LOCUS       NM_001135784            2282 bp    mRNA    linear   VRT 23-DEC-2023
DEFINITION  Danio rerio NK6 homeobox 2 (nkx6.2), mRNA.
ACCESSION   NM_001135784 XM_689052
VERSION     NM_001135784.2
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Carril Pardo CA, Massoz L, Dupont MA, Bergemann D, Bourdouxhe J,
            Lavergne A, Tarifeno-Saldivia E, Helker CS, Stainier DY, Peers B,
            Voz MM and Manfroid I.
  TITLE     A delta-cell subpopulation with a pro-beta-cell identity
            contributes to efficient age-independent recovery in a zebrafish
            model of diabetes
  JOURNAL   Elife 11, e67576 (2022)
   PUBMED   35060900
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Mukaigasa K, Sakuma C and Yaginuma H.
  TITLE     The developmental hourglass model is applicable to the spinal cord
            based on single-cell transcriptomes and non-conserved
            cis-regulatory elements
  JOURNAL   Dev Growth Differ 63 (7), 372-391 (2021)
   PUBMED   34473348
REFERENCE   3  (bases 1 to 2282)
  AUTHORS   Yang L, Webb SE, Jin N, Lee HM, Chan TF, Xu G, Chan JC, Miller AL
            and Ma RC.
  TITLE     Investigating the role of dachshund b in the development of the
            pancreatic islet in zebrafish
  JOURNAL   J Diabetes Investig 12 (5), 710-727 (2021)
   PUBMED   33449448
REFERENCE   4  (bases 1 to 2282)
  AUTHORS   Lavergne A, Tarifeno-Saldivia E, Pirson J, Reuter AS, Flasse L,
            Manfroid I, Voz ML and Peers B.
  TITLE     Pancreatic and intestinal endocrine cells in zebrafish share common
            transcriptomic signatures and regulatory programmes
  JOURNAL   BMC Biol 18 (1), 109 (2020)
   PUBMED   32867764
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2282)
  AUTHORS   Seifinejad A, Li S, Mikhail C, Vassalli A, Pradervand S, Arribat Y,
            Pezeshgi Modarres H, Allen B, John RM, Amati F and Tafti M.
  TITLE     Molecular codes and in vitro generation of hypocretin and melanin
            concentrating hormone neurons
  JOURNAL   Proc Natl Acad Sci U S A 116 (34), 17061-17070 (2019)
   PUBMED   31375626
REFERENCE   6  (bases 1 to 2282)
  AUTHORS   Guner B, Ozacar AT, Thomas JE and Karlstrom RO.
  TITLE     Graded hedgehog and fibroblast growth factor signaling
            independently regulate pituitary cell fates and help establish the
            pars distalis and pars intermedia of the zebrafish adenohypophysis
  JOURNAL   Endocrinology 149 (9), 4435-4451 (2008)
   PUBMED   18499750
REFERENCE   7  (bases 1 to 2282)
  AUTHORS   Bonner J, Gribble SL, Veien ES, Nikolaus OB, Weidinger G and Dorsky
            RI.
  TITLE     Proliferation and patterning are mediated independently in the
            dorsal spinal cord downstream of canonical Wnt signaling
  JOURNAL   Dev Biol 313 (1), 398-407 (2008)
   PUBMED   18062957
REFERENCE   8  (bases 1 to 2282)
  AUTHORS   Gribble SL, Nikolaus OB and Dorsky RI.
  TITLE     Regulation and function of Dbx genes in the zebrafish spinal cord
  JOURNAL   Dev Dyn 236 (12), 3472-3483 (2007)
   PUBMED   17994542
REFERENCE   9  (bases 1 to 2282)
  AUTHORS   Hutchinson SA, Cheesman SE, Hale LA, Boone JQ and Eisen JS.
  TITLE     Nkx6 proteins specify one zebrafish primary motoneuron subtype by
            regulating late islet1 expression
  JOURNAL   Development 134 (9), 1671-1677 (2007)
   PUBMED   17376808
  REMARK    GeneRIF: Study shows that zebrafish primary motoneurons express two
            related transcription factors Nkx6.1 and Nkx6.2; in their absence,
            the CaP motoneuron subtype develops normally, whereas the MiP
            motoneuron subtype develops a interneuron-like morphology.
REFERENCE   10 (bases 1 to 2282)
  AUTHORS   Guner B and Karlstrom RO.
  TITLE     Cloning of zebrafish nkx6.2 and a comprehensive analysis of the
            conserved transcriptional response to Hedgehog/Gli signaling in the
            zebrafish neural tube
  JOURNAL   Gene Expr Patterns 7 (5), 596-605 (2007)
   PUBMED   17307034
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CABZ01085047.1 and EH578673.1.
            
