LOCUS       NM_001317223            2986 bp    mRNA    linear   VRT 25-JUN-2025
DEFINITION  Danio rerio argonaute RISC component 1 (ago1), mRNA.
ACCESSION   NM_001317223 XM_694292
VERSION     NM_001317223.2
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Raman,R., Ryon,M. and Jagadeeswaran,P.
  TITLE     RNaseH-mediated simultaneous piggyback knockdown of multiple genes
            in adult zebrafish
  JOURNAL   Sci Rep 10 (1), 20187 (2020)
   PUBMED   33214638
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Liu,Y., Zhu,Z., Ho,I.H.T., Shi,Y., Li,J., Wang,X., Chan,M.T.V. and
            Cheng,C.H.K.
  TITLE     Genetic Deletion of miR-430 Disrupts Maternal-Zygotic Transition
            and Embryonic Body Plan
  JOURNAL   Front Genet 11, 853 (2020)
   PUBMED   32849832
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2986)
  AUTHORS   Bayes,A., Collins,M.O., Reig-Viader,R., Gou,G., Goulding,D.,
            Izquierdo,A., Choudhary,J.S., Emes,R.D. and Grant,S.G.
  TITLE     Evolution of complexity in the zebrafish synapse proteome
  JOURNAL   Nat Commun 8, 14613 (2017)
   PUBMED   28252024
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2986)
  AUTHORS   Katz,S., Cussigh,D., Urban,N., Blomfield,I., Guillemot,F.,
            Bally-Cuif,L. and Coolen,M.
  TITLE     A Nuclear Role for miR-9 and Argonaute Proteins in Balancing
            Quiescent and Activated Neural Stem Cell States
  JOURNAL   Cell Rep 17 (5), 1383-1398 (2016)
   PUBMED   27783951
REFERENCE   5  (bases 1 to 2986)
  AUTHORS   Elkon,R., Milon,B., Morrison,L., Shah,M., Vijayakumar,S.,
            Racherla,M., Leitch,C.C., Silipino,L., Hadi,S., Weiss-Gayet,M.,
            Barras,E., Schmid,C.D., Ait-Lounis,A., Barnes,A., Song,Y.,
            Eisenman,D.J., Eliyahu,E., Frolenkov,G.I., Strome,S.E., Durand,B.,
            Zaghloul,N.A., Jones,S.M., Reith,W. and Hertzano,R.
  TITLE     RFX transcription factors are essential for hearing in mice
  JOURNAL   Nat Commun 6, 8549 (2015)
   PUBMED   26469318
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2986)
  AUTHORS   McFarlane,L., Svingen,T., Braasch,I., Koopman,P., Schartl,M. and
            Wilhelm,D.
  TITLE     Expansion of the Ago gene family in the teleost clade
  JOURNAL   Dev Genes Evol 221 (2), 95-104 (2011)
   PUBMED   21556854
REFERENCE   7  (bases 1 to 2986)
  AUTHORS   Sander,J.D., Dahlborg,E.J., Goodwin,M.J., Cade,L., Zhang,F.,
            Cifuentes,D., Curtin,S.J., Blackburn,J.S., Thibodeau-Beganny,S.,
            Qi,Y., Pierick,C.J., Hoffman,E., Maeder,M.L., Khayter,C., Reyon,D.,
            Dobbs,D., Langenau,D.M., Stupar,R.M., Giraldez,A.J., Voytas,D.F.,
            Peterson,R.T., Yeh,J.R. and Joung,J.K.
  TITLE     Selection-free zinc-finger-nuclease engineering by
            context-dependent assembly (CoDA)
  JOURNAL   Nat Methods 8 (1), 67-69 (2011)
   PUBMED   21151135
REFERENCE   8  (bases 1 to 2986)
  AUTHORS   Liu,W., Guan,Y. and Collodi,P.
  TITLE     A zebrafish cell culture assay for the identification of microRNA
            targets
  JOURNAL   Zebrafish 7 (4), 343-348 (2010)
   PUBMED   21158564
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JBMGRA010000016.1.
