LOCUS       XM_019876096            4993 bp    mRNA    linear   VRT 01-JUL-2025
DEFINITION  PREDICTED: Hippocampus comes NRDE-2, necessary for RNA
            interference, domain containing (nrde2), mRNA.
ACCESSION   XM_019876096
VERSION     XM_019876096.1
DBLINK      BioProject: PRJNA359802
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Hippocampus comes (tiger tail seahorse)
  ORGANISM  Hippocampus comes
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Syngnathiaria; Syngnathiformes; Syngnathoidei;
            Syngnathidae; Hippocampus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017805485.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_001891065.2-RS_2025_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/30/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Hippocampus comes"
                     /mol_type="mRNA"
                     /isolate="QL1"
                     /db_xref="taxon:109280"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="muscle"
                     /dev_stage="mature"
     gene            1..4993
                     /gene="nrde2"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 4 Proteins, and 94% coverage of
                     the annotated genomic feature by RNAseq alignments"
                     /db_xref="GeneID:109519521"
     CDS             68..4741
                     /gene="nrde2"
                     /codon_start=1
                     /product="protein NRDE2 homolog"
                     /protein_id="XP_019731655.1"
                     /db_xref="GeneID:109519521"
                     /translation="

MALFPAFAGVTSDKVDDSPNELKWLNNKSFQTSDALSLHSRFLETKNEGLHKDLSLDRKDEDTVPSKKKKKHEKHGKKRKKHKEKVKRSTESSGSDSEPIFPSDLKRKQETDRPQAAAIPSSFSWLDDIPSPTAQPFCVDSKSDASNWTYKSLYRGDVARYKRKGISSLGLDPRRQEIRWEDTKKKHKGGDKKKGTDRYFFPACRLLLRSQPPLQTLPRVCESSDVTNSNPFLPLEDTVGERDGQPGGRAQISSVNPLGVYDSTTALWLQGKGKQDPTDHHDNHNSETGQEAQLLTGQTEEFNRKLREQPEDTQLWLKFIRYQDELSAAVFGGEEEQQGKELLERRKSSYKAVLEKKLSIAERAVATNPGCVALQRERLRICQELWEPSALAKEWKKLVFLHPNSGPLWREYLLFTQSYFSNFSVSKVNSAYGKCLSMLSAVRDGSMVSHPALPGIEEDMLDIFTKQCHFLRQSGHSEKALSLFQAMIDFTFFKPDSVRQLSTKQQVNAQIKHIVHQPMRNYILEDEEEEEEEEEVKDRSQPRWTVWLDVESSREAAHWLPWRPEKAKGQSEDDCEDPERQVLFDDIGPSLICLSSPELQLRFLLHFLSFLGLPVDTVLSAGLCHPGLLLEDLSLLTQGSDSRRALTSHNLPRPGMNSVGHMTTLQGTRKWVGLGKQGEKFVNAMFNMLQPVLPSEQRAVLSLSRMQYEKLKVFRCLHGNKKRLRSQSKISKRIAKSLLKEAENRSSLAMWREFALGPEVTSSDSRRALTSHNLPRPGMNSVGHMTTLQGTRKWVGLGKQGEKFVNAMFNMLQPVLPSEQRAVLSLSRMQYEKLKVFRCLHGNKKRLRSQSKISKRIAKSLLKEAENRSSLAMWREFAQLEWMLGNVEEARKVFSAATALGGAKGLCHPSLCELCLLWAQLEVEDGESGPGGVPTDRGASTAVCVLTTLTEGHALSPSSLSQPVSPVAILKARRSYEQALTAALSALNQDLCNPQADKKSHVDVREEKLRLKGLLGCYALFQYLTMGIKAASAVYSQARERMDELHRTITSDQNSRADADHSGSNLLVSRFAYECETVAVQQASLLKYHMSVSAFPLATLRDTLTSAISRWPSSGPLWSIYVQVENRYHSAGRARCFFHSVTRDNDSVVPRLFAIVAEQQRKQLVDAAQRSCDKSAALSVLPENGLSNRIRGLFESAVGSEMGVHCPLLWRMYIHYLVLFDDIGPSLICLSSPELQLRFLLHFLSFLGLPVDTVLSAGLCHPGLLLEDLSLLTQGRVDVREEKLGLKGLLGCYALFQYLTMGIKAASAVYSQARERMDELHRTITSDQNSRADADHSGSNLLVSRFAYECETVAVQQASLLKYHMSVSAFPLATLRDTLTSAISRWPSSGPLWSIYVQVENRYHSAGRARCFFHSVTRDNDSVVPRLFAIVAEQQRKQLVDAAQRSCDKSAALSVLPENGLSNRIRGLFESAVGSEMGVHCPLLWRMYIHYLVSEGKMDNAKGLFYKALQNIPWVKGLYMDAVQLFPEHIQEFVDLMTEKELRVRLPLEELDILLED"

