LOCUS       XM_067612376            8999 bp    mRNA    linear   VRT 22-AUG-2024
DEFINITION  PREDICTED: Thunnus thynnus argonaute RISC catalytic component 3b
            (ago3b), transcript variant X2, mRNA.
ACCESSION   XM_067612376
VERSION     XM_067612376.1
DBLINK      BioProject: PRJNA1130912
KEYWORDS    RefSeq.
SOURCE      Thunnus thynnus (Atlantic bluefin tuna)
  ORGANISM  Thunnus thynnus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Pelagiaria; Scombriformes; Scombridae; Thunnus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_089531) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_963924715.1-RS_2024_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/03/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Thunnus thynnus"
                     /mol_type="mRNA"
                     /db_xref="taxon:8237"
                     /chromosome="15"
     gene            1..8999
                     /gene="ago3b"
                     /note="argonaute RISC catalytic component 3b; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 57 Proteins"
                     /db_xref="GeneID:137198498"
     CDS             1202..3784
                     /gene="ago3b"
                     /codon_start=1
                     /product="protein argonaute-3 isoform X2"
                     /protein_id="XP_067468477.1"
                     /db_xref="GeneID:137198498"
                     /translation="

MEIGTTGAAEARALFSLPRRPGYGTIGKAIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDHLPVYDGKKSLYTASPLPVATSGVDLDVTLPGEGGKDRPFKVSMRFVSLVSWHMLHEVLTGRTMPEPLDLEKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSSPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPATLQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYEADPFVQEFQFRVRDEMAQVMGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPIIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEVIQDLASMVRELLIQFYKSTRYKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRNERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPTALAKAVQIHHDTLRTMYFA"

     misc_feature    1424..1702
                     /gene="ago3b"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    1730..1882
                     /gene="ago3b"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1883..2245
                     /gene="ago3b"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(2039..2041,2084..2086,2126..2128,2138..2140,
                     2192..2194,2213..2215,2219..2221)
                     /gene="ago3b"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    2378..3655
                     /gene="ago3b"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2789..2791,2801..2803,2837..2848,2855..2857,
                     2879..2881,2888..2890,2900..2902,2912..2914)
                     /gene="ago3b"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2993..2995,2999..3001,3209..3211,3623..3625)
                     /gene="ago3b"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      8999
                     /gene="ago3b"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

