LOCUS       XM_066625639            6629 bp    mRNA    linear   VRT 22-JUL-2024
DEFINITION  PREDICTED: Tiliqua scincoides argonaute RISC catalytic component 2
            (AGO2), mRNA.
ACCESSION   XM_066625639
VERSION     XM_066625639.1
DBLINK      BioProject: PRJNA1136870
KEYWORDS    RefSeq.
SOURCE      Tiliqua scincoides
  ORGANISM  Tiliqua scincoides
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Lepidosauria; Squamata; Bifurcata; Unidentata; Scinciformata;
            Scincidae; Egerniinae; Tiliqua.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_089824) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_035046505.1-RS_2024_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/19/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Tiliqua scincoides"
                     /mol_type="mRNA"
                     /isolate="rTilSci1"
                     /db_xref="taxon:71010"
                     /chromosome="4"
                     /sex="male"
                     /tissue_type="heart muscle"
                     /dev_stage="adult"
                     /geo_loc_name="Australia: South Australia, near Adelaide"
                     /lat_lon="34.08575 S 139.05331 E"
                     /collection_date="2019-10-01"
     gene            1..6629
                     /gene="AGO2"
                     /note="argonaute RISC catalytic component 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 42 Proteins"
                     /db_xref="GeneID:136649184"
     CDS             57..2660
                     /gene="AGO2"
                     /codon_start=1
                     /product="protein argonaute-2"
                     /protein_id="XP_066481736.1"
                     /db_xref="GeneID:136649184"
                     /translation="

MYPAGGAAPAASAPVLATPSLLPPPIQAYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKAQIFGDRKPVFDGRKNLYTAMPLPIGREKVELEVTLPGEGKDRIFKVAIKWMSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPFVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA"

     misc_feature    321..578
                     /gene="AGO2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    606..758
                     /gene="AGO2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    759..1121
                     /gene="AGO2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(915..917,960..962,1002..1004,1014..1016,1068..1070,
                     1089..1091,1095..1097)
                     /gene="AGO2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1254..2531
                     /gene="AGO2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1665..1667,1677..1679,1713..1724,1731..1733,
                     1755..1757,1764..1766,1776..1778,1788..1790)
                     /gene="AGO2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1869..1871,1875..1877,2085..2087,2499..2501)
                     /gene="AGO2"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

