LOCUS       NM_131773               3544 bp    mRNA    linear   VRT 14-DEC-2025
DEFINITION  Danio rerio claudin 12 (cldn12), mRNA.
ACCESSION   NM_131773
VERSION     NM_131773.2
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Zhang,F., Tian,J., Li,J., Zhang,T. and Wang,X.
  TITLE     ILA mitigates HFD-induced metabolic dysfunction via lipid-immune
            crosstalk and gut microbiota modulation in zebrafish
  JOURNAL   Fish Shellfish Immunol 167, 110683 (2025)
   PUBMED   40865746
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Zhang,F.L., Yang,Y.L., Zhang,Z., Yao,Y.Y., Xia,R., Gao,C.C.,
            Du,D.D., Hu,J., Ran,C., Liu,Z. and Zhou,Z.G.
  TITLE     Surface-Displayed Amuc_1100 From Akkermansia muciniphila on
            Lactococcus lactis ZHY1 Improves Hepatic Steatosis and Intestinal
            Health in High-Fat-Fed Zebrafish
  JOURNAL   Front Nutr 8, 726108 (2021)
   PUBMED   34722607
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3544)
  AUTHORS   Wu,N., Xu,X., Wang,B., Li,X.M., Cheng,Y.Y., Li,M., Xia,X.Q. and
            Zhang,Y.A.
  TITLE     Anti-foodborne enteritis effect of galantamine potentially via
            acetylcholine anti-inflammatory pathway in fish
  JOURNAL   Fish Shellfish Immunol 97, 204-215 (2020)
   PUBMED   31843701
REFERENCE   4  (bases 1 to 3544)
  AUTHORS   Guo,X., Li,J., Ran,C., Wang,A., Xie,M., Xie,Y., Ding,Q., Zhang,Z.,
            Yang,Y., Duan,M. and Zhou,Z.
  TITLE     Dietary nucleotides can directly stimulate the immunity of
            zebrafish independent of the intestinal microbiota
  JOURNAL   Fish Shellfish Immunol 86, 1064-1071 (2019)
   PUBMED   30590163
REFERENCE   5  (bases 1 to 3544)
  AUTHORS   Shu,Y., Lou,Q., Dai,Z., Dai,X., He,J., Hu,W. and Yin,Z.
  TITLE     The basal function of teleost prolactin as a key regulator on ion
            uptake identified with zebrafish knockout models
  JOURNAL   Sci Rep 6, 18597 (2016)
   PUBMED   26726070
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3544)
  AUTHORS   Kumai,Y., Bahubeshi,A., Steele,S. and Perry,S.F.
  TITLE     Strategies for maintaining Na balance in zebrafish (Danio rerio)
            during prolonged exposure to acidic water
  JOURNAL   Comp Biochem Physiol A Mol Integr Physiol 160 (1), 52-62 (2011)
   PUBMED   21600298
REFERENCE   7  (bases 1 to 3544)
  AUTHORS   Clelland,E.S. and Kelly,S.P.
  TITLE     Exogenous GDF9 but not Activin A, BMP15 or TGFbeta alters tight
            junction protein transcript abundance in zebrafish ovarian
            follicles
  JOURNAL   Gen Comp Endocrinol 171 (2), 211-217 (2011)
   PUBMED   21291886
REFERENCE   8  (bases 1 to 3544)
  AUTHORS   Clelland,E.S. and Kelly,S.P.
  TITLE     Tight junction proteins in zebrafish ovarian follicles: stage
            specific mRNA abundance and response to 17beta-estradiol, human
            chorionic gonadotropin, and maturation inducing hormone
  JOURNAL   Gen Comp Endocrinol 168 (3), 388-400 (2010)
   PUBMED   20553723
REFERENCE   9  (bases 1 to 3544)
  AUTHORS   Woods,I.G., Wilson,C., Friedlander,B., Chang,P., Reyes,D.K.,
            Nix,R., Kelly,P.D., Chu,F., Postlethwait,J.H. and Talbot,W.S.
  TITLE     The zebrafish gene map defines ancestral vertebrate chromosomes
  JOURNAL   Genome Res 15 (9), 1307-1314 (2005)
   PUBMED   16109975
REFERENCE   10 (bases 1 to 3544)
  AUTHORS   Loh,Y.H., Christoffels,A., Brenner,S., Hunziker,W. and Venkatesh,B.
  TITLE     Extensive expansion of the claudin gene family in the teleost fish,
            Fugu rubripes
  JOURNAL   Genome Res 14 (7), 1248-1257 (2004)
   PUBMED   15197168
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JBMGRA010000016.1 and EH545623.1.
            
