LOCUS       NM_121549               3186 bp    mRNA    linear   PLN 20-OCT-2022
DEFINITION  Arabidopsis thaliana casein lytic proteinase B3 (CLPB3), mRNA.
ACCESSION   NM_121549
VERSION     NM_121549.3
DBLINK      BioProject: PRJNA116
            BioSample: SAMN03081427
KEYWORDS    RefSeq.
SOURCE      Arabidopsis thaliana (thale cress)
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
            Camelineae; Arabidopsis.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Tabata,S., Kaneko,T., Nakamura,Y., Kotani,H., Kato,T., Asamizu,E.,
            Miyajima,N., Sasamoto,S., Kimura,T., Hosouchi,T., Kawashima,K.,
            Kohara,M., Matsumoto,M., Matsuno,A., Muraki,A., Nakayama,S.,
            Nakazaki,N., Naruo,K., Okumura,S., Shinpo,S., Takeuchi,C., Wada,T.,
            Watanabe,A., Yamada,M., Yasuda,M., Sato,S., de la Bastide,M.,
            Huang,E., Spiegel,L., Gnoj,L., O'Shaughnessy,A., Preston,R.,
            Habermann,K., Murray,J., Johnson,D., Rohlfing,T., Nelson,J.,
            Stoneking,T., Pepin,K., Spieth,J., Sekhon,M., Armstrong,J.,
            Becker,M., Belter,E., Cordum,H., Cordes,M., Courtney,L.,
            Courtney,W., Dante,M., Du,H., Edwards,J., Fryman,J., Haakensen,B.,
            Lamar,E., Latreille,P., Leonard,S., Meyer,R., Mulvaney,E.,
            Ozersky,P., Riley,A., Strowmatt,C., Wagner-McPherson,C., Wollam,A.,
            Yoakum,M., Bell,M., Dedhia,N., Parnell,L., Shah,R., Rodriguez,M.,
            See,L.H., Vil,D., Baker,J., Kirchoff,K., Toth,K., King,L.,
            Bahret,A., Miller,B., Marra,M., Martienssen,R., McCombie,W.R.,
            Wilson,R.K., Murphy,G., Bancroft,I., Volckaert,G., Wambutt,R.,
            Dusterhoft,A., Stiekema,W., Pohl,T., Entian,K.D., Terryn,N.,
            Hartley,N., Bent,E., Johnson,S., Langham,S.A., McCullagh,B.,
            Robben,J., Grymonprez,B., Zimmermann,W., Ramsperger,U., Wedler,H.,
            Balke,K., Wedler,E., Peters,S., van Staveren,M., Dirkse,W.,
            Mooijman,P., Lankhorst,R.K., Weitzenegger,T., Bothe,G., Rose,M.,
            Hauf,J., Berneiser,S., Hempel,S., Feldpausch,M., Lamberth,S.,
            Villarroel,R., Gielen,J., Ardiles,W., Bents,O., Lemcke,K.,
            Kolesov,G., Mayer,K., Rudd,S., Schoof,H., Schueller,C.,
            Zaccaria,P., Mewes,H.W., Bevan,M. and Fransz,P.
  CONSRTM   Kazusa DNA Research Institute; Cold Spring Harbor and Washington
            University in St Louis Sequencing Consortium; European Union
            Arabidopsis Genome Sequencing Consortium
  TITLE     Sequence and analysis of chromosome 5 of the plant Arabidopsis
            thaliana
  JOURNAL   Nature 408 (6814), 823-826 (2000)
   PUBMED   11130714
REFERENCE   2  (bases 1 to 3186)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 3186)
  AUTHORS   Krishnakumar,V., Cheng,C.-Y., Chan,A.P., Schobel,S., Kim,M.,
            Ferlanti,E.S., Belyaeva,I., Rosen,B.D., Micklem,G., Miller,J.R.,
            Vaughn,M. and Town,C.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Plant Genomics, J. Craig Venter Institute,
            9704 Medical Center Dr, Rockville, MD 20850, USA
  REMARK    Protein update by submitter
REFERENCE   4  (bases 1 to 3186)
  AUTHORS   Swarbreck,D., Lamesch,P., Wilks,C. and Huala,E.
  CONSRTM   TAIR
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2011) Department of Plant Biology, Carnegie
            Institution, 260 Panama Street, Stanford, CA, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by TAIR and Araport.
