LOCUS       NM_001104858            2460 bp    mRNA    linear   VRT 24-JUN-2020
DEFINITION  Oryzias latipes taste receptor, type 1, member 2a (tas1r2a), mRNA.
ACCESSION   NM_001104858
VERSION     NM_001104858.1
KEYWORDS    RefSeq.
SOURCE      Oryzias latipes (Japanese medaka)
  ORGANISM  Oryzias latipes
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Ovalentaria; Atherinomorphae; Beloniformes;
            Adrianichthyidae; Oryziinae; Oryzias.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Nango E, Akiyama S, Maki-Yonekura S, Ashikawa Y, Kusakabe Y,
            Krayukhina E, Maruno T, Uchiyama S, Nuemket N, Yonekura K, Shimizu
            M, Atsumi N, Yasui N, Hikima T, Yamamoto M, Kobayashi Y and
            Yamashita A.
  TITLE     Taste substance binding elicits conformational change of taste
            receptor T1r heterodimer extracellular domains
  JOURNAL   Sci Rep 6, 25745 (2016)
   PUBMED   27160511
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Ishimaru Y, Okada S, Naito H, Nagai T, Yasuoka A, Matsumoto I and
            Abe K.
  TITLE     Two families of candidate taste receptors in fishes
  JOURNAL   Mech. Dev. 122 (12), 1310-1321 (2005)
   PUBMED   16274966
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB200906.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB200906.2 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMD00066536, SAMD00066541
                                           [ECO:0000350]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Oryzias latipes"
                     /mol_type="mRNA"
                     /db_xref="taxon:8090"
                     /chromosome="7"
                     /map="7"
     gene            1..2460
                     /gene="tas1r2a"
                     /note="taste receptor, type 1, member 2a"
                     /db_xref="GeneID:100049505"
     CDS             1..2460
                     /gene="tas1r2a"
                     /codon_start=1
                     /product="taste receptor, type 1, member 2a precursor"
                     /protein_id="NP_001098328.1"
                     /db_xref="GeneID:100049505"
                     /translation="

MKVFLTLLYLFEGFLQAATQSTVQTSEFHLRGDYLIGGLFNIHYVAAANFQRPQAIDCSSKLFILPNYRRFQLMRFSVEEINNSSSLLPNISLGYQMFDHCSDIHSFPGIFKLLSVNDLIRPWEDASTGLPNAIGVVGPFTSTHALSIAPIFMTNLFPMVSYGCSGSVFSKKNLYPSFLRTVHSNKDVINAIVGIILNFNWRWVAFLYSDDDFGKDGLEQFKNKIEDSEICLAFYKAINIYTDYPQVFKQIEEQNIKVIVVFAPKVYAEAVVESAVQLNVTNKVWIADDGWSLNKKLPSMNGIQNIGTVLGVAQPVVTIPGFTDFIHSAISQTDGGDTEQKMFCNQKCNCSNLSVKTLLNADPSFSFPVYAAVYAIAHALHNTLRCGSDRCHKNITVHPHMILEELKKSNFTLLNQTVQFDENGDPKFGSLSVVFWNSSGNAEEVGSYHFQSSIHLSINKTKIKWFTNGEVPTSLCSPECPAGYAKKPNGMHKCCFTCEICKTNNYVNISEDPFKCISCKDTEWAAEGSTSCLPRLLEYVSLTDIGAVLTMVLEGLLLGLTVSVSVVFAINYNTPVVRSAGGPICFLNLGCLSLCSISVFFYFGVPTPSSCVLRYLPFILFYTVCLACFVVRSFQIVCIFKISAQIPNIHIWWVKYHGQWLLISAAFMFQALSLTISFCCDPPRPYNEMLWYPEQIILSCNLNIQASCFSLFLLVSLCCLCFIFSYMAKDLPKNYNEAKAITFCLLLLICTWVIFITEYMLYRGKYIQIFNALAVLSSLYSFLLWYFLPKCYIIIFKPDKNTQQYFQGLIQSYTKTISQ"

     sig_peptide     1..57
                     /gene="tas1r2a"
                     /inference="COORDINATES: ab initio prediction:SignalP:4.0"
     misc_feature    82..1419
                     /gene="tas1r2a"
                     /note="ligand-binding domain of the T1R taste receptor;