cgagagggagcgagggcctcccgccgccttcagcgtcgccggccgcagccgcagccatgtatccggcggggggagcggcccccgccgcctcggcccccgttcttgcaacaccatcactgctgccacctccaatacaagcatatgcatttaaacctccacctcggcctgactttggtacttcagggagaacaatcaaactgcaagccaacttttttgaaatggacattcctaaaattgacatttaccattatgaattggatataaaaccagaaaagtgccctaggagagttaacagagaaatagttgaacacatggtccagcactttaaagcgcaaatatttggagatcgtaagccggtatttgatggaagaaaaaacctctatacagctatgccgcttccaatagggcgagagaaagtggaattggaagtcacgttgccgggagaagggaaggacagaatatttaaggtggcaataaagtggatgtcatgtgtgagtctgcaggctttacatgatgctctttctggtcgtcttccaagtgttccttttgaaaccattcaggcactggatgtagtaatgaggcatctgccttccatgaggtatactcctgtgggccggtcctttttcactgcatcagaagggtgctctaatcccttgggtggtggcagagaagtttggtttggattccatcaatcagttaggccttcactgtggaaaatgatgctcaacattgatgtatctgcaacagcattttataaggcacaaccagtaattgagttcgtatgcgaagttttggattttaaaagtattgaagaacaacagaaacctctgacagattcccaaagggttaagtttaccaaagaaattaaaggtctgaaggtggagatcactcactgcgggcaaatgaagagaaagtacagagtatgcaatgttaccagacgaccagcaagtcaccaaacatttccacttcagcaggagagtggacagacagttgaatgcactgtagctcaatattttaaggacaggcacaaattggttttacgataccctcatcttccttgtttacaagttggacaagagcagaaacacacataccttcctctagaggtgtgcaacatagtagcaggacagagatgtataaagaagctgacagacaatcaaacttccactatgataagagcaaccgctagatcagcaccagaccgccaagaagaaattagcaaactgatgcgaagtgcaagttttaataccgatccttttgttcgtgaatttggaataatggtcaaagatgaaatgactgatgtgactggtcgggtcctgcagcctccttctatcctctatggaggaaggaataaagcaattgctacaccagtgcaaggagtttgggatatgaggaacaagcagtttcacactggaattgaaatcaaggtctgggcaattgcttgctttgccccacagcgccagtgcactgaagttcaccttaaaaccttcacagaacaattgaggaagatttcaagagatgccggaatgcccatccagggtcaaccctgtttctgtaaatatgctcaaggagcagatagtgtggagccaatgttcaggcacctaaagaacacctatgctggtttgcagcttgtagtagtcattctgccaggaaaaactccagtttatgcggaagtaaagcgtgttggtgacactgtattgggcatggccacgcagtgtgtgcagatgaaaaatgtgcaacggacaacacctcagaccctctccaatctgtgcttaaagatcaatgtcaaactgggaggagtaaataatattttgctgccccagggaagacctccagtatttcagcagcctgtaattttcctgggtgcagatgtcactcatccaccagctggagatggcaaaaaaccttccattgctgctgttgtaggtagcatggatgcccacccaaatcgatactgtgccactgtgagagtccagcaacatcgtcaagaaattatccaagacttggctgccatggtcagggagctgttgattcagttttacaaatccacccgattcaaaccaactcggattattttctacagagatggtgtttctgaggggcagttccaacaggtacttcatcatgaattactggctatccgagaagcatgtattaagctagaaaaagactaccagcctggaataacattcattgttgtgcagaaaagacaccacaccaggctcttctgtacagacaaaaatgaaagggttggtaaaagtggaaacattccagcaggtactacagtggacacaaaaatcacccatccttcagagtttgacttctatctatgtagtcatgctggtattcagggaacaagcagaccttctcattaccacgttctctgggatgacaatcgtttttcttcagatgagcttcaaatactcacctaccaactgtgtcatacgtatgtacgctgtactcgttccgtttcaattcctgcaccagcatactacgcacacctggttgcctttagagccaggtatcatctggtggataaagaacatgatagtgctgaaggaagtcacacctctggtcaaagcaatggcagagatcatcaagcacttgcaaaagctgttcaggttcatcaggatacgttacgcaccatgtactttgcttgacatgttgcagtgatttaaataactcggttcagagttggattcacaggagaccagctgcacttagaccaacagttgcccaagctatagcgacagccagcaatgagtgatacctctttattttattaggtttgaattgtattttattttccattttaaaagaagccttccgtccaaaatttcagacttgatttcaaactgctgacattacaaaaattgcacagctattagaagtgtggatttgccaaaagcaaagagctttttaggagaaaaagaaaaaaaatcaaaccccatattttgtaacagaaacaaaagctgtgatctcagggcttaaacacttcattaagttatgttgttctccttaaatatttgtttttaactaatctgaaaagaaccagttaagatcagttttaatggactgatataaagctatgaatttgctttggtttctgaaaagaaaacagagcgctaatgtgggtaccattgaaaagcttgatcattctggatgaattcatttttttaatttttaatttttatttgtaattgcaatgcaaaaggagagaaatctggtttatccttggctgtcttatttcagtgaacagtctgtatgggtctaagggcaaaagtattttctgaagttcttgaatgtctttcattgtgctgcgtttaaatttttcatagaatgaattatggaaatatttcaggaagaaacaaggagattattatctatactttttattaggcccattgaaagataacaaagtgtgccattaaaaaaagtagttttttttcttttttaatcaattttgatgactaatcatggagtttgtcctctaggactgtgacacttaattttccaaagtttttctaaagaatacgggtctgtggtgatacagtacaatctttttcactgttatgtttgaattaatgtaaaatttatatatgtttcacagtattaacatgatagactagatgctatttatttttatttactaaggcgtgcttaacatgtttctgttaacatatcaagaatgctttggatattggaaatgaaaaccttatcatggggacactagtgattttgggtgcagttcaccaatcattgttttcataagttctctattcttctcaagagggtttgcatggaagaccgctcatatccccttgaaattaatagaggataacttgtggtgatgttttggtgtattgcacctttcaaaagcaggaatatgcaactgacttagagtgatagcattgagagatttatgtatacacatatataatttagcttgtacctggaagactttgaagagttaattcaacagagtagagctaacacttctgtagtttaggcatggacacacaagaacagtccttgagttataagcacacacaaaaaacgatctacctgtttgatcatctgggatatagaagtggaagtatgtacatgagggagaaggtgtccatcttatgtataagggaaactcagggtccagtcctatccaattttccagcactgatgcagcctcaattcagccttgaggtaagggatcaaacgttccctttattgaggaggtctctgttattgctttccccaccaccacaggatgaagtgcatgccccattggcatggcctcatcagcactggaaagttggatagaattggcctctgagtctggagggtttgccattctatttggagaagcattaattcaaactgagacctactgagaacaaaaacttctgtacatttgcacagagttcatataaatgattattcacagacacccattgcactaatgacaacaggaacactaccaagatatagaaaaaaataagtttttctcctaatctgatctcgttggacttttaaatcagacaaaacagcaagaaaatgttacatatcttttaatttttgaaacattcctaagatttcttagaatttattttctttctcctggatagccaatgttataaaattttagtgcccatttctccattctgagaattgggaacgaggggttaggctgtgctaggcttattatggtggtatagaactgatagttgatacctgtgttaaaaatgcttgaattgttgcctattaaattttagggatctgacttctgcacctagtaaacagtgcctcatgttcccaaacagatgtggcttgaacatcaaggttttgaggcaagacagtgtcacgtgcgtttattctttttttaaagtgtgtgtgtggtgttgttttttttaatctggacattgcagttccagcagcttgataagtaaataggatcccgccagggtctctatcattatggaaagtaggttgtcacatccttttcttaacacgtagtgtgtagctcctttgtggatttgttgcactttccagcttggagagatgttgaaatgtgaaggtcatgttcaagtttgactacgactcagtaggtgaacacgctgatcttttaaaagcttatctcctgcttttgaagttatgtcctgattagccatctgtcttaatcaggcacttgagatgccaaccattcattctgtccttgcttttctatcaccttgaatctaaatctggcaaaaagtgataggctaggatgtgaatcttagccagaactgagtggcctggttatcaaggagccctgtctcatggctgttgttgactagcattttcaacccttccattgcattgccagctccagaatataaatgtgtgccacagttgttgtcaggtgcttgtgtaggtgactcttctgtagatagttctccaaagaattaatgttgtcttgtcactggcatgtgcaggaactttgtgtgtgtgcgtgtgtgcgtgcgtgtgtgtgcctttctgtgcagaagaaatatgatggttttgtgaagattaggtatgattcttgggccagaaaggagaaatctgtgtgtttcgttatcttcccttttcttttaatcttgtatactgtattcttaaaaatgacttttgctttttctgtgctagatgcatggcaaactagtgtctaccacattttaacatccggggaggggggagggattgtaaaacgtgttttgaacagctgacaatgcactttgaaaaagtagggcactctaaatatgcacaggaattgctttttataatatatgcataaaccaaaactccaaattgaaacaaagaatacacctttctcaatagctgtttactgcagtggctcttgtaagcacagcaatatctcaggtgaagaatttcatacatttttatccaaaatatcctctgtggagagcataggagaatagtttacaaaaactgaggcggagtccaggatagttacgtgtacctggggatccaatctgttaccttttatctttaacatcgcttcataacaaaactggttttaacccacatctgcccatatgccctacaggcagcacctatatgttgcatgtgtagacccgcaggcaaagatggctgtctttttggctaatttggctttaaagaaaatgcctctctgtccactagtgaggaacctggatctgatcatcacatttgtagtcagtggaaggaacagtatgcatttttagtgtcatggtcttattaatttgtcgtaattgataggagttggcactggtatgtgcagtttgtagccaaagaggtgttgcaaaattctattccctgggattgctgcagtttttgtcttacttcatcctcaagggttagactgtgctggatgcttacctgtgctattgcctggactattctgcttggtgctttaaactacatgcaacacctctttaatatggaagaatagagccgatagctttgtcaaagccagagctgtgttgttggtttcacactgccaagtcagaaccctcatcttgaaggaagcttgttggaagtgttagcaaaacaaaacacccacaagatttgagtctgccaggttaatttagagtttcctgctttatttgttgtaaaat

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