LOCUS       NM_128071               3348 bp    mRNA    linear   PLN 20-OCT-2022
DEFINITION  Arabidopsis thaliana casein lytic proteinase B4 (CLPB4), mRNA.
ACCESSION   NM_128071
VERSION     NM_128071.3
DBLINK      BioProject: PRJNA116
            BioSample: SAMN03081427
KEYWORDS    RefSeq.
SOURCE      Arabidopsis thaliana (thale cress)
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
            Camelineae; Arabidopsis.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Lin,X., Kaul,S., Rounsley,S., Shea,T.P., Benito,M.I., Town,C.D.,
            Fujii,C.Y., Mason,T., Bowman,C.L., Barnstead,M., Feldblyum,T.V.,
            Buell,C.R., Ketchum,K.A., Lee,J., Ronning,C.M., Koo,H.L.,
            Moffat,K.S., Cronin,L.A., Shen,M., Pai,G., Van Aken,S., Umayam,L.,
            Tallon,L.J., Gill,J.E., Adams,M.D., Carrera,A.J., Creasy,T.H.,
            Goodman,H.M., Somerville,C.R., Copenhaver,G.P., Preuss,D.,
            Nierman,W.C., White,O., Eisen,J.A., Salzberg,S.L., Fraser,C.M. and
            Venter,J.C.
  TITLE     Sequence and analysis of chromosome 2 of the plant Arabidopsis
            thaliana
  JOURNAL   Nature 402 (6763), 761-768 (1999)
   PUBMED   10617197
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 3348)
  AUTHORS   Krishnakumar,V., Cheng,C.-Y., Chan,A.P., Schobel,S., Kim,M.,
            Ferlanti,E.S., Belyaeva,I., Rosen,B.D., Micklem,G., Miller,J.R.,
            Vaughn,M. and Town,C.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Plant Genomics, J. Craig Venter Institute,
            9704 Medical Center Dr, Rockville, MD 20850, USA
  REMARK    Protein update by submitter
REFERENCE   4  (bases 1 to 3348)
  AUTHORS   Swarbreck,D., Lamesch,P., Wilks,C. and Huala,E.
  CONSRTM   TAIR
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2011) Department of Plant Biology, Carnegie
            Institution, 260 Panama Street, Stanford, CA, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by TAIR and Araport.
            This record is derived from an annotated genomic sequence
            (NC_003071).
            
            On Sep 12, 2016 this sequence version replaced NM_128071.2.
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Arabidopsis thaliana"
                     /mol_type="mRNA"
                     /db_xref="taxon:3702"
                     /chromosome="2"
                     /ecotype="Columbia"
     gene            1..3348
                     /gene="CLPB4"
                     /locus_tag="AT2G25140"
                     /gene_synonym="CASEIN LYTIC PROTEINASE B-M; casein lytic
                     proteinase B4; CLPB-M; F13D4.100; F13D4_100; HEAT SHOCK
                     PROTEIN 98.7; HSP98.7"
                     /note="Encodes ClpB4, which belongs to the Casein lytic
                     proteinase/heat shock protein 100 (Clp/Hsp100) family.
                     Targeted to the mitochondrion, also referred to as ClpB-m.
                     Transcripts of ClpB4 accumulate dramatically at high
                     temperatures, suggesting that it may be involved in
                     response to heat stress."
                     /db_xref="Araport:AT2G25140"
                     /db_xref="GeneID:817052"
                     /db_xref="TAIR:AT2G25140"
     CDS             206..3100
                     /gene="CLPB4"
                     /locus_tag="AT2G25140"
                     /gene_synonym="CASEIN LYTIC PROTEINASE B-M; casein lytic
                     proteinase B4; CLPB-M; F13D4.100; F13D4_100; HEAT SHOCK
                     PROTEIN 98.7; HSP98.7"
                     /inference="Similar to RNA sequence,
                     EST:INSD:ES037342.1,INSD:BX840937.1,INSD:EH863962.1,
                     INSD:ES165519.1,INSD:AV565945.1,INSD:BP595317.1,
                     INSD:ES030218.1,INSD:BP593416.1,INSD:EL045476.1,
                     INSD:BP561843.2,INSD:EH919876.1,INSD:BX836593.1,
                     INSD:ES032698.1,INSD:CD568341.1,INSD:EL984194.1,
                     INSD:EL977945.1,INSD:EH818594.1,INSD:AV822355.1,
                     INSD:CB257509.1,INSD:CD568342.1,INSD:EG471857.1,
                     INSD:EH852152.1"
                     /inference="similar to RNA sequence,
                     mRNA:INSD:AY070722.1,INSD:BT002223.1"
                     /note="casein lytic proteinase B4 (CLPB4); FUNCTIONS IN:
                     nucleoside-triphosphatase activity, ATPase activity,
                     nucleotide binding, ATP binding; INVOLVED IN: response to
                     heat; LOCATED IN: mitochondrion, chloroplast; EXPRESSED
                     IN: 22 plant structures; EXPRESSED DURING: 13 growth
                     stages; CONTAINS InterPro DOMAIN/s: Clp ATPase, C-terminal
                     (InterPro:IPR019489), ATPase, AAA+ type, core
                     (InterPro:IPR003593), ATPase, AAA-2 (InterPro:IPR013093),
                     ATPase, AAA-type, core (InterPro:IPR003959), Chaperonin
                     clpA/B (InterPro:IPR001270), Chaperonin ClpB
                     (InterPro:IPR017730), Chaperonin ClpA/B, conserved site
                     (InterPro:IPR018368), Clp, N-terminal
                     (InterPro:IPR004176); BEST Arabidopsis thaliana protein
                     match is: casein lytic proteinase B3 (TAIR:AT5G15450.1);
                     Has 30488 Blast hits to 27328 proteins in 3166 species:
                     Archae - 421; Bacteria - 19864; Metazoa - 870; Fungi -
                     613; Plants - 740; Viruses - 7; Other Eukaryotes - 7973
                     (source: NCBI BLink)."
                     /codon_start=1
                     /product="casein lytic proteinase B4"
                     /protein_id="NP_565586.1"
                     /db_xref="Araport:AT2G25140"
                     /db_xref="GeneID:817052"
                     /db_xref="TAIR:AT2G25140"
                     /translation="