                     Region: PBP1_taste_receptor; cd06363"
                     /db_xref="CDD:380586"
     misc_feature    order(310..315,319..324,331..336,343..345,370..372,
                     439..441,448..450,460..465,655..657)
                     /gene="tas1r2a"
                     /note="dimer interface [polypeptide binding]; other site"
                     /db_xref="CDD:380586"
     misc_feature    order(418..420,487..495,637..639,868..870)
                     /gene="tas1r2a"
                     /note="ligand binding site [chemical binding]; other site"
                     /db_xref="CDD:380586"
     misc_feature    1414..1575
                     /gene="tas1r2a"
                     /note="Nine Cysteines Domain of family 3 GPCR; Region:
                     NCD3G; pfam07562"
                     /db_xref="CDD:429539"
     misc_feature    1633..2388
                     /gene="tas1r2a"
                     /note="type 1 taste receptor subtype 2a and similar
                     proteins, member of the class C of seven-transmembrane G
                     protein-coupled receptors; Region: 7tmC_TAS1R2a-like;
                     cd15287"
                     /db_xref="CDD:320414"
     misc_feature    1633..1710
                     /gene="tas1r2a"
                     /note="TM helix 1 [structural motif]; Region: TM helix 1"
                     /db_xref="CDD:320414"
     misc_feature    order(1642..1644,1651..1656,1663..1668,1672..1677,
                     1777..1779,1789..1791,1798..1803,1825..1827,1837..1839)
                     /gene="tas1r2a"
                     /note="putative dimer interface [polypeptide binding];
                     other site"
                     /db_xref="CDD:320414"
     misc_feature    1744..1809
                     /gene="tas1r2a"
                     /note="TM helix 2 [structural motif]; Region: TM helix 2"
                     /db_xref="CDD:320414"
     misc_feature    order(1804..1806,1840..1845,1852..1857,1864..1866,
                     2104..2106,2119..2121,2128..2133,2140..2142,2242..2244,
                     2251..2256,2263..2265,2275..2277,2299..2304,2311..2313,
                     2320..2322,2332..2334)
                     /gene="tas1r2a"
                     /note="putative allosteric modulator binding site
                     [chemical binding]; other site"
                     /db_xref="CDD:320414"
     misc_feature    1834..1908
                     /gene="tas1r2a"
                     /note="TM helix 3 [structural motif]; Region: TM helix 3"
                     /db_xref="CDD:320414"
     misc_feature    1975..2037
                     /gene="tas1r2a"
                     /note="TM helix 4 [structural motif]; Region: TM helix 4"
                     /db_xref="CDD:320414"
     misc_feature    2107..2187
                     /gene="tas1r2a"
                     /note="TM helix 5 [structural motif]; Region: TM helix 5"
                     /db_xref="CDD:320414"
     misc_feature    2209..2280
                     /gene="tas1r2a"
                     /note="TM helix 6 [structural motif]; Region: TM helix 6"
                     /db_xref="CDD:320414"
     misc_feature    2293..2370
                     /gene="tas1r2a"
