LOCUS       XM_023649005           14656 bp    mRNA    linear   MAM 07-AUG-2024
DEFINITION  PREDICTED: Equus caballus argonaute RISC catalytic component 2
            (AGO2), mRNA.
ACCESSION   XM_023649005
VERSION     XM_023649005.1
DBLINK      BioProject: PRJNA19129
KEYWORDS    RefSeq.
SOURCE      Equus caballus (horse)
  ORGANISM  Equus caballus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_009152.3) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_002863925.1-RS_2024_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/01/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14656
                     /organism="Equus caballus"
                     /mol_type="mRNA"
                     /isolate="Twilight"
                     /db_xref="taxon:9796"
                     /chromosome="9"
                     /sex="female"
                     /breed="thoroughbred"
     gene            1..14656
                     /gene="AGO2"
                     /note="argonaute RISC catalytic component 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 1 EST, 16 Proteins, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 5 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:100069541"
                     /db_xref="VGNC:VGNC:15164"
     CDS             257..2698
                     /gene="AGO2"
                     /codon_start=1
                     /product="protein argonaute-2"
                     /protein_id="XP_023504773.1"
                     /db_xref="GeneID:100069541"
                     /db_xref="VGNC:VGNC:15164"
                     /translation="

MDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVAIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA"

     misc_feature    392..616
                     /gene="AGO2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    644..796
                     /gene="AGO2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    797..1159
                     /gene="AGO2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(953..955,998..1000,1040..1042,1052..1054,1106..1108,
                     1127..1129,1133..1135)
                     /gene="AGO2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1292..2569
                     /gene="AGO2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1703..1705,1715..1717,1751..1762,1769..1771,
                     1793..1795,1802..1804,1814..1816,1826..1828)
                     /gene="AGO2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1907..1909,1913..1915,2123..2125,2537..2539)
                     /gene="AGO2"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

