LOCUS       XM_066644111            6896 bp    mRNA    linear   VRT 22-JUL-2024
DEFINITION  PREDICTED: Hoplias malabaricus argonaute RISC component 1 (ago1),
            mRNA.
ACCESSION   XM_066644111
VERSION     XM_066644111.1
DBLINK      BioProject: PRJNA1136869
KEYWORDS    RefSeq.
SOURCE      Hoplias malabaricus (trahira)
  ORGANISM  Hoplias malabaricus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Characiformes; Characoidei; Erythrinidae; Hoplias.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_089800) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_029633855.1-RS_2024_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/19/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Hoplias malabaricus"
                     /mol_type="mRNA"
                     /isolate="fHopMal1"
                     /db_xref="taxon:27720"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="blood"
                     /dev_stage="adult"
                     /geo_loc_name="Brazil: Represa do Rio Monjolinho - Sao
                     Carlos, State of Sao Paulo"
                     /lat_lon="21.985889 S 47.880722 W"
                     /collection_date="2021-04-21"
                     /collected_by="Marcelo de Bello Cioffi"
     gene            1..6896
                     /gene="ago1"
                     /note="argonaute RISC component 1; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 55
                     Proteins"
                     /db_xref="GeneID:136666101"
     CDS             69..2645
                     /gene="ago1"
                     /codon_start=1
                     /product="protein argonaute-1"
                     /protein_id="XP_066500208.1"
                     /db_xref="GeneID:136666101"
                     /translation="

MEPGASGAVPMGPYPPPLQQVFQAPRRPGMGTVGKPIKLLANYFEVEIPKMDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQLFGDRKPVYDGKKNIYTVLALPIGSEKVDFEVTIPGEGKDRIFKVSIRWLAKVSWRLLQETLVSGRLQVPLDSVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKTLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNANFNLDPYIQEFGIKVRDDMAEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKSERIGKSGNIPAGTTVDTSITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHVSGQSNGRDPQALAKAVQIHHDTLRTMYFA"

     misc_feature    171..563
                     /gene="ago1"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    591..743
                     /gene="ago1"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    744..1106
                     /gene="ago1"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(900..902,945..947,987..989,999..1001,1053..1055,
                     1074..1076,1080..1082)
                     /gene="ago1"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1239..2516
                     /gene="ago1"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1650..1652,1662..1664,1698..1709,1716..1718,
                     1740..1742,1749..1751,1761..1763,1773..1775)
                     /gene="ago1"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1854..1856,1860..1862,2070..2072,2484..2486)
                     /gene="ago1"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

