LOCUS       NM_153402               7635 bp    mRNA    linear   ROD 26-SEP-2023
DEFINITION  Mus musculus argonaute RISC catalytic subunit 3 (Ago3), mRNA.
ACCESSION   NM_153402
VERSION     NM_153402.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Zhang Y, Tan YY, Chen PP, Xu H, Xie SJ, Xu SJ, Li B, Li JH, Liu S,
            Yang JH, Zhou H and Qu LH.
  TITLE     Genome-wide identification of microRNA targets reveals positive
            regulation of the Hippo pathway by miR-122 during liver development
  JOURNAL   Cell Death Dis 12 (12), 1161 (2021)
   PUBMED   34907157
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  AUTHORS   Adiliaghdam F, Basavappa M, Saunders TL, Harjanto D, Prior JT,
            Cronkite DA, Papavasiliou N and Jeffrey KL.
  TITLE     A Requirement for Argonaute 4 in Mammalian Antiviral Defense
  JOURNAL   Cell Rep 30 (6), 1690-1701 (2020)
   PUBMED   32049003
REFERENCE   3  (bases 1 to 7635)
  AUTHORS   Ingham NJ, Pearson SA, Vancollie VE, Rook V, Lewis MA, Chen J,
            Buniello A, Martelletti E, Preite L, Lam CC, Weiss FD, Powis Z,
            Suwannarat P, Lelliott CJ, Dawson SJ, White JK and Steel KP.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7635)
  AUTHORS   Hajarnis S, Yheskel M, Williams D, Brefort T, Glaudemans B, Debaix
            H, Baum M, Devuyst O and Patel V.
  TITLE     Suppression of microRNA Activity in Kidney Collecting Ducts Induces
            Partial Loss of Epithelial Phenotype and Renal Fibrosis
  JOURNAL   J Am Soc Nephrol 29 (2), 518-531 (2018)
   PUBMED   29021386
REFERENCE   5  (bases 1 to 7635)
  AUTHORS   Sun S, Ma J, Zhang Q, Wang Q, Zhou L, Bai F, Hu H, Chang P, Yu J
            and Gao B.
  TITLE     Argonaute proteins in cardiac tissue contribute to the heart injury
            during viral myocarditis
  JOURNAL   Cardiovasc Pathol 25 (2), 120-126 (2016)
   PUBMED   26764146
REFERENCE   6  (bases 1 to 7635)
  AUTHORS   McKee AE, Minet E, Stern C, Riahi S, Stiles CD and Silver PA.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 7635)
  AUTHORS   Liu J, Carmell MA, Rivas FV, Marsden CG, Thomson JM, Song JJ,
            Hammond SM, Joshua-Tor L and Hannon GJ.
  TITLE     Argonaute2 is the catalytic engine of mammalian RNAi
  JOURNAL   Science 305 (5689), 1437-1441 (2004)
   PUBMED   15284456
REFERENCE   8  (bases 1 to 7635)
  AUTHORS   Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K and Saigo K.
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells
            requires Dicer and eIF2C translation initiation factors
  JOURNAL   Curr Biol 13 (1), 41-46 (2003)
   PUBMED   12526743
REFERENCE   9  (bases 1 to 7635)
  AUTHORS   Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng
            EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC,
            Babbitt PC and Ferrin TE.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 7635)
  AUTHORS   Carmell MA, Xuan Z, Zhang MQ and Hannon GJ.
  TITLE     The Argonaute family: tentacles that reach into RNAi, developmental
            control, stem cell maintenance, and tumorigenesis
  JOURNAL   Genes Dev 16 (21), 2733-2742 (2002)
   PUBMED   12414724
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY235425.1, AK083652.1 and AL606976.25.