            On Aug 10, 2010 this sequence version replaced NM_001135784.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GFIL01028306.1, EH578673.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA3505370, SAMEA3505371
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                CABZ01085047.1     8752-8776
            26-403              EH578673.1         27-404
            404-607             CABZ01085047.1     9155-9358
            608-909             EH578673.1         609-910
            910-2282            CABZ01085047.1     11210-12582
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="13"
                     /map="13"
     gene            1..2282
                     /gene="nkx6.2"
                     /note="NK6 homeobox 2"
                     /db_xref="GeneID:565782"
                     /db_xref="ZFIN:ZDB-GENE-070626-1"
     exon            1..666
                     /gene="nkx6.2"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    6..8
                     /gene="nkx6.2"
                     /note="upstream in-frame stop codon"
     CDS             252..1088
                     /gene="nkx6.2"
                     /note="NK6 transcription factor related, locus 2"
                     /codon_start=1
                     /product="homeobox protein Nkx-6.2"
                     /protein_id="NP_001129256.1"
                     /db_xref="GeneID:565782"
                     /db_xref="ZFIN:ZDB-GENE-070626-1"
                     /translation="

MLAVGQMDSNRQSAFVLGSTPLAALHNMTEMKTSLFPYALQSPAGFKAPSLGNLNSQIPLGTPHGISDILGRPITTAGQLLSGFPRINGLATSAGMYFNPAAVSRYPKPLTELPGRAPIFWPGMMQGSPWRDPRVPCAAQANMMLDKDGKKKHSRPTFSGQQIFALEKTFEQTKYLAGPERARLAYSLGMTESQVKVWFQNRRTKWRKRHAAEMATAKKKHDSETEKMKESSDNDEDDEYNKPLDPNSDDEKITRLLKKHKTSNLSLLSPCSNSSDTL"

     misc_feature    699..875
                     /gene="nkx6.2"
                     /note="Region: Homeodomain; pfam00046"
                     /db_xref="CDD:459649"
     exon            667..839
                     /gene="nkx6.2"
                     /inference="alignment:Splign:2.1.0"
     exon            840..2282
                     /gene="nkx6.2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

cgcattgaccggggaatcgcacctcagatgcggggaacacgctgcactcgaagcggccgtcagatctgccgaacaccgccgattggattgcgctccggaaactcactcgcgcgtgggaaacatttgcaaagtttcgcctccagttgaaagttcagttggatgagctgtcaaactttatttgctgtactttgtgtgtgctgtgttcttggtttttgtttctccgcggtttgtcctgtggctccggcgcaccaatgttagcggtgggacagatggatagtaaccggcagagtgcgttcgtcctgggcagcactccactggctgcgctgcacaacatgaccgagatgaagacctctttattcccgtacgctctgcagagtcccgccggcttcaaggctccttctctcggcaacctgaactctcagatcccgctggggacgcctcatggaataagcgatattttgggcagacccatcaccactgcaggacagctgctctcgggtttccctaggatcaacggattggccacttcagccgggatgtacttcaacccggcggccgtttctagataccccaaaccgctgacggagctgcccgggcgggcgcccatattctggccgggaatgatgcaaggctcgccgtggagagacccgcgggttccctgtgcggcccaagcaaacatgatgctggacaaagatggcaagaagaaacattccagaccgactttttcaggacagcagattttcgcactggagaaaaccttcgagcagaccaaatatctggccggtccggagagagccagactcgcctattctctgggaatgaccgagagccaagtcaaggtttggtttcagaacagaagaactaaatggcgaaagaggcacgcggcagaaatggccacggccaagaaaaagcacgactctgagacggagaagatgaaggagagctcggataacgacgaagatgatgaatataacaaacccctggacccgaactctgacgacgagaaaatcacaagacttttgaagaagcacaagacgagtaacctgtcgctcctcagcccgtgcagcaacagctcggacactttgtgatcaatctgagctcaaagactcttcatgcactgaaaccaaaacatgatgcattggatttactacatgtgtttaaaggtaactggctgcaagcaattaatatgggctgaatgttagcaagttgaacattactgcatttaatttgtgtttaaattcagccgacacactctctatttcaacacaaatccattaagctaatcgttttttacaaatttgagtggattgaacataaaacaattaagttatcccaaaacaaaagtaaagaattgtgtttcttcaactcattttaatttagtttaatcacacagaagtcatcttttgagtgcatataaattgtttgcagccagttaccttaaattcttcagtaaattcaataactcattcccttttcagtgcatcttcagcatcatcttcatcagtctcagattctcctgcagaactggggcagaattaagcagggtataataaacacactgttcacactgctttggcttcgctgcagttgtacagttagagtggacgtgagatgtatatatattttttactgagtaaattatgttattaaagcatcacgaagacgtgccaatctcccctcatcatcatcataggtcatttaagatcagacggtcgcactttacaacaaggctgtactagttaatatcagttaatgcatttattagcatgaacgaacaggacattcatggcagtatttattcatgttaatacagtgttcactgttagttcgttaactcacggtgcatgaatttggatgttaataatgcatacatgttgaactatcattaagaaattatgtacatgtattgttgattaataattcatgtagtaaatacattaccaatgcattaactaatgcaacctgattgtaaagcgtgaccaaaatcgatcgcagaagaataacgacgcctctttcgcctaaaaacatgtacttttttattattttgaaagcaggtacacaaagtgtataaagtgccagtgttgtaaatgatgtgaatagagtttgttttaataacaatgtcctttttttggccgcttatactttcaatggaaggagtttatgttttacctcacaacacgagaaatactcaaagatggacttaaatatcgatgaagagaatattattgcttccgtgatgaaggaagaaaaactttgtattatcaataaattatttaacaagc

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