LOCUS       NM_131040               1823 bp    mRNA    linear   VRT 26-JAN-2025
DEFINITION  Danio rerio engrailed homeobox 2b (en2b), mRNA.
ACCESSION   NM_131040
VERSION     NM_131040.1
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Choi,M.K., Cook,A., Mungikar,K., Eachus,H., Tochwin,A., Linke,M.,
            Gerber,S. and Ryu,S.
  TITLE     Exposure to elevated glucocorticoid during development primes
            altered transcriptional responses to acute stress in adulthood
  JOURNAL   iScience 27 (7), 110160 (2024)
   PUBMED   38989456
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Zhou,J., Yang,Y.J., Gan,R.H., Wang,Y., Li,Z., Zhang,X.J., Gui,J.F.
            and Zhou,L.
  TITLE     Foxl2a and Foxl2b are involved in midbrain-hindbrain boundary
            development in zebrafish
  JOURNAL   Gene Expr Patterns 46, 119286 (2022)
   PUBMED   36341978
REFERENCE   3  (bases 1 to 1823)
  AUTHORS   Branigan,G.L., Olsen,K.S., Burda,I., Haemmerle,M.W., Ho,J.,
            Venuto,A., D'Antonio,N.D., Briggs,I.E. and DiBenedetto,A.J.
  TITLE     Zebrafish Paralogs brd2a and brd2b Are Needed for Proper
            Circulatory, Excretory and Central Nervous System Formation and Act
            as Genetic Antagonists during Development
  JOURNAL   J Dev Biol 9 (4), 46 (2021)
   PUBMED   34842711
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1823)
  AUTHORS   Amblard,I., Thauvin,M., Rampon,C., Queguiner,I., Pak,V.V.,
            Belousov,V., Prochiantz,A., Volovitch,M., Joliot,A. and Vriz,S.
  TITLE     H2O2 and Engrailed 2 paracrine activity synergize to shape the
            zebrafish optic tectum
  JOURNAL   Commun Biol 3 (1), 536 (2020)
   PUBMED   32994473
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1823)
  AUTHORS   Liu,Y., Zhu,Z., Ho,I.H.T., Shi,Y., Li,J., Wang,X., Chan,M.T.V. and
            Cheng,C.H.K.
  TITLE     Genetic Deletion of miR-430 Disrupts Maternal-Zygotic Transition
            and Embryonic Body Plan
  JOURNAL   Front Genet 11, 853 (2020)
   PUBMED   32849832
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1823)
  AUTHORS   Paragas,V.B., Zhang,Y.Z., Haugland,R.P. and Singer,V.L.
  TITLE     The ELF-97 alkaline phosphatase substrate provides a bright,
            photostable, fluorescent signal amplification method for FISH
  JOURNAL   J Histochem Cytochem 45 (3), 345-357 (1997)
   PUBMED   9071316
REFERENCE   7  (bases 1 to 1823)
  AUTHORS   Moens,C.B., Yan,Y.L., Appel,B., Force,A.G. and Kimmel,C.B.
  TITLE     valentino: a zebrafish gene required for normal hindbrain
            segmentation
  JOURNAL   Development 122 (12), 3981-3990 (1996)
   PUBMED   9012518
REFERENCE   8  (bases 1 to 1823)
  AUTHORS   Ekker,M., Speevak,M.D., Martin,C.C., Joly,L., Giroux,G. and
            Chevrette,M.
  TITLE     Stable transfer of zebrafish chromosome segments into mouse cells
  JOURNAL   Genomics 33 (1), 57-64 (1996)
   PUBMED   8617510
REFERENCE   9  (bases 1 to 1823)
  AUTHORS   Hatta,K., Bremiller,R., Westerfield,M. and Kimmel,C.B.
  TITLE     Diversity of expression of engrailed-like antigens in zebrafish
  JOURNAL   Development 112 (3), 821-832 (1991)
   PUBMED   1682127
REFERENCE   10 (bases 1 to 1823)
  AUTHORS   Holland,P.W. and Williams,N.A.
  TITLE     Conservation of engrailed-like homeobox sequences during vertebrate
            evolution
  JOURNAL   FEBS Lett 277 (1-2), 250-252 (1990)
   PUBMED   1980115
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from X68447.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X68447.1, BC071290.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA3505370, SAMEA3505371
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /db_xref="taxon:7955"
                     /chromosome="2"
                     /map="2"
     gene            1..1823
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="engrailed homeobox 2b"
                     /db_xref="GeneID:30238"
                     /db_xref="ZFIN:ZDB-GENE-980526-40"
     exon            1..806
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    311..313
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="upstream in-frame stop codon"
     CDS             338..1123
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="homeobox protein en-2b; engrailed 3; engrailed 2b"
                     /codon_start=1
                     /product="homeobox protein engrailed-2b"
                     /protein_id="NP_571115.1"
                     /db_xref="GeneID:30238"
                     /db_xref="ZFIN:ZDB-GENE-980526-40"
                     /translation="