            
            On Jun 18, 2009 this sequence version replaced NM_153402.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK154436.1, AK035042.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                BY235425.1         15-48
            35-2536             AK083652.1         2-2503
            2537-3159           AK083652.1         2505-3127
            3160-7635           AL606976.25        57596-62071         c
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 60.39 cM"
     gene            1..7635
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="argonaute RISC catalytic subunit 3"
                     /db_xref="GeneID:214150"
                     /db_xref="MGI:MGI:2446634"
     exon            1..275
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    254..256
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="upstream in-frame stop codon"
     CDS             257..2839
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /EC_number="3.1.26.n2"
                     /note="argonaute 3; eukaryotic translation initiation
                     factor 2C, 3; argonaute RISC catalytic component 3;
                     Piwi/Argonaute family protein meIF2C3; mAgo3; eIF2C 3;
                     eIF-2C 3; argonaute3"
                     /codon_start=1
                     /product="protein argonaute-3"
                     /protein_id="NP_700451.2"
                     /db_xref="CCDS:CCDS18652.1"
                     /db_xref="GeneID:214150"
                     /db_xref="MGI:MGI:2446634"
                     /translation="

MEIGSAGPIGAQPLFIVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSVKFVSRVSWHLLHEALAGGTLPEPLELDKPVSTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNFFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA"

     misc_feature    257..259
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0000250|UniProtKB:Q9H9G7; propagated from
                     UniProtKB/Swiss-Prot (Q8CJF9.2); acetylation site"
     misc_feature    473..757
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    785..937
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    938..1300
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1094..1096,1139..1141,1181..1183,1193..1195,
                     1247..1249,1268..1270,1274..1276)
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1433..2710
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1844..1846,1856..1858,1892..1903,1910..1912,
                     1934..1936,1943..1945,1955..1957,1967..1969)
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    1844..1957
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJF9.2);
                     Region: Interaction with guide RNA.
                     /evidence=ECO:0000250|UniProtKB:Q9H9G7"
     misc_feature    order(2048..2050,2054..2056,2264..2266,2678..2680)
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="active site"
                     /db_xref="CDD:240015"
     misc_feature    2528..2671
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJF9.2);
                     Region: Interaction with guide RNA.
                     /evidence=ECO:0000250|UniProtKB:Q9H9G7"
     misc_feature    2729..2731
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9H9G7; propagated from
                     UniProtKB/Swiss-Prot (Q8CJF9.2); phosphorylation site"
     exon            276..447
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            448..568
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            569..777
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            778..914
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1049
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1137
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1285
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1405
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1528
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1662
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1847
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            1848..2007
