LOCUS       NM_001368357            2104 bp    mRNA    linear   ROD 17-JUN-2024
DEFINITION  Mus musculus double C2, alpha (Doc2a), transcript variant 4, mRNA.
ACCESSION   NM_001368357 XM_017321967
VERSION     NM_001368357.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Guiberson,N.G.L., Black,L.S., Haller,J.E., Brukner,A., Abramov,D.,
            Ahmad,S., Xie,Y.X., Sharma,M. and Burre,J.
  TITLE     Disease-linked mutations in Munc18-1 deplete synaptic Doc2
  JOURNAL   Brain 147 (6), 2185-2202 (2024)
   PUBMED   38242640
  REMARK    GeneRIF: Disease-linked mutations in Munc18-1 deplete synaptic
            Doc2.
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Wu,Z., Kusick,G.F., Berns,M.M.M., Raychaudhuri,S., Itoh,K.,
            Walter,A.M., Chapman,E.R. and Watanabe,S.
  TITLE     Synaptotagmin 7 docks synaptic vesicles to support facilitation and
            Doc2alpha-triggered asynchronous release
  JOURNAL   Elife 12, 90632 (2024)
   PUBMED   38536730
  REMARK    GeneRIF: Synaptotagmin 7 docks synaptic vesicles to support
            facilitation and Doc2alpha-triggered asynchronous release.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2104)
  AUTHORS   Wang,Q.W., Qin,J., Chen,Y.F., Tu,Y., Xing,Y.Y., Wang,Y., Yang,L.Y.,
            Lu,S.Y., Geng,L., Shi,W., Yang,Y. and Yao,J.
  TITLE     16p11.2 CNV gene Doc2alpha functions in neurodevelopment and social
            behaviors through interaction with Secretagogin
  JOURNAL   Cell Rep 42 (7), 112691 (2023)
   PUBMED   37354460
  REMARK    GeneRIF: 16p11.2 CNV gene Doc2alpha functions in neurodevelopment
            and social behaviors through interaction with Secretagogin.
REFERENCE   4  (bases 1 to 2104)
  AUTHORS   Bourgeois-Jaarsma,Q., Miaja Hernandez,P. and Groffen,A.J.
  TITLE     Ca2+ sensor proteins in spontaneous release and synaptic
            plasticity: Limited contribution of Doc2c, rabphilin-3a and
            synaptotagmin 7 in hippocampal glutamatergic neurons
  JOURNAL   Mol Cell Neurosci 112, 103613 (2021)
   PUBMED   33753311
  REMARK    GeneRIF: Ca(2+) sensor proteins in spontaneous release and synaptic
            plasticity: Limited contribution of Doc2c, rabphilin-3a and
            synaptotagmin 7 in hippocampal glutamatergic neurons.
REFERENCE   5  (bases 1 to 2104)
  AUTHORS   Diez-Arazola,R., Meijer,M., Bourgeois-Jaarsma,Q., Cornelisse,L.N.,
            Verhage,M. and Groffen,A.J.
  TITLE     Doc2 Proteins Are Not Required for the Increased Spontaneous
            Release Rate in Synaptotagmin-1-Deficient Neurons
  JOURNAL   J Neurosci 40 (13), 2606-2617 (2020)
   PUBMED   32098902
  REMARK    GeneRIF: Doc2 Proteins Are Not Required for the Increased
            Spontaneous Release Rate in Synaptotagmin-1-Deficient Neurons.
REFERENCE   6  (bases 1 to 2104)
  AUTHORS   Groffen,A.J., Friedrich,R., Brian,E.C., Ashery,U. and Verhage,M.
  TITLE     DOC2A and DOC2B are sensors for neuronal activity with unique
            calcium-dependent and kinetic properties
  JOURNAL   J Neurochem 97 (3), 818-833 (2006)
   PUBMED   16515538
REFERENCE   7  (bases 1 to 2104)
  AUTHORS   Toonen,R.F., de Vries,K.J., Zalm,R., Sudhof,T.C. and Verhage,M.
  TITLE     Munc18-1 stabilizes syntaxin 1, but is not essential for syntaxin 1
            targeting and SNARE complex formation
  JOURNAL   J Neurochem 93 (6), 1393-1400 (2005)
   PUBMED   15935055
REFERENCE   8  (bases 1 to 2104)
  AUTHORS   Bouwman,J., Maia,A.S., Camoletto,P.G., Posthuma,G., Roubos,E.W.,
            Oorschot,V.M., Klumperman,J. and Verhage,M.
  TITLE     Quantification of synapse formation and maintenance in vivo in the
            absence of synaptic release
  JOURNAL   Neuroscience 126 (1), 115-126 (2004)
   PUBMED   15145078
REFERENCE   9  (bases 1 to 2104)
  AUTHORS   Sakaguchi,G., Manabe,T., Kobayashi,K., Orita,S., Sasaki,T.,
            Naito,A., Maeda,M., Igarashi,H., Katsuura,G., Nishioka,H.,
            Mizoguchi,A., Itohara,S., Takahashi,T. and Takai,Y.
  TITLE     Doc2alpha is an activity-dependent modulator of excitatory synaptic
            transmission
  JOURNAL   Eur J Neurosci 11 (12), 4262-4268 (1999)
   PUBMED   10594652
REFERENCE   10 (bases 1 to 2104)
  AUTHORS   Naito,A., Orita,S., Wanaka,A., Sasaki,T., Sakaguchi,G., Maeda,M.,
            Igarashi,H., Tohyama,M. and Takai,Y.
  TITLE     Molecular cloning of mouse Doc2alpha and distribution of its mRNA
            in adult mouse brain
  JOURNAL   Brain Res Mol Brain Res 44 (2), 198-204 (1997)
   PUBMED   9073161
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124505.4.
            