ttcattacttgacctatataacatatacatatatatctatagctaccagaaccactgcttctgcacatatttggcacaacttttctatttttacaacccacaagttagaaaatgtaacctaagtaatgtaatgtgtataatgtttataacgtatataaactgaattctttattactcctagttcttatctgttaccttgtttcctgcactgctgctgtaacgtgaatttccccctctagggatcaattatcactggtgtgtgacagcgagaggggaagaaactgttcccgtgtcggtggttttggtgcacaacttattaattttacagccacacttccaatcctctcatcaaactgaacagatgctgtattccaaatgttagactcatcttcaaagtctcatgccaatcaaaaatgtgtttatgcccactgttacttcacttggatgcttaagcttcaccgtgcagaatgatacaatatttacatatttattgattatggatttttaatgagagagaagggagattttaacatagtatctatacttctttgtgaaaaaacaaaagcaatcatatcagacacacatcatggttccaagcagagtattttatatgtcttgacacctgtctgcaacaaaacagacatctcctgcgcaacacctgttgccattaatcctactttattttgaaagagcgttgcggaagtagaaacatgctaatgctactagcttgccgttactaagagagagggcggcgtaacagcagatttcagaccggtttctcaccgataccgaggaggacgccggcgtagtgaaccccacatctcagtttgataaagaagaacaccgaattgtcacgttaaccaaccgggaaaacgaatccagtcactgaacggccacaacacaaacaactttggaagtacttaagcggggacgttttgtacaactttgagctgccaagttgccggtgaggcaggtcggggagagaggctccaggcaggtggaaaaccacaagcggcccggttcaacatcagcgaccgaagccggggagcagagagccggggagccgtaagggggagcggggtgcccggggggaagcggggagccagcggggagcggcgtgtcggccagcaaagctttaacctgcgctgggagaatcgatcactgggtgccggccgggttcatccggactgagacctcatgaatggaaatcggaacaacaggagccgctgaagcccgagcccttttttcgttgccacggcgacctggctatggcaccattgggaaggccatcaagcttctggcgaactgcttccaggtggaaatccccaagatcgacgtctacttgtatgaagtggacatcaagcctgataaatgtcctcgccgggtcaacagggaggtggtggactccatggttcagcatttcaaggtgaccatctttggtgaccatctgccagtttacgatgggaagaaaagcctctacactgcgagcccacttcctgtcgccactagcggggttgatctggatgtcacattgccaggtgaaggtgggaaggaccgcccgtttaaagtcagcatgaggttcgtgtcgttggtcagctggcacatgctgcacgaagtcttgacgggacgcaccatgcctgaaccgctggacctggagaaacccatcagcactaaccccgttcacgccgtggacgtcgtccttcgacacctgccctccatgaagtacacccccgtcggacgatccttcttctcctccccagagggctacgaccacccgctaggtggaggaagagaggtttggtttggttttcatcagtcggtacggccggccatgtggaagatgatgctcaacatcgatgtgtcagccacggctttttataaagcccagccagtcatccagttcatgtgtgaggtcctggatattcacaacattgacgagcagccccgcccgctcacagactcccacagggttaagttcaccaaagagatcaaaggtcttaaagtggaagtgacacactgcggaaccatgcgtaggaagtacagagtctgcaatgtaacacgccgccccgccaccctccagacatttcctctgcagcttgagaatggtcagacagttgagcgcacagtggcgcagtacttccgagagaagtataatctgcagctgaagtatccccacctgccctgtctgcaggtgggccaggaacagaaacacacctacctgcccctggaggtatgcaacatcgttgccggacagcgctgcattaaaaaattaacagataaccagacgtcaaccatgataaaagcaacagcgcgttctgcccccgacagacaggaagagatcagcaggctggtgcggagcgcaaattacgaggccgacccgtttgtccaggagttccagttccgtgttcgtgacgagatggctcaggtgatgggccgcgtcctgccggcccccatgctgcaatatggcggcaggaaccgcacggtggccacacccagccacggggtgtgggacatgagggggaagcagttccacaccggagtggagattaagatgtgggccatcgcctgctttgccacccagagacagtgccgtgaggagattctcaagagcttcactgaccagctgcggaaaatctcaaaggatgctgggatgccaatccaaggccagccatgtttctgtaaatacgcccagggagctgatagtgtggagcccatgttcagacacctgaagaacacctacgctgggctgcagctaatcatcgtcatcctgcctggcaaaacgcccgtctatgctgaggtgaagcgggtgggagacaccctcctgggcatggccacccagtgcgtgcaggtgaagaacgtagtgaagacgtccccacagaccctctccaacctctgcctcaagatcaacgtcaagctgggaggcatcaataacatcctggttccacaccaacggccctctgtgttccagcagccgatcatcttcctcggggctgatgtcactcatcctccagcaggagacgggaaaaaaccatctattgcagcggtggtgggcagcatggacgcccatcccagcaggtactgtgctacggtgcgggtccagaggcccagacaggaggtcatccaggacttggcctccatggtgcgagagctcttgatccagttctacaaatcgacccgctacaagccaaccaggatcattttctacagggacggagtgtctgagggccagttcagacaggtgctgtactatgagctgctggccatcagggaggcttgcatcagtctagagaaggattaccagccagggattacctacatcgtcgtacagaaacgccatcacacacgcctcttctgtgcagatcgcaacgaacgggttggaagaagtgggaacattcctgccggcaccacagtggacacggacattacccacccctacgagtttgacttttacctctgcagtcacgctggaatccagggtacgagccggccttcccactaccacgtactgtgggacgacaactgtttcaccgccgacgagttccagctcctcacctaccagctgtgccacacctacgtacgctgcacgcgttccgtctccatcccagcacccgcctactacgcccacctggtggccttccgcgcccgctaccacctggtcgacaaagagcacgacagcgctgaaggcagccacgtctcagggcagagtaacggcagggaccccacggcgctggccaaggccgttcagatccaccacgacacactgaggaccatgtacttcgcctgatcgcccaccccccccccacctccacttccctccaccttcaccagcccactcagccagtcttagtgttcgacttcggatcagccgcctcgtctcatcgcgcagagcgacgtcgtgccgtcgcagctttggtcaccggaaaacacgacgacggcagcaggctgcgtaaggatgtcataagccagagaggagtacagctgcattatgcaatttgaaaccagccaattgtttctgtgctcccacccatgcatctctctttttttctccccctcatctcctctgctgtattttaaatcatgatggattctgtctcctttttaaaaactcacatcctcgccttccacattttacagcagctcaaagttttgttttgttttgttttttttttaatttcaagtgtgtgcttctggatcataaaacagagcatggcgaggtagcagggaatacaaaaacagcagatttttttttgttttgttttttttttgttgttgcatgggtgggcacgaagcagcaccagtgaggacccgacttgagcctcacgttgagcctaatgtcgctggtttatattgtagacggttttacatgtttgtgtgttttcagcttgtctagatacaatactgtatttgtctgtcgagcaaatgtgtgattgccatggcaacgtgttagtcgagaacactcgtaaatagcaaagcttctcgccccccccccttttttttttttctttctctctctcaaaaacggcaggctgccttactccttactcttctgtctccctttaagccaatggatggttattcacagtcattcagccagacagtaagaagcgtatctctacactcttaccagcacagacactgaatgaaacctttttttttttttttaaacaaagccacgtacattgccattcttttgaggcattgtgtgtatcgcttgttgccttactgcgaggcgaggtgcataaaaaggactgttagaatacgacgccatggccacaaaatggacaattttaaaaactctcactttaatagctcagcttggtacagaataatataccaaacgtttctcatgtgttttattcgtcttgctatttatcagttttcgtctctttttaacttaaaataacatttattttcagcaatagagcagtttttatcttctgatgaccacaaaccttttttttttttttttttcccccacacttttaacaaaaaaactgaccaatttagtcgtcaacagctcgttctgtgcaataaggtgttaaagcagagagaacaggttcggtggtgattgttttcgatgagtatttgtgcgctaaaacggttctcgagcgagttttagcaagtgataatgaactcggagtaggattgacgccgcagatgtgacgcgtggatgagtcgtctgacggcgtttttgtgagattataacaaaagtgcctgatttggttagagtgtgaaatgttggcaggacggagggttttttaatcgtatgttggtttgggtgcctctgttaatgcatttagtttaacagtaactataaaaaaaacactgcactgcccgacatgaggggctggacagcacagtcacatcacaggccgggtttgtgtgattatttctaatgttttctcattataaggaagcagttatttcctgattcccacttttaacgcactttgtttcctcgtgaacacaaaatattttgttcttggaatgaaatggagatcggacggcggtttaatcattggccaaaacagtttcggtttcagataaaattaatgaagctaaacaaaaattgcaatttcggccaaaactttcttgtcatatgatttaaaaatctttaaaatgtcaaaatattctccaggtaacaatctcgttttcttgatcactaagtaattatcacaatgaaagtttatttaaaataatgtcggactttgttttttcatgataattagtaatcttgtcatgatctcaaaaaacaaagcgagattttaaagtcatttttgtgtttgcgagaaaactaaaacaacataaataatcacacacagttggcgtcttcggccttccgtacgatgctgtaataaatatctgaatatctgaactatataacatgcagctaagtgaaggcagagactgtgtacttggatgtgcgtcctcacatgatgcaccgtcaggactcgcagtttgcactcttcagtcgaagccttcgtattcagatttcagagtctctgtcgcgttagttttcagagtcggtatgagctgggtgtcactcgactggcagtattatagcaccaaaaccgctttcagtactcgaaactcaacacggttccaggatggtgctcgatttcataaaacctttctattttggatcaaataatgtatcattatgggtaacgcttcctaattttatactgatttcctggtcattttttgcaggtatttaacagttaatttttaggacgttttgttcaaattataagataaaaatgtaaaaaggtgttaagttggtaaataccggttcattataggctatttgtatacgtagatgttctttttcaaaatttcttaataaaatagacttttgacagtttttaactgacagaaaatacttggttgtcagtgtgtagttttatgtgctaaacccaacattaacatattaggtttttttcaataatttcctaaaagtagctgctgtgtaactaataataataataataataatcataattcttaggacatttctttgaaagggttatgagggttaatatgctaatatatagtttgacacaaaaaactacacactgaaaactaaatatcttctgtcagttaaagaaaaattaccaactaaaccaatttaaacaaagtttcctgtaatttttaccaaattgcacaacagtctctgtaaaatgtcctaaaaattaaccgttaaatacctcaaattactctaaaaatttccaaaaaaattggtttaaaattaaaggacctgtaaaataaagcgttaccgcattatgcaaataaagcagatttttctctgctacttttatacacacttctgatttaccaatatggagttaaatgcaaaaattaatcgatataatcaagtaaaaagaaatctgtttataagaatccaacattttgaagtttatctcgttgccttttaatctgtcatcagaacttgggtcacagtgctgccggtttctggacgcccagccctgcagagtttagtccgtcatggtctcgcagtgtcgtttaacacggacggtttcatcactttggaccaaaccaacgtgcctggttgagggtgagcctggagggttggatggtctctgtactgctaactgtgctcgccacacatcaccagaaccaaacttcagctgctataagaaaacatttatttaaatatttcatcagatacatatatataggctatatatattaatacagagagacttagaggttatggtattttactgcacttaaaaagaaaaatagaaaaaaaaagatgcatgtgtttgtggagtcgagatgctgagatgtgcgttggtttagactctttctttgcctgcagttaaaacatttttaacaataatccaagcgccctttttgtgtttgtcgtcacgttgaagctgtttttttttttttttttttaatgtcagacattaccagcatttgatttttacttgaaaaagagtcagttgtgggcatgtttaacattgttggcgagtgctctgcttgttttttttttttttttgttttattttgaaagtgtatattgagtcttttccactcacgacgccacgtacagtaaatcgacctgaagacgcctgtaggcagggcgcagggccagacatcagatgcctttttttttttttttttcttcttcatgtgatttgaagaaagagaagaaaaaaaaaaaagaagcatttttaagtctgaacgaagatatatttatatttaaagatattaatgtaatgcacacactacttattttttctaacagaatcctatttgatgcggaatcgtaacagctgtattgaaatatcgacgttttaataatgatcgtttttttccaaaaaaaatttgataatgcaatacttcacgtgaaaagcgaaaccgatgctgcattgacctgctttttgtggagacggtcggagggtttgttctgtcatgtagtgatgagatgaaccgtcaaccatcctctgcttcttcttcttcttcttcttcttcttttttttaaaattcaccaaactaaaggcgagctgtgaagacctccctctactctgaattttattttatcatctcaaaagctcatgaaggaagcagaagactctaatggggtttaacacatttcttaatatttttacagcactgcaaactgcatcattaaaggttttattttgggtctttttcttctttttttttttttttttttttttttttttttttttttgcatattcacaagaggagggttgaaagttcatcctcagacttttgtcgattttgtccgttacagactgtgcctttcctgatggtgctgaacagaaaacaaacaaacaaacaaacaaacaaaaaaaaaaactgagtaaaacaatgaaatcttaacgttcagaacgggcgccgagctccgagtctacatggataagtatatattgatacatgacaatactgtaagttgtgattgtgtgtgtatgtttggtttgtgagtgtgtgtgtgtgtgtgtgtgtgtgtgtgagagagcgcgcgcgtccaacatatgccaagaacagataatatgcagaactgatcagttttcggagctgacaggaccaaacggcctgtgggggatcggttttaagtggacgggcactctggagcacaacccgaaacatttcttcttcttcttcttcttctacttcaaaaaaaacaaacaaactcatgaaaacgaggatctaaacattatgtagcattatttatttgacttcatctctacgggggacacgacgcgtctctttactgcctgttaacaacattttttttttcattatttactgtcaatttcatgtgttcattcttaaatggacggcaataatgaagtgtttgtgcaatcggtttttcctttttaattgtagtttttcttcttttttttgtttctttttctttttttttcttctaatgtgaaattttgggatagtttggttgtgtcatcatgagtgcccgtttccccacaaacataaatctttacac

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