LOCUS       XM_067447818            8934 bp    mRNA    linear   VRT 19-AUG-2024
DEFINITION  PREDICTED: Pseudorasbora parva argonaute RISC catalytic component
            3a (ago3a), transcript variant X4, mRNA.
ACCESSION   XM_067447818
VERSION     XM_067447818.1
DBLINK      BioProject: PRJNA1148495
KEYWORDS    RefSeq.
SOURCE      Pseudorasbora parva (stone moroko)
  ORGANISM  Pseudorasbora parva
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Gobionidae; Sarcocheilichthyinae; Pseudorasbora.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_090178) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_024679245.1-RS_2024_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/16/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Pseudorasbora parva"
                     /mol_type="mRNA"
                     /isolate="DD20220531a"
                     /db_xref="taxon:51549"
                     /chromosome="7"
                     /tissue_type="liver"
     gene            1..8934
                     /gene="ago3a"
                     /note="argonaute RISC catalytic component 3a; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 18 Proteins"
                     /db_xref="GeneID:137082562"
     CDS             828..3431
                     /gene="ago3a"
                     /codon_start=1
                     /product="protein argonaute-3 isoform X4"
                     /protein_id="XP_067303919.1"
                     /db_xref="GeneID:137082562"
                     /translation="

MEIGTTGAVGAQSLFSVPRRPGYGTMGKPIKLLANCFQVEIPKMDVYLYEVDIKPEKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKKSLYTANPLPVAPAGVDLDVTLPGEGGKDRPFKVSIKFVSLVSWHLLHEVLTGRSMSEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYEADPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRVSTEHFMNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEVIQDLASMVRELLIQFYKSTRYKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKEYQPGITYIVVQKRHHTRLFCADRNERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTLRTMYFA"

     misc_feature    1044..1328
                     /gene="ago3a"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    1356..1508
                     /gene="ago3a"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1509..1871
                     /gene="ago3a"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1665..1667,1710..1712,1752..1754,1764..1766,
                     1818..1820,1839..1841,1845..1847)
                     /gene="ago3a"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    2004..3302
                     /gene="ago3a"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2436..2438,2448..2450,2484..2495,2502..2504,
                     2526..2528,2535..2537,2547..2549,2559..2561)
                     /gene="ago3a"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2640..2642,2646..2648,2856..2858,3270..3272)
                     /gene="ago3a"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

