2025-04-05 10:46:50, GGRNA.v2 : RefSeq release 228 (Jan, 2025)
LOCUS NM_001364321 6023 bp mRNA linear ROD 31-DEC-2024 DEFINITION Mus musculus piwi-like RNA-mediated gene silencing 2 (Piwil2), transcript variant 2, mRNA. ACCESSION NM_001364321 XM_006519328 VERSION NM_001364321.1 KEYWORDS RefSeq. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 6023) AUTHORS Gao,J., Jing,J., Shang,G., Chen,C., Duan,M., Yu,W., Wang,K., Luo,J., Song,M., Chen,K., Chen,C., Zhang,T. and Ding,D. TITLE TDRD1 phase separation drives intermitochondrial cement assembly to promote piRNA biogenesis and fertility JOURNAL Dev Cell 59 (20), 2704-2718 (2024) PUBMED 39029469 REFERENCE 2 (bases 1 to 6023) AUTHORS Lu,X., Zhu,Q., Du,H., Gu,M. and Li,X. TITLE PIWIL2 restrains the progression of thyroid cancer via interaction with miR-146a-3p JOURNAL BMC Endocr Disord 23 (1), 184 (2023) PUBMED 37641092 REMARK GeneRIF: PIWIL2 restrains the progression of thyroid cancer via interaction with miR-146a-3p. Erratum:[BMC Endocr Disord. 2024 Nov 26;24(1):255. doi: 10.1186/s12902-024-01786-z. PMID: 39587564] Publication Status: Online-Only REFERENCE 3 (bases 1 to 6023) AUTHORS Gasperini,C., Tuntevski,K., Beatini,S., Pelizzoli,R., Lo Van,A., Mangoni,D., Cossu,R.M., Pascarella,G., Bianchini,P., Bielefeld,P., Scarpato,M., Pons-Espinal,M., Sanges,R., Diaspro,A., Fitzsimons,C.P., Carninci,P., Gustincich,S. and De Pietri Tonelli,D. TITLE Piwil2 (Mili) sustains neurogenesis and prevents cellular senescence in the postnatal hippocampus JOURNAL EMBO Rep 24 (2), e53801 (2023) PUBMED 36472244 REMARK GeneRIF: Piwil2 (Mili) sustains neurogenesis and prevents cellular senescence in the postnatal hippocampus. REFERENCE 4 (bases 1 to 6023) AUTHORS Ramakrishna,N.B., Battistoni,G., Surani,M.A., Hannon,G.J. and Miska,E.A. TITLE Mouse primordial germ-cell-like cells lack piRNAs JOURNAL Dev Cell 57 (23), 2661-2668 (2022) PUBMED 36473462 REFERENCE 5 (bases 1 to 6023) AUTHORS Lowe,M.G., Yen,M.R., Hsu,F.M., Hosohama,L., Hu,Z., Chitiashvili,T., Hunt,T.J., Gorgy,I., Bernard,M., Wamaitha,S.E., Chen,P.Y. and Clark,A.T. TITLE EED is required for mouse primordial germ cell differentiation in the embryonic gonad JOURNAL Dev Cell 57 (12), 1482-1495 (2022) PUBMED 35679863 REFERENCE 6 (bases 1 to 6023) AUTHORS Kuramochi-Miyagawa,S., Kimura,T., Ijiri,T.W., Isobe,T., Asada,N., Fujita,Y., Ikawa,M., Iwai,N., Okabe,M., Deng,W., Lin,H., Matsuda,Y. and Nakano,T. TITLE Mili, a mammalian member of piwi family gene, is essential for spermatogenesis JOURNAL Development 131 (4), 839-849 (2004) PUBMED 14736746 REFERENCE 7 (bases 1 to 6023) AUTHORS Sasaki,T., Shiohama,A., Minoshima,S. and Shimizu,N. TITLE Identification of eight members of the Argonaute family in the human genome JOURNAL Genomics 82 (3), 323-330 (2003) PUBMED 12906857 REFERENCE 8 (bases 1 to 6023) AUTHORS Tan,C.H., Lee,T.C., Weeraratne,S.D., Korzh,V., Lim,T.M. and Gong,Z. TITLE Ziwi, the zebrafish homologue of the Drosophila piwi: co-localization with vasa at the embryonic genital ridge and gonad-specific expression in the adults JOURNAL Mech Dev 119 Suppl 1, S221-S224 (2002) PUBMED 