LOCUS       NM_008250               2171 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus H2.0-like homeobox (Hlx), mRNA.
ACCESSION   NM_008250
VERSION     NM_008250.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Zhang,H., Madi,A., Yosef,N., Chihara,N., Awasthi,A., Pot,C.,
            Lambden,C., Srivastava,A., Burkett,P.R., Nyman,J., Christian,E.,
            Etminan,Y., Lee,A., Stroh,H., Xia,J., Karwacz,K., Thakore,P.I.,
            Acharya,N., Schnell,A., Wang,C., Apetoh,L., Rozenblatt-Rosen,O.,
            Anderson,A.C., Regev,A. and Kuchroo,V.K.
  TITLE     An IL-27-Driven Transcriptional Network Identifies Regulators of
            IL-10 Expression across T Helper Cell Subsets
  JOURNAL   Cell Rep 33 (8), 108433 (2020)
   PUBMED   33238123
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Farrell,S.A., Sodhi,S., Marshall,C.R., Guerin,A., Slavotinek,A.,
            Paton,T., Chong,K., Sirkin,W.L., Scherer,S.W., Berube-Simard,F.A.
            and Pilon,N.
  TITLE     HLX is a candidate gene for a pattern of anomalies associated with
            congenital diaphragmatic hernia, short bowel, and asplenia
  JOURNAL   Am J Med Genet A 173 (11), 3070-3074 (2017)
   PUBMED   28898547
  REMARK    GeneRIF: HLX is a homeobox transcription factor gene which is
            relatively conserved across species. Hlx homozygous null mice have
            a short bowel and reduced muscle cells in the diaphragm, closely
            resembling the anomalies in the two fetuses and we therefore
            suggest that the HLX mutation in this family could explain the
            fetal findings
REFERENCE   3  (bases 1 to 2171)
  AUTHORS   Huang,L., Pan,D., Chen,Q., Zhu,L.J., Ou,J., Wabitsch,M. and
            Wang,Y.X.
  TITLE     Transcription factor Hlx controls a systematic switch from white to
            brown fat through Prdm16-mediated co-activation
  JOURNAL   Nat Commun 8 (1), 68 (2017)
   PUBMED   28701693
  REMARK    GeneRIF: Prdm16 interacts with the transcription factor Hlx, which
            is stabilized in response to beta3-adrenergic signaling, to
            increase thermogenic gene expression and mitochondrial biogenesis
            in subcutaneous WAT.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2171)
  AUTHORS   Zhong,J., Yu,Q., Yang,P., Rao,X., He,L., Fang,J., Tu,Y., Zhang,Z.,
            Lai,Q., Zhang,S., Kuczma,M., Kraj,P., Xu,J.F., Gong,F., Zhou,J.,
            Wen,L., Eizirik,D.L., Du,J., Wang,W. and Wang,C.Y.
  TITLE     MBD2 regulates TH17 differentiation and experimental autoimmune
            encephalomyelitis by controlling the homeostasis of T-bet/Hlx axis
  JOURNAL   J Autoimmun 53, 95-104 (2014)
   PUBMED   24934598
  REMARK    GeneRIF: methyl-CpG-binding domain protein 2 (MBD2), an epigenetic
            regulator, controls autoimmunity and EAE through T-bet/Hlx.
REFERENCE   5  (bases 1 to 2171)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   6  (bases 1 to 2171)
  AUTHORS   Mullick,A., Trasler,D. and Gros,P.
  TITLE     High-resolution linkage map in the vicinity of the Lp locus
  JOURNAL   Genomics 26 (3), 479-488 (1995)
   PUBMED   7607671
REFERENCE   7  (bases 1 to 2171)
  AUTHORS   Engelman,R.W., Day,N.K. and Good,R.A.
  TITLE     Calorie intake during mammary development influences cancer risk:
            lasting inhibition of C3H/HeOu mammary tumorigenesis by
            peripubertal calorie restriction
  JOURNAL   Cancer Res 54 (21), 5724-5730 (1994)
   PUBMED   7923222
REFERENCE   8  (bases 1 to 2171)
  AUTHORS   Kennedy,M.A., Rayner,J.C. and Morris,C.M.
  TITLE     Genomic structure, promoter sequence, and revised translation of
            human homeobox gene HLX1
  JOURNAL   Genomics 22 (2), 348-355 (1994)
   PUBMED   7806220
REFERENCE   9  (bases 1 to 2171)
  AUTHORS   Murtha,M.T., Leckman,J.F. and Ruddle,F.H.
  TITLE     Detection of homeobox genes in development and evolution
  JOURNAL   Proc Natl Acad Sci U S A 88 (23), 10711-10715 (1991)
   PUBMED   1720547
REFERENCE   10 (bases 1 to 2171)
  AUTHORS   Allen,J.D., Lints,T., Jenkins,N.A., Copeland,N.G., Strasser,A.,
            Harvey,R.P. and Adams,J.M.
  TITLE     Novel murine homeo box gene on chromosome 1 expressed in specific
            hematopoietic lineages and during embryogenesis
  JOURNAL   Genes Dev 5 (4), 509-520 (1991)
   PUBMED   1672660
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC064068.1, AK036435.1, BB664380.1 and BC050146.1.
            