MALRRLSKSVSSAIKAQYTLSRPSPLLRSRSLSSSPHYTSIGRPTNSFIGKINNSSITHATTTHGQLFPLSSPRRFCTTTAQVNQNEFTEMAWEGLINAFDAARESKQQIVESEHLMKALLEQKDGMARKIFTKAGIDNSSVLQATDLFISKQPTVSDASGQRLGSSLSVILENAKRHKKDMLDSYVSVEHFLLAYYSDTRFGQEFFRDMKLDIQVLKDAIKDVRGDQRVTDRNPESKYQALEKYGNDLTEMARRGKLDPVIGRDDEIRRCIQILCRRTKNNPVIIGEPGVGKTAIAEGLAQRIVRGDVPEPLMNRKLISLDMGSLLAGAKFRGDFEERLKAVMKEVSASNGQTILFIDEIHTVVGAGAMDGAMDASNLLKPMLGRGELRCIGATTLTEYRKYIEKDPALERRFQQVLCVQPSVEDTISILRGLRERYELHHGVTISDSALVSAAVLADRYITERFLPDKAIDLVDEAGAKLKMEITSKPTELDGIDRAVIKLEMEKLSLKNDTDKASKERLQKIENDLSTLKQKQKELNVQWEKEKSLMTKIRSFKEEIDRVNLEIESAEREYDLNRAAELKYGTLLSLQRQLEEAEKNLTNFRQFGQSLLREVVTDLDIAEIVSKWTGIPLSNLQQSEREKLVMLEEVLHHRVIGQDMAVKSVADAIRRSRAGLSDPNRPIASFMFMGPTGVGKTELAKALAGYLFNTENAIVRVDMSEYMEKHSVSRLVGAPPGYVGYEEGGQLTEVVRRRPYSVVLFDEIEKAHPDVFNILLQLLDDGRITDSQGRTVSFKNCVVIMTSNIGSHHILETLRNNEDSKEAVYEIMKRQVVELARQNFRPEFMNRIDEYIVFQPLDSNEISKIVELQMRRVKNSLEQKKIKLQYTKEAVDLLAQLGFDPNYGARPVKRVIQQMVENEIAVGILKGDFAEEDTVLVDVDHLASDNKLVIKKLESNASAEEMAA"

     misc_feature    455..3052
                     /gene="CLPB4"
                     /locus_tag="AT2G25140"
                     /gene_synonym="CASEIN LYTIC PROTEINASE B-M; casein lytic
                     proteinase B4; CLPB-M; F13D4.100; F13D4_100; HEAT SHOCK
                     PROTEIN 98.7; HSP98.7"
                     /note="ATP-dependent Clp protease, ATP-binding subunit
                     ClpA [Posttranslational modification, protein turnover,
                     chaperones]; Region: ClpA; COG0542"
                     /db_xref="CDD:440308"
ORIGIN      

