LOCUS       NM_001412813            4228 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens zinc fingers and homeoboxes 2 (ZHX2), transcript
            variant 20, mRNA.
ACCESSION   NM_001412813
VERSION     NM_001412813.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Creasy,K.T., Ren,H., Jiang,J., Peterson,M.L. and Spear,B.T.
  TITLE     Elongation of very long chain fatty acids-3 (Elovl3) is activated
            by ZHX2 and is a regulator of cell cycle progression
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 325 (6), G582-G592 (2023)
   PUBMED   37847682
  REMARK    GeneRIF: Elongation of very long chain fatty acids-3 (Elovl3) is
            activated by ZHX2 and is a regulator of cell cycle progression.
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Tan,S., Wang,Z., Li,N., Guo,X., Zhang,Y., Ma,H., Peng,X., Zhao,Y.,
            Li,C., Gao,L., Li,T., Liang,X. and Ma,C.
  TITLE     Transcription factor Zhx2 is a checkpoint that programs macrophage
            polarization and antitumor response
  JOURNAL   Cell Death Differ 30 (9), 2104-2119 (2023)
   PUBMED   37582865
  REMARK    GeneRIF: Transcription factor Zhx2 is a checkpoint that programs
            macrophage polarization and antitumor response.
REFERENCE   3  (bases 1 to 4228)
  AUTHORS   Ding,R., Shi,H., Guo,Y., Zeng,W. and Fan,J.
  TITLE     Zinc fingers and homeoboxes 2 inhibition could suppress the
            proliferation of ovarian cancer cells by apoptosis pathway
  JOURNAL   Clin Transl Oncol 25 (7), 2116-2126 (2023)
   PUBMED   36746874
  REMARK    GeneRIF: Zinc fingers and homeoboxes 2 inhibition could suppress
            the proliferation of ovarian cancer cells by apoptosis pathway.
REFERENCE   4  (bases 1 to 4228)
  AUTHORS   Xie,H., Zhou,J., Liu,X., Xu,Y., Hepperla,A.J., Simon,J.M., Wang,T.,
            Yao,H., Liao,C., Baldwin,A.S., Gong,K. and Zhang,Q.
  TITLE     USP13 promotes deubiquitination of ZHX2 and tumorigenesis in kidney
            cancer
  JOURNAL   Proc Natl Acad Sci U S A 119 (36), e2119854119 (2022)
   PUBMED   36037364
  REMARK    GeneRIF: USP13 promotes deubiquitination of ZHX2 and tumorigenesis
            in kidney cancer.
REFERENCE   5  (bases 1 to 4228)
  AUTHORS   Tan,S., Guo,X., Li,M., Wang,T., Wang,Z., Li,C., Wu,Z., Li,N.,
            Gao,L., Liang,X. and Ma,C.
  TITLE     Transcription factor Zhx2 restricts NK cell maturation and
            suppresses their antitumor immunity
  JOURNAL   J Exp Med 218 (9) (2021)
   PUBMED   34279541
  REMARK    GeneRIF: Transcription factor Zhx2 restricts NK cell maturation and
            suppresses their antitumor immunity.
REFERENCE   6  (bases 1 to 4228)
  AUTHORS   Liu,G., Clement,L.C., Kanwar,Y.S., Avila-Casado,C. and Chugh,S.S.
  TITLE     ZHX proteins regulate podocyte gene expression during the
            development of nephrotic syndrome
  JOURNAL   J Biol Chem 281 (51), 39681-39692 (2006)
   PUBMED   17056598
  REMARK    GeneRIF: ZHX proteins 1, 2 and 3 are major transcriptional
            mediators of podocyte disease
            Erratum:[J Biol Chem. 2007 Apr 27;282(17):13140]
REFERENCE   7  (bases 1 to 4228)
  AUTHORS   de Andrade,T.G., Peterson,K.R., Cunha,A.F., Moreira,L.S.,
            Fattori,A., Saad,S.T. and Costa,F.F.
  TITLE     Identification of novel candidate genes for globin regulation in
            erythroid cells containing large deletions of the human beta-globin
            gene cluster
  JOURNAL   Blood Cells Mol Dis 37 (2), 82-90 (2006)
   PUBMED   16952470
  REMARK    GeneRIF: ZHX2, a transcriptional repressor, may participate in
            globin gene regulation.
REFERENCE   8  (bases 1 to 4228)
  AUTHORS   Lim,J., Hao,T., Shaw,C., Patel,A.J., Szabo,G., Rual,J.F.,
            Fisk,C.J., Li,N., Smolyar,A., Hill,D.E., Barabasi,A.L., Vidal,M.
            and Zoghbi,H.Y.