            On Apr 29, 2009 this sequence version replaced NM_008250.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC064068.1, AK036435.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-162               BC064068.1         1-162
            163-599             AK036435.1         124-560
            600-970             BB664380.1         224-594
            971-2096            BC050146.1         226-1351
            2097-2171           BC064068.1         2098-2172
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 88.97 cM"
     gene            1..2171
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /note="H2.0-like homeobox"
                     /db_xref="GeneID:15284"
                     /db_xref="MGI:MGI:96109"
     exon            1..935
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    56..58
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /note="upstream in-frame stop codon"
     CDS             353..1783
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /note="homeobox protein HLX1; H2.0-like homeo box 1"
                     /codon_start=1
                     /product="H2.0-like homeobox protein"
                     /protein_id="NP_032276.1"
                     /db_xref="CCDS:CCDS15593.1"
                     /db_xref="GeneID:15284"
                     /db_xref="MGI:MGI:96109"
                     /translation="

MFAAGLAPFYASNFSLWSAAYCSSAGPGGCSFALDPAAVKKPSFCIADILHAGVGEPGPAAEGLVGASAALTAHLGSVHPHASFQAAARSPLRPTPVVAPSEVPAGFPQRLSPLSAAFHQHLPQQSPTQQQQPQQQPPPPPRAVSLQPPTSGTRVVPHHSGSAPAPSSKDLKFGIDRILSAEFDPKVKEGNTLRDLTSLLTGGRPAGVHLAGLQPSAGQFFASLDPISEASAILSPLSSNPRNSVQHQFQDTFPGPYAVLTKDTMPQTYKRKRSWSRAVFSNLQRKGLEKRFEIQKYVTKPDRKQLAAMLGLTDAQVKVWFQNRRMKWRHSKEAQAQKDKDKEAGEKPSGGVPAEGEREERSPSRSEGEAESESSDSESLDMAPSDTERTEGTERSLHQTTVIKASAAGALITASSSTSGSSFSFSSTSSLGSGNTHVGSASSLGGNCSELPSAHQPSVTSSPQSPEIAQAPLAGL"

     misc_feature    710..859
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61670.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1160..1342
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /note="Region: Homeodomain; pfam00046"
                     /db_xref="CDD:459649"
     misc_feature    1334..1555
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61670.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1589..1780
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61670.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     exon            936..1115
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1300
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..2149
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2122..2127
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /note="hexamer: AATAAA"
     polyA_site      2149
                     /gene="Hlx"
                     /gene_synonym="Hlx1"
                     /note="major polyA site"
ORIGIN      

gctgtttgggtttttctttcggtgtcgggtccctgtctccccggcttctgcggagtagcagagcgaggccgcgctcgccttgtcgccccagcgcctggcgatcgcctccatccacccggcggggagcgcgcccgccgcgccgcctttcaagccaagccaggggcccaggccgtggcgggcggcgatcgggccctcgcctcgtccctcggtggcgctagggctccccggttggccctccgcttcagggcgggcgaagaaccgaaagcagctcgggctcggctgggacaccgcctctgcccgagcgcccacccacttccccactcacccaggaccccgccgggatcctgccaggatgttcgcagccggcctggctcccttctatgcttccaactttagtctctggtcagccgcctactgctcctcggcgggcccgggcggctgctccttcgccctggacccagccgctgtcaagaaaccctctttctgcatcgcggacatcctgcacgccggcgtcggggagccggggcccgctgcagagggactggtgggggcctcggcagccctcaccgcacacttgggctccgtgcacccgcacgcctccttccaagctgccgccagatccccgctccgtcccaccccggtggtggcgccctcagaagtcccggctggcttccctcagcggctgtctccgctctctgccgccttccaccagcatctcccgcagcaatcaccaacgcagcaacagcaaccgcagcagcagcccccgcctccaccccgggcggtctccctgcagccaccgacttcggggacgcgggtggtcccccaccatagcggctccgcccctgccccctccagcaaagacctcaaatttggtatcgatcgaattttgtctgcggaatttgacccaaaagttaaggaaggcaacactctgagagatctcacatcgctgctaaccggtgggcggcctgcaggggtgcatctagctggcctgcagccttcagcgggacagttcttcgcatctctagatcccattagcgaggcgtctgccatcctcagccccttaagctccaacccaagaaattctgttcagcatcaattccaagacacatttccaggtccctatgctgtgctcactaaggacaccatgccacagacatacaagcggaagcgctcgtggtcccgtgctgtcttttccaacctgcaaagaaaaggcctggagaagaggtttgagatccagaagtacgtgaccaagccagaccgaaagcagctggcggccatgctgggcctcacggatgcacaggtgaaggtgtggttccagaaccggaggatgaagtggcggcactctaaggaggcgcaggcgcagaaggacaaggacaaggaagcgggcgagaagccttccggcggagtccccgccgagggtgaacgggaggagaggagccccagccgttctgagggcgaagccgagagcgagagcagtgactcggagtccctggacatggcccccagcgacacggaacggactgagggaacggagaggtccctgcatcaaaccacggtcatcaaggcctcagccgcgggtgccctcatcactgccagcagcagcacgagtggtagcagcttcagtttcagcagcaccagcagcctgggtagcggcaacacccacgtggggagcgccagcagccttggcggcaactgctcggagctgccttcggctcaccagccctcagtcaccagcagtcctcaaagcccggagatcgcccaggccccgcttgcaggtttatagactggaccgagggcatagcgtgcagcttcctctacactggctggactttgtgcctactttaagttggtggcgagggttgcctagattggagccggacgccacgggcagcctcttcggggttcatactttttctcttgctgccccggctgccagagcccccacccccgtccgcccagtggagagttttaaggttccccctcccccgtcacaaagaaagaattcaactgttttccctacctaagcagctcacactgtgaaatgttcggtggaactgttcctctgagcaccaggggtcttggcataaggacttgctgcacttaggaggcctttaacttgtaaataaaatgtttactacggtttgtaaaaaaaaaaaaaaaaaaaaaaaaaa

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