tttaggtattagcctgcctatagaaaaacccagtggtggatcatcactggaaacttctcgatgaaatgactttcttctacaaacttctttttccctcttctccaagcttacgcttgtctccatgtaaaaaacctccgtcgtcttcttcctcctccttcataaccctaaaccaaaaaccctcatttccttcaatcttcttactcacatggcgctaaggagattatcaaagtctgtttcgtcagccatcaaagctcaatacactctctcgagaccctctcctctcctccgttctcgctctctctcttcctctcctcactacacctctattggccggccaacaaattcattcatcggaaaaatcaataactcttctatcactcatgcgactactactcatggtcaactcttccctctttcttctccgcgacgcttttgtactaccacagctcaggttaatcaaaatgagtttacggagatggcatgggaagggcttatcaacgcctttgacgcggctcgtgaatcgaaacaacagattgtggaatctgaacatttgatgaaagctcttcttgagcaaaaagatggtatggctagaaagattttcactaaagctggaattgacaattcctctgttcttcaagctacagacttgttcatatctaaacaaccaacggttagtgatgcaagtggtcagagactagggtcatctcttagtgttatattagaaaatgcaaagaggcataagaaagatatgttggatagttatgtatccgtggagcactttttgttggcttattattcagatacaaggtttggacaagagtttttcagggacatgaaacttgatattcaagtgctaaaggatgctattaaagatgtacgcggtgatcaacgagtaactgatcggaatcctgaaagcaaatatcaagcacttgaaaagtatggaaatgatttaactgagatggctagacgagggaagctggatcctgtcattgggcgggatgatgaaattaggcgttgcatccagatattatgtaggagaacaaaaaacaatccggttattattggtgaacctggtgtagggaaaactgcaattgcagaggggttggctcaacgaattgtgcgaggagatgtccctgaacctctcatgaatcgaaagttgatatctctcgacatgggttcacttcttgctggtgccaaatttcgaggagattttgaggagaggttgaaagcagtaatgaaggaagtatctgcttccaatgggcaaacaatcttgtttattgatgagatccacactgttgttggtgcaggagctatggatggagcaatggatgcaagcaacctcttgaaaccaatgcttggacgaggtgaactgagatgcattggtgcaactacactgaccgaataccgcaaatatatagagaaagacccagctctagagcgtaggtttcaacaagtgttatgtgttcagccctctgttgaagatacaatctcgattcttcgtgggttaagagagcggtacgagttacaccatggcgttactatatcagatagtgcccttgtttcagcagcagttcttgcagaccgctacatcaccgaacgctttctgccagacaaagctattgatcttgttgacgaagctggtgcaaagctgaagatggagatcacttctaaaccaactgaactagatggaatagatagagctgtgatcaaattggagatggaaaagctttctcttaaaaatgatactgataaagcatctaaagaacggctacagaagattgagaatgatctgtccacgctcaaacagaaacagaaagaactcaatgtccaatgggagaaagaaaagtcgctcatgacaaaaatacgttcgtttaaggaagagattgatcgagttaacctcgaaattgaatctgctgaacgtgaatatgatctaaaccgagctgctgagctgaagtatggaacacttttgtccctccaacgccaattagaagaagctgagaagaatctcacgaatttccgacagtttggacagtcactactccgagaagtggttaccgatcttgacatagcagaaatagtaagcaagtggactggtattccactatctaatcttcagcaatcagagagagagaagttagtgatgttggaagaagtgctccaccatagagttattggtcaagacatggctgtaaaatcagtagcagatgctatccgacgttcaagggctggtctttctgatcctaaccgcccaatagctagtttcatgttcatgggtccaacaggtgttggtaagaccgagcttgccaaggctctagcgggatacctttttaacactgaaaatgcaatagtgagggtcgatatgagtgagtacatggaaaaacactcagtctcacggcttgttggtgcaccacctggctatgtcggttatgaagaaggtgggcagttaactgaagttgttcgcaggagaccttactcggtggttctgtttgatgagatcgagaaagcccaccctgatgtctttaatatcttgctacaactgctagacgatggaagaataactgattctcaaggaaggaccgtaagtttcaaaaactgtgttgtaattatgacgtcgaatataggatctcaccacatacttgagaccctccgcaacaatgaagacagcaaagaagcagtttatgagataatgaagcggcaagttgtggagttagcaagacaaaatttcaggcctgagttcatgaacagaattgacgagtacatagttttccagccgctggattctaacgaaatctccaaaatcgtagagttacagatgagacgggtgaagaatagcttggaacagaagaaaatcaaacttcagtataccaaagaggcagtggatctccttgctcagcttggctttgaccctaactacggggcaaggccggtgaagcgagtgattcaacagatggtggagaatgagatcgctgtcgggattttgaaaggagattttgcagaagaagataccgttcttgtcgatgttgaccacttggctagtgacaacaaattggttatcaagaagcttgagagtaatgcttcagctgaagaaatggccgcttaattgcacaaacaaaattagacgattcggggtttagcgtttataactcttttgttctgtcttttgtagattcgtactttagtgttgctctctcttctaactcgtacggaatgtaatgttttgaggattatcttttgccgacaaaagtaaatgggaacacaccgacttattcctccgttagcatttgaaattatgaactttatggagcttttaaggactcacgtaccaatgtggtactattagaatttgtc

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