  TITLE     A protein-protein interaction network for human inherited ataxias
            and disorders of Purkinje cell degeneration
  JOURNAL   Cell 125 (4), 801-814 (2006)
   PUBMED   16713569
REFERENCE   9  (bases 1 to 4228)
  AUTHORS   Kawata,H., Yamada,K., Shou,Z., Mizutani,T. and Miyamoto,K.
  TITLE     The mouse zinc-fingers and homeoboxes (ZHX) family; ZHX2 forms a
            heterodimer with ZHX3
  JOURNAL   Gene 323, 133-140 (2003)
   PUBMED   14659886
REFERENCE   10 (bases 1 to 4228)
  AUTHORS   Kawata,H., Yamada,K., Shou,Z., Mizutani,T., Yazawa,T., Yoshino,M.,
            Sekiguchi,T., Kajitani,T. and Miyamoto,K.
  TITLE     Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX
            family, functions as a transcriptional repressor
  JOURNAL   Biochem J 373 (Pt 3), 747-757 (2003)
   PUBMED   12741956
  REMARK    GeneRIF: identification as a transcriptional repressor
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC016405.8 and AC104316.3.
            
            Summary: The members of the zinc fingers and homeoboxes gene family
            are nuclear homodimeric transcriptional repressors that interact
            with the A subunit of nuclear factor-Y (NF-YA) and contain two
            C2H2-type zinc fingers and five homeobox DNA-binding domains. This
            gene encodes member 2 of this gene family. In addition to forming
            homodimers, this protein heterodimerizes with member 1 of the zinc
            fingers and homeoboxes family. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.1929108.1,
                                           SRR14372079.3553368.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2157511 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-292               AC016405.8         114417-114708       c
            293-355             AC016405.8         32824-32886         c
            356-2959            AC104316.3         73535-76138
            2960-4228           AC104316.3         95485-96753
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.13"
     gene            1..4228
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="zinc fingers and homeoboxes 2"
                     /db_xref="GeneID:22882"
                     /db_xref="HGNC:HGNC:18513"
                     /db_xref="MIM:609185"
     exon            1..292
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /inference="alignment:Splign:2.1.0"
     exon            293..355
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /inference="alignment:Splign:2.1.0"
     exon            356..2959
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    479..481
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="upstream in-frame stop codon"
     CDS             575..2986
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="isoform 2 is encoded by transcript variant 20; zinc
                     fingers and homeoboxes protein 2; transcription factor
                     ZHX2; AFP regulator 1; regulator of AFP; alpha-fetoprotein
                     regulator 1; zinc finger and homeodomain protein 2"
                     /codon_start=1
                     /product="zinc fingers and homeoboxes protein 2 isoform 2"
                     /protein_id="NP_001399742.1"
                     /db_xref="GeneID:22882"
                     /db_xref="HGNC:HGNC:18513"
                     /db_xref="MIM:609185"
                     /translation="

MASKRKSTTPCMVRTSQVVEQDVPEEVDRAKEKGIGTPQPDVAKDSWAAELENSSKENEVIEVKSMGESQSKKLQGGYECKYCPYSTQNLNEFTEHVDMQHPNVILNPLYVCAECNFTTKKYDSLSDHNSKFHPGEANFKLKLIKRNNQTVLEQSIETTNHVVSITTSGPGTGDSDSGISVSKTPIMKPGKPKADAKKVPKKPEEITPENHVEGTARLVTDTAEILSRLGGVELLQDTLGHVMPSVQLPPNINLVPKVPVPLNTTKYNSALDTNATMINSFNKFPYPTQAELSWLTAASKHPEEHIRIWFATQRLKHGISWSPEEVEEARKKMFNGTIQSVPPTITVLPAQLAPTKVTQPILQTALPCQILGQTSLVLTQVTSGSTTVSCSPITLAVAGVTNHGQKRPLVTPQAAPEPKRPHIAQVPEPPPKVANPPLTPASDRKKTKEQIAHLKASFLQSQFPDDAEVYRLIEVTGLARSEIKKWFSDHRYRCQRGIVHITSESLAKDQLAIAASRHGRTYHAYPDFAPQKFKEKTQGQVKILEDSFLKSSFPTQAELDRLRVETKLSRREIDSWFSERRKLRDSMEQAVLDSMGSGKKGQDVGAPNGALSRLDQLSGAQLTSSLPSPSPAIAKSQEQVHLLRSTFARTQWPTPQEYDQLAAKTGLVRTEIVRWFKENRCLLKTGTVKWMEQYQHQPMADDHGYDAVARKATKPMAESPKNGGDVVPQYYKDPKKLCEEDLEKLVTRVKVGSEPAKDCLPAKPSEATSDRSEGSSRDGQGSDENEESSVVDYVEGSLLELLC"

     misc_feature    653..805
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y6X8.1);
                     Region: Interaction with EFNB1.
