LOCUS       NM_001425672            3658 bp    mRNA    linear   ROD 22-NOV-2023
DEFINITION  Mus musculus claudin 12 (Cldn12), transcript variant 5, mRNA.
ACCESSION   NM_001425672 XM_030254750
VERSION     NM_001425672.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Chen JT, Hu X, Otto IUC, Schurger C, von Bieberstein BR, Doppler K,
            Krug SM, Hankir MK, Blasig R, Sommer C, Brack A, Blasig IE and
            Rittner HL.
  TITLE     Myelin barrier breakdown, mechanical hypersensitivity, and
            painfulness in polyneuropathy with claudin-12 deficiency
  JOURNAL   Neurobiol Dis 185, 106246 (2023)
   PUBMED   37527762
  REMARK    GeneRIF: Myelin barrier breakdown, mechanical hypersensitivity, and
            painfulness in polyneuropathy with claudin-12 deficiency.
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Xing W, Pourteymoor S, Chen Y and Mohan S.
  TITLE     Targeted Deletion of the Claudin12 Gene in Mice Increases Articular
            Cartilage and Inhibits Chondrocyte Differentiation
  JOURNAL   Front Endocrinol (Lausanne) 13, 931318 (2022)
   PUBMED   35937800
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3658)
  AUTHORS   Cao H, Ni C, Han L, Wang R, Blasig R, Haseloff R, Qin Y, Lan J, Lou
            X, Ma P, Yao X, Wang L, Wang F, Zhu L, Lei N, Blasig IE and Qin Z.
  TITLE     Claudin-12 Deficiency Inhibits Tumor Growth by Impairing
            Transendothelial Migration of Myeloid-Derived Suppressor Cells
  JOURNAL   Cancer Res 82 (13), 2472-2484 (2022)
   PUBMED   35580275
REFERENCE   4  (bases 1 to 3658)
  AUTHORS   Beggs MR, Young K, Pan W, O'Neill DD, Saurette M, Plain A, Rievaj
            J, Doschak MR, Cordat E, Dimke H and Alexander RT.
  TITLE     Claudin-2 and claudin-12 form independent, complementary pores
            required to maintain calcium homeostasis
  JOURNAL   Proc Natl Acad Sci U S A 118 (48) (2021)
   PUBMED   34810264
  REMARK    GeneRIF: Claudin-2 and claudin-12 form independent, complementary
            pores required to maintain calcium homeostasis.
REFERENCE   5  (bases 1 to 3658)
  AUTHORS   Higashi AY, Higashi T, Furuse K, Ozeki K, Furuse M and Chiba H.
  TITLE     Claudin-9 constitutes tight junctions of folliculo-stellate cells
            in the anterior pituitary gland
  JOURNAL   Sci Rep 11 (1), 21642 (2021)
   PUBMED   34737342
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3658)
  AUTHORS   Hashizume A, Ueno T, Furuse M, Tsukita S, Nakanishi Y and Hieda Y.
  TITLE     Expression patterns of claudin family of tight junction membrane
            proteins in developing mouse submandibular gland
  JOURNAL   Dev Dyn 231 (2), 425-431 (2004)
   PUBMED   15366020
REFERENCE   7  (bases 1 to 3658)
  AUTHORS   Takasato M, Osafune K, Matsumoto Y, Kataoka Y, Yoshida N, Meguro H,
            Aburatani H, Asashima M and Nishinakamura R.
  TITLE     Identification of kidney mesenchymal genes by a combination of
            microarray analysis and Sall1-GFP knockin mice
  JOURNAL   Mech Dev 121 (6), 547-557 (2004)
   PUBMED   15172686
REFERENCE   8  (bases 1 to 3658)
  AUTHORS   Kitajiri SI, Furuse M, Morita K, Saishin-Kiuchi Y, Kido H, Ito J
            and Tsukita S.
  TITLE     Expression patterns of claudins, tight junction adhesion molecules,
            in the inner ear
  JOURNAL   Hear Res 187 (1-2), 25-34 (2004)
   PUBMED   14698084
  REMARK    GeneRIF: Claudin-12 is expressed at the variety of epithelial
            tissues in inner ear including Organ of Corti, stria vascularis,
            Reissner's membrane, spiral limbus, vestibular sensory epithelia,
            and dark cell area.
REFERENCE   9  (bases 1 to 3658)
  AUTHORS   Nitta T, Hata M, Gotoh S, Seo Y, Sasaki H, Hashimoto N, Furuse M
            and Tsukita S.
  TITLE     Size-selective loosening of the blood-brain barrier in
            claudin-5-deficient mice
  JOURNAL   J Cell Biol 161 (3), 653-660 (2003)
   PUBMED   12743111
REFERENCE   10 (bases 1 to 3658)
  AUTHORS   Niimi T, Nagashima K, Ward JM, Minoo P, Zimonjic DB, Popescu NC and
            Kimura S.
  TITLE     claudin-18, a novel downstream target gene for the T/EBP/NKX2.1
            homeodomain transcription factor, encodes lung- and
            stomach-specific isoforms through alternative splicing
  JOURNAL   Mol Cell Biol 21 (21), 7380-7390 (2001)
   PUBMED   11585919
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC068663.5.
            
