LOCUS       NM_001407192            4433 bp    mRNA    linear   ROD 28-MAY-2024
DEFINITION  Mus musculus protein-L-isoaspartate (D-aspartate)
            O-methyltransferase 1 (Pcmt1), transcript variant 11, mRNA.
ACCESSION   NM_001407192
VERSION     NM_001407192.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ling,Z., Zhou,S., Zhou,Y., Zhong,W., Su,Z. and Qin,Z.
  TITLE     Protective role of madecassoside from Centella asiatica against
            protein L-isoaspartyl methyltransferase deficiency-induced
            neurodegeneration
  JOURNAL   Neuropharmacology 246, 109834 (2024)
   PUBMED   38181970
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Kalailingam,P., Mohd-Kahliab,K.H., Ngan,S.C., Iyappan,R.,
            Melekh,E., Lu,T., Zien,G.W., Sharma,B., Guo,T., MacNeil,A.J.,
            MacPherson,R.E., Tsiani,E.L., O'Leary,D.D., Lim,K.L., Su,I.H.,
            Gao,Y.G., Richards,A.M., Kalaria,R.N., Chen,C.P., McCarthy,N.E. and
            Sze,S.K.
  TITLE     Immunotherapy targeting isoDGR-protein damage extends lifespan in a
            mouse model of protein deamidation
  JOURNAL   EMBO Mol Med 15 (12), e18526 (2023)
   PUBMED   37971164
REFERENCE   3  (bases 1 to 4433)
  AUTHORS   Zhang,C., Guo,Z.F., Liu,W., Kazama,K., Hu,L., Sun,X., Wang,L.,
            Lee,H., Lu,L., Yang,X.F., Summer,R. and Sun,J.
  TITLE     PIMT is a novel and potent suppressor of endothelial activation
  JOURNAL   Elife 12, e85754 (2023)
   PUBMED   37070640
  REMARK    GeneRIF: PIMT is a novel and potent suppressor of endothelial
            activation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4433)
  AUTHORS   Qin,Z., Dimitrijevic,A. and Aswad,D.W.
  TITLE     Accelerated protein damage in brains of PIMT+/- mice; a possible
            model for the variability of cognitive decline in human aging
  JOURNAL   Neurobiol Aging 36 (2), 1029-1036 (2015)
   PUBMED   25465735
  REMARK    GeneRIF: PIMT levels may significantly influence the course of
            age-related central nervous system dysfunction.
REFERENCE   5  (bases 1 to 4433)
  AUTHORS   Dimitrijevic,A., Qin,Z. and Aswad,D.W.
  TITLE     Isoaspartyl formation in creatine kinase B is associated with loss
            of enzymatic activity; implications for the linkage of isoaspartate
            accumulation and neurological dysfunction in the PIMT knockout
            mouse
  JOURNAL   PLoS One 9 (6), e100622 (2014)
   PUBMED   24955845
  REMARK    GeneRIF: Loss of normal CKB structure and function contributes to
            the mechanisms by which isoaspartate accumulation leads to central
            nervous system dysfunction in the PIMT-Knockout mouse.
            Erratum:[PLoS One. 2014;9(12):e116556]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4433)
  AUTHORS   MacLaren,D.C. and Clarke,S.
  TITLE     Rapid mapping of genomic P1 clones: the mouse
            L-isoaspartyl/D-aspartyl methyltransferase gene
  JOURNAL   Genomics 35 (2), 299-307 (1996)
   PUBMED   8661142
REFERENCE   7  (bases 1 to 4433)
  AUTHORS   Galus,A., Lagos,A., Romanik,E.A. and O'Connor,C.M.
  TITLE     Structural analysis of transcripts for the protein L-isoaspartyl
            methyltransferase reveals multiple transcription initiation sites
            and a distinct pattern of expression in mouse testis:
            identification of a 5'-flanking sequence with promoter activity
  JOURNAL   Arch Biochem Biophys 312 (2), 524-533 (1994)
   PUBMED   8037467
REFERENCE   8  (bases 1 to 4433)
  AUTHORS   Warden,C.H., Mehrabian,M., He,K.Y., Yoon,M.Y., Diep,A., Xia,Y.R.,
            Wen,P.Z., Svenson,K.L., Sparkes,R.S. and Lusis,A.J.
  TITLE     Linkage mapping of 40 randomly isolated liver cDNA clones in the
            mouse
  JOURNAL   Genomics 18 (2), 295-307 (1993)
   PUBMED   8288233
REFERENCE   9  (bases 1 to 4433)
  AUTHORS   MacLaren,D.C., O'Connor,C.M., Xia,Y.R., Mehrabian,M., Klisak,I.,
            Sparkes,R.S., Clarke,S. and Lusis,A.J.
