LOCUS       XM_045782422            4383 bp    mRNA    linear   MAM 05-MAR-2022
DEFINITION  PREDICTED: Ursus americanus argonaute RISC catalytic component 3
            (AGO3), transcript variant X1, mRNA.
ACCESSION   XM_045782422
VERSION     XM_045782422.1
DBLINK      BioProject: PRJNA795466
KEYWORDS    RefSeq.
SOURCE      Ursus americanus (American black bear)
  ORGANISM  Ursus americanus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Carnivora; Caniformia; Ursidae;
            Ursus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_025578495) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Ursus americanus Annotation Release
                                           100
            Annotation Version          :: 100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 9.0
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Ursus americanus"
                     /mol_type="mRNA"
                     /isolate="UA15103"
                     /isolation_source="kidney"
                     /db_xref="taxon:9643"
                     /chromosome="Unknown"
                     /ecotype="Eastern"
                     /country="USA: Penobscot nation, Maine"
                     /lat_lon="45.745671 N 68.499951 W"
                     /collection_date="2015-05-18"
     gene            1..4383
                     /gene="AGO3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 1 EST, 30 Proteins, and 100%
                     coverage of the annotated genomic feature by RNAseq
                     alignments, including 15 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:123782310"
     CDS             115..2697
                     /gene="AGO3"
                     /codon_start=1
                     /product="protein argonaute-3 isoform X1"
                     /protein_id="XP_045638378.1"
                     /db_xref="GeneID:123782310"
                     /translation="

MEIGSAGPVGAQPLLMVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSIKFVSRVSWHLLHEVLTGRTLPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA"

     misc_feature    331..615
                     /gene="AGO3"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    643..795
                     /gene="AGO3"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    796..1158
                     /gene="AGO3"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(952..954,997..999,1039..1041,1051..1053,1105..1107,
                     1126..1128,1132..1134)
                     /gene="AGO3"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1291..2568
                     /gene="AGO3"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1702..1704,1714..1716,1750..1761,1768..1770,
                     1792..1794,1801..1803,1813..1815,1825..1827)
                     /gene="AGO3"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1906..1908,1912..1914,2122..2124,2536..2538)
                     /gene="AGO3"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      4383
                     /gene="AGO3"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