acaagctaatgataatgatggttatgatgatgacggtcactcgtggactcgcgcttcccctcgcagccaatcagcgcgtggctctcgcgccaggtccgtgcgtcgcgctcgcggaggatcagacgctccatatttagctgtagcgagtctcgcagaggctccgactgtcagagaaaccctgagagagagttcaactctcaacttatttcctgtcgccactggcttaaaacgaagggactaagcgctattgaaaagccactggcttcgagtgtggaattcagtgatgatcggggactttaagtgcagagaagactgcgtttgacgggatttttgccccctctcagatgggaatgtagcttagctggttacgttagcccggccactagcagtagcaggctagcgctgtcgaagagaggaggcgacataagtggactttcactcgctataacgtcataacacctcaacaaaccgcctcactctttccaaagacgcgtttgtgaaggcgtcgccgtcacagaaacagcgtttcgggactgtattggggcaggctgaggggaacttttccgtttagctgtcttagctagctggccgtcacgtcaatctgtcagccgaacctgaaatcccgaccagatagttcgagccagataccggggcaccgagcctcccaccgttgatatacgagcttctgggacggtggattaggagcccgcaacacgggaggagacgcttgctttgggttaacgggttagcggccgtcgggatccgagttgagtttctgaagttcgtctactgcgagccggggacggaaccggacagagacctcatgaatggaaatcggaactacaggagccgttggggcccagtccctgttctccgtgccacggaggcccggctatggcaccatggggaaacccatcaagctgctggccaactgcttccaggtggagatccccaagatggatgtctacctgtatgaggtcgacatcaagcccgaaaagtgtccacgccgagtgaacagggaggttgtggactcaatggtgcagcactttaaagtcaccatctttggggatcgaaggccagtttatgatgggaagaaaagtctctacaccgccaacccactgcctgtggcgcccgctggggtggatctggatgtcacgctgccaggggaagggggcaaagaccgaccctttaaagtcagtattaagtttgtgtccctggtgagctggcacctgttgcacgaggtactaactggtcgcagtatgtcagagcccttggagctggacaagccaatcagcaccaacccagttcacgctgtggatgtggttctacgccatctaccctccatgaagtacacaccagtgggccgctcattcttttctgctccggagggctacgaccatcctctgggtggaggcagggaagtgtggttcggcttccaccagtctgtccggccagccatgtggaagatgatgctcaacattgacgtgtcagccacggctttctacaaagcacagccagttattcagttcatgtgtgaggtactggacatccacaacatcgatgagcagcctcgccccctcacagactcccacagagtcaaattcaccaaagaaatcaaaggtctgaaggtggaggtgacacactgcggaaccatgcggaggaagtaccgggtctgcaatgtgacccgccgccctgccagccatcaaacgttccctttgcagttggagaatggccaaactgtagaacgaacagtagctcagtatttcagagaaaagtacaacctgcagctgaaatacccccacctgccctgtctacaggtggggcaggagcagaagcacacctaccttcccctggaggtgtgcaacattgtagcagggcagcggtgcatcaagaaactgacagataatcaaacatccaccatgatcaaagccacagcacgatcagcaccagacagacaggaggagatcagcagactggtgcgcagtgctaactatgaagcagaccctttcgtacaggagtttcagtttaaggtgcgagacgagatggcccacgtgacggggcgcgtgttgcctgcgcccatgctgcagtacgggggcagggtgagcacagagcactttatgaatcgcactgtggccacacccagtcatggagtttgggacatgagaggaaaacagttccacaccggggtggagatcaagatgtgggccatcgcctgcttcgccacacagagacagtgcagagaagagattcttaagggtttcacagaccagctgcgtaagatctcaaaggatgctgggatgcccatccagggtcagccgtgtttctgtaagtacgctcagggagccgacagcgtggagcccatgttcagacacctgaagaacacctactctggactgcagctcatcatcgtcatcctgccgggaaaaacacccgtctacgcggaagtgaagcgtgttggagacactcttctaggaatggccactcagtgtgtgcaggtgaagaacgtggtgaaaacctcgcctcagacgctctccaacctctgcctcaaaatcaacgtcaaactcggcggcatcaacaacatcctggtcccgcatcaacggccttcagtgttccagcagccagtcatctttctgggggcggacgtcacacatccacctgctggagatgggaagaaaccatccattgcagcagttgtgggcagtatggacgctcatcccagcaggtattgtgccacagtgcgggtccagaggcccagacaggaagtgattcaggacttggcctctatggtgcgagagctgctcattcagttctacaagtccactcgctacaaacccaccagaatcatcttctacagggacggtgtatctgagggacagtttagacaggtgttgtactacgagctgctggccattcgagaggcctgtatcagtctggagaaggagtaccagcccggcatcacatacatagtggtgcagaaacggcaccacacgcgcctcttctgtgccgatcgcaatgagagggtgggtcgcagcggaaacattcctgctggtacgacagtagacacagatatcacgcacccctacgagtttgacttttacctctgcagtcatgctggaatacagggcactagccggccctcccactaccatgtcctgtgggacgacaactgcttcactgctgacgagttccagctgctcacctaccagttgtgccacacatacgtgcgctgtacccgctccgtctccatccctgcgccagcctactacgcccacctggtggccttccgtgcccgctaccacttggtggacaaagaacacgacagtgccgagggcagccacgtctctgggcagagcaacggccgggatccccaagcgctggctaaagctgtgcagattcaccacgacaccctgaggaccatgtactttgcctaagccagctcacatcaagcccacgttcgactagtcatgcacacgaaaccaacaaccacatacacatgcagtcacacctcccaaaagcctgtgcgtcccgaagggtcgatctctgtggcgatggaagcacggctcagcccgacactgtgcagcaggatgaggaaggcctgctttaattccacacgtggacctcggcgctattatgcaatattaaaccagccaactgaggccgttctgtcatatcccctccaaacctttgacaacaaccaaaagggaagcccgtctatatccctgctcctcttcctgtctgtagacgtaatgtttaattacttcccctgcccgtcacgccttgtacctcaacgttttctaacagcacttttcccccctattatcctcccccttcccccaaaatgagattcgaaaggaagcagatggaaagactgagttgggaagaaaggaagtttcggggatagaggcatcacacgctcttactagcaattttctcaatgtacttttctcccgttctcaattatttttgtagccaatggtgacttaactacgtgttaaagtgtcagtcgtgaagaaccaattgtgcgttagagttttaaaacagaaaaacaagcttttattgttagaaaagtgccattgccgagcgtaaagtcctttatgacgttccccgccagcggggcagaaagctgaccgttgatttttaaagctaatgttcccattttacgctcagtaaaatctacctggcctgtgagctttcatgtactacaatgcaaagtgatcatgttttgatatgctttttcttttttctttttttaacacccaaattccctccaatatttatgtcttgatcaacgcagctcctagcagatttgtcttgttcacttcccttctgcactttgtctcaatttaaaatgcatagtccgtgttgtgacagatcatagtttagtgcaataatccattcgcgggatgagtacctcctgtgcaaagactcacagttcgctggaagtccgtagtggtgaaaaaggcactcttgctgttggtttttaaggtggaggtgtggcacttttatagttttctgaaagtagaggtgatgattgaccatagtgtgttaacaagtgcctgcttgatgttggggaggtcgaacagaattggatagatgctgtgggaaataacgaacgcaactcatctagtccaaacaagggcacagttgcgaacacattcgactgtacggccttaacaaaatctcttaacgttaacgtcttggttttcactgccctgcctgttcaaaggacttcgctgctattgtacttgttaaagtcagactggcgggttcatgcttcggtttcgcccaagcacgagggaccgattggcagctctgggccgtgtgtttaaggagaagctctcacagcacctgatgtgtgctctttcactggagggccactaggggtcagtatagtattttacttgaagagtgcatctcggtgcagtgagcgttgggtgccttactgtctctgtgcgccatctgaagcatgtccagctgtttaaaggtgataatctgcactaaacaatgcgtaatgcatggtgctattaggtgcggcccatggcggcatcagcgatttggcggagacttaatgtgtaacgatgggaacatggtatgcataaatggtgctggttgcatatatttctctcactccgagggggagattctcatcaggacagacatttggtcgttttgtgtgtttgttgtttgttttgttgatttgcatttgcatttgattttattgcgtagaaaggtacagtactgtggccagtcagaatacgattacggtcaattgcacaaagaaagtggaagattttaatgcatagcctaattatgacttttcaatttcagcccttttcaaaatccagatctctttatgcaatcagatgtttgctttttaaattctaaagtatgaataatcgcctgttaagaatgaaactaacgcagtgggatgttatatagagggttttctgcagggtttttaaaaaaagtgtgcattagaggtcggacctgtagaggaaggtacatgttttctgcccatcaggcctatcaaaagtggtttaagtgaggctgtaagcatcaaaacagcagctactgaaattgagcccttttttatttcaccttccctaataaaaccactattggtgcttcagagccttgaaagtaaaaccgcctgtgtgcggatgccaaacagctatcagtttaagaagcaatcttgatgaggtgtcatgctattttatttttgtgtagttttaacttcatatatatatatattactaacccttgcctcttgtttaggcaagaaatggatagttcggttataagtgattgtcattatttactcgcccacatgacttttctatcgctgaacgtggtgtacgtggtgttttaaagagcgtttgagttgctgtccatgcaacaaaaggcaaggatatcagtaaatgattgtcattttggagcttgctacactgtaaaaaaaaagtgtgctgccttaaattttaaagttgagtcaacttaaaaagtgaagttgcactaagtgacaacttggatatttgagttgacaacttaatattttaagtaagcacaaacttttcagtctatttttgttcagtccaagtcaacagctttagacaacgttctgtgaaattgcttcttatgaaagaagtgaatgtttgaatgacatgagggtgagaaaatgcttatgtgattgtctaatttttatctgaacgattcttcaaagcgacgaacggttttacccgaagatcgtacagagtgttagtgatgtatcggctgtggtagcagatgttgttgcaggggactcggttggttcgtagcagagtttcagtgcttgagtttgttgcctgacgtggcttccgttatccgtaaacctagaagtcaaacagccacatcccagtcagacaacgtattttctcctctcctctctcgtcttttaatttccagctttttgggagggtcttcttttagggtctaaaacgttacacgagttcagagttcatagaaagtgcctttcctcatgatggtgctgaataatctgagactaaagtgagaaatcaaagagcatattagtcttattcttaccttgaagaaggtgttgcacacatgcaaacacgcacgaacacattttatctcgatcgggaggctcggtgcctctttgtatattcagatatattatagtgacgatgatatatgccaagtatacgagcgaacggttctgtatcatactgtattaggagtccggaccaatgcaatggaagcgttcttataggtgaatgtgctatctttagatgggggcagagcggacactcatatgacacggcctgaccctggacaggaaagcctagcacagcactttaaagactgccacctaaacaaggaaacaaagtcacagtgctttgctctccttttccttttccccccataaacccttttttcatgtactccagcaaaagtctctagctcttgaagtctttgctgagggaaacttgagcgagattaattgaggtttacacaaggctccggtgacgtctttttggacttttttttattgccgtgactgatcgatccctctcaatgtgcaatggcccgtatagatctacatacagtatatgttttaaacatgagattgacccccatggcctgtgttttgtcacatgggtgcccgttggtccaggaggatatacctcgatgtagatctcaaaacactgcacctcacttaccttggtatgctgcaaatgttatatagaatgggtggatctcacgaaaatacacatcctgctcacgtttcaaattatgttttgcatgacaaaaaggaaggctattttcgtgcattgcggcaattaaacaaatctccccctccccaaattctgatgcatttgtacaccaccgtaatattgcttttcatttacgcagatttaaatgtaataatgcacatcgttctgttactattgaggtcgatgggaattacaaaactactcaactgggagaaattatgaaaataatgcaacaattgtatttgttgctataatatgcttaatttttgtccgttatgcttaattatattttccattaggaaaattaagccttttttggcattgtgaatatttttgagactattaaatgcatctccaaattctcattatgcaaacaaaaatattaaactgtattcatacatcatggtagtatttgtgcattgccttccatttatgctgatttaatgtaatacagcaaatatcattttatcaccattgatgggaactacaaaaccgctcaaaacgttgagagaaaagtgcttaattctcgtgaaaataatacaacagatgtattcgttgttataatattgccttattttcatgcaaaatgtcatttctagtgcacgacacacaaataagcaattgtattttgcataaagaaaatgaaattactatttctatgatgattgaatgcattcccccctctgcaaattctcatttatgcaaaccaaaatatatatatataatattaaaatgtattcatatatcatgtagtatttgtcgcattggctttcatgtatgctgatttaatgtaaggcagcaaaaatgaattacaaaacgccgtttgggagaaattcgcttaatcgttgtggaaataatgcaagaaatatcggcatcgttttccctgtgcaaaatatacttttcctttgattttggggtggatatgttttcttgagattcatccagaaattacgttgattacagtatattgcttgactaatgcctgacaacaatatccttaacgattttagagcaagaagtctaattgttagaaaacaagttgtttttaaatcataacacgtatctttttcttcttttattgagccaacttttttttattttgttgtcctatttaaatgtgttttgtttttttcttttaatatattggaaatatatatgggaaatatttaacggtgtatttattgagggagtgctttcgcggctggagtctccgcatcgttggccagagtcacgggggggcgagttgggggtcggggggtcagacgccctcgacgtcatcctctcacaagcacaaccacagagtgggcggggccggcacaagcctgaagtgttggatgacatcacttcctccttgcccttttgactagatcgtccacctcattcttcagctctccagcgaagaggctcgtcgcatctcatctctttacgggacggagaaccacactgactttattccaaaaagataaaaagctgattgttttcatccagagctgctaaactcttagccatacacgtttccctggaaggatgtttgatgcgtccggtgctttaagtggagtcgagaagcagatactgctatttgtccttcatcatttgcttgagttcaattgtcttcgtgccagagagggggtcccattccactcagaaatatataaatacattttta

//