  TITLE     The L-isoaspartyl/D-aspartyl protein methyltransferase gene (PCMT1)
            maps to human chromosome 6q22.3-6q24 and the syntenic region of
            mouse chromosome 10
  JOURNAL   Genomics 14 (4), 852-856 (1992)
   PUBMED   1478665
REFERENCE   10 (bases 1 to 4433)
  AUTHORS   Romanik,E.A., Ladino,C.A., Killoy,L.C., D'Ardenne,S.C. and
            O'Connor,C.M.
  TITLE     Genomic organization and tissue expression of the murine gene
            encoding the protein beta-aspartate methyltransferase
  JOURNAL   Gene 118 (2), 217-222 (1992)
   PUBMED   1511895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC156391.6.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14995044.298205.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-676               AC156391.6         95232-95907
            677-781             AC156391.6         127235-127339
            782-813             AC156391.6         128769-128800
            814-918             AC156391.6         131961-132065
            919-1039            AC156391.6         135575-135695
            1040-1125           AC156391.6         136260-136345
            1126-1301           AC156391.6         138165-138340
            1302-4433           AC156391.6         145414-148545
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 2.26 cM"
     gene            1..4433
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="protein-L-isoaspartate (D-aspartate)
                     O-methyltransferase 1"
                     /db_xref="GeneID:18537"
                     /db_xref="MGI:MGI:97502"
     exon            1..676
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    601..603
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="upstream in-frame stop codon"
     CDS             652..1314
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /EC_number="2.1.1.77"
                     /note="isoform 16 is encoded by transcript variant 11;
                     protein-L-isoaspartate(D-aspartate) O-methyltransferase;
                     protein-beta-aspartate methyltransferase; L-isoaspartyl
                     protein carboxyl methyltransferase; protein
                     L-isoaspartyl/D-aspartyl methyltransferase"
                     /codon_start=1
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase isoform 16"
                     /protein_id="NP_001394121.1"
                     /db_xref="GeneID:18537"
                     /db_xref="MGI:MGI:97502"
                     /translation="

MNTVPSFAENGIIKTDKVFEVMLATDRSHYAKSNPYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGNSGKVIGIDHIKELVDDSITNVKKDDPMLLSSGRVRLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSVKMKPLMGVIYVPLTDKEKQWSRWKDEL"

     misc_feature    679..1269
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="Protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase (PCMT); Region: PCMT; pfam01135"
                     /db_xref="CDD:395902"
     exon            677..781
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            782..813
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            814..918
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1039
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1125
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1301
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..4433
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1568..1573
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="hexamer: AAGAAA"
     polyA_site      1593
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="major polyA site"
     regulatory      2881..2886
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="hexamer: AATAAA"
     polyA_site      2898
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="major polyA site"
     regulatory      4411..4416
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="hexamer: AATAAA"
     polyA_site      4433
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
ORIGIN      

gtcgcttgcgcgggggccgaggactgggggcttcaggccgccccgctgggccccggagctagcgctccgtttgtggcctcccgaccgagactcgctcgcccgcttccccgagccggggtgagtgtggcccagagagtggagtctgtaccaccacgttctggctccgcacggagacgcgggcgcgtcgcggcgagcgtgggtcgcaccaggaaagggcggtgaacggcgctccgagaccgcggacgtcacgaggcaagatgtctagagcttgaataaaactcgagtcttccagagctgggagcgaatgtggttttggaactagccagatggagtgttcttgaaactggacctgtggatttgcagagttcggtccagcggatctgcaggagaggtcattgtagccataacgagtaacggggccgctgtcagaagttctgtttctcttgaaattgaatctgctgttcgggatgcatcactgactttggtcttttaatgcattgttctcttttgcgggggattaggtccacgtagaggatctctaggccgcagcgtgtgtgccaaaactcccattctcctaccccactccacctttcctagtgctgagaatctaaatggtgccaaccaacactgggctgtctatcacctgtaaatatgaacacagtccccagttttgctgagaatggaatcatcaagacagataaagtatttgaagtgatgctggctacagaccgttcccactatgcaaaatctaacccttacatggattctccacaatcaataggtttccaggcaacaatcagtgcccctcatatgcacgcatatgcactagaacttctgtttgaccagctgcatgaaggtgctaaagccctggacgtgggctctggaagtggaatcctcaccgcctgttttgcacggatggttggaaatagtggaaaagtcattggaattgatcacattaaagaactagtagatgactcaataactaatgtcaaaaaggatgacccaatgctcctgtcctcggggcgagtgcggctggtggtgggtgatggaagaatgggatacgctgaagaagccccttacgacgctattcatgtgggagctgcagccccagttgtgccccaggcattaatagaccagttaaagcctggtggaagattgatattgccagtcggtcctgcaggaggaaaccaaatgttggagcagtatgacaagctacaagatggcagtgtcaaaatgaaacctctcatgggggtgatatacgtgcctttaacagataaagaaaagcagtggtccaggtggaaggatgaactgtgaaagcaacgtgagcttgaccagtctataaaccgcagtgggccgctcctctcggtccttacagcatttgagaaccaacagcatcacagcttgttttggactttgttacactcttactttcagcatgaaaacgtgtggttttgtaggatttcctaattccaaacggcgcggagccaaactgatagaggaaggctgcagagcgttaactgcgtgtgttgttttaacatgcccacatttcactttaaaaaaatattaaaagaaagagttctccaactggaaaaattagttttgaagagtggctttgcttttcttgggcacttaattctgttccaatattaattcatcagatttattttgtgcatcagataacaccacattcacaagtttagcttctttggtagccaatgtctgttagatgctactaagaaaacagagatgagctttcttcttaaaagcctttgatgtggcgtcataggatagcatgtcgtagatacaatcagctgctgttaccttaaaactaggtgtttgtaaatactgaaacttgagacgacgatgatggcaggtggtgtcccaaaaacactcagctgttccgattggaaattttgatgtaaatttcctctctctaaaaatgctaggaggcttgtagctcaatatcatttatttgtttctagtttgctgctggtaatgtatctgcatctaacattctgtgtaagcggtcgtgtctttgcacagtttatgcagcgctgctttgccaaataaaattaaaagcaaaagaagcattggtggcctttgaatctagaaggacacaggtctgctaggtccagatgctgcccacagtcctggggaagcgtcgtagtcttatgatattctaggaggtagatatgcatagcattcgtccactctatgtggctgcatttcttcaggatggagactctgtgttcattgtttctctctgccttcctagcccaaggcttgctttttaatagctctgtagtaaatgctcaatgagtgactgggtgaatgtagtatgaggaagaatgtggttgagagtaaacatactgaaatccatgtctcatctataaatagattcattaatttaataactgtaaaatgtctgtgaaggcaatggcactttgaagatcttatttaaatatggttttatattttctaataagactttagttttttgtgaagatcctagaacttgagagaagattagaagctcccggtaaatggggctaagcaaagcgaagccttccctctatgtgtaagggcacatgattctgacaagaagactcttagtgtctgagaagcccatccccactcttcacatcttaaattactgatttaaaagtcatgcagacatcacctgacaagtgtctcttcttggcttaggtctcagtcccaggaaactagcattactgtggtcccgcagacttgaaaaccttaaccagctttggtttgctgctggttgttgctttggaatcctgtcagtgaccccctggagtccaggatgcatggtcatttgaaatatgtgtaaagtcaaatgttcactcaggctttaaagcaaataaattcttaaacttacccaccagtctggatccttggtagtcattgcacattacagcacgcaaatctctgtgctgggcttcaggacggaagaggtgcttccaggtcagtttgccgtccagtggcggggacagtttataaataggcaccatacacttcacccagtttgtacagaaactctgtgcccaagccttgctcttagacttgacacttttaggaggtgaaactttaggatgttgaatgaacagccagtttgaacagcagcactaagtgggtagagatagaagaatggtgttaatgaggtctaagccctttaaaagagttgaatttatatatgtgtttaagacaggtaagaaatgcctgattttcagtgtgcataatttccagagcattttattttcctcatagaaagtacatctgaatacacataatgtaaagggcaatgaagatacttcgagtcattataggtagtgtgatcttgtgaataaactcttcctataaatttgctcttcctagaagctaaatctagttgtcagacttgggttgcagcaataagcttatgtcagggttgagtttctcttcagggctgcttgctctttcccagccaggaacaggacaaatcggcacccagggaaataactactactggccacaccaatacttaggacttcatcccccttgcagctgccatgttttattgtttgtttgtttatttgtggcaggtgggggtggatagcaagatggctcggggtaaagaggcttgctgccaaacctgaagacctgagtttcacccctcccccctcccagacccacagagtagaaggacagctgacacctgcagattgttctctgaccttcaggcatggtagcacttgcctgaaatcccagcacttggaagcaggaagggtcagaagtcatcctaggttacacaagttcaaggccagcccgaatttcatgagagcctgccttaaaggaaagaaaaaaccaaaaagtttctacagtgcatctcttcactaaagcatcgtaagcattgagtggattctaaaggagccatttttaaagtaataactttgtaaactcttcaagctgttcttgaaggaattcctcagagtgtttaaggccttttatctctctagagttttctaatttctggtagtttagtgtggcatcaaagaaagtcatgacagtgttgtcacccagcactgatagacaaatagtaagcatgagtcatcttataaattgttgtagaactcaaccggctgataacttattttaggtaagcacttaaactcttgtagcatacagatcatttcttatcgaatgctgtgttcctgatgccctaatatcttaatgtattctgtagtggagatgggccctgactggactttggttgcatctctgagcttccaccatctttcagaaagatggtagtattttgcccttagcattgttaaaggttctcactcagtaaggggacccacttgaaaagggtactggcccaaggaagcctgggactgtgcactggaatataataaagtggaaatgaagtctaa

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