagcagcaggcctgtcaaactgacaaaactagtagggtttctggcaggttcaacacctctccattctgtggaggggcttggagaagaggcagtgaaggagaccgctgctttgaattcagtccagtgtgaccgattggttgaggtgctattggcagcctgttggggtcggcctggagctactgttactgggaacttaaacttggactttgactaaagttgaacagagtatgaaactgttctagaaatctagttctcaactgcgacatagctcctctgtgactgagcagcaaggctttccaacaggttccttttaaccagaattctatgaatgatgccggggacacagttttcttagctggcccatagaaggtctacaaggctgctaagtgttgtagacctggcatctggcatcacataggaggatggaatttcttccctttcaggatagaagagtcacctcttacctagccaaaaggcagttttgggaattcctgtcaggaggaaaggtttggttttgttttttaatttactgggctttagatggtagttcattatgccttccttgggcagggatcatggcagggatacgcaaggcactctaatactcggccttctaagcaggctgggaaagggggattctctggttttctctttctccccacttactaggacttgccttctccctgagccacgagagaggctgggttggtgctctccccttactctgctcctactgacttagaacctctggctgctgtatgatagcccaggaaagggaatggaaagaatgggctcaaaacaataatgtaaggaaggtggtttccttccttaaaaagggaaatcagaaactctccaagaattgtgtaagtaaagacaatgtgtctgaatgcactgagtcctctggtgtcatagcaataaggaaaaatgaattgactttctgtgcatacagtccagcctgactggtgtatgatgttgcacttagcagccatgtggtgggcatgtgtgactactctgggtttcactttattttctaaactttttattcctctcaagtccagcatggatggggaaatgtccctggagccccgcagctgtgtacttgtttgcatttgtttcccattgagacttgtgtttgtgtcctgctttgagctgtaccctgtcccgtccattgtgaaattatcccagcagctgtaatgtacagttcccttctcaagcaagcaacagcagcacaatcttgtgttttataaaaacaacagtggcttttatttctaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa

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