LOCUS       XM_067496220            6530 bp    mRNA    linear   VRT 20-AUG-2024
DEFINITION  PREDICTED: Channa argus argonaute RISC component 4 (ago4),
            transcript variant X9, mRNA.
ACCESSION   XM_067496220
VERSION     XM_067496220.1
DBLINK      BioProject: PRJNA1148499
KEYWORDS    RefSeq.
SOURCE      Channa argus (northern snakehead)
  ORGANISM  Channa argus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Anabantaria; Anabantiformes; Channoidei;
            Channidae; Channa.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_090197) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_033026475.1-RS_2024_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/16/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Channa argus"
                     /mol_type="mRNA"
                     /isolate="prfri"
                     /db_xref="taxon:215402"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="muscle"
                     /dev_stage="adult"
                     /geo_loc_name="China: Weishanhu"
                     /collection_date="2021-12-20"
     gene            1..6530
                     /gene="ago4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 3
                     Proteins"
                     /db_xref="GeneID:137122831"
     CDS             156..2588
                     /gene="ago4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X9"
                     /protein_id="XP_067352321.1"
                     /db_xref="GeneID:137122831"
                     /translation="

MVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVSVVSLQMLLEALSGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRVSTDTGRDCGRGLSPQNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDMSQEQLFSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHYDTQHTMYFA"

     misc_feature    165..419
                     /gene="ago4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    450..602
                     /gene="ago4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    603..965
                     /gene="ago4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(759..761,804..806,846..848,858..860,912..914,
                     933..935,939..941)
                     /gene="ago4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1104..2459
                     /gene="ago4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1563..1565,1575..1577,1611..1622,1629..1631,
                     1653..1655,1662..1664,1674..1676,1686..1688)
                     /gene="ago4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1767..1769,1773..1775,2013..2015,2427..2429)
                     /gene="ago4"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      6530
                     /gene="ago4"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