            
            On Jun 25, 2025 this sequence version replaced NM_001317223.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: JX448391.1, SRR32588719.5070660.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA3505371,
                                           SAMEA3505372 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-359               JBMGRA010000016.1  55752918-55753276   c
            360-546             JBMGRA010000016.1  55733588-55733774   c
            547-667             JBMGRA010000016.1  55729592-55729712   c
            668-849             JBMGRA010000016.1  55725801-55725982   c
            850-986             JBMGRA010000016.1  55722044-55722180   c
            987-1121            JBMGRA010000016.1  55721810-55721944   c
            1122-1209           JBMGRA010000016.1  55710240-55710327   c
            1210-1357           JBMGRA010000016.1  55708537-55708684   c
            1358-1477           JBMGRA010000016.1  55707437-55707556   c
            1478-1600           JBMGRA010000016.1  55705769-55705891   c
            1601-1734           JBMGRA010000016.1  55704295-55704428   c
            1735-1919           JBMGRA010000016.1  55699882-55700066   c
            1920-2079           JBMGRA010000016.1  55697272-55697431   c
            2080-2170           JBMGRA010000016.1  55695628-55695718   c
            2171-2365           JBMGRA010000016.1  55692498-55692692   c
            2366-2500           JBMGRA010000016.1  55691958-55692092   c
            2501-2602           JBMGRA010000016.1  55691780-55691881   c
            2603-2802           JBMGRA010000016.1  55687835-55688034   c
            2803-2986           JBMGRA010000016.1  55685883-55686066   c
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="16"
                     /map="16"
     gene            1..2986
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="argonaute RISC component 1"
                     /db_xref="GeneID:570775"
                     /db_xref="ZFIN:ZDB-GENE-110606-3"
     exon            1..359
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    308..310
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="upstream in-frame stop codon"
     CDS             335..2911
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="eif2c1; argonaute RISC catalytic component 1"
                     /codon_start=1
                     /product="protein argonaute-1"
                     /protein_id="NP_001304152.2"
                     /db_xref="GeneID:570775"
                     /db_xref="ZFIN:ZDB-GENE-110606-3"
                     /translation="

MEPGPSGAVPVGPYPPPLQQVFQAPRRPGMGTVGKPIKLLANYFEVEIPKMDVFHYEVDIKPDKCPRRVNREVVEYMVQHFKPQLFGDRKPVYDGKKNIYTVLALPIGSEKVDFEVTIPGEGKDRIFKVSIRWLAKVSWRLLQETLVSGRLQVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKTLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNANFNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKSERIGKSGNIPAGTTVDTSITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHVSGQSNGRDPQALAKAVQIHHDSLRTMYFA"

     exon            360..546
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            547..667
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            668..849
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            850..986
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1121
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..1209
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1357
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1477
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..1600
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1734
