LOCUS       NM_033612               1124 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus chymotrypsin-like elastase family, member 1 (Cela1),
            mRNA.
ACCESSION   NM_033612 XM_001004573 XM_001004575 XM_001004577
VERSION     NM_033612.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Ojha,M., Smith,N.J., Devine,A.J., Joshi,R., Goodman,E.M., Fan,Q.,
            Schuman,R., Porollo,A., Wells,J.M., Tiwary,E., Batie,M.R., Gray,J.,
            Deshmukh,H., Borchers,M.T., Ammerman,S.A. and Varisco,B.M.
  TITLE     Anti-CELA1 antibody KF4 prevents emphysema by inhibiting
            stretch-mediated remodeling
  JOURNAL   JCI Insight 9 (1), e169189 (2024)
   PUBMED   38193533
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1124)
  AUTHORS   Cobo,I., Paliwal,S., Bodas,C., Felipe,I., Melia-Aloma,J.,
            Torres,A., Martinez-Villarreal,J., Malumbres,M., Garcia,F.,
            Millan,I., Del Pozo,N., Park,J.C., MacDonald,R.J., Munoz,J.,
            Mendez,R. and Real,F.X.
  TITLE     NFIC regulates ribosomal biology and ER stress in pancreatic acinar
            cells and restrains PDAC initiation
  JOURNAL   Nat Commun 14 (1), 3761 (2023)
   PUBMED   37353485
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1124)
  AUTHORS   Zhao,H., Huang,X., Liu,Z., Lai,L., Sun,R., Shen,R., Li,Y., He,L.,
            Pu,W., Lv,Z., Li,Y., Han,X., Liu,X. and Zhou,B.
  TITLE     Use of a dual genetic system to decipher exocrine cell fate
            conversions in the adult pancreas
  JOURNAL   Cell Discov 9 (1), 1 (2023)
   PUBMED   36596774
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1124)
  AUTHORS   Martinez-Ramirez,A.S., Borders,T.L., Paul,L., Schipma,M., Wang,X.,
            Korobova,F., Wright,C.V. and Sosa-Pineda,B.
  TITLE     Specific Temporal Requirement of Prox1 Activity During Pancreatic
            Acinar Cell Development
  JOURNAL   Gastro Hep Adv 1 (5), 807-823 (2022)
   PUBMED   37829188
REFERENCE   6  (bases 1 to 1124)
  AUTHORS   Hameister,H., Schulz,W.A., Meyer,J., Thoma,S., Adolph,S., Gaa,A.
            and von Deimling,O.
  TITLE     Gene order and genetic distance of 13 loci spanning murine
            chromosome 15
  JOURNAL   Genomics 14 (2), 417-422 (1992)
   PUBMED   1358803
REFERENCE   7  (bases 1 to 1124)
  AUTHORS   Brannan,C.I., Gilbert,D.J., Ceci,J.D., Matsuda,Y., Chapman,V.M.,
            Mercer,J.A., Eisen,H., Johnston,L.A., Copeland,N.G. and
            Jenkins,N.A.
  TITLE     An interspecific linkage map of mouse chromosome 15 positioned with
            respect to the centromere
  JOURNAL   Genomics 13 (4), 1075-1081 (1992)
   PUBMED   1354638
REFERENCE   8  (bases 1 to 1124)
  AUTHORS   Serikawa,T., Kuramoto,T., Hilbert,P., Mori,M., Yamada,J.,
            Dubay,C.J., Lindpainter,K., Ganten,D., Guenet,J.L., Lathrop,G.M. et
            al.
  TITLE     Rat gene mapping using PCR-analyzed microsatellites
  JOURNAL   Genetics 131 (3), 701-721 (1992)
   PUBMED   1628813
REFERENCE   9  (bases 1 to 1124)
  AUTHORS   Yamasaki,N., Sugimura,K., Hiida,M., Naito,T. and Watanabe,T.
  TITLE     Sequence analysis of a cDNA clone of a gene encoding a component of
            a putative phosphorylcholine-specific T suppressor factor and
            functional property of its gene product
  JOURNAL   Eur J Immunol 17 (2), 247-253 (1987)
   PUBMED   3493908
REFERENCE   10 (bases 1 to 1124)
  AUTHORS   Honey,N.K., Sakaguchi,A.Y., Lalley,P.A., Quinto,C., MacDonald,R.J.,
            Craik,C., Bell,G.I., Rutter,W.J. and Naylor,S.L.
  TITLE     Chromosomal assignments of genes for trypsin, chymotrypsin B, and
            elastase in mouse
  JOURNAL   Somat Cell Mol Genet 10 (4), 377-383 (1984)
   PUBMED   6589791
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB951068.1, AK007392.1 and BP769372.1.
            