            On Feb 8, 2019 this sequence version replaced XM_017321967.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.4256118.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                AC124505.4         188485-188530
            47-494              AC124505.4         189183-189630
            495-574             AC124505.4         189988-190067
            575-649             AC124505.4         190250-190324
            650-759             AC124505.4         190430-190539
            760-886             AC124505.4         191702-191828
            887-946             AC124505.4         191908-191967
            947-1110            AC124505.4         192051-192214
            1111-1192           AC124505.4         192305-192386
            1193-1289           AC124505.4         192477-192573
            1290-2104           AC124505.4         192659-193473
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 69.25 cM"
     gene            1..2104
                     /gene="Doc2a"
                     /note="double C2, alpha"
                     /db_xref="GeneID:13446"
                     /db_xref="MGI:MGI:109446"
     exon            1..46
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            47..494
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    152..154
                     /gene="Doc2a"
                     /note="upstream in-frame stop codon"
     CDS             218..1435
                     /gene="Doc2a"
                     /note="doc2-alpha"
                     /codon_start=1
                     /product="double C2-like domain-containing protein alpha"
                     /protein_id="NP_001355286.1"
                     /db_xref="CCDS:CCDS21847.1"
                     /db_xref="GeneID:13446"
                     /db_xref="MGI:MGI:109446"
                     /translation="

MRGRRGDRMTINIQEHMAINVCPGPIRPIRQISDYFPRRGPGPEGGGGGGGTGCGEAPAHLAPLALAPPAALLGATTPDDGAEVDSYDSDDTTALGTLEFDLLYDQASCMLHCRILRAKGLKPMDFNGLADPYVKLHLLPGACKANKLKTKTQRNTLNPVWNEELTYSGITDDDITHKVLRISVCDEDKLSHNEFIGEIRVPLRRLKPSQKKHFNICLERQVPLPSPSSMSAALRGISCYLKELEQAEQGPGLLEERGRILLSLSYSSRRHGLLVGIVRCAHLAAMDVNGYSDPYVKTYLRPDVDKKSKHKTCVKKKTLNPEFNEEFFYEIELSTLATKTLEVTVWDYDIGKSNDFIGGVSLGPGARGEAQKHWNDCLHQPDTALERWHTLTSELPPAAGAYPLA"