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1919
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2079
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2080..2170
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2365
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2366..2500
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2501..2602
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2603..2802
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2803..2986
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ctgagggggggtgacggacgggtcgagcgttttgttccgctggtgaatcctgcttgaacggaaagcaggagacacaacaacccgacacacagaagccagaccagccactgacgaaagtgcacggaggagcgggtctatataaataggaactacggagcttttgtttctctctcaactcctgcgggtttgggggctccagcggcggcggcggagaagatcccgctcgcctagggaaggaggctggaagtcggggacgcttccagcctgcagggccaggcgccgtggcgattgagtatctggactttggtgaacccttaggggctcagtggatgggatggagccgggaccatctggcgctgtgcccgtcgggccgtaccctccgcccctgcagcaggtgtttcaggcgccccgcaggcccggcatgggcactgtcggcaaacccatcaaactgctggcgaactacttcgaggtggagatccccaaaatggacgtgtttcactatgaggtggacatcaagcctgataagtgccctcgacgggtcaacagggaggtcgttgaatatatggtccagcatttcaagccgcagctcttcggtgaccgaaagccagtgtatgatgggaagaagaatatctacactgttttagcgttacctataggcagtgaaaaggttgattttgaggtgaccattcctggcgaggggaaggatcgaatctttaaggtgtcgatccgctggttggcgaaggtgtcctggaggctcctgcaggagacgctggtcagtgggcgactgcaggtcccgctggagtccgttcaggctctcgacgtggccatgagacacttggcctccatgaggtacactccagtgggccgctccttcttctcgcctcctgaaggttactaccatcctctaggtggcggcagagaggtgtggttcggcttccatcagtccgttcgaccagccatgtggaagatgatgctcaacattgatgtatctgcaacagccttttataaagctcagcctgtcattgagttcatgtgtgaagtcctggacatacgtaacattgatgagcagcccaagacactaactgactcgcagagggtccggttcaccaaggagatcaaaggcctgaaggtggaggttacgcactgtggtcaaatgaaaaggaagtaccgggtctgtaacgtcacacggcgtcctgctagtcaccaaacgtttcctctacagctggagagcggacagacggtagaatgcacagtagctcaatatttcaagcagaagtacaacctgcagctcaagtaccctcacctgccctgtctacaggtgggacaggagcagaaacacacatacctgcctctggaggtttgcaatattgtagcagggcagcggtgcatcaagaaactgacagataatcagacgtccaccatgatcaaagccacggcgcgctccgcaccagaccgacaggaggagatcagcagactgatgaagaacgctaactttaatctggatccttacattcaagagtttgggattaaggtgaaggacgacatgacggaggtgaccggcagagtccttccagccccaatcctgcagtatggcggtcggaatcgtgccatcgcgactccaaaccagggtgtgtgggacatgaggggaaaacagttctataatggcatcgagatcaaggtttgggccattgcttgcttcgctccgcagaaacagtgtcgggaagaggtgctcaagaacttcaccgaccagctgcgcaagatctcaaaggatgctgggatgccgattcagggtcagccgtgtttctgtaagtacgctcagggggccgacagcgtggagcccatgttcagacacctgaagaacacttactctggactgcagctcatcattgtcatcctgcctgggaaaacccccgtctacgcggaagtgaagcgtgttggagacactcttctaggaatggccactcagtgtgtgcaggtgaagaacgtggtaaaaacctccccgcagacgctctccaacctctgcctcaaaatcaacgtcaaactcggcggcatcaacaacatcctcgtgccgcatcaacggtcagcagtgtttcagcagcccgtgatcttcctcggggcagatgttacacacccgccggctggtgatgggaagaagccatctattactgctgttgttggcagtatggacgctcatcccagcagatactgcgctacagttcgagtccagcggcccagacaggagattattgaagatctgtcctacatggtgagagaattactgatccagttctacaagtccacccgcttcaagcccacgagaatcatcttctacagggacggcgtaccagaaggacagctgccacaaatcctgcactatgagctgttggccatcagagatgcctgcattaagctggagaaagactatcagcccggcatcacttacatagtcgtgcagaaacgccaccatacacgactcttctgcgccgataaatctgaacgaatcggcaagagcgggaacataccagcgggaactacggtcgacaccagcatcacacatccattcgagtttgacttctacctgtgcagtcatgcaggcattcagggcaccagccgaccctcacactactacgtcctgtgggacgacaatcgctttacggctgacgagctgcagattctcacctaccagctgtgccacacgtatgtgcgctgcacccgctccgtctccatccccgcgccagcatactacgccagactcgtggctttccgggcccgataccatctggtggacaaagaacatgacagcggggagggaagtcacgtctccgggcagagcaatggtcgagatcctcaagcgttggccaaagcggtgcagattcaccacgactccctgaggaccatgtattttgcctgaataacaaaaagcgcacgtctccggacacctcgagccagaacccctgggtgctaaaccagtccaatgaagcccaca

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