MEENDHSNRDVERQDSGDESNRAILPLLQAPGNVLPHRITNFYIDNILRPDFGRRKEGSRRDEINIVERENRCPSAPGSGQVAPVSGEGTSSPRAVNASKKTDISTDESLKSRAETGDQCLSSDSDCSQRCAAQAKQPMLWPAWVYCTRYSDRPSSGPRSRKPKKKTPTKEDKRPRTAFTAEQLQRLKNEFQNNRYLTEQRRQALAQELGLNESQIKIWFQNKRAKIKKATGNKNTLAVHLMAQGLYNHATVTKDDKSDSD"

     misc_feature    338..409
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="propagated from UniProtKB/Swiss-Prot (P31533.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    494..712
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="propagated from UniProtKB/Swiss-Prot (P31533.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    791..865
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="propagated from UniProtKB/Swiss-Prot (P31533.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    854..1024
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="Region: Homeodomain; pfam00046"
                     /db_xref="CDD:459649"
     misc_feature    1025..1117
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /note="Engrailed homeobox C-terminal signature domain;
                     Region: Engrail_1_C_sig; pfam10525"
                     /db_xref="CDD:431338"
     exon            807..1819
                     /gene="en2b"
                     /gene_synonym="en3; ENG-3; eng2b; eng3; ZF-EN-1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gcgtttccttatttacttgcgcaggtctactgctttgattctgaatagattacatttccctgtatgttccaaagtagatataaagacaattacaagaggcacaatgtggaattaatttataaaactatgtttttatttttacgcgcacaagacggtttcgtcgcatttacccgacaaaaaaactgaaaaaaggaatttgttctttttcccgttcgtttctttttgcagagaaactcagaaataaggaaaatatactactaaaactggaacacaaacatacttgtgtgcaagagagcgaaaggggtggggttaaaaagaacatttggagagtttcactatggaagaaaatgatcatagcaacagagatgtggagcgccaggattcgggcgacgagtccaatagggctattcttcccctgttgcaggctcctggaaacgtactccctcacagaatcaccaacttttacatcgacaatattttaagaccagactttggtcgcaggaaagaaggaagcagacgcgacgaaattaacatagttgagagagagaaccgctgtccatcggctcccggatcagggcaggtagctccagtatcgggggaaggaacctcgagccctcgcgctgtaaacgcatctaaaaaaactgatattagcacggacgaatctctgaaatcccgtgcagagactggagatcagtgtttaagttccgattcggactgctcacaaagatgcgctgcgcaggcgaaacagccaatgctttggccggcttgggtatattgcacaagatattcagacagaccttcatcaggaccaagatcacgcaaaccaaagaagaaaaccccaaccaaggaagacaagcgtccgagaacagcattcacagcggagcaactacagagactcaagaatgaattccagaataatcgttacctgacggagcaaaggagacaagcgttggcccaggaactcggcctgaacgagtctcaaatcaaaatctggtttcaaaacaaaagggcaaagatcaaaaaagcaacggggaacaaaaacacacttgccgtgcacctgatggcacagggactttacaatcacgccacagtaacaaaggacgacaaatcagacagtgattaacacggaggacaggatatcttaaatgcaataattctaaacaagggccagtgtacaaaataccagcattaaacggatgaaaactatgtatgagatgtgattttgcaatattatatgtatatatactttgtgtagggtggtgtacaacttttttttaattagcatcactgctgtgcactgactgaacttcagatttattgcgcgaggaaaaggacgcttctttatttaaaaaaaaaaaaagaaaaaaaaaggagcgatttgatacacaccttgacgttgagccacacgtgaacgtgtttaatccatgctgaagtccaaagatttttcctgctgcattcaggcgactgtgatttaaaataaatggtaaaaatgttttatctgatagatgtttgtttgtttgcataattttattactggatgaatgttttacttaattgacttgagttacagagacatggtatttaaaaagacaacattatcaacatattccacattaaagaattcagtttatttcatatttatggtctattgtctggcaaggatggtttacctcacatctcaacattttcaggaaaaagatcaacatctgaatgcatacgagtcagtaaaactcaaaaccacactaatgcaagtacttgactttgttgtctgttgaatttagctgggagtgtacaataaaagttatcaactttttcaaaaaaaa

//