     misc_feature    473..778
                     /gene="nrde2"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    order(473..499,506..511,521..526,530..532,536..580,
                     587..595,599..607,635..643,653..658,662..667,674..694,
                     716..727,749..778)
                     /gene="nrde2"
                     /note="MTR4 binding site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:412062"
     misc_feature    968..1903
                     /gene="nrde2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:462472"
     misc_feature    <3719..3811
                     /gene="nrde2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:462472"
ORIGIN      

gtttttgcgcgtcatagtacgtcattttctagcgactgtatgtttcccatcaacacagaccaaaaatatggcactgtttccagcctttgcaggagttaccagcgacaaggttgatgattcacccaatgaactgaaatggctaaacaataagagttttcagaccagtgatgccctgtctcttcatagtcgcttcttggagacgaaaaatgaggggctacacaaagacttgagtttagacaggaaagatgaagacactgtgccatcgaagaagaaaaagaagcatgaaaagcatgggaaaaaaagaaaaaaacacaaagaaaaagttaaaaggtcaacagaaagcagtggatcggactctgaaccaatcttcccaagtgatctcaagcggaaacaagaaacagaccgtccgcaggctgctgcaattccaagttctttctcatggttggatgacattccgtcgcccacagcacagccattctgtgtggattctaaatctgatgcgtccaactggacgtacaagtctctgtacagaggagatgtagcaaggtataagaggaaaggtatttcatcactgggtctggatcctcgcagacaagaaatacgctgggaggacacgaaaaagaaacataaagggggcgacaagaagaaaggaacagacagatatttttttccagcgtgtcgtctgctgctcaggtctcagccacctctccagaccttaccaagagtttgtgaaagcagtgatgtcaccaactccaacccttttctcccactggaagacactgtgggggagagagatggacagccaggtggcagagcgcagatctcatcagtgaatccgcttggcgtgtacgactccaccactgccctctggctgcaggggaagggaaagcaggacccgacagatcatcacgacaatcacaactcggagacaggacaagaagctcaacttttgaccggtcagactgaagagttcaacaggaaattgagagagcaacccgaggacacccagctctggctgaaattcattagatatcaagatgagttgagtgccgcagtattcggaggtgaggaagagcaacagggcaaagagttattggagcgtcgcaagtcttcttataaagccgtgctggagaaaaagctcagcattgccgagcgtgccgtggcaactaaccccggctgtgtggctctgcagcgggagaggctcaggatctgccaagagctttgggaaccatcagctctggctaaagagtggaagaaactggtctttctccacccaaatagtggccctctgtggagggagtatcttcttttcacccagagctacttcagcaacttcagtgtgtcaaaagtgaactctgcttatgggaagtgtctcagcatgcttagtgctgtccgggatggcagcatggtgtcccacccagccctgccagggattgaagaggatatgctagatatcttcaccaagcagtgtcatttcctccgtcagtccggacactcagagaaggcactctctctctttcaagcgatgattgactttacgttcttcaagcctgacagtgtacgacaactttccaccaaacaacaggtaaacgcacaaataaagcacattgtacatcaacctatgaggaattatatactggaagacgaggaggaggaggaggaagaggaggaggtgaaggaccgcagccagccacggtggaccgtgtggctggatgtagagtcatctcgcgaagctgcgcattggttgccttggagacctgaaaaagccaagggccaatcagaagatgactgtgaggacccagagagacaggtgctgttcgatgacatcggcccttctctcatttgcctgtcctcgccagagcttcagctccgcttcctcctccatttcctgtcatttctcgggcttccagtggacactgtgctatctgctggcttgtgtcatcctggactgctcttggaggacctttctctgctcactcagggtagcgactcccgacgagctctgacttcccacaacctgccacgccccgggatgaactctgtgggtcacatgaccacactccaaggaaccaggaaatgggtaggacttggaaagcagggcgagaagtttgtcaacgcgatgttcaatatgctccagcctgttcttccgtctgaacaacgagctgtcctgtcgctcagccggatgcagtatgagaaactcaaggtgtttcgttgcctgcacggcaacaaaaaacgtttacgctctcagagcaaaatcagcaagcggattgccaaatcactgctcaaagaagccgaaaaccgctcatccttggcaatgtggcgagaattcgccttaggtcccgaggtaacaagtagcgactcccgacgagctctgacttcccacaacctgccacgccccgggatgaactctgtgggtcacatgaccacactccaaggaaccaggaaatgggtaggacttggaaagcagggcgagaagtttgtcaacgcgatgttcaatatgctccaacctgttcttccatctgaacaacgagctgtcctgtcgctcagccggatgcagtatgagaaactcaaggtgtttcgttgcctgcacggcaacaaaaaacgtttacgctctcagagcaaaatcagcaagcggattgccaaatcactgctcaaagaagccgaaaaccgctcatccttggcaatgtggcgagaattcgcccaattagagtggatgctcggtaacgtcgaagaggctcggaaagtgttttctgcagccacggcattgggtggggccaaggggctgtgtcacccctccctttgtgagctttgtctgctgtgggctcaactcgaggtggaggatggagaaagtgggccaggaggggttcccaccgacagaggtgcatctaccgccgtttgtgtgctcaccacactgacagaggggcacgccttgtcgccttcttcgttatcacagcccgtctccccggttgccatcctgaaggccaggaggtcttacgaacaagctctgactgccgctttgtctgcattaaatcaagatctctgcaatcctcaagccgacaaaaaaagtcacgtggatgtccgagaagagaagctgagattgaaagggctgctaggctgctacgctttgttccagtacctcaccatggggatcaaggcggcaagcgcagtctacagccaggccagagagcggatggacgaactccaccgcacaataacgtctgaccagaactctcgcgctgatgccgaccactctggcagcaacctgttagttagcaggttcgcttacgagtgtgagacggtggcggtgcagcaggcgtcgttgctcaagtaccacatgagcgtcagtgcatttccactggcgacactgagagacacactcacctctgccatttcgagatggccaagcagtggccccctctggagcatctatgtacaggtggagaaccgttaccatagtgctggccgagcacgttgcttttttcactccgtaacaagggacaacgatagtgttgtgccacggctctttgctattgttgctgaacaacaaaggaagcaactggtggacgcagcacagagatcctgcgacaaaagcgcggccttgagtgtcctgccagagaacggtctcagcaaccgtatccgtggactgttcgaaagcgccgtcggatccgagatgggcgtccactgtcccttactttggaggatgtacattcactacctggtgctgttcgatgacatcggcccttctctcatttgcctgtcctcgccagagcttcagctccgcttcctcctccatttcctgtcatttctcgggcttccagtggacactgtgctatctgctggcttgtgtcatcctggcctgctcttggaggacctttctctgctcactcagggtcgcgtggatgtccgagaagagaagctgggattgaaagggctgctaggctgctacgctctgttccagtacctcaccatggggatcaaggcggcaagcgcagtctacagccaggccagagagcggatggacgaactccaccgcacaataacgtctgaccagaactctcgcgctgatgccgaccactctggcagcaacctgttagttagcaggttcgcttacgagtgtgagacggtggcggtgcagcaggcgtcattgctcaagtaccacatgagcgtcagtgcatttccactggcgacactgagagacacactcacctctgccatttcgagatggccaagcagtggccccctctggagcatctatgtacaggtggagaaccgttaccatagtgctggccgagcacgttgcttttttcactccgtaacaagggacaacgatagtgttgtgccacggctctttgctattgttgctgaacaacaaaggaagcaactggtggacgcagcacagagatcctgcgacaaaagcgcggccttgagtgtcctgccagagaacggtctcagcaaccgtatccgtggactgttcgaaagcgccgtcggatccgagatgggcgtccactgtcccttactttggaggatgtacattcactacctggtgtcggaaggtaagatggataacgccaaggggctcttctacaaagccttgcagaatattccctgggtgaagggcttgtacatggatgcagtgcagctatttcctgagcacatccaggagtttgttgacctgatgacagagaaagagctgcgagttcggttgccgttagaagaactcgacatactgctggaagactgaaacaaactcccccgccaaaactatcattggatagtccagcaggagaaagacgttgcattgatttcgccagatgaatgtggaatgctagtctctacctggacttgagaggagtaaactaaattaaaacaaaacaaaacattttagctgttttttttttagttgatattttcaataaatatcattttgtttataatttgtatatgaagttttgatgtttgaattgattaattaaagtgtgattttgtttctgaa

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