LOCUS       NM_001426554            2353 bp    mRNA    linear   ROD 15-JUN-2024
DEFINITION  Mus musculus Iroquois homeobox 4 (Irx4), transcript variant 2,
            mRNA.
ACCESSION   NM_001426554 XM_006517294
VERSION     NM_001426554.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Narwidina,A., Miyazaki,A., Iwata,K., Kurogoushi,R., Sugimoto,A.,
            Kudo,Y., Kawarabayashi,K., Yamakawa,Y., Akazawa,Y., Kitamura,T.,
            Nakagawa,H., Yamaguchi-Ueda,K., Hasegawa,T., Yoshizaki,K.,
            Fukumoto,S., Yamamoto,A., Ishimaru,N., Iwasaki,T. and Iwamoto,T.
  TITLE     Iroquois homeobox 3 regulates odontoblast proliferation and
            differentiation mediated by Wnt5a expression
  JOURNAL   Biochem Biophys Res Commun 650, 47-54 (2023)
   PUBMED   36773339
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Cattaneo,P., Hayes,M.G.B., Baumgarten,N., Hecker,D., Peruzzo,S.,
            Aslan,G.S., Kunderfranco,P., Larcher,V., Zhang,L., Contu,R.,
            Fonseca,G., Spinozzi,S., Chen,J., Condorelli,G., Dimmeler,S.,
            Schulz,M.H., Heinz,S., Guimaraes-Camboa,N. and Evans,S.M.
  TITLE     DOT1L regulates chamber-specific transcriptional networks during
            cardiogenesis and mediates postnatal cell cycle withdrawal
  JOURNAL   Nat Commun 13 (1), 7444 (2022)
   PUBMED   36460641
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2353)
  AUTHORS   de Soysa,T.Y., Ranade,S.S., Okawa,S., Ravichandran,S., Huang,Y.,
            Salunga,H.T., Schricker,A., Del Sol,A., Gifford,C.A. and
            Srivastava,D.
  TITLE     Single-cell analysis of cardiogenesis reveals basis for organ-level
            developmental defects
  JOURNAL   Nature 572 (7767), 120-124 (2019)
   PUBMED   31341279
REFERENCE   4  (bases 1 to 2353)
  AUTHORS   Wang,Y., Lu,P., Wu,B., Morrow,B.E. and Zhou,B.
  TITLE     NOTCH maintains developmental cardiac gene network through WNT5A
  JOURNAL   J Mol Cell Cardiol 125, 98-105 (2018)
   PUBMED   30347193
REFERENCE   5  (bases 1 to 2353)
  AUTHORS   Kiyama,T., Chen,C.K., Wang,S.W., Pan,P., Ju,Z., Wang,J., Takada,S.,
            Klein,W.H. and Mao,C.A.
  TITLE     Essential roles of mitochondrial biogenesis regulator Nrf1 in
            retinal development and homeostasis
  JOURNAL   Mol Neurodegener 13 (1), 56 (2018)
   PUBMED   30333037
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2353)
  AUTHORS   Bruneau,B.G., Bao,Z.Z., Fatkin,D., Xavier-Neto,J.,
            Georgakopoulos,D., Maguire,C.T., Berul,C.I., Kass,D.A., Kuroski-de
            Bold,M.L., de Bold,A.J., Conner,D.A., Rosenthal,N., Cepko,C.L.,
            Seidman,C.E. and Seidman,J.G.
  TITLE     Cardiomyopathy in Irx4-deficient mice is preceded by abnormal
            ventricular gene expression
  JOURNAL   Mol Cell Biol 21 (5), 1730-1736 (2001)
   PUBMED   11238910
REFERENCE   7  (bases 1 to 2353)
  AUTHORS   Peters,T., Dildrop,R., Ausmeier,K. and Ruther,U.
  TITLE     Organization of mouse Iroquois homeobox genes in two clusters
            suggests a conserved regulation and function in vertebrate
            development
  JOURNAL   Genome Res 10 (10), 1453-1462 (2000)
   PUBMED   11042145
REFERENCE   8  (bases 1 to 2353)
  AUTHORS   Christoffels,V.M., Keijser,A.G., Houweling,A.C., Clout,D.E. and
            Moorman,A.F.
  TITLE     Patterning the embryonic heart: identification of five mouse
            Iroquois homeobox genes in the developing heart
  JOURNAL   Dev Biol 224 (2), 263-274 (2000)
   PUBMED   10926765
REFERENCE   9  (bases 1 to 2353)
  AUTHORS   Christoffels,V.M., Habets,P.E., Franco,D., Campione,M., de Jong,F.,
            Lamers,W.H., Bao,Z.Z., Palmer,S., Biben,C., Harvey,R.P. and
            Moorman,A.F.
  TITLE     Chamber formation and morphogenesis in the developing mammalian
            heart
  JOURNAL   Dev Biol 223 (2), 266-278 (2000)
   PUBMED   10882515
  REMARK    Erratum:[Dev Biol 2000 Sep 1;225(1):266]
REFERENCE   10 (bases 1 to 2353)
  AUTHORS   Bruneau,B.G., Bao,Z.Z., Tanaka,M., Schott,J.J., Izumo,S.,
            Cepko,C.L., Seidman,J.G. and Seidman,C.E.
  TITLE     Cardiac expression of the ventricle-specific homeobox gene Irx4 is
            modulated by Nkx2-5 and dHand
  JOURNAL   Dev Biol 217 (2), 266-277 (2000)
   PUBMED   10625552
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154266.2.
            