                     /note="TM helix 7 [structural motif]; Region: TM helix 7"
                     /db_xref="CDD:320414"
     exon            1..179
                     /gene="tas1r2a"
                     /inference="alignment:Splign:2.1.0"
     exon            180..477
                     /gene="tas1r2a"
                     /inference="alignment:Splign:2.1.0"
     exon            478..1203
                     /gene="tas1r2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1410
                     /gene="tas1r2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1531
                     /gene="tas1r2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..2460
                     /gene="tas1r2a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

atgaaagtcttcctgactctcctgtatttgtttgagggtttcctgcaggctgcaactcagagcaccgttcagacctcagagtttcatctgagaggagattatttgataggtggactttttaacattcattatgtcgccgcagctaattttcaaagaccacaagccatcgactgctccagtaaacttttcatactcccaaattatcggaggtttcagctgatgagattctctgtggaggaaatcaacaactcctcctccctcttgcccaacatatctcttggctatcagatgtttgaccactgctcggatatacacagctttccagggattttcaaactcctctcagtcaatgacttgatccgaccctgggaagacgcttccacgggtttgcctaacgcgattggagtggttggtcctttcacaagcactcatgccctctccatagcccctattttcatgacaaatctctttcctatggtcagttatggatgctctggctcagtgttttccaaaaaaaatctttatccctctttcctgagaacagtgcattctaataaagatgttataaatgccattgttgggatcatactgaactttaattggcgctgggttgccttcttatacagtgatgatgattttggcaaagatgggctggagcagtttaaaaacaagattgaagacagtgaaatctgcctggcattctacaaagcaatcaacatttatacagattacccccaagtgttcaagcagatagaggaacagaacataaaggtcattgttgtatttgctccaaaagtctatgctgaagctgttgttgagtcggcagttcagctgaacgtcacaaataaggtgtggatcgcagacgatggttggtccttgaacaaaaagctgccgagcatgaatggaatccaaaatatcgggacggtactcggcgtggctcaaccagttgtgaccatacctggttttactgattttatccattctgcaataagtcagacagacggtggagacacagaacagaaaatgttttgtaatcagaagtgtaactgcagtaacctgagtgtaaaaactcttcttaatgccgatccatctttctctttccccgtttacgctgctgtttatgccattgcacatgccctacacaacactttgaggtgcggttctgacaggtgccataaaaatattacagttcacccacacatgattctggaggagttgaagaaatccaacttcaccctgctaaaccaaactgtgcagtttgatgagaatggggatcccaaatttggatccttgtcagtagttttctggaacagcagtgggaatgcagaggaagtcggctcttatcattttcaatcatcgattcatctctccatcaataagaccaaaattaagtggttcacaaatggagaggtaccgacttcattgtgttccccagagtgtccagccggatatgcaaaaaagccaaatggaatgcacaaatgctgcttcacttgtgaaatctgtaaaactaataactacgtaaatatttcagaagatcccttcaagtgcatcagctgtaaagacaccgagtgggctgcagaaggaagtacatcatgcctcccaaggctgctggagtatgtgtcattgacagacattggtgctgtgttaaccatggttttggaggggctcctgctgggcctcacagtaagtgtgtctgttgtcttcgccatcaactacaacacacctgtggtcagatccgccggaggaccgatttgcttcctgaatcttggttgcctcagcctgtgtagcatcagtgttttcttttactttggtgttcccacaccttcttcttgtgtattaagataccttccgtttattttgttttacaccgtctgcttggcatgttttgtggtgcgctcttttcagattgtctgcattttcaaaatatctgcacaaatccccaacatccacatttggtgggtaaaataccacggacagtggcttctcatcagcgcggcgttcatgttccaggcactctcacttaccattagcttttgctgtgatcctccacgtccctacaatgaaatgttgtggtatccagagcaaataatactcagctgtaacttgaacattcaagcatcttgtttttctttgtttttacttgtgtcactgtgctgtctttgcttcattttctcttacatggcgaaagatctccccaaaaattacaacgaagccaaagcgataactttctgtcttctccttctcatctgcacctgggtcatctttataaccgagtacatgctttatcgcgggaaatacatccagatttttaatgctctggcagttctatcaagtctgtactcttttctgttgtggtatttcctcccaaaatgttacatcattatctttaaacccgacaaaaacacacagcagtactttcaaggccttattcagagttacaccaaaacaattagccagtag

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