agaggggaccgcgagcccggattactgccagccggggtgcgggtaccgcgagcccgggttaccgtgagcccgggttaccgcgagcctgggtggggggactgcaagccggagtggggagaccgcgagcccgggttacctgctcgcgcctcctgcaccgccaccaccgcccatccaaggatatgccttcaagcctccgcctagacccgacttcgggacctccgggagaacaatcaaattacaggccaacttctttgaaatggacattccaaaaattgacatctatcattatgaattggatatcaaaccagagaaatgcccgaggagagttaacagggaaatagtggaacatatggtccagcactttaaaacacagatctttggggatcggaaaccagtgtttgatggaagaaagaacctctacacggcgatgcccctcccaatcgggagggataaggtggagctggaggttacgttgccaggagaagggaaagatcgcatcttcaaggtggccatcaagtgggtgtcgtgtgtgagcttgcaggcgttacatgatgcactttcggggcggctgcccagtgtcccctttgagacgatccaggccttggacgtggtcatgaggcatttgccatccatgaggtacacccccgtgggccgctccttctttaccgcctccgagggctgctccaacccgcttggtgggggccgagaagtgtggtttggcttccatcagtccgtccgaccttctctttggaaaatgatgctaaatattgacgtctcagcaacagcgttttataaggcacagccagtgatcgagtttgtttgtgaagttttggattttaaaagtattgaagagcaacaaaaacctctgacagattcccaaagggtgaagtttaccaaagaaatcaaaggtctaaaggtggagataacgcactgcgggcagatgaagaggaagtatcgcgtctgcaacgtgacccggcggcccgccagccaccaaacgttcccgctgcagcaggagagcgggcagacggtggagtgcacagtggcccagtacttcaaggacaggcacaagctggttctgcgctatccccacctcccatgtttacaagtcggacaggagcagaaacacacctaccttcccctcgaggtctgtaacatagtggcaggacagagatgtataaaaaagttgactgacaatcagacctcaaccatgatcagagcgactgccaggtcagcgcccgatcggcaagaagagattagcaaactgatgagaagtgcaagttttaataccgatccgtatgttcgtgaatttggcatcatggtcaaggacgagatgacagatgtgactgggcgggtcctccagccaccctccatcctctacgggggcaggaataaagcgattgccacccctgtccagggcgtctgggacatgaggaacaagcagtttcatacgggcattgagatcaaggtgtgggccattgcgtgcttcgccccccagcgccagtgcacggaagtccaccttaagtccttcacagagcagctcagaaagatctccagagatgctgggatgcccatccagggccagccgtgcttctgtaaatatgcccagggggcggacagcgtggagcccatgttcaggcatctgaagaacacgtacgccggccttcagctggtggtggtcatcctgcctggcaaaacccctgtttacgccgaggtcaagcgcgtgggagacacggtgttagggatggccacacagtgtgtgcagatgaagaatgtgcagaggaccacgccacagaccctgtccaacctctgcttgaagatcaatgtcaaactgggaggcgtgaacaacatcctactgccccaggggaggcctccagtgttccagcagccggtcatctttctgggcgctgatgttactcacccacccgctggggacgggaagaagccttctatcgccgccgtcgtgggcagcatggacgcccaccccaatcgctactgtgccaccgtaagggtccagcagcaccggcaagagatcatccaagacctggccgccatggtgcgcgagctcctcatccagttctacaagtccacgcgcttcaagcccacacgcatcatcttctaccgcgacggtgtctcggaagggcagtttcaacaggttctccaccacgagttactggccatccgcgaggcgtgtattaagctcgaaaaagactaccagccggggatcactttcatcgtggtacagaagagacaccacacacggctcttctgcactgacaagaacgagcgggttgggaaaagtggaaacattccagcaggcacaaccgtggacacgaagatcacccacccgaccgagtttgacttctacctgtgcagtcacgccggcatccagggaacaagcaggccttcccactatcacgtgctttgggatgacaatcgtttctcttccgatgagctgcagattctcacctaccagctgtgtcacacctacgtgcgctgcacgcgctccgtatccatccctgcgccagcatactatgctcacctggtggccttccgggccaggtaccacctggtggataaggaacatgatagtgctgaaggaagccatacctctgggcagagtaacggccgagaccatcaagcgctggccaaggcggtccaggttcaccaggacacactgcgcaccatgtactttgcttgacatgtttagtgtttacgattgtgtaccgagttggattcacatgagaccagctacactcagaccaacagatgcccagcccttccgtgacagccagcatcgaacatgagacgtcattgatttcagtagattctccattttccagaatgccttccgtcccagatttcacacttggattttgaactgcagacctgtatgagaccccgttgtcctaggaaatatggtttgccaaaatctataagctgcttactaaaatagagtcccgtgtttcctaaaaatctcctaaaaccagtctatgaactcagggctttaaaacgtttttaatttatttggtcattcaatttacttgtttttaacacatgattctatatgaaattgatgggctcaaactagctgtgaacgttctctgagagtgagagcagcacaaaacacgattgtggttttaaagccttgaacattctgatgttgtcactaaagtttatttcaaagtgatgctagtgtgctcctgacttggctttcacaagcccttcgggacccgagggcaggtggcatgccgaggcgcccacaggtgggtctgttctgccactgcttgcctttctcacagcttgaaacaaggagcagtggcagaagatgggccacgtgtgtttacaacatttataggtccagagagattggcagacaagtgccattttaataaaaatttatttaaaaaaagaaaaaagacaatatgctgacagtctaatcataagatttgtcctataggactgtgacgtttattttccaaagtttctaaagaatacgggtctgtggtgataaatacagtacaatcctttttcactgttatgtctttaatgtaagagaaaaatatatatatctggtttgcagtattaatgtgatagatagatgggatagatgccatttttcattttattgactacggggtgcttcttgtgatctgtttagcgttatcaagagtgtttcggagcctggagcctggagcctggaccacctcctgtggttctcagtgcttaagtacaggaatatgcagttgacttaatgtgacagcagcctgcccccctcagcgtagattgtgtagcctgtaccttcaggaggaccgagcacactcacgaggccatcactccctccctctctgtcccctcgcccccgtgttcttgctcagccgattaagcacaaatggaatgtatgtattctcccattcctgggagacacgtccatgtatcagagtgtacctctctctgtgtgtggttttgttagactcgtttcataggatctatgcactgaaacacacagaaagctgttggcagtggctccctgttgggggcgaggccagcagtcagcaacagagccctggcctccggatggctgactgccaggccagcagcagtggcagacccttctgctaagatttgcattctgggccccagatgtgggtttgccaatctggaaaccattgcactaagagtgataccatggacagcttgaaaagggtaagaacagacacgtctgtttgtgctaactggctttttgttcttgtaggcagttgtattcagagtcaagtccacattcaatgtagagattctaggaaggagacgacccttccctagagctgctttgtgctctgtaggtttccactccccagcccggcctgacatttacctctggcactgcggtggagtgagggtcccgatgggggtgcctcgtgctggaataagcgccacctctgtctcctggtctgacggctccaaggggtgggaagggcagggaaccgcaggccgagctgtaatggacactcagcctggattttggcagcgataacaaagggatgacaatattatttgtgaagctatctactccgagaactgtgtgcgtgtgtgtgtgtctgtgtgtgcatgtgggtgtcttattaatggcattagtctcttcttgctgtatctttttttctttgtctttttaaatgacagaagcctctgctctaagaaacgttgcaattccctgtcgttttattggcaagtggcactttctcctatgttcagaagcataccaatacactcttggcttaaattaccagaagcattcaagctaaggagggaatcttttcactaatacgcttttaaaatctgaaattaggttagttttcagccatttttaggatcatctagaaccacgtgtctcaaaggtgtgtttttaaccacctgcaccaggatctcagtggtcggggcagtggcggtgcttggtaaaaatgcagaccccagggctgggagtggcatctgcattttacaccatt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