gcacggtgggtaaacccatccggctccttgccaaccacttccaggttcagattcccaagattgatgtctatcactatgatatcgacatcaaacctgagaaacggcctcggagggtcaacaggtaaatcatcaccacaagggaggtggtggacacgatggtgcggcacttcaagatgcagatctttggagacagacagcctggatatgatgggaagagaaacatgtacacggcacatccactgccaatagggagagaccgggtggacctggaggtgactctgccaggcgaggggaaggatcagacgttcaaggtgtctttgcagtgggtgtctgtggtcagtcttcagatgctactagaagccctgtcgggtcatcttaacgaggtcccggaagactccgttcaggctctggatgtcatcacacggcatctgccttccatgaggtacactccagtggggcgttcgtttttctcccctccggagggctattatcatcctcttggtggaggcagagaggtgtggtttggtttccatcagtctgtccgtcctgccatgtggaacatgatgctcaacatcgatgtgtcagccactgctttctaccgtgctcagcctgtgatagagttcatgtgtgaggtgctcgatatacagaacatcaacgaacagaccaaaccactgactgactcgcagcgtgtcaaattcaccaaggaaataagaggcttgaaagttgaggtaacacactgtggacagatgaagaggaagtatcgtgtatgtaacgtcacacgccgacctgccagccaccaaacgtttcccttgcagcttgagaatggccaagccatggagtgcacagtagctcaatatttcaagcagaagtacaatctgcagctcaaatatccacatttaccctgtctgcaagtagggcaagaacagaagcacacctatcttccccttgaggtctgtaacatagtagcaggccagcgctgtatcaagaaactgacggataaccaaacgtccaccatgatcaaagccacagctcgctcagcccccgacagacaggaagagatcagcagactggtcaaaagcaacagcatggtcgggggcccggacccttatctgaaggaatttggcattgtcgtgcacaatgacatgacagaagtaacagggcgtgtcctcccagcgcccatgctgcagtatgggggccgggtgagtacagacacagggcgggactgtggcaggggactctctccgcagaataaaaccgtggccacgcccaaccagggcgtgtgggacatgagagggaaacagttctatgccggcattgagatcaaggtctgggctgtggcctgctttgccccacagaaacagtgccgtgaggacctgctcaagagcttcactgatcagctgcgaaagatctccaaggatgctgggatgcccattcagggccagccatgtttctgtaaatacgcccaaggagctgacagtgtggagcccatgttcaaacacctcaaaatgtcttatgtaggactgcagcttattgtggtcatcctgcctggcaaaactccagtttatgctgaggtgaagcgggtaggtgacacccttctgggcatggccacccagtgtgtgcaggtgaagaatgtagtgaagacatccccacagactctctccaacctctgcctcaagatcaacgccaagctgggaggcatcaacaatgtcttagtgcctcatcagaggccttccgtgttccagcagccagtcattttcctgggtgctgatgtaacacatcctccagcaggggatgggaagaagccatccatagcggcggtggtgggcagcatggatggccaccccagccgatactgtgccacagtgcgagtccagacatctcgacaagacatgtcccaggagcagctctttagccaggaagtaatccaagacttgaccaacatggtgcgagagctgctcattcagttctacaaatccacccgtttcaagcccacacgcatcatctattaccgtgggggcgtgtctgagggacagatgaaacaggttgcatggccggagctgatagccatccggaaggcatgtatcagtctggaggaggattacaggccgggtattacatacatcgtggtccagaagcgtcaccacactcggctcttctgctctgataaagctgagagggttggcaagagtggcaatgtccccgcaggaaccacagtggatagcaccatcacacacccgtccgagtttgacttctacctgtgcagccatgctgggattcagggaaccagtcgtccctcccattaccacgtcctgtgggatgacaattgtttcacagccgatgaactgcagcttctcacctaccagctttgccacacctatgtgcgctgcacacgctctgtttccatcccagcgccggcttactacgccaggctagtggctttccgagcccgctaccacctggtggacaaagaccacgacagtgctgaaggcagccacgtttcgggccagagtaacggccgggacccgcaggcgttggccaaggcagttcagatccactatgatacccagcacaccatgtacttcgcctgagctagcaagcacatcagccagtccataagcggattccctttctttctgtctttttagctcttatttgccagtctctccctccttgtcccatttccttccttgtagtctgtctctttctcttcatagtcactctctacattttctgaatctgcaccaaagcggctcttggacaagagaacctgctgggtcccaattttcagcaggctctgttttatggaacaatctccaaccagcgttcccaatcaagccgcctttctaaccagcaatccagcactgtgtgaaccctgagggttctcggtgcacaaaaggaaaaagaacacgaaaaaacaaaaacaaaccatacacacatactacaagttgagccattattttgagtttgtttttgttttttctatttgaatgtctgcactcttgtgttttaagatacactgagcgagggagtggattggcacaatccatgtcagcgcagctctttagctcttccagctaagcaggactcttatctacttttacctcaaagcccctttgggcaaaatctgtcacaaggtcttgggtttattgggtggtggggagggaactctatgtgaaaagagatggatgggagtcgaccttttgagaataattatatattttcctttttatgagcgtgtgttttaaccttttctcctttacagtcttttacaagcgaggtgacctgtgtgtccatgcccattattaccccccatctttgctgtgcgtgcgtgtttgtttgtttgtttgtttgtgtgtgtgtgcgtgtgtgtaagaaagagaatctctgtgtatatacatgtgtgtctgtgtgtttcatggcaggccactgaattgtaaagggaaatcaaggagataactgctgtaaatgcctcagatttgtttttatattcacccatacagtacatataagcatatatttacatctatagagcaactttgagacatatgacttaaacaggcatcagaggctggtgtgggtatttgtagcggtcctgggtcacttactgattgaaatgctttgatttacattttgaacgcgtgcattagtccggtcatgccactggatcagcgggattggcaccttttggaagccagctgctcctcggtgcctaaaaacaccattgaatcccaggaaaaaagtaagttaatccacttcaagtagtaagtgaaccctggtctgaagtgtagcagtagtaacctgtggtctgggctgtgactggctcagcattgggacctgttgaatggctgaaggcattaaacacagtatggtcttatcggtctttctgtgtggaaaaggggatcttatttttctttggacctgattacctgaaagatgtgtgatacagctgggtgttgttgtttttttcctttttttttttttttttttttttttaaagtagtttaaaggccggaaatcaacagtttttaggtctcctttgccaatactggccaactcattgcactgacccgggatgctaggcgagcaacagagggcactctcaatctcttacctttatgcatttatacatacgaatcaacaaagcagatttgtaagtttaatataagatgttttgtggatatcttaaaaatttagaattagtagtttaccattgatgttgttatcgttgtcgtcgatgttgttactattgtgatgaaattttaaggttggcagcaaagccagatatattgttactagtgtgacttcaggaatagacttcagttgctgagctgcaggttgaggggcagagcattgaattggaacttttgtagcattctggctaaagatgaatacaagtaggccccaggatggatgcatccatgcttcctcagtaggttctgattgatggaatgattggcagcaggttggagacattgtgtagcactcctactggttaaacagatctgctgctgtgttaactaggctggtgttggatcgatggagacaatcagggattgaaatcagagcgaacttaagtgagggatttttaaaaccggtttgcgataagcaatgagttgaaattttagactttttaaaatggtttctattactgtgtatttgaagtatacccatcttaaagtctactatttaacggtgtttgtttcatatttttatgcatttttaacactggattaaatgaattgacattgagcataagcactgcccttggacgcacatacattcagcaaaaacggtaccatggaaactgtagtggcatcagtgatggtgacagttgcacaagtggtgccccctggtgttgtggggcaaatgtgggacggaggggtttgtagaaacagtcgacgttcacgcctcattggggggggacagctcagtgaaaacccttttgtaataaaccacccgtaaagcagcaggtttactatgaatgtgtttgagcacctgcctattgaaggcgtgggcgttggcatggtctgagagtagagccccaggaaatgagaagggggggggggggggggggatgagggaaggtgttgtccaaggattgcatgcaataagttttactctgattaaagggggcagatcagtttttaaaacgtgcactagtttaaccaagccaagtgggctgtgattgaatctctaatggagatgggttgaattttaaattacaaagcttgaccattaaactcgaggccgacattggatcactccctgtttataacacattgcaaagcaatattaaaccagttccagttttcagatgtggataaacaaagctataattctactcattgtggttgttggtctactcgctattgtgtctgtgtacttttatttgtttgtgttttattttaatttttttttttggttcattttatttgcttgtatcaatcagtgggtatgtgcatttggtgaaatagttatgtaggctttagtcaatttccttttaaagtaaaaaaaaaaaaactgtaaactgctcgcctgcatgccagattattgtccttttttaaaaaaaaaaatccttttggaatcatatttatgagagaaaaaaataattgcttttctttttgttaaatgtttatcaatttagtgggactgttttaggctagcaatatttgttcagtctctcaaagatatattttaaaaattcctacacaaatgtcagtaatggggggggggggttacaaacgctctcctgaggtgaggggttctggttctttgcgtcgcgttcaaattcaaacgaggtactttcttcaaacgtggacccgttaaagaccgaaacccctctcttctttgccaggtaactgtagcgagcatccgtttttgtctcctcgttcctggttcgtaccagtgtccacgccttttggtggaggctgtcctgtctccatggcaactctgctgcccctcacaaatgcacttcctctttgtgaaggggtccctatggcatcaaagagtgggattacctctttttaacctttttgaaaaacgagacccagaattctttctgtgccccaatagaaagagagcatgaattatttttaaattcctctcactttgatatattgttcttaacagatttgtaatttgacattgtgtattgtgtatcttaatttaattttcttcttttggttcttttttttggggggggggggggttgttttagctctatttttttttcttttttttctgctagtggtctctgaagctgtgttgaaatgtttaatagcaatagaatcatagaattggaacgttccaattctgtgaatggaacgttgtgatcctatgaatgaatggaacccttattgaccagttctgagcgtggggcatgatcaaaccccgcccccagcagaaggccattcatttgctgttattgctattatgtgacatgtttaattttttttcttttctttagcctgcagggaattcttgtgttaatgtgcaaagtaataaattggtattttatgcctataatctaa

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