            On Jan 18, 2008 this sequence version replaced NM_033612.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK007392.1, BC011218.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849388
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-628               CB951068.1         1-628
            629-1077            AK007392.1         627-1075
            1078-1124           BP769372.1         1-47                c
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 56.38 cM"
     gene            1..1124
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /note="chymotrypsin-like elastase family, member 1"
                     /db_xref="GeneID:109901"
                     /db_xref="MGI:MGI:95314"
     exon            1..67
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..828
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /EC_number="3.4.21.36"
                     /note="elastase 1, pancreatic; elastase-1"
                     /codon_start=1
                     /product="chymotrypsin-like elastase family member 1
                     precursor"
                     /protein_id="NP_291090.2"
                     /db_xref="CCDS:CCDS37213.1"
                     /db_xref="GeneID:109901"
                     /db_xref="MGI:MGI:95314"
                     /translation="

MLRFLVFASLVLCGHSTEDVPETDARVVGGAEARRNSWPSQISLQYQYGGSWHHTCGGTLIRSNWVMTAAHCVDSPMTYRVVVGEHNLSQNDGTEQYVNVQKIVSHPYWNKNNVVAGYDIALLRLAKSVTLNNYVQLGVLPREGTILANNSPCYITGWGRTRTNGELAQTLQQAYLPSVSYSICSSSSYWGSSVKNTMVCAGGDGVRSGCQGDSGGPLHCMVNGQYAVHGVTSFVSSMGCNVARKPTVFTRVSAYISWMNNVIASN"

     sig_peptide     28..75
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
     mat_peptide     106..825
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /product="Chymotrypsin-like elastase family member 1.
                     /id=PRO_0000416104"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91X79.1)"
     misc_feature    106..813
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...; Region:
                     Tryp_SPc; cd00190"
                     /db_xref="CDD:238113"
     misc_feature    106..108
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     misc_feature    order(238..240,382..384,667..669)
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /note="active site"
                     /db_xref="CDD:238113"
     misc_feature    286..288
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q91X79.1); glycosylation site"
     misc_feature    order(649..651,724..726,730..732)
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /note="substrate binding sites [chemical binding]; other
                     site"
                     /db_xref="CDD:238113"
     exon            68..150
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /inference="alignment:Splign:2.1.0"
     exon            151..251
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /inference="alignment:Splign:2.1.0"
     exon            252..377
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /inference="alignment:Splign:2.1.0"
     exon            378..514
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /inference="alignment:Splign:2.1.0"
     exon            515..660
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /inference="alignment:Splign:2.1.0"
     exon            661..810
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /inference="alignment:Splign:2.1.0"
     exon            811..1106
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1083..1088
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /note="hexamer: AATAAA"
     polyA_site      1104
                     /gene="Cela1"
                     /gene_synonym="1810009A17Rik; 1810062B19Rik; Ela-1; Ela1;
                     PC-TsF"
                     /note="major polyA site"
ORIGIN      

gggacagtggtctactctctccacaacatgttgcgcttcctggtgtttgcttccctggtcctgtgtggacacagtaccgaggacgttccggaaactgacgcccgcgtggttggaggggctgaagcccggaggaactcttggccgtctcagatttccctccagtaccagtatggaggatcatggcaccacacctgtggaggcaccctcatccgaagcaactgggtgatgaccgctgctcactgtgtggacagccccatgacttatcgagtggttgtcggagagcacaacctgagccagaatgacggcaccgagcagtatgtgaacgtgcagaagattgtgtcacacccctactggaacaagaacaacgtggttgcaggctatgacatcgccctgctgcgcttggccaagagtgttacactcaataactatgtccagctgggtgttctgccccgggagggaaccatcctggctaacaactctccctgctacatcacaggctggggaagaaccagaaccaatggggagctggcccagaccctgcagcaggcatacctgcccagcgtgtcctatagcatctgctccagctcctcttactggggctcctctgtgaagaataccatggtgtgcgctggtggagatggagtccgctctggatgccagggtgattctgggggacccctccactgcatggtgaacggtcagtatgctgtccacggagtgaccagctttgtgtccagcatgggctgtaatgtcgccaggaagcccaccgtcttcaccagagtctctgcttacatttcctggatgaataatgtcattgcctccaactgagcgacttccggagtccagtggcctccccaagatggctcttagctttgcaatgagacctgaagtaagcaagaaggagttagagctgaggttatcgctcagtggccgagcacttgtctaatatgtgcacagccttgggctccatccctaatactgcaacaggagcaggggaatgctgctggtgtcttggtatctggggcaaaggtggggggttaatgaaaagcaactcagactactgaatcagatacagaaaggcaaataaaaatcaatgtgttaccataaaaaaaaaaaaaaaaaaa

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