            On Dec 19, 2023 this sequence version replaced XM_006517294.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.294265.1,
                                           SRR17784643.904547.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849388, SAMN00849390
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-130               AC154266.2         56424-56553
            131-262             AC154266.2         60748-60879
            263-514             AC154266.2         61358-61609
            515-627             AC154266.2         62496-62608
            628-956             AC154266.2         63404-63732
            957-2353            AC154266.2         64126-65522
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 39.64 cM"
     gene            1..2353
                     /gene="Irx4"
                     /note="Iroquois homeobox 4"
                     /db_xref="GeneID:50916"
                     /db_xref="MGI:MGI:1355275"
     exon            1..130
                     /gene="Irx4"
                     /inference="alignment:Splign:2.1.0"
     exon            131..262
                     /gene="Irx4"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    203..205
                     /gene="Irx4"
                     /note="upstream in-frame stop codon"
     CDS             218..1765
                     /gene="Irx4"
                     /note="homeodomain protein IRXA3; iroquois homeobox
                     protein 4; Iroquois related homeobox 4"
                     /codon_start=1
                     /product="iroquois-class homeodomain protein IRX-4"
                     /protein_id="NP_001413483.1"
                     /db_xref="GeneID:50916"
                     /db_xref="MGI:MGI:1355275"
                     /translation="

MSYPQFGYPYSSAPQFLMTTNSLSTCCESGGRTLADSGPAASAQAPVYCPVYESRLLATARHELNSAAALGVYGSPYGSSQGYGNYVTYGSEASAFYSLNSFESKDGTGSSHAGLPPTAAAAYYPYEPALSQYPYDRYGTVDSGTRRKNATRETTSTLKAWLQEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWPPRNKCADEKRPYGEGEEEEAGEEESREEPLKSAKSEGHAGKDDKELELSDLEDFDPLDAETSECELKTPFQSLDSGPERIPASSDGPGTGKEASTTLRMPLGTAGGAVMDGDLERARNCLRSTVVVPDSGAEGGPPACEAKLTFAQAGAPPNLETKPRIWSLAHTATAAAATALSQTEFPSCMLKRQGPTGVSATTPASSPAVTAPSGALDRHQDSPVTSLRNWVDGVFHDPILRHSTLNQAWATAKGALLDPGPLGRNLGAGTNVLTTPLACSFPPTVPQDVPPAGASRELLATPKAGGKPFCT"