            This record is derived from an annotated genomic sequence
            (NC_003076).
            
            On Apr 18, 2007 this sequence version replaced NM_121549.2.
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Arabidopsis thaliana"
                     /mol_type="mRNA"
                     /db_xref="taxon:3702"
                     /chromosome="5"
                     /ecotype="Columbia"
     gene            1..3186
                     /gene="CLPB3"
                     /locus_tag="AT5G15450"
                     /gene_synonym="ALBINO AND PALE GREEN 6; APG6; AtCLPB3;
                     CASEIN LYTIC PROTEINASE B-P; casein lytic proteinase B3;
                     CLPB-P; T20K14.60; T20K14_60"
                     /note="Encodes a chloroplast-targeted Hsp101 homologue.
                     Functions as a molecular chaperone involved in plastid
                     differentiation mediating internal thylakoid membrane
                     formation and conferring thermotolerance to chloroplasts
                     during heat stress. APG6 is constitutively expressed in
                     the root tips, the organ boundary region, the reproductive
                     tissues of mature plants where plastids exist as
                     proplastids, and slightly in the stems and leaves. APG6
                     expression is upregulated in response to heat shock in
                     various organs, but not in response to other abiotic
                     stresses. Apg6 mutants have a pale-green phenotype."
                     /db_xref="Araport:AT5G15450"
                     /db_xref="GeneID:831398"
                     /db_xref="TAIR:AT5G15450"
     CDS             93..2999
                     /gene="CLPB3"
                     /locus_tag="AT5G15450"
                     /gene_synonym="ALBINO AND PALE GREEN 6; APG6; AtCLPB3;
                     CASEIN LYTIC PROTEINASE B-P; casein lytic proteinase B3;
                     CLPB-P; T20K14.60; T20K14_60"
                     /inference="Similar to RNA sequence,
                     EST:INSD:EL131988.1,INSD:EL030402.1,INSD:DR379872.1,
                     INSD:EG511692.1,INSD:ES132850.1,INSD:EH815343.1,
                     INSD:EL044628.1,INSD:EL112539.1,INSD:ES110440.1,
                     INSD:EL150642.1,INSD:EL998130.1,INSD:DR375532.1,
                     INSD:EL265955.1,INSD:EL020516.1,INSD:BP608262.1,
                     INSD:AV801744.1,INSD:EL280384.1,INSD:ES130915.1,
                     INSD:AV565466.1,INSD:EH954313.1,INSD:EL109529.1,
                     INSD:ES165540.1,INSD:EH907801.1,INSD:ES119836.1,
                     INSD:BP616071.1,INSD:EL197864.1,INSD:EL105873.1,
                     INSD:CA781786.1,INSD:ES089795.1,INSD:EL143713.1,
                     INSD:ES147910.1,INSD:AI996917.1,INSD:EL143912.1,
                     INSD:EH959729.1,INSD:EL089032.1,INSD:ES071406.1,
                     INSD:BP669177.1,INSD:EL279488.1,INSD:EL290958.1,
                     INSD:EL998807.1,INSD:AV792406.1,INSD:ES216287.1,
                     INSD:EL256313.1,INSD:EL295464.1,INSD:EL137035.1,
                     INSD:EL115360.1,INSD:ES119398.1,INSD:EL055455.1,
                     INSD:ES054050.1,INSD:ES062955.1,INSD:EG511691.1"
                     /inference="similar to RNA sequence,
                     mRNA:INSD:BT002569.1,INSD:BT000447.1"
                     /note="casein lytic proteinase B3 (CLPB3); FUNCTIONS IN:
                     nucleoside-triphosphatase activity, ATPase activity,
                     nucleotide binding, ATP binding; INVOLVED IN: chloroplast
                     organization, response to heat; LOCATED IN: plastid
                     stroma, chloroplast, chloroplast stroma; EXPRESSED IN: 28
                     plant structures; EXPRESSED DURING: 14 growth stages;
                     CONTAINS InterPro DOMAIN/s: Clp ATPase, C-terminal
                     (InterPro:IPR019489), ATPase, AAA+ type, core
                     (InterPro:IPR003593), ATPase, AAA-2 (InterPro:IPR013093),
                     ATPase, AAA-type, core (InterPro:IPR003959), Chaperonin
                     clpA/B (InterPro:IPR001270), Chaperonin ClpB
                     (InterPro:IPR017730), Chaperonin ClpA/B, conserved site
                     (InterPro:IPR018368), Clp, N-terminal
                     (InterPro:IPR004176); BEST Arabidopsis thaliana protein
                     match is: casein lytic proteinase B4 (TAIR:AT2G25140.1);
                     Has 30201 Blast hits to 17322 proteins in 780 species:
                     Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi -
                     3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996
                     (source: NCBI BLink)."