            On Nov 22, 2023 this sequence version replaced XM_030254750.1.
            
            Summary: This gene encodes a member of the claudin family. Claudins
            are integral membrane proteins and components of tight junction
            strands. Tight junction strands serve as a physical barrier to
            prevent solutes and water from passing freely through the
            paracellular space between epithelial or endothelial cell sheets,
            and also play critical roles in maintaining cell polarity and
            signal transductions. This gene, along with several other family
            members, is expressed in the inner ear. The protein encoded by this
            gene and another family member, claudin 2, are critical for vitamin
            D-dependent Ca2+ absorption between enterocytes. Multiple
            alternatively spliced transcript variants encoding the same protein
            have been found. [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (5) differs in the 5' UTR, as
            compared to variant 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189676.140513.1,
                                           SRR12282455.12245074.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-222               AC068663.5         33556-33777
            223-312             AC068663.5         35154-35243
            313-3658            AC068663.5         39951-43296
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 2.98 cM"
     gene            1..3658
                     /gene="Cldn12"
                     /note="claudin 12"
                     /db_xref="GeneID:64945"
                     /db_xref="MGI:MGI:1929288"
     exon            1..222
                     /gene="Cldn12"
                     /inference="alignment:Splign:2.1.0"
     exon            223..312
                     /gene="Cldn12"
                     /inference="alignment:Splign:2.1.0"
     exon            313..3658
                     /gene="Cldn12"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    315..317
                     /gene="Cldn12"
                     /note="upstream in-frame stop codon"
     CDS             342..1076
                     /gene="Cldn12"
                     /codon_start=1
                     /product="claudin-12"
                     /protein_id="NP_001412601.1"
                     /db_xref="GeneID:64945"
                     /db_xref="MGI:MGI:1929288"
                     /translation="

MGCRDVHAATVLSFLCGIASVAGLFAGTLLPNWRKLRLITFNRNEKNLTIYTGLWVKCARYDGSSDCLMYDRTWYLSVDQLDLRVLQFALPLSIVIAMGALLLCLIGMCNTAFNSSVPNIKLAKCLVNSAGCHLVAGLLFFLAGTVSLSPSIWAIFYNSHLNRKFEPVFTFDYAVFVTIASSGGLFMTALLLFVWYCACKSLSSPFWQPLYSHAPGMHTYSQPYSSRSRLSAIEIDIPVVSHST"

     misc_feature    372..434
                     /gene="Cldn12"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ET43.2);
                     transmembrane region"
     misc_feature    603..665
                     /gene="Cldn12"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ET43.2);
                     transmembrane region"
     misc_feature    747..809
                     /gene="Cldn12"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ET43.2);
                     transmembrane region"
     misc_feature    864..926
                     /gene="Cldn12"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ET43.2);
                     transmembrane region"
     misc_feature    1023..1025
                     /gene="Cldn12"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P56749; propagated from
                     UniProtKB/Swiss-Prot (Q9ET43.2); phosphorylation site"
     misc_feature    1032..1034
                     /gene="Cldn12"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9ET43.2); phosphorylation site"
ORIGIN      

