LOCUS       NM_001279306            3473 bp    mRNA    linear   PLN 08-DEC-2016
DEFINITION  Solanum lycopersicum argonaute 2A (AGO2A), mRNA.
ACCESSION   NM_001279306 XM_004233163
VERSION     NM_001279306.2
KEYWORDS    RefSeq.
SOURCE      Solanum lycopersicum (Lycopersicon esculentum)
  ORGANISM  Solanum lycopersicum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; asterids; lamiids; Solanales; Solanaceae;
            Solanoideae; Solaneae; Solanum; Solanum subgen. Lycopersicon.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JX493012.1.
            
            On Apr 12, 2015 this sequence version replaced NM_001279306.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: JX467706.1, JX493012.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA4079219, SAMEA4079221
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3473              JX493012.1         32-3504
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Solanum lycopersicum"
                     /mol_type="mRNA"
                     /db_xref="taxon:4081"
                     /chromosome="2"
                     /map="2"
     gene            1..3473
                     /gene="AGO2A"
                     /gene_synonym="AGO2A2"
                     /note="argonaute 2A"
                     /db_xref="GeneID:101249141"
     CDS             18..3146
                     /gene="AGO2A"
                     /gene_synonym="AGO2A2"
                     /codon_start=1
                     /product="argonaute 2A"
                     /protein_id="NP_001266235.2"
                     /db_xref="GeneID:101249141"
                     /translation="

MDRGNYRRGGGHDNAGRGGGNRGRGQGRGGGRGEPQIGGGRGGTHIGGGSQMGQPPQQWGNQPRGSGQYQIRGGAPQNQPGQNYPVNQNPGRGGTWVNQPVQRGGGGGGSAWPRPQQQGSGVPNTTWPRPPPQQQGSGSGVANAWARPPPQRSQQHGGGNQQQVVDRSPPQSSDPVQVDLGSLKITDQSPSSRQESSKEKRVPIARPDTGKIAVKSIALLANHFPVRFNPQSTIMHYDVDIQQRADGNRPVKKLTNKSVLHMIREKLCADDPTRFPLDKTAYDGKKNIFSAVQLPTGCFAVNWSDGEDARLRSYDITIKLVAELKLCKLKEYLSGSLSHIPRDILQGMELVMKENPTRCRTSVGRCFYSNEHLPDHDFRFGVAAYRGFQQSLKPTKGGLALCLDYSVLALRKPMPVLDFLKEYLGESNENTFRNNIRAAKGALVGLKVRVIHRRTSQKFLIKQLTDCKTREITFPLEDPEGINPPRDVLLVDYFRDKYQREIQFKDFPSLDIGKGNKKNYVPMEFCVLVEGQRYPKEDLDKDTALFLKNISLARPQDRRQAICEMVRAGDGPCGAVTRNFDIGVDRNMTRVPGRILPPPDLKLGGQNRLPVNDKCQWNLVGKSVVEGKALQRWALIDFSAQDRKPFFRLRVDEFVFRLKDRCRKLSINMEEPAVVHFTDMHVLSEVGKVHKLLDGVVNAAKREINGKLQMIVCVMTSKHNGYKYLKWVSETQIGVVTQCCLSTNANKGQDQYLANLCMKINAKLGGSNMELMDRLPNFGREDNVMFIGADVNHPAAKNVTCPSIAAVVATVNWPAANRYAARVCPQVHRTEKILEFGKMCADLVHTYKEINSVKPNKIVVFRDGVSEGQFDMVLNEELLDLAKAIYDSNYQPAITLVVAQKRHHTRLFPEGGPANVPPGTVVDTIIVHPSDFDFYLCSHFGGLGTSKPTHYHVLWDDNGFNSDSLQKLIYNMCFTFARCTKPVSLVPPVYYADLVAYRGRMFQEVLMEMNSPSSATSSSPTASFQQKFYDLHSDLQNVMFFV"

     misc_feature    <168..>470
                     /gene="AGO2A"
                     /gene_synonym="AGO2A2"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; Region: Med15; pfam09606"
                     /db_xref="CDD:312941"
     misc_feature    840..1073
                     /gene="AGO2A"
                     /gene_synonym="AGO2A2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    1101..1253
                     /gene="AGO2A"
                     /gene_synonym="AGO2A2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    1254..1604
                     /gene="AGO2A"
                     /gene_synonym="AGO2A2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1392..1394,1437..1439,1485..1487,1497..1499,
                     1551..1553,1572..1574,1578..1580)
                     /gene="AGO2A"
                     /gene_synonym="AGO2A2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1749..3023
                     /gene="AGO2A"
                     /gene_synonym="AGO2A2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2181..2183,2193..2195,2229..2240,2247..2249,
                     2271..2273,2280..2282,2292..2294,2304..2306)
                     /gene="AGO2A"
                     /gene_synonym="AGO2A2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2385..2387,2391..2393,2604..2606,2994..2996)
                     /gene="AGO2A"
                     /gene_synonym="AGO2A2"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