                     /codon_start=1
                     /product="casein lytic proteinase B3"
                     /protein_id="NP_568314.1"
                     /db_xref="GeneID:831398"
                     /db_xref="TAIR:AT5G15450"
                     /db_xref="Araport:AT5G15450"
                     /translation="

MATATTTATAAFSGVVSVGTETRRIYSFSHLQPSAAFPAKPSSFKSLKLKQSARLTRRLDHRPFVVRCEASSSNGRLTQQEFTEMAWQSIVSSPDVAKENKQQIVETEHLMKALLEQKNGLARRIFSKIGVDNTKVLEATEKFIQRQPKVYGDAAGSMLGRDLEALFQRARQFKKDLKDSYVSVEHLVLAFADDKRFGKQLFKDFQISERSLKSAIESIRGKQSVIDQDPEGKYEALEKYGKDLTAMAREGKLDPVIGRDDEIRRCIQILSRRTKNNPVLIGEPGVGKTAISEGLAQRIVQGDVPQALMNRKLISLDMGALIAGAKYRGEFEDRLKAVLKEVTDSEGQIILFIDEIHTVVGAGATNGAMDAGNLLKPMLGRGELRCIGATTLDEYRKYIEKDPALERRFQQVYVDQPTVEDTISILRGLRERYELHHGVRISDSALVEAAILSDRYISGRFLPDKAIDLVDEAAAKLKMEITSKPTALDELDRSVIKLEMERLSLTNDTDKASRERLNRIETELVLLKEKQAELTEQWEHERSVMSRLQSIKEEIDRVNLEIQQAEREYDLNRAAELKYGSLNSLQRQLNEAEKELNEYLSSGKSMFREEVLGSDIAEIVSKWTGIPVSKLQQSERDKLLHLEEELHKRVVGQNPAVTAVAEAIQRSRAGLSDPGRPIASFMFMGPTGVGKTELAKALASYMFNTEEALVRIDMSEYMEKHAVSRLIGAPPGYVGYEEGGQLTETVRRRPYSVILFDEIEKAHGDVFNVFLQILDDGRVTDSQGRTVSFTNTVIIMTSNVGSQFILNNTDDDANELSYETIKERVMNAARSIFRPEFMNRVDEYIVFKPLDREQINRIVRLQLARVQKRIADRKMKINITDAAVDLLGSLGYDPNYGARPVKRVIQQNIENELAKGILRGDFKEEDGILIDTEVTAFSNGQLPQQKLTFKKIESETADAEQEEAAFSK"

     misc_feature    336..2891
                     /gene="CLPB3"
                     /locus_tag="AT5G15450"
                     /gene_synonym="ALBINO AND PALE GREEN 6; APG6; AtCLPB3;
                     CASEIN LYTIC PROTEINASE B-P; casein lytic proteinase B3;
                     CLPB-P; T20K14.60; T20K14_60"
                     /note="ATP-dependent chaperone ClpB; Region:
                     chaperone_ClpB; TIGR03346"
                     /db_xref="CDD:274529"
ORIGIN      

acatctcgccgttttggtggatttgtgctaaactacctgttgtatttgatcttcctttttcaattgcttgctgttcttaaatttccgagctaatggcgacggctacgacgactgctacggcggcgtttagtggtgtagtcagtgtaggaacggagactcgaaggatttattcgttttctcatcttcaaccttctgcggcttttccggcgaagcctagttccttcaaatctctcaaattaaagcagagcgcgaggctcacacggcggcttgatcatcggccgttcgttgtccgatgtgaagcttcttcttctaatggaaggcttacacagcaagaattcacagaaatggcgtggcaatcgatagtttcgtcaccagatgttgcaaaagagaataagcaacagattgtggagacagagcatctaatgaaagctcttttggagcagaagaatggtttagctcgtaggattttctctaagatcggtgtggataacacaaaggttctagaagcaacagagaagttcattcaacgccaaccaaaggtgtatggagatgctgctggttcgatgttggggcgtgatttggaagctctgttccagagagcaagacagtttaagaaagatttaaaggactcttatgtgtcagtggagcatttggttcttgcttttgctgatgataagcggtttgggaaacagttgtttaaggattttcagatatcagagaggagcttgaaatctgcaattgaatccattagagggaaacaatcagtcattgaccaagatccggaaggcaagtatgaggcgttggagaagtatggaaaagacttgacagcaatggcaagagagggaaaactagatcctgtgataggtagagatgacgagatccgaagatgcattcagattctctcaaggagaacaaagaacaaccctgtgttgatcggtgaaccaggtgttggtaaaactgcaatttcagaaggacttgctcagaggattgtgcaaggagatgtacctcaagcattgatgaacagaaagttgatatctcttgatatgggtgctctcattgctggagcaaagtatcggggagaattcgaagacagactaaaagctgtgcttaaggaagtcacagactcagaaggccaaatcattttgttcatcgatgagatccatacagttgttggtgcaggtgcgactaatggggccatggatgctggtaatctgctgaaaccgatgctgggccgaggcgagttacgttgtatcggtgcaaccacgcttgatgaatataggaaatacatagagaaagatccagccttggagcgtaggtttcaacaggtttatgttgatcaacctacggttgaagacacgatttctattctccgtggtttgcgggaaaggtatgaactacatcatggagttcgcatctcagatagtgctctagtggaagctgctatcctctcagaccgttacatcagtggccggtttctacccgataaagcaattgacttggtcgatgaagcggcagccaaattgaaaatggaaatcacttcgaaacctacagctcttgacgagcttgaccgttcggtaataaagcttgaaatggagaggctttcacttaccaatgatacagacaaagcttctagagagagattgaatcgaattgaaactgagttagtgctattaaaggagaaacaagctgagttaactgaacagtgggagcacgagaggtctgttatgtcccgtcttcagtcgattaaagaggagattgatcgagtaaacctcgagatccagcaggcggaacgtgaatacgatctcaaccgtgcagctgagctcaaatacggaagcttaaactcattgcagcgacagcttaatgaagcagagaaagagctgaacgagtacttaagctctgggaaatctatgttcagagaagaggttttgggtagcgatatcgccgagattgtgagtaaatggactggaatcccggtatcaaaacttcaacagtctgaacgagataagcttctacatttggaagaagaattgcataaaagagtagtcggtcaaaacccagcagtgactgcggtagctgaagcaatccaacgatcaagagctggtctttcggatccaggccgtcctatagctagtttcatgtttatgggaccaactggtgttggtaaaacagagctagccaaagcacttgcttcttatatgttcaacactgaagaagctttagtgagaattgacatgagtgaatacatggagaagcatgcggtgtctaggctcattggagctccacctggatatgttggttacgaagaaggaggacagttaacagaaacggttagaagaaggccttattcggtcattctatttgatgagattgagaaagctcatggagatgtcttcaatgtgtttctccaaatcttggacgatgggcgagtcacagattctcaaggacgtacagtgagtttcaccaataccgtcattattatgacctcaaatgttggttcgcagtttattctgaacaacacagatgatgatgctaatgagctctcttatgaaacgattaaagagagagtgatgaatgctgcaaggtcaatctttcgtcctgaattcatgaatagggttgatgagtatatagttttcaaacctcttgaccgcgagcagatcaaccgcattgtccggttacagctggcacgtgtgcagaagcgaattgcagacaggaaaatgaagataaacatcacggatgcagcagtcgatcttcttggaagcctagggtatgatccaaactatggagctagacctgttaagcgtgtgatacagcaaaacattgagaatgagcttgctaagggtatcttgagaggagatttcaaggaggaagatggcattttgatcgataccgaggtcacagcgttctccaatggccagttacctcagcaaaagctcacattcaagaagattgagtcagaaacagcagatgctgaacaagaagaagcagctttctcaaaatagagtagttgaaaatgcaaccttttggagcccaagaagattcagacttttcagtacagaacttctagtgagttacttgtcacaaaagactgataatatttatctcaatgtgagagtttatataaatcataagcttatgagtcattgtactgatgtctcaaaggtccagaggttatgaaaattgttgatc

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