gaggaaagaggcgagggaagcagggccgtggtgctggagccccggggctttagtgaaaagtgaatgggatggagccgggagcatctggcgctgtgcccatggggccgtacccccctcccctgcagcaggtgttccaggcgccccgtcggccgggcatgggcaccgtggggaagcccatcaagctgcttgccaactactttgaggtggagatccccaagatggacgtctatcactatgaggtcgacatcaaacctgacaaatgtccacgaagggtcaacagggaggttgtggagtacatggtccagcatttcaaaccgcagcttttcggtgaccggaagccagtatacgacggcaagaagaacatatacacagtcctggctcttcctatagggagcgaaaaggtggattttgaggtgaccatcccgggtgaaggtaaagaccggatctttaaggtgtctatccgctggctggcgaaggtttcgtggcggctgcttcaggagacactggtcagtggccggctccaggtccccctggactctgtccaggcactggatgtggccatgagacatctggcctccatgaggtacactccagtgggtcgatccttcttctctccacctgaagggtattaccatcctctgggaggggggagggaggtgtggttcggcttccatcagtccgtacgacccgccatgtggaagatgatgctcaacattgatgtgtcagccacggctttctacaaagctcagccggtcattgagttcatgtgcgaagtcctggacatccgcaatattgacgagcagcccaagaccttaacagactcccaaagggtacgattcaccaaggagatcaaagggctgaaggtagaggtcacacactgcggtcagatgaagagaaaatacagagtgtgtaacgtcacgcgtcgtccagctagtcaccagacgtttccactgcagttagagagcggtcagacggtggagtgcacggtggcacagtattttaaacagaaatacaacctgcagctcaaatacccccatttgccctgtctgcaggtgggacaggagcagaaacacacttacctgcccctcgaggtgtgtaatatcgtggccgggcagaggtgcatcaaaaaactgacagataaccagacgtccaccatgatcaaagccacagcgcgttcagcaccagacagacaggaggagatcagcagactgatgaagaacgctaattttaatctggacccttatattcaagagtttgggattaaagtgagggacgacatggcggaggtgaccggtcgagttctcccagccccaatcttacagtatggaggcaggaaccgggccatagccacgcccaatcagggagtctgggacatgagagggaagcagttttacaatggcatcgagatcaaagtgtgggctatcgcctgcttcgctccacagaaacaatgcagagaggaggtgctcaagaacttcacagaccagctgcgtaagatctctaaggacgctgggatgccgattcagggtcagccgtgtttctgtaagtacgcgcagggagctgacagtgtggagcccatgttcagacacctgaagaacacctactccggcctgcagctcattatcgtcatcctgcctggaaaaacgcccgtctatgcggaggtgaagcgtgtgggagacactctcctgggcatggccactcagtgtgttcaggtgaagaacgtggtaaagacgtctccacagacactctccaacctctgcctcaaaatcaacgtcaaactcggcggcatcaacaatatcctggttccgcaccaacggtcggcggtgttccaacagcctgtcattttcctcggagcagatgtcacacatccaccagcaggggacgggaaaaaaccctctatcacagctgtggtgggcagtatggatgcccacccgagtcggtactgtgctacagttcgagtgcagagacctcggcaggaaatcatcgaggatctttcctacatggtgcgggaactcctcatccagttctacaagtccacccgcttcaagcccaccaggatcatcttctacagggacggcgttcccgagggtcagctcccacagatcctgcactacgagctgctggccatccgtgacgcctgcatcaagctggagaaggactaccagccgggcatcacctacatcgttgtgcagaagcgccaccacacacgcctcttctgcgccgacaagtcggagaggatcgggaaaagtggcaacattccagccggcaccacagtggacaccagcatcacacacccgttcgagtttgatttctacctgtgcagtcacgcagggattcagggcaccagtcgaccctctcattattacgtgttgtgggacgacaatcgcttcacagcggacgagcttcagattctcacgtaccagctctgtcacacgtacgtccggtgcacgcgctccgtctccatcccggcgcccgcctactacgcccggctagtggccttccgcgctcgctaccacctggtggacaaagagcacgacagtggggagggcagtcacgtctctggtcagagtaacggtcgggacccccaggctctggccaaagccgtgcagattcaccacgacaccctgaggaccatgtacttcgcctgagggatctccactacacgccctggtcaccctccagtggcctcaagacactcctgacctccctgcgctcgagcaagtacacacttcagcaccaaaactacaaatcccaaaaaccccaaacagtactgaatccacgtggagcgccctgacctcaccgcatttttttaaaaacacgttgacattgctcctttttgtttgttttttgttttctttttatttggaagtatgttgtggtatactaccttcacgctcctatacatctgtatgttcatatgcaatatggaggagagggatagacccgaggatttgactgccaccacgacgctgcctcgtttcagcacctgtccagcaggccgctctccagtgtccagctcctagccctgaagtggacaggacattttgttatgttcttttttttcccccctcctcctcctctcgaagtacactattttttaatgttctcagatttgtttttatttttatttttaaaacagcctttgaagaatgaacactacgttagcacttagagttagcttagacacagtgagcccggcgtctcctgcagccaaatgcgcatcccaaaaaaaaaaaataaaagaagtggtcaggaatgagccgtcgccacggatgccttcggtgcgcagtgtagccgatcgtgttttagacacgcctccatagtcacacagttagcgtgagcatagatggactgtcgagtgcgtttacgtaagttccttacttttctatcagcacaccgaaaccagcattaacaaaccagccaaacgtgatgccaaccgttcagcttcgaggaggcacttatctttctacaccctgcatctagtcgatatgcttttattttctgaaaggatgaacggactggtcataggaaaaacagagaacgtgcttgttctcttgtcacagatctgcacaggtttttgatgacttcttatgtcccacacacacacaccagggatctgctgcccaaccctagtccaatagggctacgcaagaaattgggtttggggccagaatttagcacaatttttatgttatgatatgtttcttgttcttgccccagtccaaatatagaaccaatgctgcaaaggtgaccttaagatcattgttttttaatgacatcacaggttttaaagctcctattcataaatcagtctgttaccttgtgtagcttcaggtccagttcagcgaacaggtttcagatttagagtgttatgatgcttttccactgcatggtacctactctacatgactatactggactctagtcttggtttgattcctggttcctttttgttactacaactgtgccccccccctccaagtgtagacgctgcaatacccagtctctaacgggaacagcaccacaacaacggcagtggaaacgttaagcgttgtttactcatcgtgtattggcgtgttgttgttgtttcgtcatcagttcagacagtcaggagagtttgttccttccttgttgcaacgtccatctctccaccgatccgaggagcgtttgaacctcttcaaaagaccacggagtaaaacatgtgatctctgctgcagctggagattttacagatggggagtttgtgctggacgtctgtattttgtggggtagagtaaggattctggacaatcacctcacagtttagtccctacacggcccgcttggaaccacgagagaacagggactaaaaaaaagaacccagttccaggaccaaaattacccgatggaaaagcaaaacaagcagagtagagtagataccatgcagtggtaaagtgccattagctgtaaaattagtctcatagcctttgggctcagacccaagtctagggctgggcagaccttacaaacacacacacactctttcaaataagtatgtggccaacggtttatgagcaccttctcatccggtatttattctcggcacgtgtaggcgatgagggctcaattgctgctgaacactatctactctactcctctgggaaggctttacactacacgttggaatgtttcctgtgaagaattgatgttagagcattcgtgagcaaagatactgatgtttgatgattaaattctacactccagcccatcccaaagcaatggatagagtgccatcgctccagagaacatagatccactgctgcactgccctatttaacctacacttggcattgaacatagtggccttaggctcgtgtgtacctgcttttctacggtgcacaattgagtggccacaatggctgtaccttaaatagcctaaaataagagttcacgaaagacgaggtgtcgtcagattgttggatgtgtggtcgtacgtctttacacgtggtgcacaaatggggggaaatgcagtgtctgtactgttcacaaaatgctggcttccactgatctctgttcacgaaccagtacaaggtcaatgtataattaagagagccaccaagcagtgaagtttcttgttcaaacacttcctcacaccttataggatgtgacattcttctccttttctacaccaactccctccctccttgacattgttctcgcccacaaacgcagtttcatctctgtacctacccccaccaccttctcctgcgtagagatatggaagccctgcgtgtacggtcaaccgcagcccacttccctctccatagaaacgcaatgtcatgctttacaaggccccacccacattctcctgctcctctatggtgctcacgatagatgaacacccactctctttctcacacacacacacacacctgcctccttatatctacatccttagcctggttattattattttttacaagtctgtcaaatgtgtactacgtacacgggcattgaagaaatggaccttgtttattatttatttttatttttcatttttttaaaactccaactgaaggacaccttcatccagccaagcccttgctgtggttttacgatgaaatgcatgttttaatcaaacttgtgttgtgactggacgcttagtaactatgccacactcctccagacggacatgcccttatcctctcatttatttcatgtcatacactcctttatcggttgtaaaatgcacttctcgtccctcttgagagtctccagtaatggctatcgtcgtccacaagggggtactacagaggacagcttaagctcctttaaaatggaaaaaagaaatgtcacttgcatatggcccatcgacacctggcatctgaagcggtgttggcgttgtagtgtgtgtttcatgaccatataaggaaatctgagtcgaagctggatttttttatgacagaaatgaatgagatttttagaagatattacagtaaaaatgttcaaaatcagaaacattatgctgtatatcaaattctccccaagatcctgtgcatttcaaggttttaaaggagtcaaaatatgaatttttgccacgtgagctaacacatttatgcaccaagcatcataacccactgtaataacccacacaaaccagcaagattcaaaagtggtagcctccatattttgtagctgttgatgttgtataatgctgtttaagatcacaagtagacctgctatagagtggcatctcttccagcacaataaagttaggttacttgtagcattagtgtagcattattaatattttgactattaaaccactcaatcccaaggatctctttgcgtgaaagatgtcacatcccattcatttctgtcatagaaaagttcaccctagagttggaacgcttatctgggcatgacccggacttcagtggactattgaaattcaaagggatctggagttgtaacggttagtcacacttcctttttgaatcacctttttacaaaatgcataataagaactgggttcctattccatttcacagaaccgacatcaagataataatgtatttataccttgcaatgtaatggccttagattgttaggcctgtgtaattggggctgcgtcagacctgtctgatgaacccaggactaaacgtcatgatgcagtacttaattctcagaagccacgggcgcctagtgggtgaacgacactaacgcaaaaaaaaaggcgcaacacaaaaaaaaaaagagagagagggaactggagaataaacttcactcagcctcactgtcaaagcaatataatgccactgaaaaagaacggtccttatcatagcacttcccaatagactgattggaaggacacaaagttattttgcctctgtatgtaatagtgtgggggatgtgttggg

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