agaacgcacttccgcggcagcggagggagaccaaggagccgcggctcggagtgggtgagcgggatgcccgagccgtgaggagccggtaggtgtggtcagggacgctcccgtttccgagtcttttgcaagtagttgaaggtgttgcgtgaaacggcgaagcggcggcgagtcggcatttgaaaggttgcccacatgggtggacgtggaaaagtggccgaggaggtccagattatctctgctttgtgtgcagatgtgctcctgttgcatctgagttcactaagctgactttggaggatcaggcacagggaaaggactgaccatacagctcaagccctcctgccatgggctgccgagatgtccacgcagccaccgtcctgtccttcctgtgtggtattgcctctgtcgcaggcctctttgcggggactctgcttcctaactggaggaaactgcggctgatcacattcaacagaaacgagaagaacctgacgatttacacgggcctgtgggtgaagtgtgcccggtatgatggaagcagtgactgcctgatgtacgaccgtacgtggtacctgtcggttgaccagctggacctgcgtgtcctccagtttgccctgcctctcagcatcgtgatcgcaatgggtgccttgctactctgcctgattggaatgtgtaacacggccttcaattcttccgtgcctaacatcaaactggccaagtgtctggtcaatagtgcaggctgccacctggtggccggactcctgttttttctggcaggtaccgtgagcctctctccgtccatctgggccatcttttataacagccatctcaacaggaagtttgagccggtctttacctttgactatgcagtatttgtcactattgctagctcagggggtctgtttatgactgctctcctgctgttcgtttggtattgtgcatgcaagtctttgtcctctcctttctggcaaccgctgtactctcacgctcccgggatgcacacttactcacagccctattcatcacggtcccgcctctctgccattgaaatcgacattccagtagtctcacacagcacttaacagagatacaattaactgttcaaaggatgtcttgtagcctcacagccccctgcataatgagcctttttggacctgtgtacatttttcctatgttccttatcagtcagtggagccaaatttgtatatcttggtagaatgaaatgctgctagattttatgaggtacatattacattcttctaaatgtgaaccattccttttatcttgcttctgagactagaagggtctctagcctccttgttgatgtctgatcaatagcttatgtgaaaggacccatgataacactggaggcagtgaagacagcatattgaacaatgctgtttgatcttcacccgtatttcagattgtgcattgactccgatgattgaaaggtggaaggggcagtttcaaacttaagacgtttcagtcggatgaggctaggagtttgtctgtctccagaactcttccagcttgaaggtataatttcattctctctggggactaattgaataggcatcagtatactgaagattgctatcttctacctcattccagggaaaagtaaaacgcattttacatgacagagtaaagctaatatttaagcatggttaacatttggcttcttcattggtttcagatggggtcttgctgtgtagctctggctggcctagagatcactgcagaccagtgtgtactcagactttctacccgcttgcctcctgagcactaactacaaggcacacccaattacactaacaatgcgtcaggagacagtcagtatgttgcttcatttgaagcaagaatccctttccagatccccttatcaggtagtgctcatgcgctggccaagctcttagtgcctaggacaaaattgtttttcagggcgcgggagtctgttctttagtgtcagcgccaaagattcatttggtggctactatttgagtagtggctttagtctgttcagttcctgcttcagaactgatttcttgcttctgacactgcttaacgtttgagtaagtacttcagaggatacacaaataggagaaatagccatgtctatgtaggaatgttgatctaattcacctgataaccagtgggaagtctgacattatggtagctggtaagcttgtcaagcattgacccaggagtgtatttcatctgaaagatgtctttgtgacacagacagcctgtggtccaccaccagcaatagcctggacgctggaaattccctttaatcaggtctcctcccatagccgtaataatggtctcagatcatgagaatggtaagataggagagagattaaattatacctattctacctctgaagctaagatgataaattaaagaggggcaaattttaaaaatatctcttctgatgtgatatcaaatggataaaaccatgttagtactaacatgacattgaggggtgttgggtcttagcttagggatttgagctgctcatttagataatgtccacgtactagcattgtttgtaaggactgtagtacattgtgcttatagatttttacaagcagctgattttatgataacagtttctgatgcctcttttgcaaatctgagtctttctgtagagatatgtagtacttttccctaagtccaacactactgtatgtaggccaacactactgtatgtaggcaagttaatccaattgtataattttaaaacgccgtcaatacaaagcatgcacatctatggttttttaaaattacaacttctagccttttccacctctggttcttctcactcataaatggagctgagtgttggtcattccaagacagtggcagctcgaattctgagactaataatagtgttttccctttgttaattaatcttagttcttctctcactaaactgtttccagatggaattctaccatgccttggtgttagtatacagagataaaagtagggccaggtgtagtggcttttgactttaataccagcactcaggaggcagtgacaggtgcatttctggaaattctggtctccatagcaagttccaggccagtcagagctacacagtgagaacctgtctttaaaaacaattagaaaaagtggactcagcaatgagaatactagacagttttcagctcacaggattgtaactacaaacatttaagctttcttatgagaatctttcggcattatcatctatagcctcaaattactgttatgaattcactcaggaagagaaacagctgtttctgtattatctttttagtgttccagattgggcataaatagctgttttttaaagccatcaaaggaaagcacgtatttacaatgcttttgagtcactggggctgttattgagatggggtggataacttcttttgttgctaaaaagctgttgattttaaaggccttctaaggagccagttaggcaaagaaccttgaatgtgagacggtgcatagaatttctgttacccagctttgggagaagaataaacctgagattctgagttcactctgcttgtacatacgccctatgacggatagtgtgtgctgtaaaccacaaggtagaatcctcagtgggtgtgctgtgctgcctcatgtttattaaaagatgtatttttacaa

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