     misc_feature    830..991
                     /gene="Irx4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QY61.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1049..1138
                     /gene="Irx4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QY61.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1409..1492
                     /gene="Irx4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QY61.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     exon            263..514
                     /gene="Irx4"
                     /inference="alignment:Splign:2.1.0"
     exon            515..627
                     /gene="Irx4"
                     /inference="alignment:Splign:2.1.0"
     exon            628..956
                     /gene="Irx4"
                     /inference="alignment:Splign:2.1.0"
     exon            957..2353
                     /gene="Irx4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2308..2313
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Irx4"
                     /note="hexamer: AATATA"
     regulatory      2322..2327
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Irx4"
                     /note="hexamer: AATAAA"
     polyA_site      2327
                     /gene="Irx4"
     polyA_site      2353
                     /gene="Irx4"
                     /note="major polyA site"
ORIGIN      

gctctgctctccaggtgctccgcctggaaatgtctccattagttgtcgcttgctccccgccggagcgcgcgcgcccagcggccgcgcagagccctagcgggcagccaggagtggcccggcgccactgcagagcctaggatcctacaactccggagcagtgcccacgctgtgcacaggaccccaacggcccctcagaccttcttaggccgcacccgccatgtcctacccgcagtttggatacccctattcctctgctcctcagttcctgatgaccaccaactccctgagcacgtgctgcgagtcgggcggccgcacactggctgattccgggcctgcagcctcggcacaggcgcccgtctactgccctgtctacgagagccggctgctggccactgcacgccacgaactcaattctgccgccgcgctcggcgtctacgggagcccctatggcagctcgcagggctatggcaactacgtgacctatggctctgaggcatctgctttctactctctgaacagcttcgagtccaaggatggcacaggatcttcacacgccggcctaccacccactgcagctgcagcctactacccgtatgagccagctctgagccagtacccatatgacaggtacgggaccgtggacagcggcacgcggcgcaagaatgccacccgggagaccaccagcacactgaaggcctggctgcaggagcaccggaagaacccgtaccccaccaaaggagagaagatcatgctggccatcatcaccaagatgaccctcacacaggtttccacctggtttgccaacgcacgtcggcgcctcaagaaggagaacaagatgacctggccacctagaaataaatgtgcagatgagaagcgcccctatggggagggggaggaggaggaggctggagaggaggagtcacgggaggagcctctcaagagtgccaagagcgaaggtcatgctggcaaagacgacaaggagctagaactcagtgacttggaagacttcgaccctctagatgcagagacctcagaatgtgagctgaagacgcccttccagtccctggatagtggaccagagaggattcctgcttcatctgatggtcccggcacaggcaaggaggcctccaccacactccggatgcccctgggcaccgctggtggagctgttatggatggggatctggagagggcaaggaactgcctccggagcacagtggtcgttcctgactctggcgccgagggcggcccacctgcttgcgaagccaaactgacctttgctcaggcgggagcgccgccgaacctggagaccaagccccgcatctggtccttggcacacacagccaccgctgctgcagccacagctctgagccagactgagtttccctcctgcatgctgaaacgccaaggtccaacgggtgtatctgcgacgacgccagcgtcctcacctgcagtcacggccccttctggggctctggataggcaccaggactccccagtaactagcctcagaaactgggtggatggggtatttcacgatcccatccttagacacagcactttgaaccaggcctgggccaccgctaagggcgcacttctggatcccggccctctaggacgcaatctgggggcgggtactaacgtactgacgacgccccttgcttgctccttcccgcccacggtgccccaagacgtcccacctgcaggggcctcccgggagctgctggctacgcccaaggccggtggcaaacccttctgcacttaacacccacctgcacagtggagaagggaactggcgctcaggctgcaggcgctgactctgaactgctttaaatgggtttccaaaagaagggggaatttccaagccaagattgccaacccacttgagacagaggatttgaactcaacctccagggctatggaggaactcctcgcgttctatcaccaggttttacctgatgttgcgtccacctgagccatcccttaactccaggaagatgagaagaccggagagcacagccgatgggaggccccctgaagcataaagtttacgtccaaagtttacatggagaaggcatttgggtactgaggcttgttttgtgtcgctgtctccttctgggtttgaggcatgccggtgttcgtgcgaagagtcttcagcccgtttttcttcggacttcatcctcagcacgtagagctcaccgccaacacttccaccgagtttacactgttgcacactcttgactcgacaaaattatgttttctaaaatgtatattcacaccagtaattgcctgtttcagagggaggctaatataacaaaactaataaattttcgtgtttgcattgcggaaggaa

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