                     /evidence=ECO:0000250|UniProtKB:Q8C0C0"
     misc_feature    683..685
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:23186163,
                     ECO:0007744|PubMed:24275569; propagated from
                     UniProtKB/Swiss-Prot (Q9Y6X8.1); phosphorylation site"
     misc_feature    890..1048
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="Zinc-fingers and homeoboxes C2H2 finger domain;
                     Region: zf_C2H2_ZHX; pfam18387"
                     /db_xref="CDD:465737"
     misc_feature    1064..1186
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y6X8.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1157..1648
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y6X8.1);
                     Region: Required for homodimerization.
                     /evidence=ECO:0000269|PubMed:12741956"
     misc_feature    1193..1195
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9Y6X8.1); phosphorylation site"
     misc_feature    1361..2065
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y6X8.1);
                     Region: Required for interaction with NFYA.
                     /evidence=ECO:0000269|PubMed:12741956"
     misc_feature    1361..1912
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y6X8.1);
                     Region: Required for repressor activity.
                     /evidence=ECO:0000269|PubMed:12741956"
     misc_feature    1388..1537
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="Homeodomain; DNA binding domains involved in the
                     transcriptional regulation of key eukaryotic developmental
                     processes; may bind to DNA as monomers or as homo- and/or
                     heterodimers, in a sequence-specific manner; Region:
                     homeodomain; cd00086"
                     /db_xref="CDD:238039"
     misc_feature    1523..1912
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y6X8.1);
                     Region: Required for nuclear localization.
                     /evidence=ECO:0000269|PubMed:12741956"
     misc_feature    1784..1909
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y6X8.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1904..2062
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="Homeodomain; DNA binding domains involved in the
                     transcriptional regulation of key eukaryotic developmental
                     processes; may bind to DNA as monomers or as homo- and/or
                     heterodimers, in a sequence-specific manner; Region:
                     homeodomain; cd00086"
                     /db_xref="CDD:238039"
     misc_feature    order(1907..1909,2027..2029,2036..2041,2048..2050)
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="specific DNA base contacts [nucleotide binding];
                     other site"
                     /db_xref="CDD:238039"
     misc_feature    2183..2320
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="Homeodomain; Region: HOX; smart00389"
                     /db_xref="CDD:197696"
     misc_feature    2477..2626
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="Homeodomain; DNA binding domains involved in the
                     transcriptional regulation of key eukaryotic developmental
                     processes; may bind to DNA as monomers or as homo- and/or
                     heterodimers, in a sequence-specific manner; Region:
                     homeodomain; cd00086"
                     /db_xref="CDD:238039"
     exon            2960..4228
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4200..4205
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="hexamer: AATAAA"
     regulatory      4212..4217
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="hexamer: ACTAAA"
     polyA_site      4225
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
     polyA_site      4228
                     /gene="ZHX2"
                     /gene_synonym="AFR1; RAF"
                     /note="major polyA site"
ORIGIN      