14516689 REFERENCE 9 (bases 1 to 6023) AUTHORS Kuramochi-Miyagawa,S., Kimura,T., Yomogida,K., Kuroiwa,A., Tadokoro,Y., Fujita,Y., Sato,M., Matsuda,Y. and Nakano,T. TITLE Two mouse piwi-related genes: miwi and mili JOURNAL Mech Dev 108 (1-2), 121-133 (2001) PUBMED 11578866 REFERENCE 10 (bases 1 to 6023) AUTHORS Wang,P.J., McCarrey,J.R., Yang,F. and Page,D.C. TITLE An abundance of X-linked genes expressed in spermatogonia JOURNAL Nat Genet 27 (4), 422-426 (2001) PUBMED 11279525 COMMENT VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122268.5, AB032605.1 and AC025669.19. On Jun 14, 2018 this sequence version replaced XM_006519328.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.162413.1, SRR1660815.201434.1 [ECO:0000332] RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375 [ECO:0006172] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1259 AC122268.5 59963-61221 c 1260-6020 AB032605.1 153-4913 6021-6023 AC025669.19 125171-125173 c FEATURES Location/Qualifiers source 1..6023 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6" /db_xref="taxon:10090" /chromosome="14" /map="14 36.32 cM" gene 1..6023 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /note="piwi-like RNA-mediated gene silencing 2" /db_xref="GeneID:57746" /db_xref="MGI:MGI:1930036" exon 1..1259 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" misc_feature 1080..1082 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /note="upstream in-frame stop codon" CDS 1092..4220 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /note="isoform 2 is encoded by transcript variant 2; Miwi like; piwi-like protein 2; Miwi likw; piwi-like homolog 2" /codon_start=1 /product="piwi-like protein 2 isoform 2" /protein_id="NP_001351250.1" /db_xref="GeneID:57746" /db_xref="MGI:MGI:1930036" /translation="
MSLRLACATESGPGKSKTHPSGAFCSSSLFPFSPQHLSSFHFLSEIPLIVYDHRVQIRNWNRTNSRFYSLSMDPVRPLFRGPTPVHPSQCVRMPGCWPQAPRPLEPAWGRAGPAGRGLVFRKPEDSSPPLQPVQKDSVGLVSMFRGMGLDTAFRPPSKREVPPLGRGVLGRGLSANMVRKDREEPRSSLPDPSVLAAGDSKLAEASVGWSRMLGRGSSEVSLLPLGRAASSIGRGMDKPPSAFGLTARDPPRLPQPPALSPTSLHSADPPPVLTMERKEKELLVKQGSKGTPQSLGLNLIKIQCHNEAVYQYHVTFSPSVECKSMRFGMLKDHQSVTGNVTAFDGSILYLPVKLQQVVELKSQRKTDDAEISIKIQLTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDSVLDVMHAIYQQNKEHFQDECSKLLVGSIVITRYNNRTYRIDDVDWNKTPKDSFVMSDGKEITFLEYYSKNYGITVKEDDQPLLIHRPSERQNNHGMLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLTQQINLSPKQHHGALECLLQRISQNETASNELTRWGLSLHKDVHKIEGRLLPMERINLRNTSFVTSEDLNWVKEVTRDASILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRISPPAWVELKDDRIETYIRTIQSLLGVEGKIQMVVCIIMGTRDDLYGAIKKLCCVQSPVPSQVINVRTIGQPTRLRSVAQKILLQMNCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKYYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFDNYHPKMVVFVVQKKISTNLYLAAPDHFVTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQILHHEPAIQLCGNLFFL"
misc_feature <1308..1904 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /note="large tegument protein UL36; Provisional; Region: PHA03247" /db_xref="CDD:223021" misc_feature 2313..2456 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /note="Argonaute linker 1 domain; Region: ArgoL1; pfam08699" /db_xref="CDD:462567" misc_feature 2457..2810 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /note="PAZ domain, Piwi_like subfamily. In multi-cellular organisms, the Piwi protein appears to be essential for the maintenance of germline stem cells. In the Drosophila male germline, Piwi was shown to be involved in the silencing of retrotransposons in the...; Region: PAZ_piwi_like; cd02845" /db_xref="CDD:239211" misc_feature order(2589..2591,2628..2630,2652..2654,2664..2666, 2718..2720,2769..2771,2775..2777) /gene="Piwil2" /gene_synonym="mili; Piwil1l" /note="nucleic acid-binding interface [nucleotide binding]; other site" /db_xref="CDD:239211" misc_feature 2832..4166 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /note="PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of related...; Region: Piwi_piwi-like_Euk; cd04658" /db_xref="CDD:240016" misc_feature order(3339..3341,3351..3353,3387..3398,3405..3407, 3435..3437,3444..3446,3456..3458,3468..3470) /gene="Piwil2" /gene_synonym="mili; Piwil1l" /note="5' RNA guide strand anchoring site [active]" /db_xref="CDD:240016" misc_feature order(3531..3533,3537..3539,3741..3743,4140..4142) /gene="Piwil2" /gene_synonym="mili; Piwil1l" /note="active site" /db_xref="CDD:240016" exon 1260..1496 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 1497..1584 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 1585..1723 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 1724..1930 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 1931..2041 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 2042..2159 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 2160..2284 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 2285..2365 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 2366..2479 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 2480..2668 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 2669..2753 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 2754..2843 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 2844..2984 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 2985..3098 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 3099..3287 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 3288..3389 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 3390..3506 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 3507..3595 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 3596..3701 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 3702..3955 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 3956..4063 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" exon 4064..6023 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /inference="alignment:Splign:2.1.0" regulatory 6001..6006 /regulatory_class="polyA_signal_sequence" /gene="Piwil2" /gene_synonym="mili; Piwil1l" /note="hexamer: AATAAA" polyA_site 6021 /gene="Piwil2" /gene_synonym="mili; Piwil1l" /note="major polyA site" ORIGIN