            On Feb 18, 2016 this sequence version replaced NM_131773.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR32588723.3537420.1, AF359431.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA4476787 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                JBMGRA010000016.1  47670156-47670181   c
            27-193              EH545623.1         40-206
            194-2835            JBMGRA010000016.1  47664396-47667037   c
            2836-3543           EH545623.1         208-915
            3544-6185           JBMGRA010000016.1  47664396-47667037   c
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="16"
                     /map="16"
     gene            1..3544
                     /gene="cldn12"
                     /gene_synonym="wu:fc27c11; wu:fc37h01; zgc:86588"
                     /note="claudin 12"
                     /db_xref="GeneID:81593"
                     /db_xref="ZFIN:ZDB-GENE-010328-14"
     exon            1..163
                     /gene="cldn12"
                     /gene_synonym="wu:fc27c11; wu:fc37h01; zgc:86588"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    132..134
                     /gene="cldn12"
                     /gene_synonym="wu:fc27c11; wu:fc37h01; zgc:86588"
                     /note="upstream in-frame stop codon"
     exon            164..462
                     /gene="cldn12"
                     /gene_synonym="wu:fc27c11; wu:fc37h01; zgc:86588"
                     /inference="alignment:Splign:2.1.0"
     CDS             186..1211
                     /gene="cldn12"
                     /gene_synonym="wu:fc27c11; wu:fc37h01; zgc:86588"
                     /codon_start=1
                     /product="claudin-12"
                     /protein_id="NP_571848.1"
                     /db_xref="GeneID:81593"
                     /db_xref="ZFIN:ZDB-GENE-010328-14"
                     /translation="

MSCRDIHATNAFAFVIAMLSVGGLTVATLIPQWRTMRLLTFNRNAKNVTVYDGLWTKCVRRDGSSGCYYFDSDWYAKVDQLDLRLLQFCLPTGLLFSSLALLLCLTGMCKTACCSKTPDDIKNSRCLVNSSGCHLVAGMLLFLGGAIAMPPSVWFLFHTRHLNERYDHLFAVEFGVYVAIGSAGGLILAALLMFMWYCMCKKLPSPFWLPLPEVPTMTQSLSAQPLMTNGLPTPVTYAPQSFPPAVLDAPAFVPMQGYPQPASTQPMPPHVYMPQMSVPDGYGSEVGASQVYSYAPSQSYAPSQGYAPSQSYAPSQRYAGHRYSTRSRTSGIEIDIPVLTD"

     misc_feature    <786..>1130
                     /gene="cldn12"
                     /gene_synonym="wu:fc27c11; wu:fc37h01; zgc:86588"
                     /note="Atrophin-1 family; Region: Atrophin-1; pfam03154"
                     /db_xref="CDD:460830"
     exon            463..3544
                     /gene="cldn12"
                     /gene_synonym="wu:fc27c11; wu:fc37h01; zgc:86588"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