     misc_feature    218..499
                     /gene="Doc2a"
                     /note="propagated from UniProtKB/Swiss-Prot (Q7TNF0.1);
                     Region: Interaction with UNC13D and DYNLT1.
                     /evidence=ECO:0000250"
     misc_feature    317..379
                     /gene="Doc2a"
                     /note="propagated from UniProtKB/Swiss-Prot (Q7TNF0.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    500..871
                     /gene="Doc2a"
                     /note="C2 domain first repeat present in Rabphilin and
                     Double C2 domain; Region: C2A_Rabphilin_Doc2; cd04035"
                     /db_xref="CDD:176000"
     misc_feature    order(590..592,608..610,773..775,779..781,797..799)
                     /gene="Doc2a"
                     /note="Ca2+ binding site [ion binding]; other site"
                     /db_xref="CDD:176000"
     misc_feature    875..1432
                     /gene="Doc2a"
                     /note="propagated from UniProtKB/Swiss-Prot (Q7TNF0.1);
                     Region: Interaction with UNC13D. /evidence=ECO:0000250"
     misc_feature    992..1390
                     /gene="Doc2a"
                     /note="C2 domain second repeat present in Rabphilin and
                     Double C2 domain; Region: C2B_Rabphilin_Doc2; cd08384"
                     /db_xref="CDD:176030"
     misc_feature    order(1076..1078,1094..1096,1256..1258,1262..1264,
                     1280..1282)
                     /gene="Doc2a"
                     /note="Ca2+ binding site [ion binding]; other site"
                     /db_xref="CDD:176030"
     exon            495..574
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            575..649
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            650..759
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            760..886
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            887..946
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1110
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1192
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1289
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..2104
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2085..2090
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Doc2a"
                     /note="hexamer: AATAAA"
     polyA_site      2104
                     /gene="Doc2a"
                     /note="major polyA site"
ORIGIN      

ggggaacaccgggcgcctctcgcggaggtgcacgccaagttctcgggactgatctggcaacccacccagccctttgtgaagccaggcctcctgcctgcccaccagccagcgcagatcatcttttccctcgacacccaggaagggagggcagtgaggttcctacagaccccagccggccactccagctctacacccgtctcccagtcagaggtgctgcatgaggggccgcaggggcgatcgcatgaccatcaacatccaggagcacatggccatcaacgtgtgccctggacccatcaggcccatccgccagatctccgactacttccctcgcagggggccaggaccagagggtggcggcggcggcggtggcacgggctgcggggaagccccagctcatctggcccctctggctctggccccccctgcggctctccttggggccactacacccgacgatggagctgaggtagacagctacgactcggatgataccaccgccctgggcacactggaatttgaccttctctatgatcaggcttcctgcatgctgcactgtagaatcctcagggccaagggcctcaagcccatggatttcaatggcctggctgacccctatgtaaagcttcacctcctgccaggagcctgcaaggccaataagctaaaaaccaagacacagaggaacacactgaaccctgtgtggaatgaggagctgacgtacagcgggatcacggatgatgacatcacccacaaggtgctcaggatctctgtctgtgatgaggacaagctgagccacaatgaattcattggggagatccgagtgcccctccgccgcctcaagccttcacagaagaagcattttaacatctgccttgagcgccaggtcccgcttccttcaccctcttcaatgtctgcggcgctgaggggcatatcctgttacctgaaggagctggagcaggcagagcagggacctgggctgctggaagagcgcgggcgcatcctgctgagcctcagctacagctctcggcggcatgggctgctggtgggcattgttcgctgtgcgcacctggctgcaatggatgttaacggctactctgacccttatgtgaagacgtacttgagaccagatgtggataagaaatccaagcacaaaacatgtgtaaagaagaagacactaaatccggaatttaatgaggaattcttctatgagattgaactctccactctggccactaagaccctggaggtcacagtctgggactacgacattggcaaatccaatgacttcataggtggtgtgtctctggggccaggagcccggggagaggcccagaaacactggaatgactgtctacatcagccggacacagccctggagcgctggcatactctgaccagcgagctgccccctgcagcaggggcttaccctttggcttgagtgaacagcagctgtccatcacaggccctctggggccgggtccagtacccaaccttcgcacgagtgtgttgcacgtttacatggggtgggatgcccctgccccacactacctgtcttatttttgtgagtctctgtgacgggcctgtcttcttgtgagggactgtggagagctatattcacatatgcaaacttcctcctgcctgccttgctgttacctgcaaatatgcaaccacccgtgcacagggccacgtgggagtctcctgccagtgccccaccccatcctgcagctcctttcttggcctttccaggctgcctggtcccctgccccacagggagggggtcggattagggaagattagaggaggacgtttccaaatagaggaaaggacaaggaaagaaagagccaactctttaatacagagccttcactaaatcccagccctgcgtagctgctcttctaaaggggcggccaccgtaggtctctacctcccgaagatgtagcagacccttttggccactcgtttaaataactgttccctggatggggctgggatgtaagggcaggaccctgccaccttcctcggggacattgtgcatgtttacgccttttctgattgtgtcagtggccgaagcatggcaactgggcatcaataaacatctcttgaggaa

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