gcagttgatacaagggtgcaaatgaatgcaaatgtattactacctgcatctgtgtgtatgtattttgtgttagaaacagaaagaactaaagacttgttcatttctttgtgtaaagtggctctgtaggcttcagtcagccttgggattggcttcaattccacaagtaaccttggagaggaaaaaaagtcacttgtcttccctcagtttattttttttaaattgtttttaaacagaagaatgttatcaccatatctctatttgcacagtggttttttttttaatttattaaaatggttactttgcatgagttgggggtacgtagatgtggaggtcagaagacaatttttaggagtcagctctcaactttatgccctgttgatacaggatctcttgtttctgctgctgctttgaatgtggggttcaagttatcaagtgcagccaaggacttttacccactgaaccagtttcctagtttgcttttatttttttataaaaagatttgagtgttctgagtaattttcctgtatgtatgtgtgtgtactaagtgcatgcctagtgtctggaggtcaaaaaagggcatcagattttctggaacttcagttatggatggttgggagctaccacatggatactgggaacccaaaccaggtctgctgcaagaatatgtgctcttaatctgagccatctctctagtccctttcccttccccttttgcttttaaattagagtgattgtaaagactttattgagtatcagataagtccagtaagagacatgtgtgatcagcaagcttttattaagccaatatgtgttgtttatgctctataaagcataggacccttctgtgatttctgcaacttttcccagccacacagaccttgctgccctgtcacagtgggcaagctgcacccttcccccaccccatgtcctttccctcaggtctagactcaactttatccagatgtttctaggtcttacttctgaaatccttcagttctctgagattccctggccactctacataaattagctaactcgggtgcaatgtgctccgcttgttgccagcacttaggatactatagccagcgggtatgagtttgaggctagcctgtgctacagagtcaggtccaggtaaatctaaaactcacccctctggtgctttttgctcctcttcactgtttcccttctctccacagcatttgtcatcctttcatttcttatctgagatcccactgatagtctatgaccacagagtacagataaggaattggaacagaaccaacagtcgtttctacagcctctcaatggatcctgtcaggccgttgttcagggggcccaccccagtccacccatctcagtgtgtgcggatgccaggctgttggcctcaagctcctagacctttggaaccagcttggggtagggcaggacctgcaggcagaggccttgtgtttagaaaaccagaagactccagcccaccactccagccagtacaaaaggattctgtaggtttggtgtccatgttccgtggcatgggtcttgacacagcattccggcctccttcaaaacgagaagtgcctcctttaggcagaggagttctaggtcgaggcttgtctgctaacatggtccgcaaggacagagaagaaccccgttcctctttgcctgatccttcggtgctggcagctggggacagcaaactggcagaggcttctgttggttggagtagaatgctgggaagaggtagttctgaggtctctctgttaccactgggacgagcagccagtagtataggcagaggaatggacaaacctcccagtgcctttggcctcacagctcgggatcccccacggctgccacagcctccagctctgtctccaacttcactgcactctgccgatccccctccggtcctgactatggaacgaaaggaaaaagagcttttggtcaagcaaggatcaaaaggaactcctcagtctttgggactgaacctcatcaaaatccagtgtcataacgaagcagtttatcagtaccatgtgactttcagccccagtgtggaatgcaaaagcatgaggtttggcatgttgaaggaccaccagtctgtcactggaaacgtcactgcttttgatggctctattctttatcttcctgttaagcttcaacaagttgttgagttaaaaagtcagaggaaaactgacgatgccgagatcagtatcaagattcagctgacaaagatcctggagccgtgttctgacctgtgcatccccttctacaatgttgtcttccggcgggtaatgaaacttctggatatgaagcttgtggggagaaacttctatgaccctacaagtgccatggtactgcagcaacacagattgcagatctggcctggctatgcggctagtatccggaggacagacgggggtctcttcctgctcgctgatgtctctcataaggtcattcggaacgactctgtgctggatgtcatgcatgctatctaccagcagaacaaggagcacttccaggacgagtgcagcaagcttctggttggcagcattgtcatcacgcgctacaacaatcgtacctaccgaatcgatgatgtggactggaacaagacccctaaagacagctttgtcatgtcggacgggaaggaaatcacattcctggaatactacagcaaaaactatgggatcacagtcaaggaagatgaccagccgctgctgatccaccggcccagtgagagacagaataaccatggcatgttgctgaagggcgagatcctgctgctgcccgagctctccttcatgacggggatccctgagaagatgaagaaggacttcagggccatgaaggacttgactcagcagattaacctgagccccaagcagcaccacggtgctttggaatgcctgctgcagagaatttcacaaaacgagacagccagcaatgagctgacccgctgggggctcagtctgcataaagatgtccacaagattgaaggtcggcttctgccaatggagaggatcaacttaaggaacacttcatttgtcacatcggaggacctgaactgggttaaggaagtgaccagagatgcttccattctaactattcccatgcatttctgggcactcttttatccaaagagagcaatggaccaagccagagaactggttaacatgttggaaaagattgccgggcccattggcatgcgcataagccccccagcctgggttgagctgaaggatgaccgaatagagacctatatcaggaccattcagtccttactgggagttgaggggaagatacaaatggtcgtttgcatcatcatgggcacacgtgatgatctctatggagccatcaagaagctgtgctgcgtgcagtccccagtgccctcacaggtcatcaatgtccgaaccattggtcagcccaccaggcttcggagcgtggctcagaaaattttacttcagatgaactgtaaactgggtggtgagctctggggagtggatattccgctgaaacaactaatggtgattggaatggatgtgtaccatgaccccagcagaggcatgcgctctgtggtcggcttcgtggccagcataaatctcacactcaccaaatggtactcgagggtggtgttccagatgccacatcaggagattgtggacagcctgaagctctgcctggtgggttccttgaaaaagtattatgaggtgaaccattgtctcccagagaaaattgtggtgtaccgagatggagtgtctgatggccagctaaagacagttgccaactacgagatccctcagctgcagaagtgttttgaagcctttgataactaccaccccaagatggtggtgtttgtagttcagaagaaaatcagcaccaatctgtaccttgctgctcctgatcacttcgtaaccccctcccccgggactgtggttgatcataccataaccagctgtgagtgggtggatttctaccttcttgcccatcatgtgcgacagggctgtggcatacctacacactacatctgtgttctgaacactgcaaatctgagccctgatcacatgcagaggttgactttcaaactatgccacatgtactggaattggcctggtaccatccgagttccagctccttgcaagtatgcccacaagctagctttcctgtccggacagattttgcatcatgagccagccatccagctgtgtgggaacctgttcttcctgtaactgggaacttggacaccggctgcaaggagcaactggactcagctcagctcctccttacagaatcaacagaaatggcagtggaattttatgtttgcattttctctttctccatccttgtagaattagatttctgttcttcttttaaccctgatatcatagtagggtgttgtggtgcatgccttttatcccagcacttgggagactgaagcaggagtatctttagttcaaggccagcctagactacatggtgagttctaggctagccaagattacagagtgagaccctgtctcaaaaaacaaaaacaaaccactgtcccctcaaagcccacaacaaaaccaaagcctggggtcaaggaagcaagtttgtaggtagccgctggctgcccgctgccttcatggagtgtgtgccgtcagcgctgcttctcctcagccgagcgtggagcttcggacagggcagtgatgacatgttcttagcatgtcaaatccccctaccaaataagtcaagcaaggaaaaaatagcccccaaggcagcctgagcatcagttcctagaggttatgcctacagaaccatcctatttctggtggcagaagtgacatgaagtcattgcagacatcttaaaggagagttactgtgcagctgtctacatgtgtgaaagacatgtagaaaaaccagcgtagggtacagtcgggcgtatgtgcccacctgacccagggctgtgagtctgacttcccgagagtctggctagagctgcttttctggtccttttggttatttgcaaccatcatcagattgctttcctgcagcccgactgatacacgcatgtgcgcacgcacacacttttgttctttgtgactaatcttgaataaattcaattagaacacatggaaaggattcagcagacctaggaacatttggggtggagtgtagtttttctgcaaaagtctgtaaatgagattacgcaagagtctcttccagctgtgggctggtgttgcttggaaaatttcaaaatcccaaagtttcaggcttcccaaagttggcttggaaaaatgtgatagtctcacctgagtctagacatgtaggaaattttcctagggcctctgggcttcagtatttgggggaagcactggttttctgtgttatttttttccttctgtttcagaatcttcaagtttcttcaggcttcagtgggccatccctttactgggctctaaaagctaattttacttaaccttttcaaatgtgtatgtatctatttatgttttggtgtggtggatggatggtaggggactgagcagaaatagtcattttaaataacagtgtgctaggagagcctcagtgtgaagtcctgaggagcagcgggggctgtgggagtcagtgtcagccctcactcagacaggccaagcctgggctcgaagacaacattgtccagggaagcctttagtttgctatagcacccagaggttggcgaggaataagggttagggtcctcaaatacacccatggcttttggagtctatgaccaaggccaggctgagacctgaaatgtaaaagccatagaattaaacagaacagactgaaaaacgagtcttaagttccactttctggatttctctggaagtcttttgaatttgtctgtagaagttgctgttcctagcactccttttctctttaggtagaacagatacttgaccataatgccagaatgtactttctctgccttggtttttctatgccttgtttttcagtttcagggccaaacaattgggccctgtggtgtaaaataaaaacaatgtatgtgtata
//
by
@meso_cacase at
DBCLS
This page is licensed under a
Creative Commons Attribution 4.0 International License (CC BY 4.0).
If you use GGRNA in your work, please cite:
Naito Y, Bono H. (2012)
GGRNA: an ultrafast, transcript-oriented search engine for genes and transcripts.
Nucleic Acids Res., 40, W592-W596.
[Full Text]