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            2008..2098
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            2099..2293
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2428
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            2429..2530
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            2531..2730
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
     exon            2731..7635
                     /gene="Ago3"
                     /gene_synonym="C130014L07Rik; Eif2c3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gaccgggaaaagcgagcggctgcggcggcggggcggctggcgggtcgggaagctgcacgcgtcagcatctgtgggcgacgcaagttccggacctggttccagcgtcctcactcaggactgtcgccgctcttacctcgctgcaggctgtctgtctctcccgactgtgcctcttgtgcccggagctttccgagggcgtccgctcctcgcctctgtggtggcacccttctctcgtgaagcactccccccagctccatgaatggaaatcggctccgcaggacccattggggcccagcccctattcatcgtgcccagaagacctgggtatggcactatgggcaaacccattaaactgttggctaactgttttcaagttgaaatcccaaagattgatgtctacctctatgaagttgacatcaaaccagacaagtgtcctcgaagagtgaacagagaagtagttgattcaatggttcagcattttaaagtgactatatttggagaccgtagaccagtttatgatggaaaaagaagcctttatacagccaatccacttcctgtggcaaccactggggtagatttagatgttacattacctggggaaggtggaaaagatcgacctttcaaggtgtcagtcaaatttgtgtctcgagtcagctggcacctactacatgaagcactggctggagggactttgcctgagccactggaattagacaagcctgtcagcactaaccccgtccatgccgttgacgtggtgctccgacatctgccctctatgaaatatacgcctgtggggcgttcctttttctcagctccagaaggatatgaccaccctctgggagggggcagggaagtatggtttggattccatcagtctgttcggcctgccatgtggaaaatgatgctaaatattgatgtttctgccactgccttttacaaagcacaacctgtaattcagttcatgtgtgaagttcttgacattcacaacattgatgaacaacccagacccctgactgattctcatcgggtaaaattcactaaggagataaaaggtctgaaagttgaagtgactcattgtggaacaatgagacggaaataccgtgtttgtaatgtaacgagaaggcctgccagccaccaaacctttcctttacagctagaaaatggccaaactgtggagaggactgtggctcagtatttcagagaaaagtacacgctgcagctgaagtacccacaccttccctgcctgcaagtggggcaggagcagaagcatacatacctgccactagaagtctgtaatattgtggctgggcaaaggtgtatcaagaaactaacggacaatcagacttctacaatgataaaggcaacagcaagatctgcaccagatagacaagaggaaattagcagattggtaagaagtgcaaattatgaaacagatccatttgttcaggagtttcagtttaaagtgcgggatgaaatggcccatgtgacaggacgtgtacttccagcacctatgctccagtacggaggacggaaccggacagtagccacacccagccacggagtatgggacatgcgaggaaaacagttccacacaggagtcgaaatcaaaatgtgggctattgcttgctttgccacacagaggcagtgcagagaagaaatactaaaaggtttcacagaccagcttcggaagatttccaaggatgcagggatgcccatccagggtcagccatgcttctgcaaatacgcacagggggcagacagcgtggagcccatgttccggcatctcaagaacacctactcaggactgcagctcattatcgtcatcctgcccgggaagacaccagtgtatgcggaagtgaaacgtgtgggagatacacttttgggtatggccacacagtgtgtacaagtcaagaatgtaataaaaacatctcctcaaactctttcaaacttgtgcctaaagatcaatgttaaacttggaggaatcaataatattcttgtacctcatcaaagaccttctgtgttccagcaaccagtgatcttcctgggcgcagatgtcacacacccgcctgctggtgacggaaaaaagccctccattgctgctgttgtaggtagtatggatgcccatccaagcagatactgtgccacagtaagagttcagagaccccgacaggaaatcatccaggacttggcctccatggtccgggaactgctcatccaattttataaatcaacccgattcaaacctactcgtataatcttctatcgggatggtgtttcagaaggacagttcagacaggtattatattatgagctactggcaattcgagaagcctgtatcagtttggaaaaagactatcaacctggaataacctacattgttgttcagaagagacatcatacacggttgttctgtgctgataggacagaaagggttggaagaagcggcaacatcccagctggaaccacggtggataccgacattactcacccatatgaattcgacttttatctctgtagccatgctggaatacagggtaccagccgtccttcacactatcatgttttatgggatgacaacttctttactgcagatgaacttcagctgctcacttaccagctctgtcacacttacgtacgctgtacaagatctgtttctatccctgcaccagcatattacgctcacctggtggcattcagagccagatatcatcttgtggacaaggaacatgacagtgctgaaggaagccatgtttcaggacagagcaatgggcgagatccacaagctcttgccaaggctgtacagattcaccaagataccttacgcacaatgtacttcgcttaacaagtccaagtttattctccgtgacaaagagatgaaaagtgaatcaacatacaatgtatgtttccaatggagtcaatttgctggggaggcctccagccatacagaagccagtactatgtagaggtcaggtccttatgttgatccaaggaaaatcgtttacactgtcaaagaacgcagcaccattgtacactagggagccagccgctgcttttttgcagtctcctgtagaagatgcatcaccattgtttattctcacttcttacagtctaacccttttaatgcctttacctcaagttgcttagcagcacaactatctctgcaaaaaagtaaagaaaaattgaatggtggtttaaaaacacacaattacacaaataatgattgttcagttatatgtgcagaaaaagtaatgtgcatctattcttgtaaaggagtctgtattttcaataccctcacattacttcatgtgcatatgtatctagatctaggttactgatgtatatgtgtctgtgtgtatatcttatagttcatttccctggagaactctttccttgtatccctccttaggccgcactcgtttcctttactgtctcccttgccttcagcatctatactttggtccctatagctacaagtgacattcctatgttcacacatatggttcacttgaaggaaggtttgacaaattagacttctggttttctttatgtccctattcctagctcctttcactcacacaggagtcctcagctcctgacgccatgtttgtgagcgtctgctcccttttgctctggatttaccacatgtgcactgtgtttggaaacagtggcaacctggggattcttttttgtagtgagtttgttaaagctgctctaaatacgaaaccttgtcggacatctttctgatcagtgcaaatgttctaaagcctctgtttgtatgagttatacctggaaatccctccaaaactcgataggatggaaaaaggaaggaattagggttggtttggtttgttttttgctagtttactcactgcccttataccttaatgtgattttctcacatataaatatatacatacacacacacacattgaagtatagctccctttgcctttatgttcattagaatagtgctttaataattataaactatataggctatatcttttctagaactagaatcgtgtttaagaatggagacagttttttgctctcttttagtgcaataagcagttttacaggaaagttctgtctgtcatcctcaccgtggatagcaggaagaacagacatccgtagtttaaagggaacaaagtaatacagccttaatttcagaaggagagcttcataattttcagttttaaagcaattccttaaaataattttagccaaaggagtaaaaccgatcaaaggcgcctcagctgtgaggtcagaagcatagttgaacttaagtttttaaaaatgacagtgaacagcttaagatttaaatattctttcttacttattgaagaattgtgcttctttgactaacaggaattgagaaaatacaggatgatttttttctatctttttaccagtataactttctctttaaaaataagatggcaatgatgaaggaaattaggactttggtcatttaaaattagtaaaatttccgaaatacagtgaaaactttgcaaaaacttagttttctccacttctttaatcaataaccttaataacgttggagctgtcaaagctgagtgtctcagtaatggcaggttcagggaactttgcactttgtaagtcatgaacattgcatgtgagttttctctgttgtccccaccctggctctgaggacagacactacagatgtttgtggaatgcactgtcggcctttcagaagccccaccccgccatgaacaggaatgcagttatgtcttcatttgctctctttagtccacattgtagccaatttactacatttcaatataaaatctgagccatgtcctacaggcatgaattcttctgtccctttgccagaacataaagaaagtactgtattttatatttttattgcttgaagtatctaaaattcgattctcaatactgttttaaagctgtttactgtttacaaaggattttcatgtggtttatacatttttctccttccaaaatattcccatctcaaccctggcaaaagaaaactccttacaacatatttggggtgggttggtttgcttgcttgttttggagacaacgtctctctctattgcccatgctggcctccaacccctgggctccagcagtcttcttaacctctctggtagttggaaccattgggctccttccatcatgcctggctgtcggggagcatggccaaaagaatagagtggtgcaggctccatggtaaaagtctttgcctgggttgtgtttgctcagtagaagtaaacatgtttttcagcccctgcacattcacatgaggagctttagggagtcgcatgggccctgtccctttcctgacctttggtctcacacatgccagagtgaagcccttacttttaattcttatttaaaaggattttttaaggggaagaattttttttctaaaatataaggttttatcttttaaatggcttcttttttagaatgcatttatattattcagtcttgctagtttaagataattaacttaggatatgtatagtgtatttctgttttgcctactaataaattgttataccaaacttaatgccaacaaactttatgcttagaattttaagattaaaaggcctatagaaaatacttcttgaaatttatactatttttccatttctggttagaattcctaatctttttcttaagttcctttcagtttaaacatcatgaagccatagtaacatttctcctctaccgtactcttgctcctagctgatgaatgtcagcttgtggggtttttttgttttttatggtatggcttataaaactgactaaaatgtcaatctgaacaacttatagaatcgattctcttgttaagaagagttgagagtttaatgtatattcttgatggcaaatgtgtctgtagagtgtgcatgttacatgtctatattacagtatctaagcactggcattgataccatatattaataattctgtagctgctatatttattgaggcagagtctgacatttactgcactaaacaatgagacaagcaagatgccaggcagtcagacacttttggtttcttaggtaagtttaaggacataaaacatgaaggtaacgaacagcgggtacttgaaacagtgctggcttcagtttagtaacagaatacctttgctttagaaacgtggggtttttacattgacatccacatgagggtcttcagtcttctcagtcctgggcttgggcacatttctacataaatacggatgaccttttattttattaatagataattatcttttaacaattttccaggaaaaaatagtctggaaatacctctgcttatgacctattagtttatttttgttcctaaagagaatctggaagggactccagtaggaatgttctatgtgttttggaaggcatttgataaagattcatctctaccttctagcagagagactcctcaccttcaaaagacccaacaacttttctaagccaggctctctatggtgccagtagcctaataatgactttcatcgtgtcttgtagatcgcagggtggcaagtgtgtcttggggaattaaaaccagatgctgctgagtagaaatggctcgagatctcagtcacaggcggtgtgagcacttggagcccagccatagaagtgaagcccagagagctatccatccaaaacacgtggtgggcctttcagatgcttaattcttaggaaattcactttggacactggtagaatagtttgttccctttctgattaaaatttttagagtaatgttttgccaggaagtttaactgacatctctgcataattttgactgggtgtcagtaaactaagttatatgttcagttaagccaagttaaggtacacctataagcccagccctcaggagcatcagtaattcaaagccagcctcagcaacatgagaccatgcctcaaaacacagcaaacactttgtttacctcagccctgcaggtattctcctgctgcagttccgtgtagttcacttgtctcttagaagttgtgtgagtgcctcaaagaaagacagctcacttgtcaacattcgtgaagtgttggtccctttcatcctttttgagaattcatagatattttcgaagctttaataaaaatagctggagaacttaacttgagtgaggtcttcctgagtcttgttataatcctgacttgagtatcttcattttcctttccacttaacacagaatatttctgtgattctatgagtcaaaaactaagccaggaatacttatgtaaaggtcaatacagaggacaaggggacggctaagcaggtaaaggcacacccctgcaagcctgaaaacctgagctcaatccttggaacccaaacggtggaaagacagaaccagctcccataacttgccctctgaccttcatacacatgccatggcaccacccatcgggctcaagcacacgggcgtgcgcgggcacacacacactcacgcgtacttttaaatgtaatttttaagtctatagaaatcagaaaagcagctgggtttagtgcttgcctgtgatcctaaccagaagatctggagctcaggaccagcctgggcagtttgtctaggatacatagtgacactgtgtcccccgactttgccaaccccctgccccc

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