agtgacgtactgccgcgcctgcggtcagtgtgttgtgcgcgctcccgaagaagaagagtgttcatacaacacggtggaacccaacgacagaaaaccaccgatttaagtgagaaaatgactgctacatcacctaaacattcattaaacgctcgacattaatcaggtatcatttgctgacttcagccatgtcttgtcgtgacattcacgccaccaatgcgttcgcctttgttattgcgatgctgtcggtcggtggtttgaccgttgctacactcattcctcagtggagaaccatgcgcctgcttaccttcaaccgtaatgccaaaaatgtgactgtatacgatggcctgtggaccaagtgtgtacggcgggatggctcttcaggctgctattactttgactcagactggtacgcaaaagtagaccagttggacctcagattgctgcaattctgtttacctactggtctgctgttttccagcttggctcttctgctctgtcttactggcatgtgtaaaactgcttgctgctccaaaacccctgacgacatcaagaactctcgctgcttggtcaacagctcaggctgccatctagtagctgggatgcttctgtttctaggtggagcgatcgccatgccaccctccgtctggttcctcttccacacccgtcacctaaatgagcgctacgatcatttatttgctgttgagtttggggtgtatgtggccatcggcagtgctggtggtttaatcctggctgctctgctgatgttcatgtggtattgtatgtgtaaaaaactgccttcgcctttctggctgcctcttcctgaagtgcccacaatgacccaaagcctctctgcgcagcctctcatgaccaacggtttgcctactcccgtgacgtacgcgccccagagcttccctccagccgttctggacgcccctgcttttgtcccaatgcagggttaccctcaacctgcatccacacagcctatgcccccccacgtctacatgccacaaatgtctgtgccagatggttatggctcagaagttggagcctcgcaggtttatagttatgccccctcacaaagctacgctccttctcagggatatgctccatcacaaagctacgcgccatcacagaggtacgccggacaccgatattcgacacgctcccgaacgtcagggattgagattgacattcctgtcctcacagattgagattggctgtctaaggatatttcaaattaaaaaggtcatatttagaaattagacaacaactctgatggtaaatttaaagtcatgtaaatggttaaatagaaagaacagccttcatttggttaatgtcgcctctaattcttaggactaaaatggccagaactatcagaaaagtgatttgagcagatagaaaggtgacattctcatataacctcgtattacaactaaagtctgcagtaaagcaactctgaacacatcaaaccttgaagcagatgagctactgcagaagaagagcaaactggtgacactcctgtctgctaagaaaactgaggttcccattgacatgagctcaacaaaactatgacagaaaattgtaggaaggttggctattctgatacatcttaggcccatttcagttctacctcttaaacccttccacttactcttacgcctccaagcaaaagagaatcagtaagcgaaagtgccgtttttattgaatcaaaaaggtcataattagaaattagagaacaactccgatggtaaatttgaagacatgtaaatggttaaattgaaagattagcctttattcagttgatgtcgccggtgcctcttaattattagtactgaaaatggccaaaaaaggaaaaatcgatcaaaaaaattatttgagcagataaaaaggtgacattttctcaaataacctcttattacgattaaagtctgcaggagagcaactctgaacacagcaaaccttgaagtagatgagctacagcagcagcagaagaagagcactctggtgacactcctgtctgctaggaaaactgatgctccaattgacatgaattcaacaaaactcgatatcagatcaggcaaccattttacaatcttctgttaacacttaggcccaatccagccttccactcactgattattttttgcttggaggcgcagtaaggcgaaatgccctccaaatacaaaggtcataattagaaattagagaacaactatgatggtaaatttgaagtcatgtaaattgtaaaattgaaaaataagcctttattcagttgatgtcaccaatgcctcttaattcttggcactgaaaatggccaaaaaaagggaaaaaaacaacccgaaaagcgatttgagcagatagaaaggtgatgttgtctcaaataacctcttactacaactaaagtcttcaggggagcaactctgaacacatcaaaccttgaagcagatgagctacagcagcagaagaagagcgcactagtgacattctggtctgctaagaaatctgatcataattagaaattagagtacaactctcatggtgaatttgaagttatgtaaatggttaaagatacgcctttattcagtggatgtcgcagatgcctcttaattcttagcactaaaatggccaaaaagggaaaaaaggtcatgaaagcagtaacactagctgaagttgtgcatctcgctttataatagtttcatttataaaacacaagcaaaacaaacttgttaataatttcactgagtatgtcaaggtgacttcacttaattcatactcatgtttcgtgttgttactctgcttttaaatgtcagtagaaacactgaatataaagttgattttaagcactgttttaacctttaagcaaatatttctgctaatatctaaatgtgactattacagtaaatattagaaagagatgtaacactgactgctgaaactgtattattctcagtgccatgaagttggacgttttcttttttaagcttagtgaatatgttggtttattgctctaatatctgtacatcatgttttcatggggttttatgcagatgtcacaatgttaaacgaacacctagtagaggttttttatatatacatggaagaatcatctctgcctgatgcctttgtgcagcttaactcttgtttccagaactgtacttccacttattaatttaatcctttataatgagccttacatcaacaagccattaactgaacactgcgttgcattccattgttttcttataaatgtaaatgttatcttattgttgttgttgtgttttaaaggtgtcaccagcgtattcatcattgtgcaactcagcatttgaataatgaatgctccattaatgtgtttgtttactgttagtttcttagctgaattgaggaaaacacgctaatgtcttaaacttgatcttcgcctattgaatgttgcacatatctatttcagtttgcctgtttctgcaaagctctgcagcacaatgttaattttaatcactgtgtagtttactaatgtctttgtttttttattaaagtgcagtttattgaa

//