LOCUS       XM_067255313            2970 bp    mRNA    linear   VRT 13-AUG-2024
DEFINITION  PREDICTED: Osmerus mordax argonaute RISC component 4 (ago4),
            transcript variant X7, mRNA.
ACCESSION   XM_067255313
VERSION     XM_067255313.1
DBLINK      BioProject: PRJNA1145451
KEYWORDS    RefSeq.
SOURCE      Osmerus mordax (rainbow smelt)
  ORGANISM  Osmerus mordax
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Stomiati; Osmeriformes;
            Osmeridae; Osmerus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_090067) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_038355195.1-RS_2024_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/12/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Osmerus mordax"
                     /mol_type="mRNA"
                     /isolate="fOsmMor3"
                     /db_xref="taxon:8014"
                     /chromosome="18"
                     /sex="male"
                     /tissue_type="muscle, testes, liver, kidney, brain, misc"
                     /dev_stage="adult"
                     /geo_loc_name="Canada: Placentia Bay Newfoundland"
                     /lat_lon="47.431645 N 53.826133 W"
                     /collection_date="2019-10-17"
                     /collected_by="Amber Messmer"
     gene            1..2970
                     /gene="ago4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 3
                     Proteins"
                     /db_xref="GeneID:136961841"
     CDS             232..2490
                     /gene="ago4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X7"
                     /protein_id="XP_067111414.1"
                     /db_xref="GeneID:136961841"
                     /translation="

MLLDALSGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGGWGPAGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRVSTDTGRDCGRGLSPQNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDLSQEQLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHYDTQHTMYFA"

     misc_feature    334..486
                     /gene="ago4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    487..867
                     /gene="ago4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(661..663,706..708,748..750,760..762,814..816,
                     835..837,841..843)
                     /gene="ago4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1006..2361
                     /gene="ago4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1465..1467,1477..1479,1513..1524,1531..1533,
                     1555..1557,1564..1566,1576..1578,1588..1590)
                     /gene="ago4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1669..1671,1675..1677,1915..1917,2329..2331)
                     /gene="ago4"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

aatgtttaaacagaagctgactgtgcccctacattttttcacttgggggcacatgtgctccagcaaaaaaaagttggcgtagagccctgaaggaggaaccaatttcacacttgaaatcctgtcctagtttgggaaaactagtttgtggacctggaagtgaccctccctggggaggggaaggaccagacgttcaaagtgtctctgcagtgggtgtcagtggtgagcctgcagatgctcttggatgccctgtctggacacctgaacgaggtccctgaggactccgtgcaggccctggacgtcatcacacgccacctgccttccatgaggtacaccccagtgggccgctccttcttttcccccccagagggctactaccaccccctgggcggaggccgggaggtgtggttcggcttccaccagtccgtccgtcctgccatgtggaacatgatgctcaacatcgatgtttcagccacagcgttttaccgagcccagccagtcatagagttcatgtgcgaggtcctggatatccaaaacatcaacgagcagacaaagccactcaccgactcccaacgcgtcaagttcaccaaggagatccgaggtgggtggggacctgcaggtctgaaggtagaggtcacacactgtggccagatgaagaggaaataccgtgtgtgcaatgtcacacgtcgccctgccagccaccaaacgttccctctgcagctagagaacggccaagccatggagtgcactgtagcgcagtacttcaagcagaagtacagtctgcagctcaaatacccccacctaccctgtctgcaagtagggcaagaacagaagcacacttatctgcccctggaggtttgtaatattgtggcagggcagcgctgcatcaagaaactaacagacaaccagacgtccaccatgatcaaagccaccgctcgctccgctcccgacagacaggaagaaatcagccgactggtcaaaagcaacagcatggtgggtggtccggacccctacctgaaggagtttggcatcgtggtccacaacgacatgacggaggtgacaggccgtgtgctcccagcacccatgctgcagtatgggggccgggtcagcaccgacaccggaagggactgtggcaggggactctctccgcagaataagacggtggccacgcccaaccagggcgtgtgggacatgagggggaagcagttctacgccggcatcgagatcaaggtgtgggctgtggcctgcttcgccccgcagaaacagtgcagagaagacctgctcaagagtttcacagatcagctgcgtaagatctctaaggacgctggcatgcccatccagggccagccctgcttctgcaagtatgcccagggggcagacagcgtggagcccatgttcaaacacctcaagatgtcctatgttggcctacagctcattgtggtcatcctgcccggcaaaacgcctgtctacgcggaggtgaagcgtgtaggagacactctgctgggcatggccacccagtgtgtccaggtgaagaatgtggtgaagacctccccccagaccctctccaacctctgcctcaagatcaacgccaaactggggggcatcaacaacgtcctcgttccccatcagaggccctccgtgttccagcagcccgtcatcttcctgggcgctgacgttacccaccccccggcgggtgacggtaagaagccgtccatcgcagcggtggtgggcagcatggatggccatcccagccgctactgtgccaccgtgcgggtgcaaacctcccgccaggacctgtcccaggagcagctttacagccaggaagtgatccaggacctgaccaacatggtgagagagctgctcattcagttctacaagtccacccgcttcaagcccacacggatcatttactaccgaggaggagtgtcggagggccagatgaaacaggtggcttggccagagctgatagccatcaggaaggcgtgtatcagtctggaggaggactacagaccgggcatcacctacatcgtggtgcagaaacgccaccacacccgcctcttctgctccgacaaggctgagagagtgggcaagagtggcaacgttccggccggcaccacagtggacagcaccatcacacatccctctgagtttgacttctacctgtgcagccatgctgggatccagggcaccagtcggccctcccactaccacgtcctgtgggacgacaactgcttcacggcagacgagctccagctgctgacctaccagctgtgccacacctacgtccgctgcacacgctccgtctccatccctgcgccagcctactacgcccgcctggtggccttccgcgcccgctaccacctggtggacaaagaccacgacagtgcggagggcagccatgtgtcagggcagagtaacggccgggatccccaggccctggccaaggctgtccagattcactatgacacccagcacaccatgtacttcgcttgaaccacaccagcagggacagcgcaacttcctctctatcctctctctctctgtctctgtttttttgccagtctctctctctctctcttccttgcgtcttgatctgcacctgagcgacgagaagccagtggaattcggcctcgttgtcctccccacttcccactcgggggtgggaaacaaacaacgtttggcgttcccacaatcaagtcgccttctaccagcaaaagcaggcagtacaggacccgcctgggtcgccactgcgcagagtaaaatccccacacttacacagttgagccatttctattgagttatcgatcatttatttcggttttgaatgtctatttttgtttgtttttgtaatatacactgagcgcggggcggactggcgctaaccgccagggcagctctttagctctcccagctaagcaggactctactttacctcaaagcccttttggggcgaaatctgtcacaaggtcttgg

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