LOCUS       NM_153177               6527 bp    mRNA    linear   ROD 04-AUG-2023
DEFINITION  Mus musculus argonaute RISC catalytic subunit 4 (Ago4), mRNA.
ACCESSION   NM_153177
VERSION     NM_153177.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Zhang Y, Tan YY, Chen PP, Xu H, Xie SJ, Xu SJ, Li B, Li JH, Liu S,
            Yang JH, Zhou H and Qu LH.
  TITLE     Genome-wide identification of microRNA targets reveals positive
            regulation of the Hippo pathway by miR-122 during liver development
  JOURNAL   Cell Death Dis 12 (12), 1161 (2021)
   PUBMED   34907157
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Adiliaghdam F, Basavappa M, Saunders TL, Harjanto D, Prior JT,
            Cronkite DA, Papavasiliou N and Jeffrey KL.
  TITLE     A Requirement for Argonaute 4 in Mammalian Antiviral Defense
  JOURNAL   Cell Rep 30 (6), 1690-1701 (2020)
   PUBMED   32049003
REFERENCE   3  (bases 1 to 6527)
  AUTHORS   Hajarnis S, Yheskel M, Williams D, Brefort T, Glaudemans B, Debaix
            H, Baum M, Devuyst O and Patel V.
  TITLE     Suppression of microRNA Activity in Kidney Collecting Ducts Induces
            Partial Loss of Epithelial Phenotype and Renal Fibrosis
  JOURNAL   J Am Soc Nephrol 29 (2), 518-531 (2018)
   PUBMED   29021386
REFERENCE   4  (bases 1 to 6527)
  AUTHORS   Zheng Y, Guo X, He W, Shao Z, Zhang X, Yang J, Shen Y, Luo X and
            Cao J.
  TITLE     Effects of Echinococcus multilocularis miR-71 mimics on murine
            macrophage RAW264.7 cells
  JOURNAL   Int Immunopharmacol 34, 259-262 (2016)
   PUBMED   26995025
  REMARK    GeneRIF: up-regulated in murine macrophage RAW264.7 cells
            transfected by Echinococcus multilocularis miR-71
REFERENCE   5  (bases 1 to 6527)
  AUTHORS   Modzelewski AJ, Holmes RJ, Hilz S, Grimson A and Cohen PE.
  TITLE     AGO4 regulates entry into meiosis and influences silencing of sex
            chromosomes in the male mouse germline
  JOURNAL   Dev Cell 23 (2), 251-264 (2012)
   PUBMED   22863743
  REMARK    GeneRIF: show that Ago4(-/-) spermatogonia initiate meiosis early,
            resulting from premature induction of retinoic acid-response genes
REFERENCE   6  (bases 1 to 6527)
  AUTHORS   Lu J, Qian J, Chen F, Tang X, Li C and Cardoso WV.
  TITLE     Differential expression of components of the microRNA machinery
            during mouse organogenesis
  JOURNAL   Biochem Biophys Res Commun 334 (2), 319-323 (2005)
   PUBMED   16036130
REFERENCE   7  (bases 1 to 6527)
  AUTHORS   McKee AE, Minet E, Stern C, Riahi S, Stiles CD and Silver PA.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 6527)
  AUTHORS   Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga
            Y, Nagase T, Ohara O and Koga H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   9  (bases 1 to 6527)
  AUTHORS   Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K and Saigo K.
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells
            requires Dicer and eIF2C translation initiation factors
  JOURNAL   Curr Biol 13 (1), 41-46 (2003)
   PUBMED   12526743
REFERENCE   10 (bases 1 to 6527)
  AUTHORS   Carmell MA, Xuan Z, Zhang MQ and Hannon GJ.
  TITLE     The Argonaute family: tentacles that reach into RNAi, developmental
            control, stem cell maintenance, and tumorigenesis
  JOURNAL   Genes Dev 16 (21), 2733-2742 (2002)
   PUBMED   12414724
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB616446.1, BC096023.1, AK017827.1, AV306945.1 and AI481660.1.
            
            On Jan 9, 2009 this sequence version replaced NM_153177.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096023.1, AK030018.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-608               BB616446.1         1-608
            609-3912            BC096023.1         391-3694
            3913-6354           AK017827.1         1118-3559
            6355-6477           AV306945.1         133-255
            6478-6519           AK017827.1         3683-3724
            6520-6527           AI481660.1         1-8                 c
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 60.9 cM"
     gene            1..6527
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="argonaute RISC catalytic subunit 4"
                     /db_xref="GeneID:76850"
                     /db_xref="MGI:MGI:1924100"
     exon            1..274
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    58..60
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="upstream in-frame stop codon"
     CDS             256..2841
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="Piwi/Argonaute family protein meIF2C4; argonaute 4;
                     eukaryotic translation initiation factor 2C, 4; argonaute
                     RISC catalytic component 4"
                     /codon_start=1
                     /product="protein argonaute-4"
                     /protein_id="NP_694817.2"
                     /db_xref="CCDS:CCDS18654.1"
                     /db_xref="GeneID:76850"
                     /db_xref="MGI:MGI:1924100"
                     /translation="

MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRIDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKHPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPSQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKMNAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEITQELLYSQEVVQDLTSMARELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKMERVGKSGNVPAGTTVDSTVTHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA"

     misc_feature    331..720
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    751..903
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    904..1266
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1060..1062,1105..1107,1147..1149,1159..1161,
                     1213..1215,1234..1236,1240..1242)
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1405..2712
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1816..1818,1828..1830,1864..1875,1882..1884,
                     1906..1908,1915..1917,1927..1929,1939..1941)
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2020..2022,2026..2028,2266..2268,2680..2682)
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="active site"
                     /db_xref="CDD:240015"
     misc_feature    2728..2793
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJF8.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     exon            275..440
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            441..561
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            562..743
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            744..880
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1015
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1103
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1251
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1371
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1500
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1634
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1819
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..1979
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2295
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2296..2430
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2431..2532
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2732
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2733..6523
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6498..6503
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
     polyA_site      6523
                     /gene="Ago4"
                     /gene_synonym="5730550L01Rik; Eif2c4"
ORIGIN      

gctcccgttccagctggcggcggcggcggcagcggccgggattgtttttgttgtcgctgaggccggaagagccgtgacagagtcgggtccctgtccccggactgggcgccgccgccgccccctgcccgcccccggcccgcgtcttcgcggcgccgcttcagcgccaatattccagagagcaagcgttacgcggctccggcggggcccggagcgggaggcgctggggaccggagcgaggcggcccccgtcgccgccatggaggcgctgggacccggacctccagccagcctcttccagccacctcggcgtcctggccttggaaccgttgggaaaccaattcgactgttagccaatcattttcaggttcagatccctaaaatagatgtgtatcactatgatgtggatattaaaccagaaaaacggcctcgtagagtcaacagggaggtggtagacactatggtgcggcacttcaagatgcagatatttggagatcggcagcctggctacgatggcaaaagaaacatgtacacggcacacccactgccaatcggacgggataggattgatatggaggtgaccctcccaggtgagggtaaagaccaaactttcaaagtgtctgtgcagtgggtgtcagttgtgagccttcagctgcttttagaagctttagcaggacacctgaatgaagtcccagatgactcagtacaagcacttgatgtgattacaaggcatctcccctctatgaggtacactccagtaggccggtcttttttctcacctcctgaaggttattaccaccctctgggagggggcagagaggtttggtttggctttcatcagtctgtgagacctgccatgtggaatatgatgctcaatattgatgtatctgcaactgctttctaccgggctcaacctatcattgagttcatgtgtgaggttttagacattcagaacatcaatgagcagacaaaacctctaacagactcccagcgtgtcaagtttaccaaagaaattaggggtcttaaggttgaggtgactcactgtggacagatgaaacgaaaatatcgagtttgtaatgtgacaagacggccagccagtcatcaaaccttccctttgcagctagagaatggccaagctatggaatgtacagtggctcagtattttaagcagaagtatagtctgcagctgaaacacccccaccttccctgccttcaagtggggcaagagcaaaagcatacctacctaccacttgaggtctgtaatatagtggcaggacagagatgtataaagaagctcacagacaatcagacatccaccatgatcaaagccacggcaagatctgctcctgacagacaggaggaaatcagtagactggtgaagagcaacagcatggtgggtggacctgacccatacctgaaggaatttgggattgttgtgcacaacgagatgacagagctcacaggcagggtgcttccggctccaatgctgcagtatggaggccggaataaaacagtagccacacccagccagggtgtctgggacatgcgaggaaaacagttttatgctggcattgaaattaaagtatgggcagtggcttgttttgcgcctcagaaacaatgtagggaagatttgctaaagagtttcactgaccaacttcgtaaaatctccaaggatgcagggatgcccatccagggtcagccatgtttctgcaagtatgctcaaggtgcagacagcgtggagcccatgtttaaacatctgaaaatgacatatgtgggcctgcagctaatagtggtgattttgcctgggaagacaccagtatatgcggaggtgaagcgtgttggagatacccttctaggtatggccacacagtgtgtccaggtcaaaaacgttgtgaagacctcgccccagaccctttccaacctttgcctgaagatgaacgcaaagctgggagggattaacaatgtgcttgtgcctcaccaaaggccctcagtgttccagcagcctgtcatctttctgggagcagatgtcactcatccgcctgctggggacgggaagaagccttctattgcagccgtggtgggcagcatggacggccatcccagccggtactgtgccacagtccgggtacagacatctcgacaggagatcacccaggagctcctctacagccaggaggtcgtgcaggacctgacgagcatggctcgggagctgctgattcaattctataagtccacgcgcttcaagcccacacgcatcatctactaccgcggaggggtatccgagggacagatgaagcaggtagcttggccggagctaatagcaattcgaaaggcatgtataagcttggaggaagactatcggccgggaataacttacattgtggtgcaaaagaggcaccacacacgactcttctgtgcagataaaatggaaagggtggggaaaagtggcaatgtcccagcaggcaccacagtggatagcaccgtcacacacccgtctgagtttgacttttacctctgtagtcatgcaggaattcagggaaccagccgtccttcacattaccaggtcttgtgggatgacaactgcttcactgcagatgaactccagctactgacttaccagctctgtcacacctacgtgaggtgcacacgctccgtctccattcctgcccctgcatattatgcccggcttgtggcattccgggcaaggtatcatttggtggataaagatcatgacagtgcggaaggcagtcacgtgtcaggacagagcaacggccgggatcctcaggccttggctaaggctgtgcagatccaccatgatacccagcacactatgtattttgcctgagagtcttggaaaagaactcaacaaatttggcatctcacgcatcctcaaaatgtttccgacgcctactgcctctctcaggtggtccctaccagcagctcagaatggttgcactgagtctactctgcagccctgtctgatacatcaacaaaattgagccagttgggtttttttgttaaagaaatagatactcatagattgtgttttctgatgcactgggcagaatttttacctcaacgtgcaaaggctgatcagccttgactttctaaaggactttccaagaagggtttctatggaatcttttgctagctgtggtattcaccgcatctagtcttataagaggagtatttccttttttctgtgtttattgaatggtgtgcataagtgggagagagaaaccaaacggcctgtatcatttccatgtaactttttaattgggtgggtccatagacctcttacttctgactttgccactgtcaatgcctttaaggagcatcagcttttgaaagttttacagaactctgttaccttcactgttctactgatctcttcgggcctgcactgtggggtacaggctgcccagcaattgcactatatttggctgcagattcgaacaggcagttcccttacttgactggcttttgtgagtctgtgacataagcagatgtgatgtgtgtcacagggacagatacactgccgtggtaaaaagtacccatgttttccatatttggaaaaagagaattagcttttagtatttttccacatcttcaaacatgcccattttatgctgctgtgtggtatgctagatctaaacgatctttaaacttctctcatagaaagttaactttggcaataagcatttgtaaggcccttgccctttctcctgacgtcgttttctagatgagatttcagtatgattttatcaactatttcttacatatcataactgggatattctgaaaccagtttataaagacacagatttcaatgtccttataaataattgtttttaaaacaaaacaaaacaaaacttattttatagtcagtgtcctgtcactttaaaaaaaaaaaaaagaagctggaaccaggatgctttgtgatcggtcaggctttgccgatgcataaggcacattgagcagtactgcacttgaaaagaaagtaccagccagtgaaaacctggtttccctgggaatcaggggtgcacacacggctgcctacaagacagcctgaaatgtgcttggtggctctgcctttctctttttcaactaaaactctgtagaagttgttgttgttgttgttgtttttctttttctgtaacagattctagattccctcacaggaagacaggattccgtttcttgtccaaacaaagggaattgatgtaaagcaagaacagttagaacatagggaaaatgtattctgtatttagtgtagataataccttgtaaaggacaagtgttacatagtcaaggtagttgcacccacggtttccagcccatgttttactctaacctggaaggtcaaggagtaaaaggtaagaggctctctgtagagccacactcctcatggctgaggagggtgctggggaaggaagagacaggcacaatatgctaaaagcaattttgcagtctcggtgtagaaaccattggtgggaaacactggatcaaatgggaaagggtgtttgcttgaactcattaattggtccaaggttcaaatctgttaaatgggccctctcagaatcccagcaatagaaagcactgtttcccttcagtggcttcagcaagtgtgtagatacattcatgtttacatttctgtgtgcagtcgctcagcagtgccactggtgtttgctgagcctggtctccgagagagtttataatgtccgtctagtcctatagcacatgcggatgggtgcagacatctttaatctctgcagacagtgggaaaagcaagtcctgtgactgaagcacaacacaggcgtgaagcgacagcagacgtccgttccctgagatcttcctttttttctgcaaatacctccctgggataatacaaatccagacatgttgatgaatggctgactgccgcttttattcacacctgttcagggaaagcacagaaaatgctctgtagcacagacattctggacccaagaggagggggaacaaaggaagcctttttaggaaaaggaaaacttgcctggagtctgtgatgtgaagaactctcggtggctggcagctgacagaaggcaccgtacctgcacaggagcgcctcgggcttgcttgttgacaatgcacagaagagactctagccatcttccgacaggatggaacagtgagcgagctgtgtgcccggaagccccatccacggtcctgcaactgagttgtaggtttgcccatgagcgatatgcctgaaacaccactgccggtcctagtatgctggggtgaatccgtgtggagctggaggaaaagccaggagaccaacttttgtagaaaaaacggtatcaataagtgtgtgaagccgttgttctgggcaagtggaaagaaatatggaagagttaggattacttgggggaggggaacttgctgaggggtgaacagtccagaggtgatcacttgagaatcagaactgcagtaataccaccgtaagcaaatctgaatgcatagttttaacttaactggggttgacaattcaaataggtacctccccccacccccccaaacaaaaacctgactgagttgttatgcatgtgtcaatcactgggaggtgtgcattctctagtagtgttaggctgtttggttcttttaaatgcagcctatttaggtactgtcatgtagtgtggaagattttggtgcggcaactctaaagaatgacatttttggggctagggagcataggacattaagaacgggaaagacccaagtgttgaaccaattgtgtttttttgttgttagttgttgaatcctgagttgatgcagtcagcattctgggcaggcgaaccacctttgttcttactgttaagttctttgtgctgcttccaaagatgatttacaagcagggatgttctaaatgggtctgtaccttagggtccggggtccagcccatatcctaggtcaaagcctacccacctggctcagctacctactgctttaaccaggctcatcctctctactccgctgtggttgccctccacggatgtgctctgagattctcttgctcagtggcaaggtggctgctctgctttcatttttaaaaaagcggaagttaaggtcatcatactaatactgcatcaactccaggaattaaaggaaagatctttattgctcaaccattttcttattggttcctactgacatattcatctcctttctgtgtctactggctgaaatgtttgtgtttgttcatctgactaacggtatggtttctgtatggttagacttgtaatgttttaaaatgtattttattactttggactggatgttcctctccagcgatacgaggtactttctaagctattaaggaagggtttgaatccctatgtgagataagataatggttgtttaaattttgcagaggtttttgttattgttactggttttgattttgggtttttttttgacctgtggggatattttgtgtttatgtgtccaaaaagtggggaggggaataaattgacattcttgtgctaaaaaaaa

//