ctcgtcttggggccgagtgagggtgcctgtcgcgccgcgtcgccccctgcgctgcctccttgcccccagtggtgggcgcccttctcgttcgaagcactccccccagctccatgaatggaaatcggctccgcaggacccgttggggcccagcccctactcatggtgcccagaagacctggctatggcaccatgggcaaacccattaaattgctggctaactgttttcaagttgaaatcccaaagattgatgtctacctctatgaggtagatattaaaccagacaagtgtcctaggagagtgaacagggaggtggttgactcaatggttcagcattttaaggtgactatatttggggaccgtagaccagtttatgatggaaaaagaagcctttacacagccaatccactacctgtggcaactactggggtagatttagatgttactttacctggggaaggtggaaaagatcgacctttcaaggtgtcaattaaatttgtctcccgggtgagttggcacctgctacacgaagtactgacaggacggaccttgcctgagccactggaattggacaagccaatcagcactaatcccgtccacgccgttgatgtggtgctacgacatctgccctccatgaaatatacgcctgtggggcgttcctttttctcagctccagaaggatatgaccaccccctgggagggggcagggaagtatggtttggattccatcaatctgttcgacctgccatgtggaaaatgatgcttaatattgatgtttctgccactgccttctacaaagcacaacctgtaattcagttcatgtgtgaagttcttgacattcataatattgatgagcaaccaagacctctgactgattctcatcgggtaaaattcaccaaagagataaaaggtctgaaggttgaagtgactcattgtggaacaatgagacggaaataccgtgtttgtaatgtaacaagaaggcctgccagtcatcaaacctttcctttacagttagaaaacggccaaactgtggagagaacagtagcacagtatttcagagaaaagtatactcttcagctgaagtacccacaccttccttgtctgcaagtggggcaggaacagaaacatacctacctgccactagaagtatgtaatatcgtggcagggcaacggtgtatcaagaagctaacagacaatcagacttccactatgatcaaagcaacagcaagatctgcaccagatagacaagaggaaattagcagattggtaagaagtgcaaattatgaaacagatccgtttgttcaggagtttcaatttaaggttcgggatgaaatggctcacgtaaccggacgtgtacttccagcacctatgctacagtatggaggacggaatcggacagtagcaacaccaagccatggagtatgggacatgcgggggaagcaatttcacacaggagttgagataaaaatgtgggctatagcttgttttgccacacagaggcagtgcagagaagaaatactgaagggtttcacggaccagctgcgtaagatttctaaagatgcagggatgcccatccagggccagccttgcttctgcaaatatgcgcagggggcagacagcgtggagcccatgttccggcatctcaagaacacctactctgggctacagctcattatcgtcatcctgccaggaaagacgccagtgtatgcggaagtgaaacgtgtaggagatacacttttgggtatggccacgcagtgtgtccaagtcaagaatgtaataaaaacatctcctcaaactctctcaaacctgtgcctaaagataaatgttaaactcggagggatcaataatattcttgtacctcatcaaagaccttctgtgttccagcaaccagtgatctttttgggagcagatgtcactcatccaccagctggtgatgggaagaagccttcgattgctgctgttgtaggtagtatggacgcccacccaagcagatactgtgccacagtaagagttcagagaccccgacaggagatcatccaggacttggcctccatggttcgagaacttctcattcagttttataagtcaactcggttcaaacctactcgtatcatcttttatcgggatggtgtttcagagggccagtttaggcaggtattatattatgaactactagcaattcgagaagcctgcatcagtttggagaaagactatcaacctggaataacctacattgtagttcagaagagacatcacactcgattattttgtgctgataggacagaaagggttggaagaagtggcaatatcccagctggaacaacagttgatacagatattacacacccatatgagtttgatttttacctctgtagccatgctggaatacagggtaccagccgtccttcacactatcatgttttatgggatgataactgctttactgcagatgaacttcagctgctaacttaccagctctgccacacttatgtgcgctgtacacgatctgtttctatacctgcaccagcgtattatgctcacctggtggcattcagagccagatatcatctcgtggacaaagaacatgacagtgctgaaggaagtcacgtttcaggacagagcaacgggcgagatccacaagctcttgccaaggctgtacagattcaccaagataccttacgcacaatgtactttgcttaaaaagtccaagaatattctctgagaggaagaactgaaagatgcatcgacatacagcatatgtttcccgtggagtcagttgagtggggatgcctccagccatacagcaaccaacactgtgtggggaccagggtctgatttttatgttgatacaaggaagattgtttacttcatcaaggaacacagcaccattatgcaatatgaaaccagccaactgcttttttgtgcggtctccttaggaagtatcgccattgttttgttttcactttcttgtagtctaacccttttaatgcctttacctcaagttgcttggcagcacaactatctttgcaaaaaaaaaaaaaaagtaaagaaaaagtacatgacagtttaaaaaaaaacacacctacatgaataatcaaagtgattgttcacgattatgtgtgcaaaaaattaatgtgcattcgtgtattcttgtaaaaggtatctgtgtatattttaaatatatacatgtatacttcatatgcatatatatctggatctacactgataatagatatatatgtgtctgggcgtgtattttacagttcattccactggggaactttctttcccttttattattttcccaccaccactttgatttttctctctacctcccttgcctcctcacctacgttttttccctaatgctaccagtgacactcagatgttcatgtatctggttcatttgggtgtgaagcatggcaaactagatttttatttttattttgcacaccctactcctgtctcttgtctcatacacaattgcaagtcctcaactcttgatattgctttcttaacaaacaaaattatatatatatatatatatacatatatgtgtgtgtgtatatatatatatatatataatgtttctgtgagagtgcgtgtctgccccctcctgctctgaaattacatttgccatttgtgcaatgtgtacataagcagtagcaactgtaaactattctttttcattttgttaaagctgctataaatataaaaccttgtcagaagttgtaactcagaaatttctcctgtaagtgatatgtattctaaagccttctgtttctatgggttatacatgaaaatcccttatagcttttataaagggcaaatattctaaatgattggaactctttagggttgaggcctctgaaattgggtggagagagagaaggggttcttgagggtttttttaattttaattttttttttttgctagtttacttgctgcctctcataccttaatgtgattttcatatatatatatttttatatatatgtgcgtatatataaacatatatgtatgtgttttgaagtacagcttccttttctttcatatttattagaatagtgctttaataattagagaactgtattggcaacatcttttatagaaatgtaatcatgtttattttaagaactgacaacttgttcttgctgtcttttagtgcaataagcagttctaaaggaaagctgtgtctgtttgatatctttaccacggatagtgggagaaacaggcacctccataattttaaaagggcaaagtaatacagccttaatttcagaaggaaagttttgtaatttttcaagtttaaagcatatcttctaaaaattgtaatgaaaatgaaggaagttaaatttcctaagtgaatctgaccttgaatatta

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