atgtgggttttggcgtagattccccactgatcgaggcattttttttccctttttttttcctttttttttttcttttaaaaattttggccatcgttctccgtacgggggctttttctgtctgtctgtctggctggcaggctggctttccccctctttcccacggagcccgagccgggcgcccggtggggagtggggagtgggtggggggagccagcagagttccattttggaacgcccgtgccgcgtctccgcgttcccagcccgggtccccgcgttcacagccccagcgcaggtctggatgtaccgactgcttttggaataaaaagattcccaggatgtgagcaacacgggaccgatatgatgcttcctggtgtgtttagtggttggtgccattccaattttctgtgctgaaatcattctgaaaactcaaacagtagacttcagcacacaaggaaagccaaagccatttgagggggaataaagccaaaagcctttcaccttattcgttccaagaatctcaccgccccctccttatccccctccaaaaataagccattgcacacagacaggcagcatggctagcaaacgaaaatctacaactccatgcatggttcggacatcacaagtagtagaacaagatgtgcccgaggaagtagacagggccaaagagaaaggaatcggcacaccacagcctgacgtggccaaggacagttgggcagcagaacttgaaaactcttccaaagaaaacgaagtgatagaggtgaaatctatgggggaaagccagtccaaaaaactccaaggtggttatgagtgcaaatactgcccctactccacgcaaaacctgaacgagttcacggagcatgtcgacatgcagcatcccaacgtgattctcaaccccctctacgtgtgtgcagaatgtaacttcacaaccaaaaagtacgactccctatccgaccacaactccaagttccatcccggggaggccaacttcaagctgaagttaattaaacgcaataatcaaactgtcttggaacagtccatcgaaaccaccaaccatgtcgtgtccatcaccaccagtggccctggaactggtgacagtgattctgggatctcggtgagtaaaacccccatcatgaagcctggaaaaccaaaagcggatgccaagaaggtgcccaagaagcccgaggagatcacccccgagaaccacgtggaagggaccgcccgcctggtgacagacacagctgagatcctctcgagactcggcggggtggagctcctccaagacacattaggacacgtcatgccttctgtacagctgccaccaaatatcaaccttgtgcccaaggtccctgtcccactaaatactaccaaatacaactctgccctggatacaaatgccacgatgatcaactctttcaacaagtttccttacccgacccaggctgagttgtcctggctgacagctgcctccaaacacccagaggagcacatcagaatctggtttgccacccagcgcttaaagcatggcatcagctggtccccagaagaggtggaggaggcccggaagaagatgttcaacggcaccatccagtcagtacccccgaccatcactgtgctgcccgcccagttggcccccacaaaggtgacgcagcccatcctccagacggctctaccgtgccagatcctcggccagactagcctggtgctgactcaggtgaccagcgggtcaacaaccgtctcttgctcccccatcacacttgccgtggcaggagtcaccaaccatggccagaagagacccttggtgactccccaagctgcccccgaacccaagcgtccacacatcgctcaggtgccagagcccccacccaaggtggccaaccccccgctcacaccagccagtgaccgcaagaagacaaaggagcagatagcacatctcaaggccagctttctccagagccagttccctgacgatgccgaggtttaccggctcatcgaggtgactggccttgccaggagcgagatcaagaagtggttcagtgaccaccgatatcggtgtcaaaggggcatcgtccacatcaccagcgaatcccttgccaaagaccagttggccatcgcggcctcccgacacggtcgcacgtatcatgcgtacccagactttgccccccagaagttcaaagagaaaacacagggtcaggttaaaatcttggaagacagctttttgaaaagttcttttcctacccaagcagaactggatcggctaagggtggagaccaagctgagcaggagagagatcgactcctggttctcggagaggcggaagcttcgagacagcatggaacaagctgtcttggattccatggggtctggcaaaaaaggccaagatgtgggagcccccaatggtgctctgtctcgactcgaccagctctccggtgcccagttaacaagttctctgcccagcccttcgccagcaattgcaaaaagtcaagaacaggttcatctcctgaggagcacgtttgcaagaacccagtggcctactccccaggagtacgaccagttagcggccaagactggcctggtccgaactgagattgtgcgttggttcaaggagaacagatgcttgctgaaaacgggaaccgtgaagtggatggagcagtaccagcaccagcccatggcagatgatcacggctacgatgccgtagcaaggaaagcaacaaaacccatggccgagagcccaaagaacgggggtgatgtggttccacaatattacaaggaccccaaaaagctctgcgaagaggacttggagaagttggtgaccagggtaaaagtaggcagcgagccagcaaaagactgtttgccagcaaagccctcagaggccacctcagaccggtcagagggcagcagccgggacggccagggtagcgacgagaacgaggagtcgagcgttgtggattacgtggagggaagtctgttagaactgctgtgctgatcaacgggacgctccgtctttgaagaaagaagagatggtctctccccagccatgggccacccttgccagtgactccaagtggaactacttagctcgcgtgtgcctggagggtgcgggaagtccagcgactctcagacgcacctcccagaggaccggtgggaattgttcatagtgccaaagtcctactactgcgttttcaatgggtccttgtacatagtttgctcctctgccctagccctcacctcttgctatactggaaccgatttgtacaatgtgggaattttgttacctttttaatcaagggcaacttccttttccagcactaccattgtaaggtttttttcaggagggagggctaaccaccttgcttttctcttttctctttttcttttttttatttttgttttattaatttggggaaaggggtgttagcattagtgccatgatatctactggattttaagtagggagactttatttttaaaggtaggttgaaatttgggagatttctcggcaggaagggctgaaatccaggcccctgtctcaacttggagagaggtgacagacggcagatcttccaaatcaaattcctttccagttcttcccctggctgcctttttgggggtccctgccttagccccacacaaggctttctgaactgccaagaggggatctggcttctcaactgctcggcctcttgggccaggctgtgcccagccagccctgggagaactgggtagcaggtggctgacttctttaagcacctttctaaataccagcagaagaggctcccgcctctgttagcatgatcagtactattgtgacattaaaacaacaacaataagatcttcctatctggagggtacagaggtgaatggctttggttttcatttctcttcttcactgccttttctcggtgtggtatttgacaagattttagctcaaagcctcaccatgaattgattttttttgtttgtgtgtgtgtttgttttgggacaattttagatacctgagtgcactttttcagttagtcctaacttttaaaagaaggaaaaccaagagacatatctggtgtacgtgttgcagtatgaactctggttgcaatccctccccgtcccacactgccccccatttgagtacaccgcacaagtcaaacgctaggaagtttgaataaaaccaatttttctaacttgttgctcatttgttgtaactcaataaagcaaagactaaacatttttataa

//