ctctctcaagttacagaatggatcgtgggaactaccgacgtggtggcggtcatgataacgccggcagaggtggaggtaaccgtggtcgaggacaaggccgtggtggtggaaggggtgagcctcagatcggaggtggaaggggtgggacccacatcggtggtgggtcccaaatgggtcagcctccacagcaatgggggaatcagccgaggggatcgggtcaataccagattcgtggtggggctccgcaaaatcaaccgggtcaaaactatccggttaatcagaatccgggtcgtggtggtacttgggtaaaccaaccggttcaacgtggtggtggtggtgggggtagcgcttggccacggccacaacagcagggtagtggcgttccaaacactacttggccccgaccaccaccgcagcaacagggtagtggtagtggagttgctaatgcttgggcccggccaccgccgcagaggagtcagcagcatggtggtggaaatcagcagcaggttgtggatcgttctcctcctcaatcttctgatcctgttcaagttgatctggggtccctgaaaattacagatcagagtccatcatctcgacaagaaagttccaaggaaaaacgtgtacctattgcacgacctgatactggaaaaattgctgtcaagtcaattgcgctgcttgctaatcattttcctgttagatttaatccccagtctaccattatgcattatgatgtggatattcaacagagggctgatgggaatcggcctgtgaagaagctaacaaacaagtctgttctccatatgataagagaaaagctgtgtgcggatgaccctacacgatttcccctagataaaactgcatatgacggtaagaagaacattttcagtgctgttcaacttcctactgggtgttttgctgtgaactggtctgacggggaagatgctaggttacgctcttatgacattaccatcaagcttgttgctgaattaaaactttgcaagttgaaagaatatttgagtggaagcctatcacatattcctcgtgatatactacaaggaatggagttggttatgaaagaaaatcctactaggtgcaggacttctgtaggtcgttgtttctactctaatgagcacctgcccgaccatgactttcgttttggtgttgctgcatatagaggttttcaacagagcctaaagcctacaaaaggagggcttgcgttgtgcttagattactcggtcttggcactccggaaaccaatgccagtgctagactttctgaaggaatatttaggagaatctaacgaaaataccttcaggaacaacattcgagctgcaaagggtgcattggttggtttgaaagtcagggtaattcatcgtcgtacaagccagaagtttcttattaagcagctaactgactgcaagactcgtgaaattacttttccccttgaagatcccgaaggcataaatcccccgagggatgttcttcttgttgactacttcagggataaatatcagcgcgagattcagttcaaggattttccttcattagatattggaaaaggtaataagaaaaactatgtcccgatggaattctgtgtcttggttgagggacaacggtatcccaaggaggatttggataaagatactgccttgtttctgaagaacatatcactagctcgaccccaagatagaaggcaggcaatttgtgaaatggtacgggctggagatgggccgtgcggggctgtcacccgcaattttgatattggagttgataggaacatgacccgtgttccgggtcgtattcttcctccccctgatttgaagctaggcggtcaaaatcgacttcctgtgaatgataaatgccagtggaaccttgttgggaaatctgtggtggaaggcaaggcgcttcagcgatgggctttgatagattttagcgctcaggatcgcaaacccttctttaggctacgagttgatgaatttgtcttcagattgaaagatcggtgcagaaaattgagtattaatatggaagaacctgctgtagtacatttcactgacatgcatgtactctctgaagttggcaaggttcataaacttctcgatggtgtggttaatgcagctaaacgggaaatcaatggtaaacttcaaatgatagtttgtgttatgacatcaaagcataatgggtacaaatatcttaaatgggtatctgagacacaaattggcgtagtaacccagtgttgcttgtctactaatgccaacaagggacaggatcaatatcttgcaaacctgtgtatgaagatcaatgcaaaacttgggggcagcaatatggaacttatggatagactccctaattttggaagagaagacaatgtcatgttcattggagctgatgttaatcatcctgctgcaaagaatgtgacatgtccatccatagcagccgttgttgccaccgtcaactggccagctgctaatagatatgctgctagagtttgtcctcaggttcacaggactgagaagattctagagtttggaaagatgtgtgcagacctagttcatacttataaggaaatcaactctgttaaaccaaacaaaattgttgttttccgtgatggtgtgagtgagggtcagtttgatatggtactcaacgaagagttgcttgatttggcgaaggctatatacgacagtaactatcaaccagcaattactcttgttgtggctcagaagagacaccatacacggctatttcctgagggtggccctgccaatgtgcctcctggtactgttgtggatacaataattgttcatccctctgattttgacttctatctttgcagccactttggagggttgggtaccagcaagccaactcactatcatgttctatgggatgataatggtttcaattctgacagcttacaaaagcttatatacaacatgtgtttcacttttgcgcggtgcactaaacctgtctcacttgttccaccagtttactatgcagaccttgttgcttataggggacggatgtttcaagaggtgcttatggagatgaactctcctagctctgcaacttcatcttctccaactgcctcatttcaacaaaaattttatgatttgcactctgatttgcagaacgtaatgtttttcgtctgatgccaccttgttgatctccttcaccattcccacactggggagggagatacggttgtctacattttggagatgcccatttccaaacgcatttgaacctcctttccgttggacattgcaaaggagtttgtgcgtgaatctaatggggtttcatgcttcttttacttttctattgtgtgagtttaatcattgacgtttgattgtgttttatctgctttggggggtctctggaggccactcccaaatatgtctactgcttaaattgtgtgatgttttgtattatgtatttctgagacgtgtcttaatattaatatatttgtgtttgagcta

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